戴云山黄山松群落主要树种更新生态位研究

为探讨戴云山黄山松(Pinus taiwanensis)群落的分化与动态更新,采用改进的更新生态位宽度和更新生态位重叠公式研究黄山松群落主要树种的更新生态位。结果表明,不同物种对空间资源的利用存在差异,不同物种具有不同的更新生态位宽度,物种间的更新生态位重叠值也不同。在群落乔木层,黄山松的更新生态宽度最大,因此,黄山松在群落中占优势地位。黄山松与其它物种的更新生态位重叠值主要在0.1~0.39之间,表明黄山松与其它树种存在共用资源利用谱。黄山松与窄基红褐柃(Eurya rubiginosa)和短尾越桔(Vaccinium carlesii)的更新生态位重叠值较大,与这些物种的竞争和排斥作用明显,不利于黄山松幼苗和幼树更新,因此建议采用人工抚育措施并加强保护,提高黄山松幼苗和幼树的成活率,使群落处于稳定状态。     

戴云山国家级自然保护区黄山松群落类型与物种多样性分析

采用典型取样法对戴云山国家级自然保护区黄山松(Pinus taiwanensis Hayata)群落的类型和物种多样性进行了分析。以乔木层主要树种的重要值为参考指标,通过系统聚类分析,可将该黄山松群落分为8个群丛类型:黄山松+杉木-乌药-铁芒萁群丛(Assoc.P.taiwanensis+Cunninghamia lanceolata-Lindera aggregate-Dicranopterislinearis);黄山松+多脉青冈-细枝柃-里白群丛(Assoc.P.taiwanensis+Cyclobalanopsis multinervis-Eurya loquaiana-Hicriopteris glauca);黄山松+江南山柳-短尾越桔-铁芒萁群丛(Assoc.P.taiwanensis+Clethra cavaleriei-Vacciniumcarlesii-Dicranopteris linearis);黄山松+甜槠-肿节少穗竹-里白群丛(Assoc.P.taiwanensis+Castanopsis eyrei-Oligostachyum oedogonatum-Hicriopteris glauca);黄山松+吊钟花-短尾越桔-狗脊群丛(Assoc.P.taiwanensis+Enkianthus quinqueflorus-Vaccinium carlesii-Woodwardia japonica);黄山松+华丽杜鹃-短尾越桔-里白群丛(Assoc.P.taiwanensis+Rhododendron farrerae-Vaccinium carlesii-Hicriopteris glauca);黄山松+光叶水青冈-满山红-铁芒萁群丛(Assoc.P.taiwanensis+Fagus lucida-Rhododendron mariesii-Dicranopteris linearis);黄山松-满山红-平颖柳叶箬群丛(Assoc.P.taiwanensis-Rhododendron mariesii-Isachne truncata)。各群丛总体物种丰富度指数和多样性指数总体上呈随海拔升高而降低的趋势,总体均匀度指数Pielou指数和Alatalo指数波动不大。在群落垂直结构上,乔木层、灌木层和草本层物种丰富度指数、多样性指数和均匀度指数有差异;各群丛间乔木层和灌木层的物种丰富度指数、多样性指数和均匀度指数波动较大,草本层这3个指数波动较小且数值上小于前两者。分析结果显示:海拔是决定该黄山松群落多样性差异的主导因子。     

浙江省松阳县黄山松种群的年龄结构与分布格局

黄山松在我国东南部海拔700—2000m中山带广泛分布,是该地区森林群落中重要的组成成分。黄山松种群在森林群落演替中起着先锋种群的作用。但在裸岩陡坡或峰岭梁岗等特殊生境中,黄山松种群亦能形成稳定的群落。本文在种群的水平上,分析了浙江省松阳县关山源地区黄山松种群在不同演替阶段群落中和不同生境中的年龄结构与种群空间分布格局,并探讨了它们的动态规律及其与环境之间的相互关系。黄山松种群的研究不仅有助于探讨植物生态学研究中的一些理论问题,而且对经营管理和保护利用具有一定的实践意义。     

浙江省松阳县黄山松种群的密度与生物量动态

 本文研究了浙江省松阳县关山源地区黄山松种群的密度与生物量动态以及它们之间的相互关系。黄山松是该地区森林演替中的先锋种群之一。在演替过程中,黄山松种群的动态可分成三个阶段。大约在黄山松种群入侵次生裸地的最初10年期间,种群的密度和生物量迅速增长(阶段Ⅰ)。此后,种群密度达到饱和,由于自疏作用出现以及其他阔叶树种的入侵,种群密度开始急剧下降,个体平均重量和种群生物量迅速增长(阶段Ⅱ),–3/2自疏定律适用于种群动态的此阶段。随着阔叶树种进入林冠层,虽然个体平均重量仍缓慢增长,黄山松种群的密度和种群生物量逐渐下降直至退出群落(阶段Ⅲ)。但在一些特殊生境中(如裸岩陡坡或山脊),黄山松种群可形成稳定的地形顶极群落,其种群密度、个体平均重量和种群生物量可长期维持相对稳定的状态。     

