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The right end of transposable bacteriophage D108 contains a 520 base pair protein-encoding sequence not present in bacteriophage Mu. 总被引:1,自引:0,他引:1
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We have cloned and characterized the right end terminal 796 bp of the transposable Mu-like bacteriophage D108. This region encompasses a 520 bp region of D108-specific sequences not present in phage Mu that contain an open reading frame encoding a 12 KDa protein. This protein can be visualized in vivo when the region is placed downstream from the strong lac UV5 promoter. The open reading frame can be expressed from the dam-regulated mod promoter (for modification of D108 DNA), yet also contains its own dam-independent promoter for expression that is detectable by northern blot analysis late in the D108 lytic cycle. Comparison of this region of D108 DNA with the corresponding region of Mu DNA suggests that a complex rearrangement has occurred at the phages' right ends during their evolution. 相似文献
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Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20 总被引:18,自引:0,他引:18
G Fayat J F Mayaux C Sacerdot M Fromant M Springer M Grunberg-Manago S Blanquet 《Journal of molecular biology》1983,171(3):239-261
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Nucleotide sequence of the immunity region of bacteriophage Mu 总被引:22,自引:0,他引:22
Harro Priess Dietmar Kamp Regine Kahmann Bärbel Bräuer Hajo Delius 《Molecular & general genetics : MGG》1982,186(3):315-321