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1.
THE PENETRATION OF THE MEMBRANE OF BRAIN MITOCHONDRIA BY ANIONS 总被引:1,自引:0,他引:1
The permeability of the membrane of rat brain non-synaptosomal mitochondria, towards inorganic and substrate anions, was assessed by measuring the rate of swelling that occurred when mitochondria were suspended in an iso-osmotic solution of a permeant anion, in the presence of a permeant cation such as NH+4 or K+ in the presence or absence of valinomycin. In NH+4-phosphate swelling was higher than it was in KCI or K+-phosphate, which showed the prevalence of the mechanism of phosphate transport previously demonstrated in liver mitochondria. The entry of succinate and L-malate seemed to require the presence in the inner mitochondrial membrane of specific carriers. as previously postulated for liver mitochondria, but the rate of swelling of brain mitochondria was lower than that of liver organelles. In K+-succinate, in the presence of antimycin, added ATP induced swelling and this was attributable to the simultaneous permeation both of the anion and the cation. Fumarate did not penetrate into brain mitochondria. Practically no swelling was recorded in NH+4 or K+-citrate, which indicated that this anion penetrated poorly into the isolated brain mitochondria even in the presence of malate. Swelling occurred in NH+4-L-glutamate in the presence of rotenone, and the entry of this anion seemed to follow a gradient of concentration although the presence of a specific translocator in the inner mitochondrial membrane might be concerned. The entry of glutamate was independent of that of phosphate and N-ethylmaleimide appeared to be a specific inhibitor of this entry. Swelling in K+-L-glutamate, in the presence of rotenone, was enhanced by the addition of valinomycin or ATP but in the latter case when osmotic equilibrium was reached swelling was not reversed by oligomycin. In conclusion, the lesser extent of swelling of isolated brain mitochondria compared with liver mitochondria could be attributed to the heterogeneity of the populations of these organelles, each population possessing its own characteristics of membrane permeability. Observations of electron micrographs of brain mitochondria incubated in iso-osmotic substrate anions confirmed the heterogeneous rate of swelling of these particles. 相似文献
2.
Xavier Roucou Stéphen Manon Martine Guérin 《Journal of bioenergetics and biomembranes》1995,27(3):353-362
The mode of action of propranolol, chlorpromazine, and quinine, three cationic drugs inhibiting swelling of yeast mitochondria in potassium acetate, was investigated by looking at their effect on fluorescent probes of the polar heads and of the nonpolar moiety of the membranes, under inhibitory conditions of swelling. As expected, propranolol and chlorpromazine exhibited specificity for anionic phospholipids since they increased the binding of the anionic probe 1-anilino 8-naphthalenesulfonate (ANS). Although propranolol did not release 1,6-diphenyl-1,3,5-hexatriene (DPH) from the hydrophobic moiety of the membrane, it increased the excimer/ monomer fluorescence ratio of 10-(1-pyrene)decanoate, suggesting that it induced a limitation in the movements of the aliphatic chains of phospholipids. Opposite to propranolol, chlorpromazine removed DPH from the membrane, suggesting that it bound essentially to the hydrophobic moiety. However, chloramphenicol, which was also able to remove DPH but did not increase the binding of ANS, did not inhibit swelling. Inhibition by chlorpromazine therefore appeared to be related to its binding to the hydrophobic moiety of anionic phospholipids. Quinine had no effect on membrane properties: at inhibitory concentrations of swelling in potassium acetate, it did not inhibit swelling in ammonium phosphate (mediated by the phosphate/H+ cotransporter), whereas propranolol and chlorpromazine did, suggesting a more specific effect of quinine on (a) protein(s) involved in the K+/H+ exchange. Dicyclohexylcarbodiimide (DCCD), which irreversibly inhibits the swelling in potassium acetate, bound to ethanolamine heads; despite this effect, DCCD had no major consequences on the binding of the probes. Consequently, propranolol and chlorpromazine are of no help for characterizing protein(s) catalyzing the K+/H+ exchange, although their effect on lipids seems to involve limited zones of the inner mitochondrial membrane. Quinine and DCCD, although they also bind to lipids, may inhibit the activity by acting on a limited number of proteins. 相似文献
3.
