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1.
《Biometric Technology Today》2003,11(10):5
Including information on:
- ScanSoft
- SpeechWorks International
- Viisage Technology
- Firstec
- BIO-key International
- HP
- ZN Vision Technologies
- Unisys
- US Government’s
- Communication Intelligence Corporation
- Infinity Technologies
2.
《Biometric Technology Today》2003,11(11):6
- Daon
- Musicrypt
- EMI Music Canada
- Digital Broadband Networks
- FaceKey Corporation
- Eystar Media Inc (EMI)
- Temasya Wira
- Animated Electronic Industries
- BIO-key International
- Entryport Corporation
3.
《Biometric Technology Today》2003,11(10):3
Including information on:
- Martin State Airport
- Bioscrypt
- Saflink
- Office of the Secretary of Defense
- Department of Defense
- Boeing Corporation
- Bell ID, Gemplus
- Siemens
- Foreign Ministry
4.
《Biometric Technology Today》2003,11(9):5
- Bioscrypt
- Saflink
- Dell
- Fujitsu Microelectronics America
- Identix
- Viisage
- Acsys Biometrics
- US Government
5.
《The International journal of biochemistry》1981,13(5):591-602
- 1.1. A choriolytic enzyme was isolated from the hatching medium of the pike, Esox lucius.
- 2.2. The enzyme is defined as hatching enzyme.
- 3.3. The molecular weight of the enzyme is 24,000.
- 4.4. The enzyme is a glycoprotein containing 2% carbohydrate.
- 5.5. Its isoelectric point is 6.5.
- 6.6. The pH optimum is around pH 8.
- 7.7. The enzyme molecule contains two disulfide bonds but no free cysteine.
- 8.8. Inhibitor studies and metal analysis show that the enzyme is a zinc-metalloprotease.
6.
《Ethology and sociobiology》1988,9(5):319-324
This paper comments on: Low, B. S., Alexander, R. D., and Noonan, K.M. Human hips, breast, and buttocks: Is fat deceptive? Ethology and Sociobiology 8: 249-247, 1987. In it I argue that:
- 1.1. Sexual selection has probably not been the most important selection pressure on
- 2.female human body shape.
- 3.2. Male humans in different cultures find different aspects of the female body attractive
- 4.and therefore are unlikely to have exerted consistent directional sexual selection on
- 5.the female body.
- 6.3. Breast size is not correlated with lactation success.
- 7.4. Visible hip width is not correlated with parturition success.
- 8.5. Women would lower their fitness if they tried to deceive men about their internal
- 9.pelvic dimensions.
- 10.6. There are many alternative hypothesis to explain the existence of fat onwomen's
- 11.breast, hips, and buttocks.
7.
《The International journal of biochemistry》1993,25(6):885-890
- 1.1. Among the digestive enzymes synthesized by pancreas, lipase is the principle lipolytic enzyme which hydrolyses dietary glycerides.
- 2.2. For its action it requires a coenzyme, colipase.
- 3.3. The molecular mechanisms of the interaction of these two are not fully understood.
- 4.4. Further, molecular events that regulate and influence lipid absorption are ill denned.
- 5.5. The rabbit is the conventional animal model for the study of lipid absorption. We have undertaken the molecular cloning, and characterization of rabbit pancreatic colipase, the coenzyme for pancreatic lipase.
- 6.6. Colipase has been cloned from a gt 11 library of an adult rabbit pancreatic cDNA by probing with an oligonucleotide derived from human colipase sequence.
- 7.7. The total reading frame consists of 321 nucleotides coding for 90 amino acids of the functional protein and 17 nucleotides of the leader peptide.
- 8.8. Northern blot analysis revealed a distinct band around 0.5kb. Comparison with other species revealed an over all homology of 75% at the nucleotide level.
- 9.9. At the amino acid level highest conservation is observed at the lipase-binding region (AA 53–73).
- 10.10. Rabbit enzyme also retained the N-terminal pentapeptide of it preform.
