Meeting report of the OECD conference on “Genome Editing: Applications in Agriculture—Implications for Health,Environment and Regulation”

Transgenic Research - The “OECD Conference on Genome Editing: Applications in Agriculture—Implications for Health, Environment and Regulation” was held on the 28–29 June...     

Restauro-G： A Rapid Genome Re-Annotation System for Comparative Genomics

of complete genome sequences submitted directly from sequencing projects are diverse in terms of annotation strategies and update frequencies. These inconsistencies make comparative studies difficult. To allow rapid data preparation of a large number of complete genomes, automation and speed are important for genome re-annotation. Here we introduce an open-source rapid genome re-annotation software system, Restauro-G, specialized for bacterial genomes. Restauro-G re-annotates a genome by similarity searches utilizing the BLASTLike Alignment Tool, referring to protein databases such as UniProt KB, NCBI nr, NCBI COGs, Pfam, and PSORTb. Re-annotation by Restauro-G achieved over 98% accuracy for most bacterial chromosomes in comparison with the original manually curated annotation of EMBL releases. Restauro-G was developed in the generic bioinformatics workbench G-language Genome Analysis Environment and is distributed at http：//restauro-g.iab.keio.ac.jp/ under the GNU General Public License.     

Society for Mycotoxin Research—News and announcements

Society for Mycotoxin Research – News and Announcements

Society for Mycotoxin Research—News and announcements     

Targeted Genome Editing by Recombinant Adeno-Associated Virus （rAAV） Vectors for Generating Genetically Modified Pigs

Recombinant adeno-associated virus(rAAV) vectors have been extensively used for experimental gene therapy of inherited human diseases.Several advantages,such as simple vector construction,high targeting frequency by homologous recombination,and applicability to many cell types,make rAAV an attractive approach for targeted genome editing.Combined with cloning by somatic cell nuclear transfer(SCNT),this technology has recently been successfully adapted to generate gene-targeted pigs as models for cystic fibrosis, hereditary tyrosinemia type 1,and breast cancer.This review summarizes the development of rAAV for targeted genome editing in mammalian cells and provides strategies for enhancing the rAAV-mediated targeting frequency by homologous recombination.We discuss current development and application of the rAAV vectors for targeted genome editing in porcine primary fibroblasts,which are subsequently used as donor cells for SCNT to generate cloned genetically designed pigs and provide positive perspectives for the generation of gene-targeted pigs with rAAV in the future.     

A Compact,High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing

1. Download high-res image (271KB)
2. Download full-size image

     

Lablab purpureus—A Crop Lost for Africa?

In recent years, so-called ‘lost crops’ have been appraised in a number of reviews, among them Lablab purpureus in the context of African vegetable species. This crop cannot truly be considered ‘lost’ because worldwide more than 150 common names are applied to it. Based on a comprehensive literature review, this paper aims to put forward four theses, (i) Lablab is one of the most diverse domesticated legume species and has multiple uses. Although its largest agro-morphological diversity occurs in South Asia, its origin appears to be Africa. (ii) Crop improvement in South Asia is based on limited genetic diversity. (iii) The restricted research and development performed in Africa focuses either on improving forage or soil properties mostly through one popular cultivar, Rongai, while the available diversity of lablab in Africa might be under threat of genetic erosion. (iv) Lablab is better adapted to drought than common beans (Phaseolus vulgaris) or cowpea (Vigna unguiculata), both of which have been preferred to lablab in African agricultural production systems. Lablab might offer comparable opportunities for African agriculture in the view of global change. Its wide potential for adaptation throughout eastern and southern Africa is shown with a GIS (geographic information systems) approach.     

Genomer — A Swiss Army Knife for Genome Scaffolding

The increasing accessibility and reduced costs of sequencing has made genome analysis accessible to more and more researchers. Yet there remains a steep learning curve in the subsequent computational steps required to process raw reads into a database-deposited genome sequence. Here we describe “Genomer,” a tool to simplify the manual tasks of finishing and uploading a genome sequence to a database. Genomer can format a genome scaffold into the common files required for submission to GenBank. This software also simplifies updating a genome scaffold by allowing a human-readable YAML format file to be edited instead of large sequence files. Genomer is written as a command line tool and is an effort to make the manual process of genome scaffolding more robust and reproducible. Extensive documentation and video tutorials are available at http://next.gs.     

Journal of Genetics and Genomics——Full Text Online Access

<正>Copyright■2015,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,and Genetics Society of China.Published by Elsevier Limited and Science Press.All rights reserved.Disclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal,the views and opinions expressed do not reflect those of the Sponsors,Publishers and     

Journal of Genetics and Genomics——Full Text Online Access

正Copyright■2016,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,and Genetics Society of China.Published by Elsevier Limited and Science Press.All rights reserved.Available online at www.sciencedirect.com ScienceDirectDisclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of     

Journal of Genetics and Genomics——Full Text Online Access

正Disclaimer No responsibility is assumed by the Sponsors, Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal; the views and opinions expressed do not refl ect those of the Sponsors, Publishers and Editors, neither does the publication of advertisements constitute any endorsement of the products advertised by the Sponsors, Publishers and Editors.     

Journal of Genetics and Genomics——Full Text Online Access

<正>Available online at www.sciencedirect.com ScienceDirectDisclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal;the views and opinions expressed do not reflect those of the Sponsors,Publishers and     

Journal of Genetics and Genomics——Full Text Online Access

正Disclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal:the views and opinions expressed do not reflect those of the Sponsors,Publishers and Editors,neither does the publication of advertisements constitute any endorsement of the products advertised by the Sponsors,Publishers and Editors.     

Journal of Genetics and Genomics——Full Text Online Access

<正>Available online at www.sciencedirect.com ScienceDirectDisclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal;the views and opinions expressed do not reflect those of the Sponsors,Publishers and     

Journal of Genetics and Genomics——Full Text Online Access

<正>Available online at www.sciencedirect.com ScienceDirectDisclaimer No responsibility is assumed by the Sponsors,Publishers and Editors for errors or any consequences arising from the use of information contained in the Journal;the views and opinions expressed do not reflect those of the Sponsors,Publishers and Editors,neither does the publication of advertisements constitute any endorsement of the products advertised by the Sponsors,