Tightly coupled respiring corn mitochondria (Zea mays L.) respond to calcium addition with a transitory respiratory increase, proton extrusion, and Ca2+ binding. The extent of response is dependent upon the level of endogenous phosphate, and a large sustained respiratory increase can be obtained with addition of phosphate. However, calcium does not act as a permeant cation in that it will not penetrate with acetate. It appears that the transitory respiratory increase must be linked to the uptake of a calcium phosphate complex, but there is no evidence that transport of the complex serves to produce an electrophoretic calcium uniport. It is believed that calcium phosphate transport in corn is a constitutive property, and not produced by membrane damage. 相似文献
4.
Swelling of nonenergized heart mitochondria suspended in acetate salts appears to depend on the activity of an endogenous cation/H+ exchanger. Passive swelling in acetate shows a characteristic cation selectivity sequence of Na+ >Li+ >K+, Rb+, Cs+, or tetramethylammonium, a sharp optimum at pH 7.2–7.3, activation by Ca2+, and loss of activity on aging which can be related to loss of endogenous K+. The reaction is nearly insensitive to either addition of exogenous Mg2+ or removal of membrane Mg2+ with EDTA. Each of these characteristics of passive swelling in acetate salts is duplicated in chloride media when tripropyltin is added to induce Cl?/OH? exchange. In contrast to nonenergized mitochondria, swelling of respiring mitochondria has been postulated to depend on electrophoretic uptake of cations in response to an interior negative membrane potential. Respiration-dependent swelling in acetate shows an indistinct cation selectivity sequence with Li+ and Na+ supporting higher rates of swelling at higher efficiency than K+, Rb+, and Cs+. The high rates of respiration-dependent swelling in Li+ and Na+ are inhibited by low levels of exogenous Mg2+ (Ki of 5–10 μm), but a significant swelling with almost no cation selectivity persists in the presences of 2 mm Mg2+. Removal of membrane Mg2+ by addition of EDTA strongly activates the rate of respiration-dependent swelling and converts a sigmoid dependency of swelling rate on Li+ concentration to a hyperbolic one with a Km of about 14 mm Li+. The cation selectivity and Mg2+ dependence of the reaction induced in chloride salts by tripropyltin are identical to these properties in acetate. Energy-dependent swelling in acetate shows optimum activity at pH 6.5 which appears related to the availability of free acetic acid, since the corresponding reaction induced in chloride shows a broad optimum at about pH 7.5. These studies support the concept that monovalent cations enter nonenergized mitochondria by electroneutral exchange with protons but penetrate respiring mitochondria by electrophoretic movement through one or more uniport pathways. They further suggest that both a Mg2+-sensitive uniport with high activity for Na+ and Li+ and a Mg2+-insensitive pathway with little cation discrimination are available in the membrane. 相似文献
5.
K D Garlid 《Biochemical and biophysical research communications》1978,83(4):1450-1455
Matrix swelling induces a rapid, transient, energy-independent potassium efflux in rat liver mitochondria. Swelling-induced K+-loss is electroneutral; therefore it does not reflect electrophoretic diffusion secondary to increased membrane permeability. Matrix swelling unmasks an endogenous transport mechanism in the mitochondrial membrane, providing a valuable experimental approach to the study of K+ transport in mitochondria. 相似文献
6.