Biodiversity of Mimosa pudica rhizobial symbionts (Cupriavidus taiwanensis, Rhizobium mesoamericanum) in New Caledonia and their adaptation to heavy metal-rich soils

Rhizobia are soil bacteria able to develop a nitrogen-fixing symbiosis with legumes. They are taxonomically spread among the alpha and beta subclasses of the Proteobacteria. Mimosa pudica, a tropical invasive weed, has been found to have an affinity for beta-rhizobia, including species within the Burkholderia and Cupriavidus genera. In this study, we describe the diversity of M.?pudica symbionts in the island of New Caledonia, which is characterized by soils with high heavy metal content, especially of Ni. By using a plant-trapping approach on four soils, we isolated 96 strains, the great majority of which belonged to the species Cupriavidus taiwanensis (16S rRNA and recA gene phylogenies). A few Rhizobium strains in the newly described species Rhizobium mesoamericanum were also isolated. The housekeeping and nod gene phylogenies supported the hypothesis of the arrival of the C.?taiwanensis and R.?mesoamericanum strains together with their host at the time of the introduction of M.?pudica in New Caledonia (NC) for its use as a fodder. The C.?taiwanensis strains exhibited various tolerances to Ni, Zn and Cr, suggesting their adaptation to the specific environments in NC. Specific metal tolerance marker genes were found in the genomes of these symbionts, and their origin was investigated by phylogenetic analyses.     

天然黄山松种群格局的分形特征——计盒维数与信息维数

采用计盒维数和信息维数对屏南和寿宁不同群落的天然黄山松(Pinus taiwanensis)种群格局的分形特征进行比较分析.结果表明,天然黄山松种群格局具有分形特征,其计盒维数值在1.299 8～1.862 6之间,不同群落的大小次序为Q3>Q1>Q2>Q4>Q7>Q8>Q5>Q6;信息维数值在1.205 7～1.863 7之间,大小次序是Q3>Q1>Q2>Q4>Q8>Q7>Q5>Q6,屏南天然黄山松近纯林黄山松种群的计盒维数和信息维数均高于寿宁混交林,计盒维数定量描述种群占据水平空间的能力和程度,信息维数揭示种群格局强度的尺度变化及个体分布的非均匀程度,分形维数值的高低与群落环境、种群密度、种群在群落中的优势地位、个体的聚集程度及幼树个体数量等相关.黄山松种群格局分形维数随海拔呈现上下波动变化,1 250～1 270 m是更适生的海拔范围.此外,黄山松种群格局的分形特征存在一定的尺度范围,其拐点尺度是分形范围的下限尺度.     

Susceptibility of Aedes aegypti and Aedes albopictus larvae to Ascogregarina culicis and Ascogregarina taiwanensis (Apicomplexa: Lecudinidae) from Florida

The susceptibility of Aedes aegypti to Ascogregarina culicis and Aedes albopictus to Ascogregarina taiwanensis was examined with mosquito and parasite strains from Tampa, FL. When each host was bioassayed with its natural gregarine, the infection intensity indicated that Ae. aegypti was 59% more susceptible to A. culicis (87 gamonts/larva) than Ae. albopictus to A. taiwanensis (47 gamonts/larva). Infections in single and mixed host populations exposed to 100 oocysts/larva of one and both parasites demonstrated that Ae. aegypti harbors higher A. culicis gamont loads than Ae. albopictus of A. taiwanensis. In dual gregarine exposures of single host populations, the A. culicis infection intensity in Ae. aegypti was reduced by approximately 50%. A. taiwanensis exhibited the same capability of infecting Ae. albopictus in single and dual exposures. In mixed host populations there were no cross infections, but A. taiwanensis in Ae. albopictus produced an infection intensity of approximately 70% lower than that of A. culicis in Ae. aegypti.     

