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1.
U. Theodore Hammer 《International Review of Hydrobiology》1994,79(2):235-248
Five closed saline lakes near Humboldt, Saskatchewan, were found to be meromictic. Two of these lakes (Waldsea, Deadmoose) were first discovered to be meromictic in the early 1970s and three (Arthur, Marie, Sayer) in 1985. The origin of their meromixis is ectogenic. One of the lakes, Waldsea, had surface salinities far higher in 1960–1961 than those of 1970 or later and as high as that of the monimolimnion occasionally was from 1970 to the present. During the late 1960s to 1980 the lake level of Waldsea rose four metres as a result of higher than normal snowpacks and subsequent high snowmelt runoff. Endogenic processes of freezing out of salts from the upper metre during ice formation and precipitation of sodium sulphate during autumn cooling also promote meromixis. The lakes which are located in depressions in a relatively flat topography are very exposed to periodic high velocity westerly winds. Although Deadmoose and Waldsea lakes are relatively deep, Arthur, Marie and Sayer lakes have maximum depths of only three to five metres. Meromixis has persisted until the present in three lakes but Marie and Arthur lakes became holomictic during the autumn of 1988, a severe drought year. Bacterial plates were prominent in Waldsea, Deadmoose and Sayer lakes. BChl-a and BChl-d were present in 1988 with maxima of 2652 mg · m−3 BChl-a and 4290 mg · m−3 BChl-d in Sayer Lake. BChl-a virtually disappeared in subsequent years. 相似文献
2.
Temporal Patterns in Bacterial Communities in Three Temperate Lakes of Different Trophic Status 总被引:4,自引:0,他引:4
Despite considerable attention in recent years, the composition and dynamics of lake bacterial communities over annual time scales are poorly understood. This study used automated ribosomal intergenic spacer analysis (ARISA) to explore the patterns of change in lake bacterial communities in three temperate lakes over 2 consecutive years. The study lakes included a humic lake, an oligotrophic lake, and a eutrophic lake, and the epilimnetic bacterial communities were sampled every 2 weeks. The patterns of change in bacterial communities indicated that seasonal forces were important in structuring the behavior of the bacterial communities in each lake. All three lakes had relatively stable community composition in spring and fall, but summer changes were dramatic. Summertime variability was often characterized by recurrent drops in bacterial diversity. Specific ARISA fragments derived from these lakes were not constant among lakes or from year to year, and those fragments that did recur in lakes in different years did not exhibit the same seasonal pattern of recurrence. Nonetheless, seasonal patterns observed in 2000 were fairly successful predictors of the rate of change in bacterial communities and in the degree of autocorrelation of bacterial communities in 2001. Thus, seasonal forces may be important structuring elements of these systems as a whole even if they are uncoupled from the dynamics of the individual system components. 相似文献
3.
4.
An extension of Walker and Likens' (1974). scheme for the classification of meromictic lakes of the world has been based in part upon the mutual incompatibility of certain common chemical species and their generation within the monimolimnia of biogenic meromictic lakes. 相似文献
5.
Brian V. Timms U. Theodore Hammer John W. Sheard 《International Review of Hydrobiology》1986,71(6):759-777
The spring benthos of 22 lakes ranging from 1–88 gl−1 salinity contained 58 species of macroinvertebrates, but only 23 species occurred in waters >3 gl−1. The amphipod Hyalella azteca and the chironomids Procladius freemani, Chironomus nr. muratensis and Cryptochironomus spp. were important at lower salinities (1–12 gl−1) whereas the chironomids Tanypus nubifer, Cricotopus ornatus and Chironomus nr. annularis dominated at moderate salinities (5–30 gl−1) and dolichopodid and ephyrid dipterans were the only species in hypersaline lakes (>50 gl−1). Diversity decreased significantly with increased salinity. Mean dry biomass ranged from 0–9.12 gm−2, showing little correlation with salinity, though hyposaline lakes often had elevated values and hypersaline lakes very low values. Shallow lakes (<5 m) had significantly lower standing crops. There were long term changes in biomass (over 45 years) in some lakes due to cultural eutrophication or secular changes in salinity. Chironomids were by far the dominant contributors to biomass at salinities to 50 gl−1, above which dolichopodid and ephyrid dipterans dominated. The lakes were classified into four groups—subsaline, hyposaline, shallow hypo-mesosaline and hypersaline, reflecting the importance of salinity and also relative depth as major controlling factors. 相似文献
6.
Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (δ13C) and hydrogen sulfide and sulfate (δ34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.__________Translated from Mikrobiologiya, Vol. 74, No. 4, 2005, pp. 552–561.Original Russian Text Copyright © 2005 by Savvichev, Rusanov, Rogozin, Zakharova, Lunina, Bryantseva, Yusupov, Pimenov, Degermendzhi, Ivanov. 相似文献
7.
U. Theodore Hammer 《International Review of Hydrobiology》1978,63(2):173-177
The historical background and utilization of southern Saskatchewan is described. The utilization of the saline lakes is discussed and the rationale for studying them is outlined. 相似文献
8.
M. Coci G. W. Nicol G. N. Pilloni M. Schmid M. P. Kamst-van Agterveld P. L. E. Bodelier H. J. Laanbroek 《Applied and environmental microbiology》2010,76(6):1813-1821
In addition to the benthic and pelagic habitats, the epiphytic compartment of submerged macrophytes in shallow freshwater lakes offers a niche to bacterial ammonia-oxidizing communities. However, the diversity, numbers, and activity of epiphytic ammonia-oxidizing bacteria have long been overlooked. In the present study, we analyzed quantitatively the epiphytic communities of three shallow lakes by a potential nitrification assay and by quantitative PCR of 16S rRNA genes. On the basis of the m2 of the lake surface, the gene copy numbers of epiphytic ammonia oxidizers were not significantly different from those in the benthic and pelagic compartments. The potential ammonia-oxidizing activities measured in the epiphytic compartment were also not significantly different from the activities determined in the benthic compartment. No potential ammonia-oxidizing activities were observed in the pelagic compartment. No activity was detected in the epiphyton of Chara aspera, the dominant submerged macrophyte in Lake Nuldernauw in The Netherlands. The presence of ammonia-oxidizing bacterial cells in the epiphyton of Potamogeton pectinatus was also demonstrated by fluorescent in situ hybridization microscopy images. By comparing the community composition as assessed by the 16S rRNA gene PCR-denaturing gradient gel electrophoresis approach, it was concluded that the epiphytic ammonia-oxidizing communities consisted of cells that were also present in the benthic and pelagic compartments. Of the environmental parameters examined, only the water retention time, the Kjeldahl nitrogen content, and the total phosphorus content correlated with potential ammonia-oxidizing activities. None of these parameters correlated with the numbers of gene copies related to ammonia-oxidizing betaproteobacteria.In ammonium-rich environments such as eutrophic lakes, ammonia-oxidizing Betaproteobacteria (β-AOB) perform the first, often rate-limiting step in the process of nitrification, hence playing an important role in the nitrogen turnover in a wide range of natural and artificial habitats (31). Their monophyletic nature allowed the successful application of molecular techniques based on the genes coding for the 16S rRNA gene and the A subunit of the ammonia monooxygenase enzyme (amoA). β-AOB have been considered an ideal model group in molecular microbial ecology (31). The last few decades have seen significantly increased numbers of studies focusing on diversity (7, 22, 24, 44, 48, 54) and niche differentiation and related driving factors (8, 13, 14, 30, 33, 45), as well as on the abilities of ammonia-oxidizing bacteria to cope with contaminants (40, 49, 53), environmental stresses (18, 34, 39), and global change (23, 43). The discovery of the process of anaerobic ammonia oxidization (42), together with the isolation of members of the kingdom Crenarchaeota able to oxidize ammonia (26), is currently changing and deepening the knowledge and understanding of the microorganisms involved in the nitrogen cycle.Nitrification in freshwater as well as in shallow marine lagoon systems has been assumed to be associated with the sediment rather than with the overlying water (2). However, when planktonic nitrification rates are integrated over the whole water column, Vincent and Downes (59) demonstrated the impact of the pelagic community on the total nitrification process in lakes. In shallow freshwater lakes populated by large stands of macrophytes, the role of epiphytic nitrification must also be taken into account, since submerged macrophytes can provide a large accessible surface area for attached microorganisms (61). The bacterial ammonia oxidizers inhabiting the epiphytic compartment have been the subject of a limited number of studies. Eriksson and colleagues (15-17) measured the nitrification rates on the