Igor A. Skulskii Margarita V. Savina Vadim V. Glasunov Nils-Erik L. Saris 《The Journal of membrane biology》1978,44(2):187-194
Summary The distribution of Tl+ between rat liver mitochondria and the medium was studied; millimolar or smaller concentrations of Tl+ were labeled with204Tl. The Tl+ distribution responded to transient diffusion potentials in a way that indicated electrophoretic movements of Tl+. The diffusion potentials were induced by efflux of K+ in response to addition of valinomycin to nonrespiring mitochondria suspended in a medium with low concentrations of K+ or by efflux of H+ induced by making the medium more alkaline in the presence of a protonophorous (proton-conducting) uncoupling agent. Changes in membrane potential induced by valinomycin were followed with the aid of safranine. Tl+ brought about collapse of the diffusion potential. It is concluded that Tl+ is able to penetrate the mitochondrial membrane electrophoretically. 相似文献
7.
Ion transport by heart mitochondria. XXII. Spontaneous, energy-linked accumulation of acetate and phosphate salts of monovalent cations 总被引:4,自引:0,他引:4
G P Brierley M Jurkowitz K M Scott A J Merola 《Archives of biochemistry and biophysics》1971,147(2):545-556
The accumulation of monovalent cations by isolated beef heart mitochondria has been studied by evaluating the efficiency of energy-dependent osmotic swelling. Extensive osmotic swelling occurs spontaneously when isolated heart mitochondria are suspended in 0.1 m acetate or phosphate salts. The swelling and ion uptake depend on either respiration or the presence of exogenous ATP, and the initial rate of swelling is proportional to the initial rate of respiration or ATP hydrolysis, respectively. The efficiency of the reaction varies somewhat from preparation to preparation but approaches a limit of about 2 cations accumulated per pair of electrons traversing a phosphorylation site. All monovalent cations tested support the reaction, but the most efficient energy-dependent swelling occurs with K+. Weak acid anions are required for the ion accumulation and swelling and the reaction appears to depend on the amount of free acid available in the suspension. Permeant strong acid anions, such as NO3−, fail to support the swelling reaction in the presence of energy. Valinomycin increases both the amount and the efficiency of ion uptake under these conditions. Mg2+ decreases both of these values whereas p-chloromercuriphenyl sulfonate increases both. These responses are discussed in terms of current models of mitochondrial ion transport. 相似文献
8.
A comparative study of the fat body of diapausing and non-diapausing larvae of the corn borer, Diatraea grandiosella, was undertaken using the electron microscope and the oxygen electrode. The electron microscopic results showed a shift from a synthetic to a storage function taking place in a 1 to 2 day period during the final instar of non-diapausing larvae, and in a 4 to 8 day period in that of pre-diapausing larvae. This transition was characterized by a decrease in the number of mitochondria and amount of rough endoplasmic reticulum, and by an increase in the number of proteinaceous granules and lysosomes. In vitro measurements using the oxygen electrode showed that the fat body is a normal aerobic respiratory tissue. The tissue reacted in a predictable manner to inhibitors of oxidative metabolism, including malonate, rotenone, oligomycin, and antimycin, and to the uncoupler, dinitrophenol. During the last instar the observed decrease in the respiratory rate of the fat body coincided with the observed ultrastructural changes in its cells. The fat body of 75 day old environmentally induced and juvenile hormone induced diapausing larvae consumed 90% and 78% less oxygen, respectively than that of 14 day old non-diapausing larvae. 相似文献
9.
Naoki Kamo Makoto Muratsugu Ruji Hongoh Yonosuke Kobatake 《The Journal of membrane biology》1979,49(2):105-121
Summary The membrane potential of mitochondria was estimated from the accumulation of tetraphenyl phosphonium (TPP+), which was determined with the TPP+-selective electrode developed in the present study. The preparation and some operational parameters of the electrode were described. The kinetics for uptake by mitochondria of TPP+ and DDA+ (dibenzyldimethyl ammonium) were analyzed, and it was found that TPP+ permeated the mitochondrial membrane about 15 times faster than DDA+. The final amounts of accumulation of TPP+ and DDA+ by mitochondria were approximately equal. For the state-4 mitochondria, the membrane potential was about 180 mV (interior negative). Simulataneous measurements of TPP+-uptake and oxygen consumption showed that the transition between states 3 and 4 was detectable by use of the TPP+-electrode. After the TPP+-electrode showed that state-4 was reached, the extramitochondrial phosphorylation potential was measured. The difference in pH across the membrane was measured from the distribution of permeant anion, acetate, so as to calculate the proton electrochemical potential. The ratio of extra-mitochondrial phosphorylation potential to proton electro-chemical potential,n was close to 3. This value ofn was also found to be 3 when ATP was hydrolyzed under the condition that the respiratory chain was arrested. The implication thatn=3 was discussed. 相似文献
10.