中国辽秃蝗属的分类研究及二新种记述（直翅目， 蝗总科， 斑腿蝗科， 秃蝗亚科）（英文）

记述了我国辽秃蝗属Liaopodisma 3个种, 包括2新种： 台中辽秃蝗Liaopodisma taichungensis sp. nov.和台湾辽秃蝗Liaopodisma taiwanensis sp. nov.。台中辽秃蝗L. taichungensis sp. nov.同千山辽秃蝗L. qianshanensis Zheng, 1990近似, 不同之处为黑色眼后带不明显, 前胸背板中隆线几乎不见, 雄性前翅长为宽的1.8倍, 雄性中胸腹板中隔向后渐宽大, 雄性后足胫节外侧具10刺。台湾辽秃蝗L. taiwanensis sp. nov.的后足股节下侧红色, 前翅中部不加宽且前、 后缘几乎平行, 可与本属其余2已知种区别。并附辽秃蝗属种检索表。     

基于戴云山固定样地黄山松群落物种组成与结构研究

黄山松是亚热带中部山区主要建群种,研究其物种组成及群落结构对探讨中亚热带植被演替规律具有重要意义。戴云山自然保护区分布有大面积黄山松群落,该研究采用网格布点法,建立86块(25.82 m×25.82 m)共5.7 hm~2固定样地,对样地内黄山松群落的物种组成特征、分布区类型、胸径和树高结构、空间分布格局4个方面特征进行了探讨。结果表明:(1)样地内共出现木本植物32 603株219种,隶属于59科108属。乔灌木优势种有黄山松、马尾松、木荷、肿节少穗竹、岩柃、窄基红褐柃等,其中稀有种和偶见种分别占总物种数的33.79%、25.75%和45.21%、24.66%。(2)在区系分布类型上,热带分布类型的科属多于温带分布类型的科属,热带分布类型中又以泛热带分布类型,温带分布类型以北温带占据最大比例。(3)样地内优势种的胸径分布呈倒"J"型,表明更新状态良好。垂直结构上,各优势种树高为倒"J"型或近似倒"J"型。黄山松和其他优势乔木大部分树高为4~10 m,灌木除短尾越桔0~1 m个体数最多外,其他优势灌木1~2 m个体数最多。(4)经计算,样地内乔灌木各优势种均呈聚集分布,聚集程度有所差异,乔木层黄山松最低,灌木层短尾越桔最低。该研究结果表明戴云山保护区黄山松群落物种组成丰富、更新良好,反映了亚热带高山针叶林的典型特征,其空间分布格局可能与取样方式等因素有关。     

Laboratory susceptibility of Wyeomyia smithii (Diptera: Culicidae) to Ascogregarina taiwanensis (Apicomplexa: Lecudinidae)

Gregarines in the genus Ascogregarina are not known to develop in sabethine mosquitoes, but we successfully infected larvae of Wyeomyia smithii with Ascogregarina taiwanensis in the laboratory. Ascogregarina taiwanensis is a natural parasite of the exotic Asian tiger mosquito, Aedes albopictus. Only 18% to 70% of the W. smithii larvae had visible trophozoites, with a range of 1-92 per larva. Trophozoites persisted in the midgut for more than 37 d, and one adult female W. smithii had gametocysts in its Malphigian tubules, which indicated that A. taiwanensis might fully develop in W. smithii. After 50 d, gregarines were not found in W. smithii larvae.     

天柱山黄山松种内与种间竞争的研究

采用改进的竞争指数模型研究安徽天柱山黄山松的种内和种间竞争强度。结果发现:(1)随对象木胸径的增大,黄山松种群因自然稀疏过程中密度调节作用,植株距离增加,种内竞争强度降低;(2)黄山松群落内其它物种虽然较多,但个体普遍较小,结果种间竞争相对较弱,种内与种间竞争关系顺序为:黄山松—黄山松>杉木—黄山松>枹栎—黄山松>其它树种—黄山松;(3)竞争强度和对象木胸径的关系服从幂函数关系(CI=AD-B),当黄山松胸径达到20cm以上时,竞争强度变化不大,所得的预测模型能很好地预测黄山松种内和种间的竞争强度;(4)改进的竞争指数模型能很好地度量黄山松的种内和种间竞争强度,改进的确定邻体范围的方法能有效地确定黄山松的竞争范围。     

麻城黄山松天然林直径分布

为研究黄山松天然次生林直径分布特征,以麻城市黄山松天然次生林为研究对象,采用标准样地调查,计算林分直径的偏度、峰度,林分直径分布的Shannon-Weiner和Simpson指数,运用负指数分布、normal分布、lognormal分布、logistic分布和Weibull分布等5种概率密度函数对黄山松天然次生林林分直...     

