共查询到20条相似文献,搜索用时 15 毫秒
2.
The importance of soybean as a source of oil and protein, and its ability to grow symbiotically on low-N soils, point to its continued status as the most valuable grain legume in the world. With limited new land on which to expand, and emphasis on sustainable systems, increases in soybean production will come mostly from increased yield per unit area. Improvements in biological nitrogen fixation can help achieve increased soybean production, and this chapter discusses research and production strategies for such improvement.The soybean-Bradyrhizobium symbiosis can fix about 300 kg N ha -1 under good conditions. The factors which control the amount of N fixed include available soil N, genetic determinants of compatibility in both symbiotic partners and lack of other yield-limiting factors. Response to inoculation is controlled by the level of indigenous, competing bradyrhizobia, the N demand and yield potential of the host, and N availability in the soil.Research efforts to improve BNF are being applied to both microbe and soybean. While selection continues for effective, naturally occurring bradyrhizobia for inoculants and the use of improved inoculation techniques, genetic research on bradyrhizobia to improve effectiveness and competitiveness is advancing. Selection, mutagenesis and breeding of the host have focused on supernodulation, restricted nodulation of indigenous B. japonicum, and promiscuous nodulation with strains of bradyrhizobia from the cowpea cross-inoculation group. The research from the host side appears closer to being ready for practical use in the field.Existing knowledge and technology still has much to offer in improving biological nitrogen fixation in soybean. The use of high-quality inoculants, and education about their benefits and use can still make a significant contribution in many countries. The importance of using the best adapted soybean genotype with a fully compatible inoculant cannot be overlooked, and we need to address other crop management factors which influence yield potential and N demand, indirectly influencing nitrogen fixation. The implementation of proven approaches for improving nitrogen fixation in existing soybean production demands equal attention as received by research endeavours to make future improvements. 相似文献
3.
Soybean plants [ Glycine max (L.) Merr.] were grown in silica sand and were drought stressed for a 4 week period during reproductive development and without any mineral N supply in order to maximize demand for fixed nitrogen. A strain of Bradyrhizobium japonicum that forms large quantities of polysaccharide in nodules was used to determine whether or not the supply of reduced carbon to bacteroids limits nitrogenase activity. A depression of 30–40% in nitrogen content in leaves and pods of stressed plants indicated a marked decline in nitrogen fixation activity during the drought period. A 50% increase in the accumulation of bacterial polysaccharide in nodules accompanied this major decrease in nitrogen fixation activity and this result indicates that the negative impact of drought on nodules was not due to a depression of carbon supply to bacteroids. The drought treatment resulted in a statistically significant increase in N concentration in leaves and pods. Because N concentration and chlorophyll concentration in leaves were not depressed, there was no evidence of nitrogen deficiency in drought‐stressed plants, and this result indicates that the negative impact of drought on nodule function was not the cause of the depression of shoot growth. At the end of the drought period, the concentration of carbohydrates, amino nitrogen, and ureides was significantly increased in nodules on drought‐stressed plants. The overall results support the view that, under drought conditions, nitrogen fixation activity in nodules was depressed because demand for fixed N to support growth was lower. 相似文献
4.
Summary Isotopic as well as non-isotopic methods were used to assess symbiotic nitrogen fixation within eight soybean [ Glycine max (L.) Merr.] cultivars grown at 20 and 100 kg N/ha levels of nitrogen fertilizer under field conditions.The 15N methodology revealed large differences between soybean cultivars in their abilities to support nitrogen fixation. In almost all cases, the application of 100 kg N/ha resulted in lower N 2 fixed in soybean than at 20 kg N/ha in the first year of the study. However, N 2 fixed in one cultivar, Dunadja, was not significantly affected by the higher rate of N fertilizer application. These results were confirmed by measurements of acetylene reduction activity, nodule dry weight and N 2 fixed as measured by the difference method. Further proof of differences in N 2 fixed within soybean cultivars and the ability of Dunadja to fix similar amounts of N 2 at 20 and 100 kg N/ha was obtained during a second year experiment. Dunadja yield was affected by N fertilizer and produced larger yield at 100 kg N/ha than at 20 kg N/ha. This type of cultivar could be particularly useful in situations where soil N levels are high or where there is need to apply high amounts of N fertilizer.The present study reveals the great variability between legume germplasms in the ability to fix N 2 at different inorganic N levels, and also the potential that exists in breeding for nitrogen fixation associative traits. The 15N methodology offers a unique tool to evaluate germplasms directly in the field for their N 2 fixation abilities at different N fertilizer levels. 相似文献
5.