A. G. Shugaev I. M. Andreev E. I. Vyskrebentseva 《Russian Journal of Plant Physiology》2005,52(2):182-188
Mitochondria isolated from sugar beet (Beta vulgaris L.) taproot were shown to swell spontaneously after the transfer from a sucrose-containing isolation medium to isoosmotic potassium chloride solutions. The kinetics of this process was strongly retarded after the replacement of potassium with sodium in the incubation medium and was substantially stimulated by the electron-transport chain activity and valinomycin. At neutral pH of the incubation medium, the rate of K+-dependent swelling of mitochondria decreased by 30–50% after adding 1 mM ATP but was insensitive to other nucleotides (GTP, UTP, and CTP). In the medium acidified to pH 6.0, the addition of ATP caused shrinkage of mitochondria that had been swollen in the KCl medium. In the absence of this nucleotide, the kinetics of K+-dependent swelling of mitochondria was considerably decelerated upon the acidification of the incubation medium. The effects of ATP were independent of the presence or absence of oligomycin and atractyloside. However, the ATP-dependent shrinkage of mitochondria was inhibited in the presence of quinine, and this agent also inhibited K+-dependent swelling of organelles in potassium acetate solutions. The presence of K+ ions in the incubation medium caused a rapid dissipation of the mitochondrial membrane potential () that was generated during succinate oxidation. The addition of ATP to the reaction medium resulted in the oligomycin-insensitive restoration of . The results are regarded as evidence that the membrane of taproot mitochondria is endowed with functionally active ATP-sensitive K+ uniporter. This system is likely to represent a K+ channel that catalyzes the electrogenic transfer of potassium ions to the mitochondrial matrix. It is supposed that the membrane of taproot mitochondria also contains a quinine-sensitive K+/H+ antiporter that catalyzes the efflux of potassium from the matrix or, on the contrary, the accumulation of K+ in the presence of potassium acetate.Translated from Fiziologiya Rastenii, Vol. 52, No. 2, 2005, pp. 209–215.Original Russian Text Copyright © 2005 by Shugaev, Andreev, Vyskrebentseva.This revised version was published online in April 2005 with a corrected cover date. 相似文献
11.
Butacaine and certain other local anesthetics markedly stimulate the rate, extent, and efficiency of respiration-dependent contraction of heart mitochondria in nitrate salts at alkaline pH. The local anesthetics also induce respiratory control associated with contraction (i.e., the elevated rate of respiration during contraction declines to a State 4-like controlled rate when contraction is complete) so that the reaction at alkaline pH closely resembles the rapid and highly efficient process seen at neutral pH. Respiration-dependent contraction appears to be an osmotic response to cation extrusion on an endogenous cation/H+ exchanger (G. P. Brierley, M. Jurkowitz, E. Chavez, and D. W. Jung, 1977, J. Biol. Chem.252, 7932–7939). At alkaline pH, net ion extrusion is slow and inefficient due to the elevated permeability of the membrane to monovalent cations through a putative uniport pathway. Butacaine and other local anesthetics seem to decrease influx-efflux cycling of cations at alkaline pH by restricting cation influx through this uniport. Passive swelling at pH 8.3 in nitrate salts indicates that the uniport reaction is sensitive to Ca2+ and has a cation-selectivity of Na+ > K+ > Li+. Butacaine does not inhibit passive swelling under these conditions but produces effects identical to those of classical uncouplers and consistent with increased H+ conductance and accelerated influx of cations by cation/H+ exchange in nonrespiring mitochondria. However, since contraction in respiring mitochondria is inhibited by uncouplers but stimulated by butacaine, it is apparent that butacaine is not an effective proton conductor in energized mitochondria. 相似文献
12.
