共查询到20条相似文献,搜索用时 31 毫秒
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植物中DREBs类转录因子及其在非生物胁迫中的作用 总被引:3,自引:0,他引:3
低温、干旱、高盐等非生物胁迫能够严重影响植物的生长及作物的产量。最近发现了许多调控多种与逆境相关基因表达的转录因子, 其中DREBs类转录因子能够通过与含有DRE/CRT顺式作用元件的抗逆相关基因启动子区相互作用, 进而调控一系列抗逆基因的表达, 使植物品质得到综合改良从而提高植物对非生物胁迫耐受力。文章通过对DREBs的结构、表达调控、作用方式及机理进行总结, 并结合其在植物胁迫信号通路中的作用以及提高转基因植株胁迫耐受性的最新研究成果加以综述, 并对其在农业生产中的应用前景进行展望。 相似文献
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干旱严重影响植物的生长发育及农作物产量,因此研究植物的干旱反应机制显得至关重要.我们发现在拟南芥中一个推测的谷氨酸羧肽酶, AMP1, 其缺失突变体amp1的抗旱能力大大增强.基因芯片分析表明,amp1突变体抗旱能力的提高与许多干旱响应基因的高表达息息相关,例如,在amp1突变体中2个干旱诱导表达的转录因子基因,DREB2A和DREB1A的表达量升高;AT1G61340 (LEA 蛋白)的表达量也升高了很多,它在干旱条件下具有解毒和缓解细胞伤害的作用.而且,在amp1突变体中DREB2A 转录因子的2个下游基因RD29A 和COR47受干旱诱导的表达量和时间都比野生型中高和早. 在突变体中一些参与蛋白代谢、糖代谢和脂代谢的基因上调,一些保护和解毒相关基因表达量也升高,这些都可以给突变体在抗旱反应过程提供一定的保护作用.因此,我们认为AMP1基因在干旱胁迫反应中对干旱响应基因的表达起到一个负调控作用.实验中我们还发现, amp1突变体具有较低的水势与非常发达的根系,这也可能在抗旱反应中起到了一定作用. 相似文献
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Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
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Madoka Kudo Satoshi Kidokoro Takuya Yoshida Junya Mizoi Daisuke Todaka Alisdair R. Fernie Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《Plant biotechnology journal》2017,15(4):458-471
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HONG Bo TONG Zheng MA Nan LI Jianke KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko GAO Junping 《中国科学C辑(英文版)》2006,49(5):436-445
DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) ‘Fall Color’ genome. Compared with wild type plants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants. RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. The transgenic plants exhibited tolerance to drought and salt stress, and the tolerance was significantly stronger
in rd29A:DREB1A plants than in 35S:DREB1A plants. Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions. These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum, and improvement of the stress tolerance may be related
to enhancement of proline content and SOD activity. 相似文献
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Bo Hong Zheng Tong Nan Ma Jianke Li Mie Kasuga Kazuko Yamaguchi-Shinozaki Junping Gao 《中国科学:生命科学英文版》2006,49(5):436-445
DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) ‘Fall Color’ genome. Compared with wild type plants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants. RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. The transgenic plants exhibited tolerance to drought and salt stress, and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants. Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions. These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum, and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. 相似文献
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Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes 总被引:4,自引:0,他引:4
Satoko Matsukura Junya Mizoi Takumi Yoshida Daisuke Todaka Yusuke Ito Kyonoshin Maruyama Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki 《Molecular genetics and genomics : MGG》2010,283(2):185-196
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Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression 总被引:3,自引:0,他引:3
Qin F Sakuma Y Tran LS Maruyama K Kidokoro S Fujita Y Fujita M Umezawa T Sawano Y Miyazono K Tanokura M Shinozaki K Yamaguchi-Shinozaki K 《The Plant cell》2008,20(6):1693-1707
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Special trends in CBF and DREB2 groups in Eucalyptus gunnii vs Eucalyptus grandis suggest that CBF are master players in the trade‐off between growth and stress resistance
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Hong C. Nguyen Phi B. Cao Hélène San Clemente Raphaël Ployet Fabien Mounet Nathalie Ladouce Luc Harvengt Christiane Marque Chantal Teulieres 《Physiologia plantarum》2017,159(4):445-467
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