- 11.11. The regions of homology and conservation may aid to define the sites of interaction of colipase with lipase.
8.
《The International journal of biochemistry》1984,16(12):1237-1243
- 1.1. Human placental alkaline phosphatase was inactivated with tetranitromethane in a biphasic process.
- 2.2. Spectral and amino acid analysis demonstrated that the inactivation was due to the conversion of tyrosine residues to 3-nitrotyrosine.
- 3.3. The inactivation process showed saturation kinetics.
- 4.4. Protection of the enzyme against tetranitromethane inactivation was afforded by inorganic phosphate.
- 5.5. The binding affinity between the modified enzyme and inorganic phosphate was decreased.
- 6.6. Our results suggest the involvement of tyrosyl residues in the locus of phosphoryl site of the phosphorylated enzyme forms.
9.
《Comparative biochemistry and physiology. A, Comparative physiology》1981,68(3):355-360
- 1.1. Cat plasma prothrombin and partial thromboplastin times are faster than human. Thromboplastin generation tests are very similar.
- 2.2. Factors VIII and V assay 24 and 13 times the human standard. Cat factors VII, X. IX, XI and XII assayed at 2.5 to 4 times human. Factors I, II and XIII fell within the human range and Fletcher was extremely low.
- 3.3. One cat lacked factor XII and showed a prolonged APTT and clotting time.
- 4.4. Cat profibrinolysin was activated by streptokinase but not by urokinase.
- 5.5. Cat platelets aggregated with the usual human aggregation agents with the exception of thrombin and ristocetin.
- 6.6. Cat erythrocytes were smaller and more numerous than human.
- 7.7. Leukocyte counts were quite variable.
- 8.8. Serum protein electrophoretic patterns differed from human in the greater migration of albumin and the presence of numerous unidentified bands.
- 9.9. Biochemical tests showed high sodium and chloride values.
10.
《The International journal of biochemistry》1993,25(2):157-161
- 1.1. To understand the physiological roles of the 90-kDa stress protein (HSP90), we investigated the heparin- and antibody-binding domains of the protein.
- 2.2. For heparin-binding sites, HSP90 was digested completely with trypsin, and the digests were applied to a heparin-Sepharose column and eluted with 1.0 M NaCl, followed by 8.0 M urea.
- 3.3. Each elutant was purified by a reverse-phase C18 column.
- 4.4. Two peptides from the NaCl-eluted fraction and no peptide from the urea-eluted fraction were purified.
- 5.5. The purified peptides were sequenced by an automated peptide sequencer.
- 6.6. One of the heparin-binding sites was present between Leu-362 and Arg-365; another was present between Leu-645 and Lys-648.
- 7.7. These two peptides were basic and considerably hydrophilic.
- 8.8. For antibody-binding sites, HSP90 was mildly digested with trypsin, electrophoresed on SDS-polyacrylamide gels and transferred to PVDF membranes.
- 9.9. The four bound of the trypsin fragments could be sequenced with a peptide sequencer.
- 10.10. There was only one antibody-binding peptide, 38 kDa, starting from Pro-2. The others showed no cross-reactivity with the antibody and started from Leu-283.
- 11.11. Therefore, the epitopes of HSP90 are present between Pro-2 and Leu-282.
- 12.12. The heparin-binding sites are present from the middle region of the HSP90 molecule, and the antigen sites are at the N-terminal domain.
11.
《The International journal of biochemistry》1985,17(5):589-595
- 1.1. A quick and simple procedure is described for purifying kallikrein from human whole saliva. The enzyme has been purified about 2700-fold with a yield of approx. 30%.
- 2.2. The procedure is based on the immediate fractionation of saliva by ion exchange chromatography. This is followed by a combination of affinity and high performance liquid chromatography.
- 3.3. The results indicate that another protein component binds to the enzyme at pH 8.0.