The swelling of rat liver mitochondria observed after addition of Ca2+, phosphate or valinomycin under suitable experimental conditions is inhibited by 19-nor-ethynyl-testosterone acetate (NEA) in the concentration range from 3 to 60 μm. The inhibition is proportional to NEA concentration and occurs when swelling is supported by oxidation of NAD-linked substrates (malate-glutamate), or endogenous substrate. Little or no inhibition occurs when swelling is supported by succinate oxidation. These observations suggest a site-specific effect near the NADH-flavoprotein portion of the respiratory chain. NEA also inhibits slightly the ATP-dependent contraction of Ca2+ swollen mitochondria, indicating a secondary effect on the energy-transfer mechanism. In contrast to these effects, NEA does not significantly affect: (a) H+ ejection after Ca2+ uptake supported by succinate oxidation; (b) valinomycin-induced swelling supported by ATP addition; (c) Na-acetate-induced swelling, in which the permeability of membranes to Na+ is rate limiting; and (d) loss of endogenous mitochondrial pyridine nucleotide. Other steroids such as androsterone, 17β-estradiol, and testosterone derivatives affect mitochondrial swelling like NEA, though to a lesser extent. Effects (a) and (d) are at variance with a previously postulated increase of mitochondrial permeability by steroids, accompanied by swelling. The studies which led to this postulate were carried out at steroid concentrations above 200 μm, where nonspecific effects on membrane permeability may well occur. 相似文献
13.
1. Addition of succinate to valinomycin-treated mitochondria incubated in KCl causes a large electrolyte penetration. The process depends on a steady supply of energy and involves a continuous net extrusion of protons. Rates of respiration and of electrolyte penetration proceed in a parallel manner.2. A passive penetration of K+ salt of permeant anions occurs in respiratory-inhibited mitochondria after addition of valinomycin. Addition of succinate at the end of the passive swelling starts an active extrusion of anions and cations with restoration of the initial volume. The shrinkage is accompanied by a slow reuptake of protons. The initiation of the active shrinkage correlates with the degree of stretching of the inner membrane. The extrusion of electrolytes is inhibited by nigericin, while it is only slightly sensitive to variations of the valinomycin concentration larger than two orders of magnitude.3. Passive swelling and active shrinkage occurs also when K+ is replaced by a large variety of organic cations. The rate of organic cation penetration is enhanced by tetraphenylboron, while the rate of electrolyte extrusion is insensitive to variation of the tetraphenylboron concentration.4. Active shrinkage, either with K+ or organic cation salts, is inhibited by weak acids. The phosphate inhibition is removed by SH inhibitors. The active shrinkage is also inhibited by mersalyl to an extent of about 60%.5. Three models of active shrinkage are discussed: (a) mechanoprotein, (b) electrogenic proton pump, and (c) proton-driven cation anion pump. 相似文献
14.
Dennis W. Jung Tahira Farooqui Eric Utz Gerald P. Brierley 《Journal of bioenergetics and biomembranes》1984,16(5-6):379-390
Quinine inhibits the respiration-dependent extrusion of K+ from Mg2+-depleted heart mitochondria and the passive osmotic swelling of these mitochondria in K+ and Na+ acetate at alkaline pH. These observations concur with those of Nakashima and Garlid (J. Biol. Chem.
257, 9252, 1982) using rat liver mitochondria. Quinine also inhibits the respiration-dependent contraction of heart mitochondria swollen passively in Na+ or K+ nitrate and the increment of elevated respiration associated with the extrusion of ions from these mitochondria. Quinine, at concentrations up to 0.5 mM, inhibits the respiration-dependent42K+/K+ exchange seen in the presence of mersalyl, but higher levels of the drug produce increased membrane permeability and net K+ loss from the matrix. These results are all consistent with an inhibition of the putative mitochondrial K+/H+ antiport by quinine. However, quinine has other effects on the mitochondrial membrane, and possible alternatives to this interpretation are discussed. 相似文献
15.