leaves and litter of submerged macrophytes, and Körner (29) reported that considerable numbers of ammonia-oxidizing bacteria colonize the leaves of different species of submerged macrophytes by means of most-probable-numbers counts. In a recent study of the β-AOB in shallow freshwater lakes (12), we described in a qualitative way that the epiphytic communities are composed of members of the Nitrosomonas oligotropha lineage and cluster 3 of the Nitrosospira lineage (48).The present study focused on a more quantitative estimation of the ammonia-oxidizing bacteria in the epiphyton of two different submerged macrophytes, i.e., Potamogeton pectinatus and Chara aspera, present in three shallow freshwater lakes, which form part of the series of lakes studied before (12). We hypothesized that the numbers of cells would differ between the epiphytic, benthic, and pelagic compartments, as previously observed for the community composition, due to the prevailing environmental conditions (12). 相似文献
9.
Lamprocystis roseopersicina was found to be the dominant phototrophic bacterium in meromictic Deadmoose Lake. Using carbon-14 it was determined that phototrophic bacteria fixed 14.3 g C m−2 year−1, 17.1 % of the total limnetic primary production. Experiments indicated low light intensities (X = 8.87 × 10−2 cal cm−2hr−1) to be the most important factor limiting bacterial production. Lamprocystis was shown to be capable of photoorganoheterotrophic uptake of glucose. 相似文献
10.
Bacterial Communities in Central European Bumblebees: Low Diversity and High Specificity 总被引:1,自引:0,他引:1
Recent studies on the microbial flora of the honeybee gut have revealed an apparently highly specific community of resident
bacteria that might play a role in immune defence and food preservation for their hosts. However, at present, very little
is known about the diversity and ecology of bacteria occurring in non-domesticated bees like bumblebees, which are of similar
importance as honeybees for the pollination of agricultural and wild flowers. To fill this gap in knowledge, we examined six
of the most common bumblebee species in Central Europe from three locations in Germany and Switzerland for their bacterial
communities. We used a culture-independent molecular approach based on sequencing the 16S rRNA gene from a selection of individuals
and examining a larger number of samples by terminal restriction fragment length polymorphism profiles. The gut flora was
dominated by very few and mostly undescribed groups of bacteria belonging to the Proteobacteria, Bacteroidetes, Firmicutes
and Actinobacteria. This core set of bacteria was present in all of the examined bumblebee species. These bacteria are similar
to, but distinct from, bacteria previously described from the honeybee gut. Significant differences were observed between
the communities of bacteria in the different bumblebee species; the effect of sampling location was less strong. A novel group
of Betaproteobacteria additionally shows evidence for host species-specific genotypes. The gut flora of bumblebees therefore
is apparently composed of relatively few highly specialized bacteria, indicating a strong interaction and possibly important
functions with their hosts. 相似文献
11.
Deep-sea hydrothermal vents are considered to be one of the most spectacular ecosystems on Earth. Microorganisms form the basis of the food chain in vents controlling the vent communities. However, the diversity of bacterial communities in deep-sea hydrothermal vents from different oceans remains largely unknown. In this study, the pyrosequencing of 16S rRNA gene was used to characterize the bacterial communities of the venting sulfide, seawater, and tubeworm trophosome from East Pacific Rise, South Atlantic Ridge, and Southwest Indian Ridge, respectively. A total of 23,767 operational taxonomic units (OTUs) were assigned into 42 different phyla. Although Proteobacteria, Actinobacteria, and Bacteroidetes were the predominant phyla in all vents, differences of bacterial diversity were observed among different vents from three oceanic regions. The sulfides of East Pacific Rise possessed the most diverse bacterial communities. The bacterial diversities of venting seawater were much lower than those of vent sulfides. The symbiotic bacteria of tubeworm Ridgeia piscesae were included in the bacterial community of vent sulfides, suggesting their significant ecological functions as the primary producers in the deep-sea hydrothermal vent ecosystems. Therefore, our study presented a comprehensive view of bacterial communities in deep-sea hydrothermal vents from different oceans. 相似文献
12.