Nodulated soybean plants ( Glycine max (L.) Merr. cv. Clarke)were supplied with 10 mol m -3 nitrate at the vegetative stage.This treatment caused a rapid decline in nitrogen fixation (acetylenereduction) activity and a consequent decline in ureides in thexylem sap. However, there was virtually no effect on the nitrogenasecomplex, according to Western blots against components 1 and2. The effect on nitrogen fixation was matched by a decreasein nitrogenase-linked respiration and increases in nodule oxygendiffusion resistance and the carbon cost of nitrogen fixation.The addition of nitrate had little effect on protein contentfrom either nodule plant or bacteroid fractions. Activitiesof nitrate reductase (NR) and nitrite reductase (NiR) from eitherthe plant fraction or the bacteroids were affected in differentways during 8 d of supply. Nodule plant NR and bacteroid NiR were not affected. However,nodule plant NiR increased 5-fold within 2 d of supplying Bacteroid NR only increased after6 d. These results could be interpreted in terms of a restrictednitrate access into the infected region of nodules. However,denitrification was detected within 2 d of nitrate supply insoybean nodules. The results are discussed in relation to possiblecauses of the nitrate-induced decline in nitrogenase activity. Key words: Glycine max, nitrate, nitrogen fixation, nodules 相似文献
6.
Fertilizer N use in Japan has decreased by about 30% from 1960 to 2000, while keeping a little increase in cereal yields. This has resulted in a significant increase in apparent nitrogen use efficiency, in particular for rice. On the other hand, national N load on the environment associated with the production and consumption of domestic and imported agricultural products has almost tripled during this period, mainly due to the dramatic increase of imports of food and feedstuffs. The environmental problems, including water and air pollution, caused by the excessive loads of N are serious public concerns and there is an urgent need to minimize N losses from agricultural production. In order to meet the necessity for reducing the environmental impacts by excess N, political and technological measures have been taken at regional and country levels. In recent years, the Japanese government has embarked on a series of policies to encourage transition to an environmentally conscious agriculture. Promoting proper material circulation with reducing fertilizer impact and utilizing biomass and livestock wastes is emphasized in these policies. The effectiveness of environmental assessment and planning for reducing regional and national N load has been discussed. Implementation of environmentally friendly technologies and management, both conventional and innovational, have been developed and adopted in Japanese agriculture. The effectiveness of conventional technologies in reducing environmental reactive N has been re-evaluated. Innovative technologies, such as use of controlled availability fertilizers and livestock wastes compost pellets, are being investigated and extended. A comprehensive approach that applies political and technological measures with closer cooperation is necessary to control reactive N in the environment. 相似文献
8.
Fertilizer N use in Japan has decreased by about 30% from 1960 to 2000, while keeping a little increase in cereal yields. This has resulted in a significant increase in apparent nitrogen use efficiency, in particular for rice. On the other hand, national N Icad on the environment associated with the production and consumption of domestic and imported agricultural products has almost tripled during this period, mainly due to the dramatic increase of imports of food and feedstuffs. The environmental problems, including water and air pollution, caused by the excessive loads of N are serious public concerns and there is an urgent need to minimize N losses from agricultural production. In order to meet the necessity for reducing the environmental impacts by excess N, political and technological measures have been taken at regional and country levels. In recent years, the Japanese government has embarked on a series of policies to encourage transition to an environmentally conscious agriculture. Promoting proper material circulation with reducing fertilizer impact and utilizing biomass and livestock wastes is emphasized in these policies. The effectiveness of environmental assessment and planning for reducing regional and national N Icad has been discussed. Implementation of environmentally friendly technologies and management, both conventional and innovational, have been developed and adopted in Japanese agriculture. The effectiveness of conventional technologies in reducing environmental reactive N has been re-evaluated. Innovative technologies, such as use of controlled availability fertilizers and livestock wastes compost pellets, are being investigated and extended.A comprehensive approach that applies political and technological measures with closer cooperation is necessary to control reactive N in the environment. 相似文献
9.
