An intergeneric hybrid,Campostoma anomalum × Rhinichthys atratulus,from the Youghiogheny River drainage,West Virginia

The first record of a natural intergeneric hybrid of Campostoma anomalum × Rhinichthys atratulus is described from the Youghiogheny River drainage. Morphometric and meristic characters of the parents and hybrid were used to calculate a mean hybrid index value of 49.8. Dentition of the hybrid was intermediate as was intestinal coiling. In addition to the hybrid index, C. anomalum, R. atratulus and the hybrid were examined electrophoretically. Of the 32 enzymatic loci resolved, six (ADH-A, EST-B, LDH-A, PGM-A, SDH-B and SOD-C) showed hybrid inheritance patterns identical to the enzyme pattern obtained by mixing tissue extracts composed of the two parental species.     

Precision dating of Cook’s Mill,a Civil War era structure in West Virginia

Dendroarchaeology can provide critical understanding of a structure built during key historic periods, such as the American Civil War (1861–1865), when historical documentation is likely to be sparse or incomplete. Cook’s Mill is located in Greenville, West Virginia and extensive information derived from deeds, court records, wills, and oral history places the present mill’s original construction in 1857. The American Civil War began shortly after its construction and military conflict in the area led to the burning of several key structures, one of which was an unknown mill in Greenville (formerly Centerville). Written history suggests the mill is original and survived the American Civil War, however we used dendroarchaeology to confirm its precise date of construction. We collected 46 samples from the mill and 6 cross sections from a nearby exhumed bridge for dendrochronological dating. The mill was constructed with white oak (Quercus alba) and tulip poplar (Liriodendron tulipifera) logs and the bridge samples were white oak. We visually and statistically crossdated 32 white oak samples from the mill and bridge by comparing them to a local chronology developed for this study and two regional oak chronologies from the International Tree-Ring Data Bank. Based on terminal ring attributes and cutting date years we were able to provide a suggested construction date of the spring or early summer of 1868. This date suggests Cook’s Mill was the mill burned during military conflict in the area and that the current structure was subsequently rebuilt following the conclusion of the war.     

Aflatoxin contamination of Virginia peanuts for the crop-years 1982–1986

To establish those environmental conditions which promote the growth of aflatoxin (AFT)-producing Aspergillus spp. on peanuts, a four-year (1982–1986) investigation was undertaken to examine possible relationships between air temperature (AT), precipitation (P), and AFT contamination of stored nuts. The mean percentages of nuts that possessed various AFT levels for the years 1982–1986 (June–July) ranged from 74·2 to 88·0 for 0–4 ppb, 6·3 to 14·9 for 5–15 ppb, 2·4 to 5·9 for 16–25 ppb, 2·3 to 6·4 for 26–100 ppb, and 0 to 4·7 for > 100 ppb. The mean percentages for the years which exceeded USDA/FDA regulations were 7·1 (1982–1983), 7·6 (1983–1984), 11·6 (1984–1985), and 17·0 (1985–1986). Examination of the mean percentage > 15 ppb for each month during these four years revealed that the following months fell within that range; Septtember, November, December, January, February, and May (1982–1983); July, October, April, and June (1983–1984); August, and June, (1984–1985); and July, April, and May (1985–1986). Comparisons of pooled-AFT levels, rainfall, and temperature over four years suggested a ‘better fit’ between mean monthly P and mean percentage AFTs > 15 ppb, than between the latter and mean monthly AT. However, application of a predictor equation suggested a correlation between AFT levels and monthly AT.     

Arbuscular mycorrhizal fungal diversity in tropical sand dune and restinga at Peró Beach in Rio de Janeiro state,Brazil

The diversity of arbuscular mycorrhizal fungi (sub-phylum Glomeromycotina) in two contrasting coastal ecosystems (dunes and restinga) at Peró Beach, Rio de Janeiro state, Brazil was evaluated at the end of the rainy (May) and dry (November) seasons, in 2014. A total of 22 species belonging to ten genera and five families were recorded. Glomus macrocarpum and Rhizoglomus microaggregatum had large biomass and frequencies of glomerospores at both sites. Glomerospore abundance was higher in the dry season in dunes and in the rainy season in restinga. Redundancy analysis revealed significant relationships between AMF-physical and chemical soil parameters. Shannon's index and Pielou's evenness indicated greater AMF diversity in dunes than restinga, despite the lower number of glomerospores in dunes. These results highlight the importance of knowing the AMF communities in different coastal ecosystems, especially in dunes, which due to the low vegetation cover are most frequently impacted by anthropic actions.     

