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1.
The presence of pathogenic microorganisms in municipal waste sludge may create a serious outbreak of water borne diseases if the sludge is used for agricultural purpose. An attempt to decrease the number of pathogenic microorganisms, Salmonella spp. using a simulated acid-phase anaerobic digester was tested in a laboratory-scale batch experiment. Reduction of Salmonella spp. was demonstrated in a mixture of sludge and organic acid, simulating an acid digester of a two-phase anaerobic digestion process. A high concentration of organic acid at a pH value of 5.5-6.0 prevents a decrease in Salmonella spp. concentration. Almost complete destruction of Salmonella spp. is observed within two days if the pH value is maintained below 5.5. 相似文献
2.
It is known that the presence of volatile fatty acids may play a role in the inactivation of pathogens for systems that employ an acid phase reactor. This study was conducted to investigate the influence of volatile fatty acids on the inactivation of Salmonella spp. over a range of digestion temperatures. In this study, digesters that were treating municipal wastewater treatment plant sludges were operated at temperatures that ranged from 35 to 49 degrees C and had a solids residence time of 15 days. Samples collected from the effluent of the digesters were dosed with solutions containing acetic, propionic, and butyric acids alone and in mixtures, and the dosed effluents were analyzed for Salmonella spp. over time. In the first round of testing, the digester effluents were dosed with individual organic acids and also a mixture containing all three volatile fatty acids over a range of concentrations from 750 to 6000 mg/L, and the pH of the samples was fixed at a value of 5.5. In the second round of testing, the sample sludges were spiked with a fixed amount of organic acid mixture, and the pH was varied from 4.5 to 7.5. The reduction of Salmonella spp. in digester effluents, when dosed with volatile organic acids, was found to depend on pH, temperature, the chain length of the acids, and the concentration and composition of the acids present. Increases in temperature appeared to increase the inhibitory effects of the volatile organic acids. At mesophilic temperatures, acidic pHs resulted in a greater inhibition of Salmonella spp.; whereas at higher temperatures neutral pHs were found to be more inhibitory. The results suggest that acid phase digesters that operate at elevated temperatures and low pH can achieve substantial reduction of Salmonella spp. 相似文献
3.
Hamidreza Salsali Wayne J. Parker Syed A. Sattar 《World journal of microbiology & biotechnology》2008,24(5):659-665
The application of sludge digestion systems to remove pathogens has been employed to generate biosolids suitable for reuse
in agriculture. Traditionally, temperature is considered the principal agent responsible for pathogen reduction in anaerobic
digestion. However, other substances such as volatile fatty acids may also have an antimicrobial effect. The objective of
this study was to assess the impact of fatty acid mixtures on the inactivation of C. perfringens over a range of digestion temperatures. An equimolar mixture of acetic acid, propionic acid and butyric acid was applied
to digester effluent for a period of 24 h at temperatures of 35 °C, 42 °C, 49 °C and 55 °C. C. perfringens inactivation in digester effluents, when dosed with volatile organic acids, was found to depend on pH, acid concentration
and temperature. Temperatures above 55 °C appeared to increase the inhibitory effects of the organic acids at higher concentrations.
An interaction between temperature and pH on survival of C. perfringens was observed. The results suggest that high concentrations of organic acids at a pH value of 4.5–5.5 during thermophilic
digestion substantially reduce concentrations of C. perfringens in municipal sludge. 相似文献
4.
Survival of bacterial pathogens during the thermophilic anaerobic digestion of biowaste: laboratory experiments and in situ validation 总被引:1,自引:0,他引:1
Anaerobic digestion is continually gaining importance for the processing of the organic fraction of municipal solid wastes. Although methods for studying the survival of pathogen exist, these methods often need adaptations, are expensive, time consuming or generally not well suited for the harsh conditions within an anaerobic digestion system. In the present study we investigated the applicability of commercially available, mechanically stable and inexpensive pathogen carriers to validate in situ pathogen inhibition within a 750,000l thermophilic, bio-waste treating anaerobic digester. None of the pathogens investigated (Listeria monocytogenes, Salmonella enterica, Escherichia coli, and Campylobacter jejuni) was capable of survival under the conditions of the biogas reactor for more than 24 h indicating that the temperature and physico-chemical properties of the sludge of the fermenter were effective in inhibiting the survival of these microorganisms. 相似文献
5.