Fertilizer N use in Japan has decreased by about 30% from 1960 to 2000, while keeping a little increase in cereal yields. This has resulted in a significant increase in apparent nitrogen use efficiency, in particular for rice. On the other hand, national N load on the environment associated with the production and consumption of domestic and imported agricultural products has almost tripled during this period, mainly due to the dramatic increase of imports of food and feedstuffs. The environmental problems, including water and air pollution, caused by the excessive loads of N are serious public concerns and there is an urgent need to minimize N losses from agricultural production. In order to meet the necessity for reducing the environmental impacts by excess N, political and technological measures have been taken at regional and country levels. In recent years, the Japanese government has embarked on a series of policies to encourage transition to an environmentally conscious agriculture. Promoting proper material circulation with reducing fertilizer impact and utilizing biomass and livestock wastes is emphasized in these policies. The effectiveness of environmental assessment and planning for reducing regional and national N load has been discussed. Implementation of environmentally friendly technologies and management, both conventional and innovational, have been developed and adopted in Japanese agriculture. The effectiveness of conventional technologies in reducing environmental reactive N has been re-evaluated. Innovative technologies, such as use of controlled availability fertilizers and livestock wastes compost pellets, are being investigated and extended. A comprehensive approach that applies political and technological measures with closer co-operation is necessary to control reactive N in the environment. 相似文献
10.
Adequate supplies of phosphorus (P) and iron (Fe) to legumes have been shown to be crucial in obtaining high nitrogen fixation rates and growth. These responses are anticipated as a result of the high requirement for P in energy transfer processes in the nodule and for Fe as a constituent of nitrogenase and leghemoglobin. However, little attention has been given to documenting the response of nitrogen fixation rates resulting from concentrations of P and Fe that actually exist in nodules. In particular, an open question is whether there is an interaction between nodule P and Fe concentrations that maximize nitrogen fixation activity. This study was designed to induce various concentrations of P and Fe in the nodule and to measure the resultant nitrogen accumulation and nitrogen fixation rates. Plant nitrogen accumulation was linearly correlated with both nodule P and Fe concentration, and with total plant nitrogen fixation rate as measured by acetylene reduction rate. Therefore, total nitrogen fixation rate was also correlated with nodule P and Fe concentrations, but a higher linear correlation was obtained for Fe as compared to P concentration. Surprisingly, nodule ureide concentration, which is generally assumed to be a positive index of nitrogen fixation rate, was negatively correlated with nodule P and Fe concentrations. These results indicated that higher concentrations of P and Fe in the nodules not only stimulated higher nitrogen fixation rates, but were associated with an enhanced ability to export ureides from the nodules. Since there was a linear response to both P and Fe over the range of nodule concentrations induced in these experiments, no evidence for optimum interactive concentrations of these two elements in the nodules was obtained. 相似文献
11.
Summary A system for employing open-ended root chambers to measure in situ acetylene reduction rates under field conditions is described. Gas mixtures containing about 2 mbar acetylene were continuously
flowed through the chambers providing a continuous record of acetylene reduction. These chambers have been used to measure
acetylene reduction rates of soybeans during three growing seasons. The system has proved to be reliable with a high degree
of precision. The large amount of plant-to-plant variability observed in N 2 fixation research has been confirmed by the data collected with this system. However, such variability in physiological studies
can be reduced by using a non-destructive system to compare the response of an individual plant with its rates before treatment. 相似文献
12.
Soybeans grown with 2 millimolar NO 3−, which optimized apparent N 2 fixation by Rhizobium symbionts, showed significantly different rates of apparent photosynthesis and C 2H 2 reduction during seedling development at two irradiances. Those physiological processes were lower for several weeks in plants grown at 1,500 microeinsteins per meter 2 per second than in those exposed to 700 microeinsteins per meter 2 per second. The irradiance-induced retardation was evident in short-term rates of apparent photosynthesis and N 2 fixation, as well as in measures of dry matter and total N accumulation. In spite of their previously inhibited development, plants grown at 1,500 microeinsteins per meter 2 per second were indistinguishable by day 28 from those exposed to 700 microeinsteins per meter 2 per second in terms of whole-shoot CO 2-exchange rate; by day 35 they were identical in terms of whole-plant C 2H 2-reduction rate. On day 38 there was no significant difference in dry weight or N content between treatments. Shifting plants between irradiance treatments on day 21 showed that the higher irradiance also had a short-term inhibitory effect on C 2H 2 reduction. The fact that 16 millimolar NO 3− prevented the continuous exposure to 1,500 microeinsteins per meter 2 per second from inhibiting apparent photosynthesis suggested that seedlings grown on 2 millimolar NO 3− with Rhizobium were N-limited. Although rates of apparent photosynthesis were similar by day 28, the additional week required to produce equal rates of apparent N 2 fixation between irradiance treatments showed that physiological adaptations of shoots, as well as photosynthesis per se, can affect root nodule activity. 相似文献
13.