Differential identification of Ascogregarina species (Apicomplexa: Lecudinidae) in Aedes aegypti and Aedes albopictus (Diptera: Culicidae) by polymerase chain reaction

We report 2 polymerase chain reaction (PCR)-based methods for distinguishing morphologically similar gregarine species based on amplification of variable regions of the internal transcribed spacer region of ribosomal DNA. The gregarines we investigated were Ascogregarina barretti (Vavra), A. culicis (Ross), and A. taiwanensis (Lien and Levine), parasites of the mosquitoes Ochlerotatus triseriatus (Say), Aedes aegypti (Linnaeus), and Ae. albopictus (Skuse), respectively. These 3 important vector mosquitoes often utilize the same container habitats, where larval development and infection by the parasite occurs, leaving ample opportunity for cross-species gregarine infection. Because previous studies have shown that the parasites A. culicis and A. taiwanensis variably affect fitness in both normal and abnormal mosquito hosts, distinguishing parasite infection and species is important. The task is complicated by the fact that these 2 parasite species are virtually identical in morphology, whereas A. barretti is morphologically distinct. Of the 2 PCR-based assays reported here, the first provides a rapid, sensitive, and straight-forward means of general ascogregarine detection based on a single PCR amplification. The second method provides a means of differentiation between A. culicis and A. taiwanensis based on a species-specific PCR assay. Together, these assays allow whole mosquitoes to be tested for the presence of Ascogregarina species as well as identification of both A. culicis and A. taiwanensis singly or in dual infections.     

Distinguishing between two sympatric Acanthopagrus species from Dapeng Bay, Taiwan, using morphometric and genetic characters

Morphometric and genetic data were used to compare two sympatric and morphologically similar species, Acanthopagrus berda and Acanthopagrus taiwanensis , in Dapeng Bay, South-western Taiwan. A principle component analysis of morphological data indicated a distinction between the two species, with pectoral fin length and eye diameter accounting for 32·27% of the variation. Interspecific sequence divergence, based on mtDNA cytochrome b (0·118 ± 0·01), was larger than intraspecific divergences between haplotypes (0·007 for A. taiwanensis and 0·003 for A. berda ). Individuals of the two species clustered into different groups in three phylogenetic trees with 100% bootstrap support. The mean observed heterozygosity for eight microsatellite loci was 0·471 ± 0·202 for A. taiwanensis and 0·637 ± 0·145 for A. berda . Nei's unbiased measure of interspecific genetic distance ( D _S) was 1·334. F _ST (0·134) and R _ST (0·404) values indicated significant differentiation between species. An unrooted neighbour-joining tree was constructed by allele-sharing distances and the factorial correspondence analysis split all specimens into two distinct clusters. The results of morphometric, mtDNA and microsatellite analyses indicated the presence of two species, A. taiwanensis and A. berda .     

Cupriavidus taiwanensis bacteroids in Mimosa pudica Indeterminate nodules are not terminally differentiated

The beta-rhizobium Cupriavidus taiwanensis forms indeterminate nodules on Mimosa pudica. C. taiwanensis bacteroids resemble free-living bacteria in terms of genomic DNA content, cell size, membrane permeability, and viability, in contrast to bacteroids in indeterminate nodules of the galegoid clade. Bacteroid differentiation is thus unrelated to nodule ontogeny.     

Bacterial swimming, swarming and chemotactic response to heavy metal presence: which could be the influence on wastewater biotreatment efficiency?

Fixed-bed reactors are usually designed for wastewater biotreatments, where the biofilm establishment and maintenance play the most important roles. Biofilm development strictly relies on different types of bacterial motility: swimming, swarming, and chemotaxis, which can be altered by the microenvironment conditions. The aim of this work is to do an integrated study on the effects of Cu(II), Cd(II), Zn(II) and Cr(VI) on swimming, swarming and chemotaxis of Pseudomonas veronii 2E, Delftia acidovorans AR and Ralstonia taiwanensis M2 to improve biofilm development and maintenance for metal loaded wastewater biotreatment in fixed-bed bioreactors. Swimming, swarming and chemotactic response evaluation experiments were carried out at different metal concentrations. P. veronii 2E motility was not affected by metal presence, being this strain optimal for fixed-bed reactors. D. acidovorans AR swarming was inhibited by Cd and Zn. Although R. taiwanensis M2 showed high resistance to Cu, Cd, Cr and Zn, motility was definitively altered, so further studies on R. taiwanensis M2 resistance mechanisms would be particularly interesting.     

