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1.
Flight activities of three Spodoptera species were measured by the aid of flight actograph: S. litura and S. exuiga being regarded as long‐distance migratory insects, and S. depravata being non‐migratory and diapause‐inducible species. In all species tested, flight activities were observed only in scotophase, males showed far higher activities than females, being several times higher at the time of maximum flight activity, which was observed within 2 days after adult eclosion. Total flight activity in males was highest in S. litura, some being flyable even 12 days after eclosion, followed by S. exigua being one‐third compared to the former species, while in S. depravata flight activity was nearly half of that of the second species and most ceased to fly within a week after eclosion. There occurred species‐specific daily rhythms in flight activity during respective scotophase. In S. litura, both females and males exhibited a peak of flight activity shortly after light‐off and exhibited the second flight activity in late scotophase, the females slightly but the males more actively compared to early scotophase. In S. exigua, both sexes did not respond to light‐off, did not show a peak of flight activity in early scotophase, whereas males, but not females prominently increased activity toward the end of scotophase. In S. depravata, both sexes exhibited a peak of flight activity in early scotophase, and the males revived flight activity, being maximum shortly before light‐on, but the females did not show a clear rhythm in flight activity. These features observed in flight activity were discussed in relation with migratory capability.  相似文献   

2.
甜菜夜蛾的饲养方法介绍   总被引:16,自引:0,他引:16  
介绍一种甜菜夜蛾的室内饲养方法 ,采用本方法累代饲养甜菜夜蛾 8代 ,平均蛹重达 1 1 2 .9mg ,平均化蛹率 97% ,平均羽化率为 91 .3% ,室内饲养结果表明 ,该饲养方法可用于室内大量饲养甜菜夜蛾  相似文献   

3.
The fall armyworm, Spodoptera frugiperda, is a species native to the Americas and has spread to many countries in Africa and Asia in recent years. Proactive actions for potential invasion of S. frugiperda to China coordinated by government agencies and agricultural extension systems resulted in timely detection in January 2019 in Yunnan province neighboring onto Myanmar. The extensive monitoring in southern provinces of China since February 2019 resulted in dynamic tracking of S. frugiperda spreading to 13 provincial regions in China within 4 months by May 10, 2019, which is crucial for timely management actions in the fields. The first detections of S. frugiperda (corn strain) in China were confirmed using cytochrome oxidase subunit 1 (CO1) and triosephosphate isomerase (Tpi) genes molecular marker method. In addition to S. frugiperda, larvae of three other noctuid species with similar morphological appearance (S. litura, S. exigua and Mythimna separata) can occur simultaneously and cause similar damage in cornfields in southern China. Thus, we can use both morphological and molecular marker methods to compare larval stages of four noctuid species. Further, we discuss the risk of potential spread of invasive S. frugiperda to other regions and impact on corn production in China.  相似文献   

4.
There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non‐feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for later reproduction in adults). Therefore, trypsin must always be present so that only a minimal protein loss via egestion occurs. Larvae, however, adjust amylase activity to carbohydrate ingestion, and indeed amylase activity is five‐fold higher in fed larvae compared to unfed larvae. Gut lipase activity is low, typical of insects with a high carbohydrate diet. A flat‐sheet preparation of the ventriculus was used to measure the release of enzymes in response to specific nutrients and known brain/gut hormones in S. frugiperda. Sugars greatly increase (>300%) amylase release, but starch has no effect. Proteins and amino acids have little or no effect on trypsin or aminopeptidase release. The control of enzyme release in response to food is likely mediated through neurohormones. Indeed, an allatostatin (Spofr‐AS A5) inhibits amylase and trypsin, and allatotropin (Manse‐ AT) stimulates amylase and trypsin release. Spofr‐AS A5 also inhibits ileum myoactivity and Manse‐AT stimulates myoactivity. The epithelial secretion rate of amylase and trypsin was about 20% of the amount of enzyme present in the ventricular lumen, which, considering the efficient counter‐current recycling of enzymes, suggests that the secretion rate is adequate to replace egested enzymes. © 2009 Wiley Periodicals, Inc.  相似文献   