Summary
Rhizobium japonicum strain 8-0 Str R applied as inoculum to Clark 63 soybeans formed small ineffective nodules which had very low nitrogenase activity compared to nodules formed by two effective strains, 110 Tet R and 138 Kan R. Mean numbers of cells per milligram of nodule tissue for plants up to 34 days old were 7.7×10 6 for 8-0 Str R, 4.1×10 8 for 110 Tet R and 7.6×10 8 for 138 Kan R. Cell counts per unit mass of nodule were independent of plant age for strains 110 Tet R and 138 Kan R, however, for strain 8-0 Str R, 25 and 34 days old plants had fewer viable cells per nodule mass than 18 day old plants. When a mixture of two effective strains was used, the nodules of individual plants were predominantly caused by either 110 Tet R or 138 Kan R. In one experiment the predominance was random, but in another, strain 110 Tet R clearly dominated. Strain 138 Kan R was absent in some nodules on 18 day old plants, and in others, less than 10 2 cells per nodule were found. When strains 8-0 Str R and 138 Kan R were used as mixed inoculum, most of the nodules had strain 8-0 Str R but strain 138 Kan R was detected in many nodules and was generally evident in the largest nodules. Nitrogenase activity by many individual nodules was low except for nodules which had cells of 138 Kan R. Nitrogenase activity by whole root systems of these plants was relatively high and similar to plants that had only nodules of strain 138 Kan R. Similar relationships were observed for a mixed inoculum of 8-0 Str R and 110 Tet R. In general, mixed inoculations resulted in nodules with a particular strain being dominant for each individual plant. Double infections within individual nodules were not uncommon and such nodules often had disproportionate numbers of cells of two competing R. japonicum strains.Contribution from the Laboratory of Soil Microbiology, Department of Agronomy, Missouri Agricultural Experiment Station. Missouri Journal Series Number 7967. 相似文献
14.
Membrane lipids in soybean nodules may undergo oxidative degradation resulting in the loss of membrane structural integrity and physiological activities. One of the final products of lipid peroxidation is malondialdehyde (MDA), which can react with thiobarbituric acid (TBA) in vitro to form a chromogenic adduct, a Schiff base product that can be measured spectrophotometrically. MDA formation was quantified in the nodules as well as in the adjacent root tissue. Lipid peroxidation was initially high in soybean nodules induced by Bradyrhizobium japonicum, but sharply declined following an increase in both leghemoglobin content and nitrogen fixation rate. Lipid peroxidation was 2 to 4 times higher in the nodules than in their corresponding adjoining root tissue. Malondialdehyde levels in ineffective nodules were 1.5 times higher than those in effective nodules. MDA formation was also shown to occur in the ‘leghemoglobin-free’ cytosolic fraction, the ‘leghemoglobin’ fraction, and the nodule tissue pellet. Antioxidants, such as reduced ascorbic acid, glutathione, and 8-hydroxyquinoline, caused a partial suppression of lipid peroxidation, whereas ferrous sulfate, hydrogen peroxide, iron EDTA, disodium-EDTA, and β-carotene induced MDA formation. In contrast, quenchers of oxygen free radicals such as HEPES, MES, MOPS, PIPES, phenylalanine, Tiron, thiourea, sodium azide, and sodium cyanide (uncouplers of oxidative phosphorylation) caused somewhere between a 12 to 70 percnt; reduction in MDA production. TBA-reactive products were formed despite the incorporation of superoxide dismutase, proxidase, and catalase into the reaction mixture. 相似文献
16.
Pigment biosynthesis in the cyanobacterium, Anacystis nidulans, was examined in the presence of gabaculine (5-amino-1,3-cyclohexadienyl-carboxylic acid). At 20 micromolar, this inhibitor blocked the biosynthesis of both chlorophyll and phycocyanin. Analogs of gabaculine were not effective as inhibitors of chlorophyll or phycocyanin biosynthesis. Iron- and phosphate-deficient cultures were 2- to 4-fold more sensitive to the inhibitor than were normal or nitrate-deficient cultures. Inhibition resulted in the excretion of a mixture of organic acids by the cells. δ-Aminolevulinic acid was a principle component of the mixture, identified by thin layer chromatography. Excretion of δ-aminolevulinic acid occurred following a brief lag after gabaculine addition. It remained linear for nearly 24 hours and was dependent upon illumination. However, high light inhibited excretion. Apparently, gabaculine blocks chlorophyll biosynthesis after the formation of δ-aminolevulinic acid in cyanobacteria. 相似文献
17.