Additional Records of Digenetic Trematodes of Birds from Taiwan

43 species of digenetic trematodes of birds are reported from Taiwan. One new family, one new subfamily, two new genera, and nine new species are described: Taiwantrematidae fam. n. Taiwantrema arborophilae gen. et sp. n. from Arborophila crudigularis; Cyclocoelidae, Morishitium taiwanense sp.n. from A. crudigularis; Eucotylidae, Tanaisia (Tamerlania) taiwanensis sp. n. from A. crudigularis; Brachylaimidae, Leucochloridium taiwanense sp. n. from A. crudigularis; Echinostomatidae, Echinostoma taiwanense sp. n. from Zosterops japonica simplex; Dicrocoeliidae, Concinnum taiwanense sp. n. from Pycnonotus taivanus; Lecithodendriidae, Pseudocryptotropa formosae sp. n. from Pycnonotus sinensis formosae; Microphallidae, Promicrophallinae subfam. n. Promicrophallus taiwanensis gen. et sp. n. from A. crudigularis; Opisthorchiidae, Metametorchis taiwanensis sp. n. from the domestic duck.     

Phylogeny based on 16S rDNA and nifH sequences of Ralstonia taiwanensis strains isolated from nitrogen-fixing nodules of Mimosa pudica, in India

Bacterial symbionts present in the indeterminate-type nitrogen (N)-fixing nodules of Mimosa pudica grown in North and South India showed maximum similarity to Ralstonia taiwanensis on the basis of carbon-source utilization patterns and 16S rDNA sequence. Isolates from the nodules of M. pudica from North India and South India showed identical ARDRA (Amplified Ribosomal DNA Restriction Analysis) patterns with Sau3AI and RsaI, but AluI revealed dimorphy between the North Indian and South Indian isolates. Alignment of 16S rDNA sequences revealed similarity of North Indian isolates with an R. taiwanensis strain isolated from M. pudica in Taiwan, whereas South Indian isolates showed closer relatedness with the isolates from Mimosa diplotricha. Alignment of nifH sequences from both North Indian and South Indian isolates with that of the related isolates revealed their closer affinity to alpha-rhizobia, suggesting that nif genes in the beta-rhizobia might have been acquired from alpha-rhizobia via lateral transfer during co-occupancy of nodules by alpha-rhizobia and progenitors of R. taiwanensis, members of the beta-subclass of Proteobacteria. Immunological cross-reaction of the bacteroid preparation of M. pudica nodules showed strong a positive signal with anti-dinitrogenase reductase antibody, whereas a weak positive cross-reaction was observed with free-living R. taiwanensis grown microaerobically in minimal medium with and without NH4Cl. In spite of the expression of dinitrogenase reductase under free-living conditions, acetylene reduction was not observed under N-free conditions even after prolonged incubation.     

Desiccation resistance of two semiterrestrial isopods, Ligia exotica and Ligia taiwanensis (Crustacea) in Taiwan

The ability to resist desiccation stress was examined in two semiterrestrial Ligia species, Ligia exotica Roux and L. taiwanensis Lee, in Taiwan, under a certain desiccation condition. L. exotica exhibited the longer survival time, lower weight-specific rates of water loss, and the slightly higher ability of tolerance to water loss, compared to L. taiwanensis. In each species, the animal size displays a positive correlation to the survival time and total ability to resist desiccation, yet this size effects on the weight-specific water loss rate is negative. Neither water content nor maximum tolerance to water loss shows the association with the animal size in both species. The path ways and magnitudes of the interactions between these traits of desiccation resistance are analyzed and diagrammed using a stepwise regression model. In this model, the body sizes of animal can explain the most part of the variations in the survival time. The body size has a direct effect and an indirect effect, through the effect on water loss rate, on the time that the experimental animals can survival under this desiccated condition. These results suggest that L. exotica attains larger size than does L. taiwanensis, a lower transpiration rate and, consequently, a greater ability in desiccation resistance. The performances of these interactions in the desiccated resistance are more advantageous for L. exotica to migrate and colonize in variable land habitats within a certain limit, and as a result that L. exotica shows a broader distribution pattern than did L. taiwanensis in Taiwan.     

黄山地区松树林外生菌根菌资源及生态分布

1998～2003年,对黄山地区松树林下外生菌根菌资源及生态分布进行了调查,鉴定出外生菌根菌43种,隶属于10科17属.其中马尾松林下有43种、黄山松林下有12种.马尾松幼林下的外生菌根菌种类单一,彩色豆马勃、硬皮马勃类为优势菌种;马尾松成熟林下外生菌根菌种类丰富,其优势种类为红菇科、鹅膏菌科、牛肝菌科和鸡油菌科的一些种类.并分析了树种植被对外生菌根菌分布的影响,菌根真菌的分布与温度、湿度及土壤条件的关系.