Bacterial stress enrichment enhances anaerobic hydrogen production in cattle manure sludge 总被引:2,自引:0,他引:2
Methodology was evaluated to selectively enrich hydrogen-producing species present in biological sludge produced during organic wastewater treatment. The influence of bacterial stress enrichment on anaerobic hydrogen-producing microorganisms was investigated in batch tests using serum bottles. Enrichment conditions investigated included application of acute physical and chemical stresses: wet heat, dry heat and desiccation, use of a methanogen inhibitor, freezing and thawing, and chemical acidification with and without preacidification of the sludge at pH 3. For each enrichment sample, cultivation pH value was set at an initial value of 7. After application of selective enrichment (by bacterial stress), hydrogen production was significantly higher than that of untreated original sludge. Hydrogen production from the inocula with bacterial stress enrichment was 1.9–9.8 times greater when compared with control sludge. Chemical acidification using perchloric acid showed the best hydrogen production potential, irrespective of preacidification. Enhancement is due to the selective capture of hydrogen-producing sporeformers, which induces altered anaerobic fermentative metabolism. 相似文献
6.
Ethanol and lactic acid production from sugar and starch wastes by anaerobic acidification
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Anaerobic conversion of carbohydrates can generate various end‐products. Besides physical parameters such as pH and temperature, the types of carbohydrate being fermented influences the fermentation pattern. Under uncontrolled pH, microbial mixed cultures from activated sludge and anaerobic digester sludge anaerobically produced ethanol from glucose while producing lactic acid from starch conversion. This trend was not only observed in batch trials. Also, continuous chemostat operation of anaerobic digester sludge resulted in the reproducible predominance of ethanol fermentation from glucose solution and lactic acid production from starch. Different feeding regimes and substrate availability (shock load versus continuous feeding) in glucose fermentation under non‐controlled pH did not affect the ethanol production as the major end product. Shifts in feed composition from glucose to starch and vice versa result in an immediate change of fermentation end products formation. 相似文献
7.
The system investigated in this study is an anaerobic digester at a municipal wastewater treatment plant operating on sludge
from the wastewater treatment, co-digested with carbohydrate-rich food-processing waste. The digester is run below maximum
capacity to prevent overload. Process monitoring at present is not extensive, even for the measurement of on-line gas production
rate and off-line pH. Much could be gained if a better program for monitoring and control was developed, so that the full
capacity of the system could be utilised without the risk of overload. The only limit presently set for correct process operation
is that the pH should be above 6.8. In the present investigation, the pH was compared with alkalinity, gas production rate,
gas composition and the concentration of volatile fatty acids (VFA). Changes in organic load were monitored in the full-scale
anaerobic digester and in laboratory-scale models of the plant. Gas-phase parameters showed a slow response to changes in
load. The VFA concentrations were superior for indicating overload of the microbial system, but alkalinity and pH also proved
to be good monitoring parameters. The possibility of using pH as a process indicator is, however, strongly dependent on the
buffering capacity. In this study, a minor change in the amount of carbohydrates in the substrate had drastic effects on the
buffering effect of the system.
Received: 21 January 2000 / Received revision: 10 July 2000 / Accepted: 16 July 2000 相似文献
8.
Survival of pathogenic bacteria during mesophilic anaerobic digestion of animal waste 总被引:3,自引:1,他引:2
Theresa E. Kearney M.J. Larkin J.P. Frost P.N. Levett 《Journal of applied microbiology》1993,75(3):215-219
The survival of pathogenic bacteria was investigated during the operation of a full-scale anaerobic digester which was fed daily and operated at 28°C. The digester had a mean hydraulic retention time of 24 d. The viable numbers of Escherichia coli, Salmonella typhimurium, Yersinia enterocolitica, Listeria monocytogenes and Campylobacter jejuni were reduced during mesophilic anaerobic digestion. Echerichia coli had the smallest mean viable numbers at each stage of the digestion process. Its mean T90 value was 76.9 d. Yersinia enterocolitica was the least resistant to the anaerobic digester environment; its mean T90 value was 18.2 d. Campylobacter jejuni was the most resistant bacterium; its mean T90 value was 438.6 d. Regression analysis showed that there were no direct relationships between the slurry input and performance of the digester and the decline of pathogen numbers during the 140 d experimental period. 相似文献
9.