Analyzing Beach Recreationists’ Preferences for the Reduction of Jellyfish Blooms: Economic Results from a Stated-Choice Experiment in Catalonia,Spain

Jellyfish outbreaks and their consequences appear to be on the increase around the world, and are becoming particularly relevant in the Mediterranean. No previous studies have quantified tourism losses caused by jellyfish outbreaks. We used a stated-choice questionnaire and a Random Utility Model to estimate the amount of time respondents would be willing to add to their journey, in terms of reported extra travel time, in order to reduce the risk of encountering jellyfish blooms in the Catalan coast. The estimation results indicated that the respondents were willing to spend on average an additional 23.8% of their travel time to enjoy beach recreation in areas with a lower risk of jellyfish blooms. Using as a reference the opportunity cost of time, we found that the subsample of individuals who made a trade-off between the disutility generated by travelling longer in order to lower the risk of jellyfish blooms, and the utility gained from reducing this risk, are willing to pay on average €3.20 per beach visit. This estimate, combined with the respondents’ mean income, yielded annual economic gains associated with reduction of jellyfish blooms on the Catalan coast around €422.57 million, or about 11.95% of the tourism expenditures in 2012. From a policy-making perspective, this study confirms the importance of the economic impacts of jellyfish blooms and the need for mitigation strategies. In particular, providing daily information using social media applications or other technical devices may reduce these social costs. The current lack of knowledge about jellyfish suggests that providing this information to beach recreationists may be a substantially effective policy instrument for minimising the impact of jellyfish blooms.     

Outbreak of Hepatitis B Virus Infections Associated with Assisted Monitoring of Blood Glucose in an Assisted Living Facility–Virginia, 2010

Introduction

In January 2010, the Virginia Department of Health received reports of 2 hepatitis B virus (HBV) infections (1 acute, 1 chronic) among residents of a single assisted living facility (ALF). Both infected residents had diabetes and received assisted monitoring of blood glucose (AMBG) at the facility. An investigation was initiated in response.

Objective

To determine the extent and mechanism of HBV transmission among ALF residents.

Design

Retrospective cohort study.

Setting

An ALF that primarily housed residents with neuropsychiatric disorders in 2 adjacent buildings in Virginia.

Participants

Residents of the facility as of March 2010.

Measurements

HBV serologic testing, relevant medical history, and HBV genome sequences. Risk ratios (RR) and 95% confidence intervals (CIs) were used to identify risk factors for HBV infection.

Results

HBV serologic status was determined for 126 (91%) of 139 residents. Among 88 susceptible residents, 14 became acutely infected (attack rate, 16%), and 74 remained uninfected. Acute HBV infection developed among 12 (92%) of 13 residents who received AMBG, compared with 2 (3%) of 75 residents who did not (RR  = 35; 95% CI, 8.7, 137). Identified infection control breaches during AMBG included shared use of fingerstick devices for multiple residents. HBV genome sequencing demonstrated 2 building-specific phylogenetic infection clusters, each having 99.8–100% sequence identity.

Limitations

Transfer of residents out of the facility prior to our investigation might have contributed to an underestimate of cases. Resident interviews provided insufficient information to fully assess behavioral risk factors for HBV infection.

Conclusions

Failure to adhere to safe practices during AMBG resulted in a large HBV outbreak. Protection of a growing and vulnerable ALF population requires improved training of staff and routine facility licensing inspections that scrutinize infection control practices.     

Fruits of Wetherellia and Palaeowetherellia (?Euphorbiaceae) from Eocene sediments in Virginia and Maryland

The Eocene sediments of southern Maryland and adjacent Virginia have yielded multilocular fruits assignable to the extinct generaWetherellia Bowerbank andPalaeowetherellia Chandler. This is the first report of their co-occurrence in one deposit.Wetherellia has previously been reported from the Eocene of southern England and West Germany (two species), whilePalaeowetherellia has been reported from the Eocene and possibly latest Cretaceous of Egypt (one species). APalaeowetherellia-like fruit has been reported from the Eocene of southeastern North America.Wetherellia marylandica comb. nov. andPalaeowetherellia species are described; new information is provided for the previously established speciesWetherellia variabilis andW. dixonii. The familial assignment of both genera is reviewed.Palaeowetherellia is most similar to fruits of the tribe Hippomaneae of the Euphorbiaceae, whileWetherellia finds counterparts in the Euphorbiaceae and the Meliaceae. While the exact affinities ofWetherellia are difficult to resolve through comparison to modern fruits, the similarity ofWetherellia andPalaeowetherellia might suggest that the former is more likely euphorbiaceous than meliaceous. The discovery of these New World members of both genera reinforces the importance of floristic exchange across the North Atlantic in earliest Tertiary time.     