Ariel R. Cardoso João Victor Cabral-Costa Alicia J. Kowaltowski 《Journal of bioenergetics and biomembranes》2010,42(3):245-253
High fat diets are extensively associated with health complications within the spectrum of the metabolic syndrome. Some of
the most prevalent of these pathologies, often observed early in the development of high-fat dietary complications, are non-alcoholic
fatty liver diseases. Mitochondrial bioenergetics and redox state changes are also widely associated with alterations within
the metabolic syndrome. We investigated the mitochondrial effects of a high fat diet leading to non-alcoholic fatty liver
disease in mice. We found that the diet does not substantially alter respiratory rates, ADP/O ratios or membrane potentials
of isolated liver mitochondria. However, H2O2 release using different substrates and ATP-sensitive K+ transport activities are increased in mitochondria from animals on high fat diets. The increase in H2O2 release rates was observed with different respiratory substrates and was not altered by modulators of mitochondrial ATP-sensitive
K+ channels, indicating it was not related to an observed increase in K+ transport. Altogether, we demonstrate that mitochondria from animals with diet-induced steatosis do not present significant
bioenergetic changes, but display altered ion transport and increased oxidant generation. This is the first evidence, to our
knowledge, that ATP-sensitive K+ transport in mitochondria can be modulated by diet. 相似文献
16.
Corn mitochondria in 100 millimolar KCl show accelerated passive swelling upon addition of uncoupler. This unusual response has been compared with swelling produced by valinomycin, tripropyltin, and nigericin. It is concluded that the driving force for swelling lies with the chloride gradient and a high PCl:PK ratio, the chloride influx creating a negative membrane potential. The action of uncoupler is to facilitate K+ influx via the endogenous H+/K+ antiporter. The antiporter is active over the pH range 6 to 8, is not sensitive to Mg2+ concentration, and is not inactivated by aging. It is not clear why corn mitochondria show this exceptional activity of the H+/K+ antiporter in K+ influx. It is speculated that during isolation the antiporter may be exposed or activated, and that it contributes to cyclic K+ transport and high State 4 respiration rates. 相似文献
17.
I. V. Shemarova K. V. Sobol’ S. M. Korotkov V. P. Nesterov 《Journal of Evolutionary Biochemistry and Physiology》2014,50(3):221-226
Inoptopic effect of yttrium acetate (Y3+) on myocardium of the marsh frog Rana ridibunda and its effect on ion transport across the inner mitochondrial membrane (IMM) of rat heart was studied. Y3+ was found to decrease the rate of heart contractions and to stimulate ion transport in the rat heart mitochondria in media with 10 mM glutamate and 2 mM malate. Presence of Y3+ induced inhibition of energy-dependent Ca2+ transport into mitochondria, which was expressed as a marked decrease of their swelling in the media containing 125 mM NH4NO3 and Ca2+ or 25 mM potassium acetate, 100 mM sucrose and Ca2+. It is suggested that the Y3+-induced decrease in rat muscle contractions is determined not only by direct suppressing effect of Y3+ on potential-modulated Ca2+-channels of pacemaker and contractile cardiomyocytes (CM), but also by its indirect effect on Ca2+-carrier in IMM. The data confirming that Y3+ activates energy-dependent K+ transport catalyzed by mitochondrial uniporter and blocks Ca2+-channels in the mitochondrial membrane are important for more complete understanding of mechanisms of the Y3+ action on vertebrates and human CM. 相似文献
18.