The effect of iron on anaerobic digestion 总被引:1,自引:0,他引:1
Summary The ability of methanogenic bacteria to adapt to high concentrations of iron was investigated using a 9l Upflow Anaerobic Sludge Blanket (UASB) reactor fed semi-continuously with a synthetic waste containing glucose as the organic carbon source. It was found that an iron concentration of up to 5 650 mg/l, which is well in excess of previously reported toxicity levels, had no inhibitory effects on anaerobic digestion, with the exception of a decrease in biogas production. The iron precipitated out and accumulated in the sludge bed of the digester, resulting in very low concentrations of iron in the digester effluent. 相似文献
10.
《Critical reviews in biotechnology》2013,33(4):448-460
The thermophilic anaerobic digestion (TAD) of sewage sludge has often been found to be less stable than mesophilic treatment. In comparison to mesophilic digesters, thermophilic reactors treating sludge are generally characterized by relatively high concentrations of volatile fatty acids (VFA) in the effluent along with poor effluent quality, indicating a lower level of process stability. However, reviewing the literature related to the procedure for obtaining a thermophilic inoculum, it seems that most of the problems associated with the instability and the accumulation of organic intermediates are the result of the manner in which the thermophilic sludge has been obtained. In this paper, the different options available for obtaining an anaerobic digester operating at thermophilic temperature (55°C) have been reviewed. In this light, rapid heating to the target temperature followed by the development of thermophilic microorganisms, which can be determined by VFA dropping to ≤500?mg acetic acid L?1 before increasing the organic loading rate (OLR), has been determined the most suitable means of establishing TAD. 相似文献
11.
The objective of this study was to characterize the toxic effects of three well known thiol-reactive electrophilic compounds, N-ethylmaleimide (NEM), pentachlorophenol (PCP) and 1-chloro-2,4-dinitrobenzene (CDNB) on anaerobic biotransformation process. The work was part of a larger investigation on potassium efflux as a possible response mechanism of anaerobic microorganisms to the presence of thiol-reactive organic compounds and the interference of such compounds on the reductive dehalogenation process. Using anaerobic toxicity assay (ATA) and granular anaerobic biomass from a full-scale upflow anaerobic sludge blanket (UASB) reactor, inhibitory concentrations of these compounds that reduced the microbial activity of granular biomass to 50% of a control (IC50) were determined to be 592, 0.97, and 450 mg/l for NEM, PCP, and CDNB, respectively. Toxicity of NEM was also tested on anaerobic biomass from a municipal wastewater treatment plant digester and slightly lower IC50 of 532 mg/l was obtained. The results presented here indicate that anaerobic biomass can acclimate to the three thiol-reactive compounds studied and recover from inhibition as long as the toxicant concentration is below a threshold level. That threshold concentration was found to be 500 mg/l for NEM on biomass from the municipal digester, 1 mg/l for PCP, and 500 mg/l for CDNB, both on granular biomass. Granular anaerobic biomass showed recovery even at NEM concentrations of 1000 mg/l. 相似文献
12.
An integrated UASB-sludge digester system for raw domestic wastewater treatment in temperate climates 总被引:1,自引:0,他引:1
To improve the performance of an upflow anaerobic sludge blanket (UASB) reactor treating raw domestic wastewater under temperate climates conditions, the addition of a sludge digester to the process was investigated. With the decrease in temperature, the COD removal decreased from 78% at 28 °C to 42% at 10 °C for the UASB reactor operating alone at a hydraulic retention time of 6 h. The decrease was attributed to low hydrolytic activity at lower temperatures that reduced suspended matter degradation and resulted in solids accumulation in the top of the sludge blanket. Solids removed from the upper part of the UASB sludge were treated in an anaerobic digester. Based on sludge degradation kinetics at 30 °C, a digester of 0.66 l per liter of UASB reactor was design operating at a 3.20 days retention time. Methane produced by the sludge digester is sufficient to maintain the temperature at 30 °C. 相似文献
13.