5.
核型多角体病毒与侧沟茧蜂对斜纹夜蛾幼虫的协同作用   总被引:4,自引:1,他引:4  
研究了斜纹夜蛾幼虫体内的斜纹夜蛾侧沟茧蜂存活率、发育历期、寄主感染病毒时间、病毒浓度之间的关系,并测定了斜纹夜蛾侧沟茧蜂的传毒效率.结果表明,病毒对寄主体内寄生蜂历期无明显影响,寄生在幼虫体内的寄生蜂能在寄主病死前完成发育,存活比例因寄主感染病毒的时间和浓度而异.斜纹夜蛾被寄生后接种病毒(SINPV),距离寄生时间越长,饲毒浓度越低,寄生蜂完成发育的比例越大,但饲毒时间是主要影响因素.从感病幼虫体内发育成的侧沟茧蜂或曾经在感病寄主上产过卵的寄生蜂,以及通过人工方式使产卵器被病毒污染的寄生蜂,均能携带一定数量的病毒.通过产卵活动,侧沟茧蜂成蜂能在寄主幼虫个体间传递病毒.当寄生蜂在感病的寄主幼虫上产卵带毒后,平均可传递病毒给2.14头幼虫;发育于感病幼虫体内的寄生蜂,平均可传递病毒给2.45头幼虫.通过用病毒液浸茧或用混有病毒的蜂蜜饲喂成蜂等方式使产卵器污染病毒的寄生蜂,传毒效率随饲毒浓度增加而提高,平均可传递病毒1.45头和0.94头幼虫  相似文献   

6.
甜菜夜蛾抗药性研究现状   总被引:6,自引:0,他引:6  
刘向阳  朱福兴  张凯 《昆虫知识》2007,44(5):632-636
就有关甜菜夜蛾Spodoptera exigua(Hbner)对常用杀虫剂的抗药性现状作综述。甜菜夜蛾对拟除虫菊酯类、有机磷类、氨基甲酸酯类杀虫剂已产生了较高水平的抗药性,其中山东泰安抗性种群对氯氟氰菊酯的抗性高达2445.5倍;对多杀菌素等生物杀虫剂产生了中低水平的抗性;对昆虫生长调节剂如虫酰肼的敏感性也有所降低,但昆虫生长调节剂依然是比较理想的防治药剂。对交互抗性及抗性治理也作了阐述。  相似文献   

7.
Abstract. 1. Leaves of tomato (cv. Moneymaker) were artificially damaged and offered to Spodoptera larvae at a range of intervals following damage. Grazing levels on these leaves were compared with those on undamaged leaves on the same or different plants.
2. In separate experiments, three leaves in a middle position on the main stem were clipped and after 48 h grazing levels on undamaged leaves above and below those damaged were compared with similar leaves from control plants.
3. Within 8 h, grazing levels on damaged leaves were significantly lower than those on control leaves, and within 24 h, leaves adjacent to damaged ones were similarly affected. These effects persisted for at least 7 days and leaves above and below those damaged were affected. There was up to nine-fold reduction in area consumed.
4. The possible ecological consequences of reduced palatability at these levels are discussed.  相似文献   

8.
9.
灰翅夜蛾属昆虫的性信息素研究概况   总被引:8,自引:0,他引:8  
本文从雌蛾性信息素组分鉴定、雄蛾对性信息素的行为反应和性信息素的应用3个方面,综述了灰翅夜蛾属昆虫性信息的研究进展,并展望了此属昆虫性信息素的研究前景。  相似文献   

10.
Abstract  The flight activity of Spodoptera litura in tethered conditions is evaluated using a computer-mediated flight-mill in the laboratory. The 3–4-day-old moths fly longer and farther than those of other ages. Male and female moths exhibit similar flight activity. Mating status does not influence the flight duration and distance of 2-day-old females. However, these two flight parameters with a 6-day-old mated female is significantly lower than that of unmated ones. The optimum temperature for flight ranged from 16–24°C, whereas the optimum RH ranged from 60%–100%. During 72-h period, the total flight duration and distance of 1-day-old male and female moths were 19.6 h (± 5.8) and 83.3 km (± 28.4), and 24.0 h (± 7.0) and 105.4 km (± 37.4), respectively. These results indicate that S. litura has a great potential to undertake long-distance migratory flights.  相似文献   