Nonesterified long-chain fatty acids (myristic, palmitic, oleic and arachidonic), added at low amounts (around 20 nmol/mg protein) to rat liver mitochondria, energized by respiratory substrates and suspended in isotonic solutions of KCl, NaCl, RbCl or CsCl, adjusted to pH 8.0, induce a large-scale swelling followed by a spontaneous contraction. Such swelling does not occur in alkaline solutions of choline chloride or potassium gluconate or sucrose. These changes in the matrix volume reflect a net uptake, followed by net extrusion, of KCl (or another alkali metal chloride) and are characterized by the following features: (1) Lowering of medium pH from 8.0 to 7.2 results in a disappearance of the swelling-contraction reaction. (2) The contraction phase disappears when the respiration is blocked by antimycin A. (3) Quinine, an inhibitor of the K+/H+ antiporter, does not affect swelling but suppresses the contraction phase. (4) The swelling phase is accompanied by a decrease of the transmembrane potential and an increase of respiration, whereas the contraction is followed by an increase of the membrane potential and a decrease of oxygen uptake. (5) Nigericin, a catalyst of the K+/H+ exchange, prevents or partly reverses the swelling and partly restores the depressed membrane potential. These results indicate that long-chain fatty acids activate in liver mitochondria suspended in alkaline saline media the uniporter of monovalent alkali metal cations, the K+/H+ antiporter and the inner membrane anion channel. These effects are presumably related to depletion of mitochondrial Mg2+, as reported previously [Arch. Biochem. Biophys. 403 (2002) 16], and are responsible for the energy-dissipating K+ cycling. The uniporter and the K+/H+ antiporter are in different ways activated by membrane stretching and/or unfolding, resulting in swelling followed by contraction. 相似文献
19.
Mitochondria isolated from potato tubers fail to undergo passive osmotic swelling when suspended in isotonic Na+ acetate or phosphate, in NaCl following addition of tripropyltin, or in Na+ nitrate following addition of an uncoupler. Swelling under each of these conditions in mitochondria from other sources has been attributed to the inward movement of Na+ on an endogenous Na+/H+ exchanger. Such a monovalent cation/H+ exchanger has also been implicated in respiration-dependent cation extrusion and contraction of swollen mitochondria. Potato mitochondria swollen in chloride and nitrate salts extrude ions and contract when respiration is initiated. The contraction reaction is slower and less efficient than that in beef heart mitochondria, but like the latter, is sensitive to uncouplers and stimulated by nigericin, butacaine, and Mg2+. These comparative studies suggest that a cation+/H+ exchanger is present in potato tuber mitochondria, but that it functions exclusively as a cation-extruding mechanism. They further suggest that cation+/H+ exchange activity is not identical in mitochondria from different sources and that these exchange components may have a directionality and regulatory features which differ with the metabolic needs of the source tissue. 相似文献
20.
Synaptosomes swell rapidly in isosmotic solutions of glycerol or urea, but the swelling in solutions of larger non-electrolytes, such as erythritol, glucose or sucrose is slower. The permeability of synaptosomes to non-electrolytes is temperature dependent, and the low activation energies for the permeation of urea (13 kcal/mol) and erythritol (9.5 kcal/mol) indicate that the penetration of non-electrolytes into the synaptosomes does not imply complete dehydration of the molecules. The relative permeability of synaptosomes to cations, as measured by the rate of swelling in isosmotic solutions of acetate salts is in the order: NH+4 > Na+ > Li+ > K+ > Ca2+. The ionophores, X-537A and nigericin, or valinomycin + FCCP, which promote exchange of cations for H+, cause swelling of synaptosomes in solutions of potassium salts of acetate or propionate, but not in KCI, whereas H+ release is higher in KCI medium. This suggests that the organic unions cross the membrane after combining with H+ to form the respective weak acids. The relative permeability to anions is in the order: acetate ? propionate > Cl? > SO2-4? maleate ? succinate. The energies of activation for the permeability of synaptosomes to potassium acetate in the presence of X-537A or gramicidin D are 13 kcal/mol and 7.5 kcal/mol, respectively, which reflects different mechanisms of action for the two ionophores in the membranes. 相似文献