Summary Microcalorimetrically determined heat production was used as parameter for microbial metabolism to study some aspects of anaerobic digestion.The heat production of sludge samples from a sufficiently active anaerobic digester is dependent upon the concentration of organic substances and is correlated with dehydrogenase activity.Microcalorimetric measurements were carried out to investigate the metabolisation of added substrates; H-values of 150–270 kJ/M of cellobiose were determined for the digestion of cellobiose. Heat flow during the adaptation of digester sludge to cellobiose showed a dramatic change.The action of heavy metals on the microcalorimetrically determined metabolic activity of sludge samples was tested and we show that the addition of heavy metals, in concentrations often detected in waste water treatment, reduced the heat flow by up to 75%. 相似文献
14.
Composting is increasingly used to transform biosolids, obtained following wastewater treatment, into a more stable organic product that can be released in the environment. The process must however be closely monitored to assure that the end product meets the regulations set by environmental agencies with regards to the amount of pathogenic microorganisms present. In this study, a TaqMan qPCR approach targeting the invA gene was developed to monitor the presence of Salmonella spp. in composted biosolids. A validation step was first performed to evaluate the effect of compost age on the quantification of various concentrations of seeded Salmonella typhimurium. Secondly, qPCR was used to investigate the effect of composting time, varying from 1 month to 24 months, on the presence of Salmonella spp. naturally present in biosolids samples. Culture media were used in parallel to corroborate the results obtained by qPCR. The detection limit of the invA gene obtained experimentally from composts seeded with S. typhimurium was 5.8 copies or the equivalent of 5.8 CFU per qPCR reaction. Although the results indicated that compost age had a marginal effect on the detection of seeded S. typhimurium, the TaqMan qPCR approach was efficient at detecting and quantifying the amount of Salmonella spp. present in naturally contaminated composted biosolids of different ages. Results showed that there was a significant decrease in the amount of Salmonella DNA present in composted biosolids over time, which was also corroborated by the CFU counts obtained on the BSA culture medium. However, qPCR was more specific, robust and rapid to execute than performing counts on culture media. qPCR shows promise for routine examination of composted biosolids to ascertain that pathogenic microorganisms, including Salmonella spp., are decreased below acceptable limits before their application in the environment. 相似文献
15.
Reductive dechlorination of carbon tetra-chloride (CCl4) by anaerobic bacterial communities from anaerobic digester sludge with the amendment of low concentrations of electron donors
and microorganisms was undertaken to evaluate the influence of electron donors and microbial concentration on the rate of
dechlorination of CCl4. Humic acid, acetate, and glucose were selected to examine the feasibility of the electron donor with respect to the remediation
of a contaminated subsurface. The addition of an electron donor and microorganisms significantly enhanced the dechlorination
rate of carbon tetrachloride. The addition of an electron donor increased the cell numbers of anaerobic consortia, thereby
increasing the rate of dechlorination. Glucose was a better electron donor than acetate and humic acid under reducing environments.
The pseudo-first-order degradation rate constants of CCl4 ranged from 0.0057 day-1 to 0.135 day-1, depending on the conditions of the electron donor and biomass supplemented. Furthermore, the addition of the electron donor
in the batches amended with 0.56 mg volatile suspended solids (VSS)/l biomass had a higher enhanced efficiency than those
with 1.7 mg VSS/l biomass. These results suggest that there is a potential for stimulating the dechlorinating capability of
anaerobic consortia to remedy the chlorinated hydrocarbons in the oligotrophic environment if the conditions of the supplementing
electron donor are properly selected.
Received: 14 August 1995/Received last revision: 15 March 1996/Accepted: 15 April 1996 相似文献
16.