11.
根据斜纹夜蛾发育起点温度和有效积温,探讨了斜纹夜蛾发生期预测方法。结果表明,田间斜纹夜蛾发生高峰期与预测的日期基本吻合。  相似文献   

12.
Insects can produce various antimicrobial peptides (AMPs) upon immune stimulation. One class of AMPs are characterized by their high proline content in certain fragments. They are generally called proline-rich antimicrobial peptides (PrAMPs). We previously reported the characterization of Spodoptera litura lebocin-1 (SlLeb-1), a PrAMP proprotein. Preliminary studies with synthetic polypeptides showed that among the four deductive active fragments, the C-terminal fragment SlLeb-1 (124-158) showed strong antibacterial activities. Here, we further characterized the antibacterial and antifungal activities of 124-158 and its four subfragments: 124-155, 124-149, 127-158, and 135-158. Only 124-158 and 127-158 could agglutinate bacteria, while 124-158 and four subfragments all could agglutinate Beauveria bassiana spores. Confocal microscopy showed that fluorescent peptides were located on the microbial surface. Fragment 135-158 lost activity completely against Escherichia coli and Staphylococcus aureus, and partially against Bacillus subtilis. Only 124-149 showed low activity against Serratia marcescens. Negative staining, transmission, and scanning electron microscopy of 124-158 treated bacteria showed different morphologies. Flow cytometry analysis of S. aureus showed that 124-158 and four subfragments changed bacterial subpopulations and caused an increase of DNA content. These results indicate that active fragments of SlLeb-1 may have diverse antimicrobial effects against different microbes. This study may provide an insight into the development of novel antimicrobial agents.  相似文献   

13.
人工饲料成分对甜菜夜蛾核多角体 病毒产量的影响   总被引:5,自引:1,他引:5  
李广宏  陈其津  庞义 《昆虫学报》2000,43(4):356-363
通过采取单因素和正交组合设计的方法,研究了甜菜夜蛾Spodoptera exigua人工饲料中黄豆粉、酵母粉和麦麸3种主要营养成分对甜菜夜蛾核多角体病毒产量的影响,同时获得甜菜夜蛾核多角体病毒高产优化组合方案:酵母粉7 g、黄豆粉14 g和麦麸6 g。此外,还研究了4种无机盐和1种非蛋白氮对甜菜夜蛾核多角体病毒产量的影响。  相似文献   

14.
收集甜菜夜蛾卵的简便方法   总被引:1,自引:0,他引:1  
介绍了一种收集甜菜夜蛾SpodopteraexiguaH櫣bner卵的简单而有效的方法 ,收卵率达 90 %以上  相似文献   

15.
以甜菜夜蛾Spodoptera exigua(Hübner)幼虫为材料建立了cDNA表达文库,经检测文库的初始滴度为1.1×105pfu/mL,重组率为97%,扩增后文库的滴度为5.4×107pfu/mL。用设计的2对引物筛选该文库,得到468 bp的1个片段,分析后证实是几丁质合成酶基因I保守区域的1个片段。该cDNA文库为进一步筛选甜菜夜蛾功能基因奠定了基础。  相似文献   

16.
寄主植物对斜纹夜蛾酯酶和乙酰胆碱酯酶活性的影响   总被引:2,自引:0,他引:2  
利用生物测定与生物化学的方法,就取食不同寄主植物的2个斜纹夜蛾[Spodoptera litura(Fab.)]品系对丙溴磷和灭多威的敏感性及其体内的酯酶与乙酰胆碱酯酶的活性变化进行了研究。结果表明,取食不同寄主植物的斜纹夜蛾对丙溴磷和灭多威的敏感性不同。在敏感品系中,取食不同食料的斜纹夜蛾后代对灭多威的敏感性顺序为:烟草<棉花<大豆<人工饲料;对丙溴磷而言,敏感性顺序则为:棉花<烟草<大豆<人工饲料。而在田间抗性品系中,对丙溴磷的敏感性顺序为:棉花<烟草<人工饲料<大豆;对灭多威的敏感性顺序为:棉花<烟草<大豆<人工饲料。此外,在田间抗性和敏感品系中,取食不同食料的斜纹夜蛾体内的酯酶活性之间均存在显著差异,对其乙酰胆碱酯酶的活性也有程度不同的诱导效应,但并不会引起乙酰胆碱酯酶的质变。  相似文献   