Seasonal variation of energy metabolism in ghost crab Ocypode quadrata at Siriú Beach (Brazil)

The aim of this study was to analyze the seasonal variations of carbohydrate and lipid metabolism of the ghost crab Ocypode quadrata (Fabricius, 1787) on a sandy beach in the southern region of Brazil. Crabs and hemolymph samples were collected monthly in the field. Hepatopancreas, gills, gonads and claw muscles were used for glycogen determination. In males, blood glucose levels increased in the summer and in the winter. The glycogen values increased significantly in the hepatopancreas in the winter, but remained constant in the muscle, gonads and gills. In females, hemolymph glucose levels, glycogen values in the hepatopancreas and in the gills remained constant throughout the year; however, muscular glycogen increased in the spring and gonad glycogen decreased in the summer. Hemolymph triglyceride levels of males and females and total cholesterol of males decreased significantly in the spring. In females, a significant increase of total cholesterol levels was found in the winter. The findings suggest that in O. quadrata lipids seem to be an important reserve of energy used during reproduction, both in males and females, while glycogen may be used during periods of intense activity or fasting.     

Seasonal changes in the physiology of male Virginia opossums (Didelphis virginiana): signs of the Dasyurid semelparity syndrome?

Semelparity, which is multiplying once in a lifetime, is a rare reproductive strategy among mammals. Several species of the marsupial family Dasyuridae experience 100% male mortality following an intense mating period. We investigated seasonal physiological changes that may be associated with early mortality in the male Virginia opossum (Didelphis virginiana; Didelphidae) and compared these changes with those of semelparous, male dasyurids. Free-ranging male Virginia opossums (n=36) were collected during 2001 at the Oklahoma State University Cross Timbers Experimental Range. Seasonal data were collected on hematological, morphological, and helminth parameters of these individuals. We used one-way ANOVA to determine whether there were seasonal differences among means for each parameter. It appeared that male Virginia opossums experienced some physiological changes similar to those of male dasyurids exhibiting semelparity. All males collected in summer (August) were juveniles of the year. Lack of adult males in August suggests high mortality of this cohort during the breeding season. Opossum characteristics exhibiting the dasyurid semelparity syndrome included packed cell volume, adrenal mass, and helminth numbers. Minor lymphocytopenia, neutrophilia, and testosterone concentrations also were similar to semelparous dasyurids. However, a lack of change in serum cortisol concentration and body mass and dynamics in immunoglobulin protein, serum protein, and testes mass were not consistent with previous reports of semelparous dasyurid physiology. Evolutionary divergence and differences in breeding behavior between dasyurids and didelphids may be responsible for the lack of consistency between the taxa.     

Autophagosomes,phagosomes, autolysosomes,phagolysosomes, autophagolysosomes… Wait,I’m confused

When an autophagosome or an amphisome fuse with a lysosome, the resulting compartment is referred to as an autolysosome. Some people writing papers on the topic of autophagy use the terms “autolysosome” and “autophagolysosome” interchangeably. We contend that these words should be used to denote 2 different compartments, and that it is worthwhile maintaining this distinction—the autophagolysosome has a particular origin in the process of xenophagy that makes it distinct from an autolysosome.     

Autophagosomes,phagosomes, autolysosomes,phagolysosomes, autophagolysosomes… Wait,I’m confused

When an autophagosome or an amphisome fuse with a lysosome, the resulting compartment is referred to as an autolysosome. Some people writing papers on the topic of autophagy use the terms “autolysosome” and “autophagolysosome” interchangeably. We contend that these words should be used to denote 2 different compartments, and that it is worthwhile maintaining this distinction—the autophagolysosome has a particular origin in the process of xenophagy that makes it distinct from an autolysosome.