In order to reduce the discharge of residual sludge from an anaerobic digester, pre-treatment methods including low-pressure wet-oxidation, Fenton oxidation, alkali treatment, ozone oxidation, mechanical destruction and enzymatic treatment were evaluated and compared. VSS removal efficiencies of greater than 50% were achieved in cases of low-pressure wet-oxidation, Fenton oxidation and alkali treatment. Residual sludge from an anaerobic digester was pre-treated and subjected to thermophilic anaerobic digestion. As a result, the process of low-pressure wet-oxidation followed by anaerobic digestion achieved the highest VSS removal efficiency of 83%. The total efficiency of VSS removal of sewage sludge consisting of primary and surplus sludge would be approximately 92%, assuming that the VSS removal efficiency of sewage sludge is 50% in the anaerobic digester of the sewage treatment plant. 相似文献
17.
Inactivation of Salmonella typhi by high levels of volatile fatty acids during anaerobic digestion 总被引:1,自引:0,他引:1
Survival of Salmonella typhi was investigated in an anaerobic digester for cattle dung with volatile fatty acid (VFA) levels of 5000 mg l(-1) and pH 6.0. The organism was added to the digester only once in the first experiment and daily in the other. Survival was monitored on alternate days. In the single dose experiment, the counts of Salm. typhi declined rapidly and the pathogen was completely eliminated within 12 d in the experimental digester (VFA ca 5000 mg l(-1) and pH 6.0), whereas 26 d were required in the control digester (VFA ca 100 mg l(-1) and pH 6.8). T90 values for the experimental and control digesters were 2.44 d and 4.80 d, respectively. In the daily dose experiment, a four log reduction in the pathogen count was observed in the experimental digester, but only a two log reduction in the control digester at the end of the experimental period. The mean T90 values for the experimental and the control digester were 4.22 d and 18.63 d, respectively. In both the experiments, statistical analysis of the data showed significant differences in the survival pattern of Salm. typhi in the two digesters. 相似文献
18.
19.
Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge. 总被引:4,自引:28,他引:4
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The kinetics of propionate degradation, acetate splitting, and hydrogen consumption in digesting sludge were investigated in a lab-scale digester. At natural steady-state conditions, the acetate-splitting systems in well-digested sludge were about half saturated. Propionate-degrading systems were saturated to only 10 to 15%, and hydrogen removal was less than 1% of the maximum possible rate. It was concluded that acetate splitting rather than "methanogenesis from fatty acids" is the rate-limiting reaction in the anaerobic degradation of dissolved organic matter and that a methoanogenic anaerobic ecosystem is stabilized by its large unused capacity of hydrogen consumption which is "buffering" the partial pressure of dissolved hydrogen in the system at sufficiently low values to permit rapid fatty acid oxidation. A tentative scheme of the substrate flow in sludge digestion is presented. It suggests that acid formation coupled with hydrogen formation via pyridine dinucleotide oxidation yields the immediate substrates, namely acetate and hydrogen, for about 54% of the total methanogenesis. 相似文献
20.
Yu. V. Litti V. K. Nekrasova N. I. Kulikov M. V. Siman’kova A. N. Nozhevnikova 《Microbiology》2013,82(6):690-697
Attached activated sludge from the Krasnaya Polyana (Sochi) wastewater treatment plant was studied after the reconstruction by increased aeration and water recycle, as well as by the installation of a bristle carrier for activated sludge immobilization. The activated sludge biofilms developing under conditions of intense aeration were shown to contain both aerobic and anaerobic microorganisms. Activity of a strictly anaerobic methanogenic community was revealed, which degraded organic compounds to methane, further oxidized by aerobic methanotrophs. Volatile fatty acids, the intermediates of anaerobic degradation of complex organic compounds, were used by both aerobic and anaerobic microorganisms. Anaerobic oxidation of ammonium with nitrite (anammox) and the presence of obligate anammox bacteria were revealed in attached activated sludge biofilms. Simultaneous aerobic and anaerobic degradation of organic contaminants by attached activated sludge provides for high rates of water treatment, stability of the activated sludge under variable environmental conditions, and decreased excess sludge formation. 相似文献