17.
Exposure of a spore-crystal suspension of Bacillus thuringiensis to UV irradiation for (200 lx) 8.5 min killed most of the spores (PP0 = 2.6 × 10?4), while the insecticidal activity of the suspension to larvae of Spodoptera littoralis was only slightly affected. Numbers of colony-forming units (CFU) of B. thuringiensis recovered from larvae after ingestion of spores decreased with time as long as the larvae lived and several hours after larval death. Only 3–6 hr after larval death, the spores germinated and multiplied, reaching up to 100-fold after 24 hr. When UV-irradiated suspensions were used, numbers of CFU per larva were too scarce to be recovered from living larvae. However, 1.5 × 106 CFU/larva were recovered 24 hr after death. It seems that the disruption of the gut epithelium by the endotoxin caused a change in the unfavorable conditions for endospore germination, thus providing the suitable ambient for germination and multiplication of B. thuringiensis. Numbers of other bacteria present per milligram of healthy larva increased with larval weight, predominantly Streptococcus sp. and Erwinia sp. In dead larvae, the increase of Erwinia sp. was higher than that of Streptococcus sp. Other bacterial species isolated were: Corynebacterium sp., Micrococcus sp., Serratia marcescens, and Bacillus sp.  相似文献   

18.
Toxicity assays indicated that when diflubenzuron (DFB) was topically applied on larvae of the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval), and the beet armyworm, Spodoptera exigua (Hübner), estimated LD50 values were similar, reaching 0.47 and 0.44 ng/larva, respectively. This study evaluated the importance of the rate of uptake and excretion, and of enzymatic metabolism in building up an insecticidal toxicity after topical application on the insect cuticle in both species. In general, penetration of DFB in S. littoralis was about 2-fold higher than in S. exigua, whereas metabolic breakdown was of minor importance in S. littoralis as compared with S. exigua. The current data indicate the importance of cuticle penetration and discuss that extrapolation from oral feeding toxicity results to topical toxicity data should be evaluated with caution, especially for insect growth regulators used in integrated pest management.  相似文献   

19.
The hypothesis that mature egg availability during a parasitoid's lifetime affects the functional response, as well as a parasitoid's handling time and searching efficiency, was tested. Campoletis grioti Blanchard (Hym: Ichneumonidae) female parasitoids and Spodoptera frugiperda Smith (Lep: Noctuidae) host larvae were used as an example. Each female of C. grioti was confined with different host densities (6, 10, 30, 50, 80, 120 and 160 larvae/day) of first and second S. frugiperda instars until the female died. We found that C. grioti showed a type II functional response, with a reduction in the number of parasitized hosts in relation to female ageing. Handling time and searching efficiency increased over time. When we standardized the data simulating unlimited egg supply, the functional response, handling time and searching efficiency became constant throughout the parasitoid's life. We discuss the relationship between egg availability and functional response and its associated parameters, and its consequence on biological control programs.  相似文献   

20.
钴对蔬菜毒害的临界值   总被引:4,自引:0,他引:4  
采用水培+土培的方法研究了钴对蔬菜的毒害临界值。首先从18种福建常见蔬菜中筛选出钴高敏感蔬菜,再通过土培试验研究土壤钴对高敏感蔬菜的毒害临界浓度。结果表明,在水培条件下钴对蔬菜生长存在较低浓度的刺激效应和高浓度的抑制效应。综合表观症状和蔬菜地上部生物量减少20%的效应浓度值(EC20)确定,早熟5号大白菜(Brassica pekinensis)和黄瓜(Cucumis sativus)为钴高敏感作物。以黄瓜为指示作物的土培试验表明,钴对黄瓜未表现出增产效应,随着钴添加量的增加,黄瓜地上部生物量显著下降。以黄瓜地上部减产10%和野外土壤有效钴和全钴的回归关系为依据,确定土壤钴毒害临界值为:土壤有效钴(DTPA)为2.7mg·kg-1,土壤全钴含量为98mg·kg-1。  相似文献   

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