A. M. Kropinski 《Applied microbiology》1975,29(4):448-450
By storage in 1% NaCl, genetically characterized strains of Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa were stable for more than 1 year at 4 C. This method of preservation was more effective than maintenance of the strains in stab culture. 相似文献
13.
Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France 总被引:3,自引:3,他引:0
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Anne-C. Lehours Corinne Bardot Aurelie Thenot Didier Debroas Gerard Fonty 《Applied microbiology》2005,71(11):7389-7400
The Bacteria and Archaea from the meromictic Lake Pavin were analyzed in samples collected along a vertical profile in the anoxic monimolimnion and were compared to those in samples from the oxic mixolimnion. Nine targeted 16S rRNA oligonucleotide probes were used to assess the distribution of Bacteria and Archaea and to investigate the in situ occurrence of sulfate-reducing bacteria and methane-producing Archaea involved in the terminal steps of the anaerobic degradation of organic material. The diversity of the complex microbial communities was assessed from the 16S rRNA polymorphisms present in terminal restriction fragment (TRF) depth patterns. The densities of the microbial community increased in the anoxic layer, and Archaea detected with probe ARCH915 represented the largest microbial group in the water column, with a mean Archaea/Eubacteria ratio of 1.5. Terminal restriction fragment length polymorphism (TRFLP) analysis revealed an elevated archaeal and bacterial phylotype richness in anoxic bottom-water samples. The structure of the Archaea community remained rather homogeneous, while TRFLP patterns for the eubacterial community revealed a heterogeneous distribution of eubacterial TRFs. 相似文献
14.
15.
Central Asia is one of the world largest regions, which has been relatively poorly studied from the microbiological point of view. The lakes of this region have a broad range of ecological conditions, from lowmineral to hypersaline ones. Long-term observations revealed that microbial communities of these lakes reflect the climatic and ecological changes in this region. Genomic research showed that some microbial species were found only in Central Asian lakes, but not in other regions of the world. The combination of these factors makes Central Asian lakes a promising subject for microbiological investigation. 相似文献
16.
Meromictic Lake Kivu is renowned for its enormous quantity of methane dissolved in the hypolimnion. The methane is primarily of biological origin, and its concentration has been increasing in the past half-century. Insight into the origin of methane production in Lake Kivu has become relevant with the recent commercial extraction of methane from the hypolimnion. This study provides the first culture-independent approach to identifying the archaeal communities present in Lake Kivu sediments at the sediment-water interface. Terminal restriction fragment length polymorphism analysis suggests considerable heterogeneity in the archaeal community composition at varying sample locations. This diversity reflects changes in the geochemical conditions in the sediment and the overlying water, which are an effect of local groundwater inflows. A more in-depth look at the archaeal community composition by clone library analysis revealed diverse phylogenies of Euryarchaeota and Crenarachaeota. Many of the sequences in the clone libraries belonged to globally distributed archaeal clades such as the rice cluster V and Lake Dagow sediment environmental clusters. Several of the determined clades were previously thought to be rare among freshwater sediment Archaea (e.g., sequences related to the SAGMEG-1 clade). Surprisingly, there was no observed relation of clones to known hydrogentrophic methanogens and less than 2 % of clones were related to acetoclastic methanogens. The local variability, diversity, and novelty of the archaeal community structure in Lake Kivu should be considered when making assumptions on the biogeochemical functioning of its sediments. 相似文献
17.