- 4.4. The homogeneity of the enzyme has been demonstrated by gel electrophoresis in the absence as well as in the presence of sodium dodecylsulfate.
- 5.5. A mol. wt of 40,100±1800 has been calculated from gel electrophores is experiments.
- 6.6. Sedimentation equilibrium in an analytical ultracentrifuge gave a mol. wt of 39,700.
- 7.7. The amino acid composition has been determined and it confirms that the enzyme has a low isoelectric point.
- 8.8. The presence of tryptophan has been demonstrated by absorption and fluorescence spectroscopy.
12.
《Comparative biochemistry and physiology. A, Comparative physiology》1991,98(2):407-412
- 1.1. Synaptic short-term depression could be transferred into long term depression by repetition of series of stimuli.
- 2.2. The transition from short-term depression to long-term depression was blocked by puromycin.
- 3.3. The majority of the transition took place during resting periods between stimulus series.
- 4.4. The initiation of the transition process was 83% completed after 5 min of stimulation.
- 5.5. Short- and long-term depression were quantitatively separated into their two serial sites of origin: afferent axons and synaptic terminals.
- 6.6. Long sequences evoked periods with increased and variable EPSPs not conforming to depression.
13.
《The International journal of biochemistry》1993,25(8):1195-1202
- 1.1. Three calcium-binding proteins have been purified from Ehrlich ascites tumor cells.
- 2.2. They were identified by amino acid sequence analysis on selected fragments obtained by tryptic digestion.
- 3.3. The proteins belong to the annexin family and were identified as annexins II, III and V.
- 4.4. Antibodies raised against the proteins were used to examine for their presence in a number of murine tissues.
- 5.5. The occurrence was found to be in reasonable accordance with earlier reports.
14.
《Comparative biochemistry and physiology. C: Comparative pharmacology》1988,89(2):479-482
- 1.1. The effects of injected catecholamines and their analogues on odour learning in honey bees is described.
- 2.2. Dopamine blocks the retrieval of a learned odour signal with a specific time course and does not block the storage of this signal.
- 3.3. Noradrenaline blocks retrieval and storage of a conditioned odour signal.
- 4.4. Amphetamine shows the same effects as noradrenaline.
- 5.5. Haloperidol has no affect on memory retrieval or storage.
15.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1986,83(2):347-353
- 1.1. Two vitellogenins and chromoprotein 2 are selectively accumulated by the oocyte and cannot be detected either in follicle cells or in the germarium.
- 2.2. At the start of their accumulation in terminal oocytes they are asymmetrically distributed.
- 3.3. Endocytosis of vitellogenin 1 starts somewhat later than the uptake of vitellogenin 2 and chromoprotein 2.
- 4.4. In follicle cells of young follicles, a protein (DLP), immunologically related to diapause protein 1, is highly concentrated.
- 5.5. During vitellogenesis DLP is sequestered by the oocytes.
- 6.6. The protein rich globules in terminal oocytes contain the vitellins as well as chromoprotein 2 and DLP.
16.
《The International journal of biochemistry》1991,23(3):293-299
- 1.1. In a continuing investigation of phycocyanin-membrane surface interaction, fluorescence quenching experiments were performed with a mixture of two populations of fluorescence probe-encapsulated phospholipid bilayer vesicles in the presence and absence of phycocyanin.
- 2.2. These membrane vesicles were prepared with 1,2-dimyristoyl phosphatidylcholine (DMPC), cholesterol and a probe molecule.
- 3.3. A fluorophore was encapsulated in one population of membrane vesicles, while a quencher was encapsulated in another population of membrane vesicles.
- 4.4. The result was compared with those of experiments in the presence of other biomolecules, including albumin, cytochrome c, hemoglobin, myoglobin or RNA.
- 5.5. Interestingly, a one-third reduction of the fluorescence intensity was observed in the mixture of these two populations of membrane vesicles in phycocyanin's presence.
- 6.6. In contrast, the other biomolecules caused no significant reduction in the fluorescence intensity.