Nitrogen fixation of terrestrial legumes is strongly and rapidly diminished under flooding. Although recovery is possible with the formation of aerenchyma, information is scarce regarding recovery after draining following short-term flooding, before the appearance of morphological adaptations. This study used soybean ( Glycine max) plants nodulated with Bradyrhizobium elkanii to determine xylem sap glutamine as an indication of nitrogen fixation activity during recovery from different periods of flooding. Xylem glutamine levels showed rapid recovery (within 90 min) following periods of flooding up to 4 h. Recovery was progressively slower after longer periods of flooding. After 48 h flooding very little recovery could be observed within the first 120 min after draining but recovery was possible within 48 h. Consistent with the changes in xylem glutamine, direct measurements of apparent nitrogenase activity carried out immediately on draining revealed rapid recovery after flooding for 1 h and slow recovery following 48 h of flooding. In the latter case, nitrogenase activity largely recovered 24 h after draining. Experiments with 15N 2 incorporation into amino acids exported in the xylem sap revealed that glutamine was by far the most highly labelled amino acid in sap collected over the first 30 min of exposure to the isotope. This is conclusive evidence that xylem sap glutamine is an immediate product of N 2 fixation and export. The changes in xylem sap glutamine seen on flooding (decline) and after draining (recovery) can therefore be attributed to changes in nitrogenase activity. The data show that xylem sap glutamine is a useful means for assessing changes in nitrogenase activity, especially when the root system is submersed in water and activity cannot be measured directly. 相似文献
18.
The growth stage of a soybean [Glycine max (L.) Merrill] plant may influence its physiological response to dark chilling. Opposed to vegetative development, the intense nutrient and energy requirements of the developing seeds during pod filling could cause additional chilling damage and decreased recovery capacity. Previously, we investigated dark chilling tolerance during vegetative development in two soybean genotypes, 'Maple Arrow' and 'Fiskeby V' and consistently found that photosynthesis and symbiotic nitrogen fixation (SNF) was less affected by dark chilling in 'Maple Arrow'. In this study we describe the dark chilling response of the same genotypes during pod filling. Our aim was to establish whether the potential selection criteria for dark chilling tolerance, identified during vegetative development, was equally sensitive during pod filling. The results indicate that photosynthesis is less affected by dark chilling in 'Maple Arrow' than in 'Fiskeby V', not only during vegetative development, but also during the critical reproductive stage of pod filling. 'Fiskeby V' also lacks the ability to restore normal photosynthetic capacity during an extended recovery treatment. The decrease of nodule ureide content indicates that SNF was inhibited to a similar extent in both genotypes. Nodule ureide content was reduced more than stem ureide content, suggesting that the former is a more sensitive indicator of chilling stress effects on SNF. The results indicate that certain photosynthetic and fluorescence parameters are sensitive indicators of dark chilling tolerance throughout plant development and should prove valuable in future breeding programmes aimed at increasing the chilling tolerance of soybean. 相似文献
19.
Eighty soybean cultivars were assessed for their potential for nodulation and nitrogen fixation with indigenous rhizobia in a Nigerian soil. Seventy-six days after planting (DAP) 87%, 3% and 10% of the soybean cultivars had from 0 to 30, 31 to 60 and over 61 nodules/plant, respectively. Only 8% had a nodule dry weight of 600 to 1100 mg/plant. At 84 DAP the proportion of nitrogen derived from the atmosphere (Ndfa) ranged from 0 to 65% 16% of the cultivars derived 51 to 65% of their N 2 from the atmosphere. The diversity of soybean germplasm and the variation in nodulation and N 2 fixation permitted the selection of the five best cultivars in terms of their compatibility with indigenous rhizobia, % Ndfa and the amount of N 2 which they fixed. 相似文献
20.
Vegetable soybeam germplasm was screened for its tolerance to 0, 50 and 100 μ M Al in solution culture. Plants were inoculated with prescreened acid-Al tolerant Bradyrhizobium japonicum strain USDA 110 and a local Rhizobium isolate SM867. Aluminum concentrations of 0, 50, and 100 μ M affected the root lengths of all germplasm lines in the first few weeks of their growth. At 100 μ M, the plants had severely stunted roots throughout the growing period of 35 days, but at 50 μ M the initial stunting of the roots was overcome after the third week of growth, and there were no significant differences
between the root lengths of these plants and of the controls. The appearance of the first nodule was delayed for 2–3 and 4–5
days at 50 μ M and 100 μ M Al, respectively. There was a significant reduction in nodule numbers and acetylene reduction activity (ARA) at 100 μ M Al. At 50 μ M Al, even though the number of nodules was decreased significantly, nodules were larger in size, so there was no significant
reduction in nodule fresh weight and ARA. No significant differences in nitrogen fixing abilities of the soybean lines were
observed between the two Rhizobium strains. Germplasm line Kahala showed the greatest tolerance to 50 μ M Al, and Kahala, Kim and Wolverine tolerated 100 μ M Al better than other germplasm lines. 相似文献
|