Primary production by phytoplankton, efficiency of photosynthesis, and chlorophyll-a concentrations were determined for seven saline lakes that varied widely in ionic concentration and composition. The investigations were done during the summer months of 1972 and 1973. Productivity ranged from 0.001 to 11.135 g C m−3 day−1 and 0.053 to 7.968 g C m−2 day−1. Highest productivities were measured in two lakes that supported blooms of Aphanizomenon flos-aquae and Nodularia spumigena, respectively. Species of Cyanophyceae, Bacillariophyceae and Chlorophyceae dominated the phytoplankton of the study lakes. Active chlorophyll-a ranged from 0.01 to 116 mg m−3. Integral photosynthetic efficiency estimates were <1% except during phytoplankton blooms when they were considerably higher. The overall range of 0.03 to 3.8% is concordant with estimates for other lacustrine ecosystems. The extinction of light caused by photo-synthetic processes, or in situ efficiency, was <1% in the trophogenic zone for most lakes but, it was considerably higher during blooms. In situ efficiencies invariably increased with depth in ail lakes. 相似文献
18.
细根是植物吸收水分和养分的主要器官, 细根生物量对盐土地人工绿化植被生态修复具有重要意义。以3种人工林为研究对象, 分别对其细根生物量、垂直分布及各形态指标的变化特征进行分析。结果表明, 响叶杨(Populus adenopoda)林、普陀樟(Cinnamomum japonicum)林和落羽杉(Taxodium distichum)林0-40 cm土层的平均细根生物量分别为1 699.75、498.50和520.06 g·m-2。3种林分在0-10 cm土层中的细根生物量占整个细根生物量的50%以上, 随着土层的增加细根生物量呈现指数减少(P<0.05)。在生长季节内细根生物量呈双峰变化, 不同月份间存在显著差异。活细根生物量和比根长均表现为普陀樟林<落羽杉林<响叶杨林。将细根各项指标与3种环境因子进行相关分析, 发现土壤含水量与活细根生物量及根长密度呈显著正相关(P<0.01)。CCA分析表明, 土壤含盐量是影响活细根各项指标垂直变化的主要限制因子, 而高盐可能对细根生物量及分布有不利影响。 相似文献
19.
根据Amsel标准及Nugent标准,确诊筛选健康妇女及细菌性阴道病(bacterial vaginosis,BV)患者各3例.提取其阴道分泌物样本的总DNA,构建16S rRNA基因克隆文库,并对阳性克隆进行ARDRA和测序分析.结果表明,健康妇女样本的基因文库中,分别以卷曲乳酸杆菌(L.crispatus)和惰性乳酸杆菌(L. iners)的克隆子占较大比例,另外存在少量的阴道乳酸杆菌(L.vaginalis)或詹氏乳酸杆菌(L.jensenii)的克隆子.BV患者样本的基因文库中,克隆子所代表的菌种类型明显增多,但均以阴道加德纳氏菌(Gardnerella vaginalis)和阴道阿托波氏菌(Atopobium vaginae)的克隆子占较大比例,且无乳酸杆菌克隆子.说明健康妇女阴道菌群的种类单一,以乳酸杆菌占优势,L. iners为优势菌种之一;BV患者阴道菌群的种类复杂多样,但均以Gardnerella vaginalis及Atopobium vaginae共同占优势. 相似文献
20.
根据Amsel标准及Nugent标准, 确诊筛选健康妇女及细菌性阴道病(bacterial vaginosis, BV)患者各3例。提取其阴道分泌物样本的总DNA, 构建16S rRNA基因克隆文库, 并对阳性克隆进行ARDRA和测序分析。结果表明, 健康妇女样本的基因文库中, 分别以卷曲乳酸杆菌(L. crispatus)和惰性乳酸杆菌(L. iners)的克隆子占较大比例, 另外存在少量的阴道乳酸杆菌(L. vaginalis)或詹氏乳酸杆菌(L. jensenii)的克隆子。BV患者样本的基因文库中, 克隆子所代表的菌种类型明显增多, 但均以阴道加德纳氏菌(Gardnerella vaginalis)和阴道阿托波氏菌(Atopobium vaginae)的克隆子占较大比例, 且无乳酸杆菌克隆子。说明健康妇女阴道菌群的种类单一, 以乳酸杆菌占优势, L. iners为优势菌种之一; BV患者阴道菌群的种类复杂多样, 但均以Gardnerella vaginalis及Atopobium vaginae共同占优势。 相似文献