- 7.7. These findings were evidence of a phycocyanin-induced membrane perturbation.
- 8.8. This was further demonstrated by a phycocyanin-induced change in the thermotropic behavior of DMPC vesicles, as measured by differential scanning microcalorimetry.
- 9.9. Such a unique property of phycocyanin is believed to be associated with its known membrane surface-interacting character.
- 10.10. A possible phycocyanin-modulated membrane-membrane interaction was discussed.
17.
- 1.1. From the muscle of 20 species of fresh-water fishes, l-histidine, carnosine, anserine, and balenine were analysed by high-performance liquid chromatography.
- 2.2. All cyprinoidei fishes contained significant amount of l-histidine and trace of dipeptides.
- 3.3. High concentration of anserine was found in salmonoidei fishes, irrespective of salmonidae and osmeridae.
- 4.4. Two species of anguilloidei contained large amount of carnosine, small of l-histidine, and determinable of anserine and balenine.
- 5.5. Only trace amounts of these compounds were found in percoidei fishes.
- 6.6. The levels of these compounds represented no large difference among species belonging to sub-order group as well as family.
18.
《The International journal of biochemistry》1993,25(9):1291-1301
- 1.1. Cytosolic and microsomal epoxide hydrolyzing enzymes of human skin and liver were compared and found to be different.
- 2.2. Epidermal and hepatic cytosolic epoxide hydrolases were different in terms of substrate selectivity, pI, inhibitor sensitivity and affinity Chromatographic properties.
- 3.3. Microsomal epoxide hydrolases had the same pIs but different substrate selectivities.
- 4.4. Cytosolic epoxide hydrolase from adults had higher specific activity than that from neonates or cultured epidermis, but lower activity than adult hepatic enzymes.
- 5.5. The sizes of cytosolic epoxide hydrolase from epidermis and liver were similar and lower than that from cultured fibroblasts.
- 6.6. Cytosolic epoxide hydrolase from all sources shared similar antigenic determinants.
19.
- 1.1. Brain trehalase specific activity and trehalosemia were measured during the end of the developmental life cycle in non-diapausing and diapausing insects.
- 2.2. During non-diapausing development, trehalosemia reached maximum values at the beginning of pupal life. Then a constant decrease was observed up to the end of adult life.
- 3.3. The specific activity of brain trehalase was maximum when the insects were in active feeding periods, minimum activity appearing during moulting phases.
- 4.4. During diapausing development, trehalosemia was very high at the beginning of pupal life, particularly when insects were exposed to wintering conditions.
- 5.5. When diapause was broken, trehalosemia fell, announcing adult emergence.
- 6.6. Brain trehalase activity showed the same qualitative variations as in non-diapausing larvae, but with rather lower values.
- 7.7. During pupal life, brain trehalase activity decreased markedly during the long period necessary to obtain diapause breakdown.
- 8.8. Wintering conditions allow a progressive increase of brain trehalase activity, which preceded the fall of trehalosemia.
20.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,101(4):963-967
- 1.1. Vitellogenin (VG) was isolated and purified from the hemolymph of female American cockroaches.
- 2.2. The purification method used in this study comprises two steps: the first step is based on the method originally developed for purifying lipophorin from hemolymph, and the second step is the separation of VG from lipophorin by a KBr density gradient ultracentrifugation.
- 3.3. The purified VG was characterized according to molecular weight, substructure, shape and size, and lipid composition.
- 4.4. The VG molecule is almost globular in shape with the diameter of about 15.5 nm and is indistinguishable from lipophorin in shape and size.
- 5.5. The native molecular weight determined by light scattering method was 560 kDa.
- 6.6. The VG consists of four subunits with molecular weights of approximately 102, 81, 49 and 40 kDa, respectively.
- 7.7. VG is a lipoprotein and comprises 92% protein and 8% lipid.
- 8.8. Major lipid components were found to be diacylglycerol (25%) and phospholipids (71%).