老年人胃内菌群研究

目的研究人体胃内菌群,探讨老年与非老年人胃内菌群的差异。方法选择67例无严重胃肠道疾病的患者为研究对象。其中男性50例、女性17例。年龄≥60岁53例,〈60岁14例。胃镜下取胃组织及胃液,测胃液pH,并做胃组织需氧、厌氧细菌培养及真菌培养,计数胃组织细菌培养数量。16SrRNA法鉴定胃组织细菌种类。真菌的鉴定按微生物科常规菌种方法鉴定。结果老年人中胃内需氧细菌培养阳性为23例（48.93%）,12例（25.53%）胃内需氧菌培养〉1×10^5CFU/g;厌氧细菌培养阳性为22例（46.81%）,12例（25.53%）胃内厌氧菌培养〉1×10^5CFU/g。非老年人中需氧细菌培养阳性为4例（28.57%）,1例（7.14%）胃内需氧菌培养〉1×10^5CFU/g;厌氧细菌培养阳性为4例（28.57%）,1例（7.14%）胃内厌氧菌培养〉1×10^5CFU/g。但老年人与非老年人比较,细菌培养阳性率及细菌培养〉1×10^5CFU/g的比率差异无显著性。仅1例老年人胃组织分离出真菌,为白色念珠菌。胃内共分离出细菌69株,其中革兰阳性球菌31株（44.93%）,革兰阳性杆菌12株（17.39%）,革兰阴性球菌11株（15.94%）,革兰阴性杆菌15株（21.74%）。需氧菌13株（18.84%）,需氧兼性厌氧菌54株（78.26%）,专性厌氧菌2株（2.90%）。老年人胃内常见的细菌是：链球菌、大肠埃希菌、奈瑟菌;非老年人胃内常见的细菌是：链球菌和大肠埃希菌。多为口咽部和胃肠道常见菌群,部分为条件致病菌。结论约46%～48%的老年人胃内细菌培养阳性,约25%的老年人有胃内细菌过度生长（〉1×10^5CFU/g）。约28%的非老年人胃内细菌培养阳性,约7%的非老年人有胃内细菌过度生长。老年人胃内菌群分布与非老年人相似,为口咽部和胃肠道常见菌群,部分为条件致病菌。     

Immunohistochemical study of the expression of trophoblastic beta-1-globulin in the gastric epithelium of man in stomach cancer]

The expression of specific-pregnancy beta 1-globulin (SP1) was studied in gastric mucosa with chronic gastritis. The expression of SP1 was revealed in focus of intestinal metaplasia, dysplasia as well as in cover epithelial cells.     

Validation of the stomach-flushing technique for obtaining stomach contents of Penguins

The efficiency of the stomach flushing technique in obtaining complete stomach contents was tested on Little, Gentoo and Rockhopper Penguins. This technique was validated by feeding the penguins known amounts of fish and subsequently flushing their stomachs after specified time intervals. Examination of the contents showed that the method is effective and offers an alternative to killing penguins in order to obtain stomach contents. The effects of different states of stomach fullness on food recovery rates highlighted the necessity for multiple flushing. Quantitative information on the effect of time between feeding and stomach flushing on the recovery rates was also obtained. Where stomach contents were relatively undigested the rate of retrieval of fish was 90–100%, but this rate decreased with time and in no cases in which stomach contents were in advanced stage of digestion was the retrieval rate higher than 80^o. Inclusion of the Gentoo and Rockhopper Penguins in the validation trials showed that the size of the penguin does not affect recovery rate.     

Morphological research on the effect of vagotomy on the parietal microflora of the stomach and duodenum

The effect of vagotomy on parietal microflora was studied in intact rats and rats with chronic experimental gastric ulcer using stereometry, as well as light, transmission and scanning electron microscopy. Vagotomy was found to increase the relative amount of parietal microflora on day 30 following denervation, especially in the duodenum and pylorus. Chronic gastric ulcers are also associated with a rise in the relative amount of parietal microflora.     

Quantitative evaluation of the point method for fish stomach contents analysis

To test the accuracy of the point methods a simulation was conducted using simulated stomachs. Results from seven participants told to analyse the stomachs by the point and percentage methods differed considerably among subjects and from the control.     

In silico analysis of stomach lineage specific gene set expression pattern in gastric cancer

Stomach lineage specific gene products act as a protective barrier in the normal stomach and their expression maintains the normal physiological processes, cellular integrity and morphology of the gastric wall. However, the regulation of stomach lineage specific genes in gastric cancer (GC) is far less clear. In the present study, we sought to investigate the role and regulation of stomach lineage specific gene set (SLSGS) in GC. SLSGS was identified by comparing the mRNA expression profiles of normal stomach tissue with other organ tissue. The obtained SLSGS was found to be under expressed in gastric tumors. Functional annotation analysis revealed that the SLSGS was enriched for digestive function and gastric epithelial maintenance. Employing a single sample prediction method across GC mRNA expression profiles identified the under expression of SLSGS in proliferative type and invasive type gastric tumors compared to the metabolic type gastric tumors. Integrative pathway activation prediction analysis revealed a close association between estrogen-α signaling and SLSGS expression pattern in GC. Elevated expression of SLSGS in GC is associated with an overall increase in the survival of GC patients. In conclusion, our results highlight that estrogen mediated regulation of SLSGS in gastric tumor is a molecular predictor of metabolic type GC and prognostic factor in GC.     

DNA-based identification of larval cod in stomach contents of predatory fishes

Predator-prey interactions play an influential role in determining the demographics of a population or species. In the Northwest Atlantic, Atlantic cod, Gadus morhua, once the basis of a lucrative commercial fishery, have not recovered despite regulations imposed on the fishery to reduce harvest rates. One possible reason for the lack of recovery is that high predation pressure on juvenile and larval stages, particularly from species such as herring and mackerel, may regulate the abundance of cod. However, traditional methods used to identify larval cod and haddock often fail when applied to partially digested remains. Here, we described a DNA-based assay to identify the presence of digested cod remains from the stomachs of predatory fish species. After development, the assay was tested on two sets of field samples. Larval and juvenile cod were successfully detected in both tests.