共查询到20条相似文献,搜索用时 15 毫秒
1.
Michael J. Thomson Keyan Zhao Mark Wright Kenneth L. McNally Jessica Rey Chih-Wei Tung Andy Reynolds Brian Scheffler Georgia Eizenga Anna McClung Hyunjung Kim Abdelbagi M. Ismail Marjorie de Ocampo Chromewell Mojica Ma. Ymber Reveche Christine J. Dilla-Ermita Ramil Mauleon Hei Leung Carlos Bustamante Susan R. McCouch 《Molecular breeding : new strategies in plant improvement》2012,29(4):875-886
Multiplexed single nucleotide polymorphism (SNP) markers have the potential to increase the speed and cost-effectiveness of genotyping, provided that an optimal SNP density is used for each application. To test the efficiency of multiplexed SNP genotyping for diversity, mapping and breeding applications in rice (Oryza sativa L.), we designed seven GoldenGate VeraCode oligo pool assay (OPA) sets for the Illumina BeadXpress Reader. Validated markers from existing 1536 Illumina SNPs and 44?K Affymetrix SNP chips developed at Cornell University were used to select subsets of informative SNPs for different germplasm groups with even distribution across the genome. A 96-plex OPA was developed for quality control purposes and for assigning a sample into one of the five O. sativa population subgroups. Six 384-plex OPAs were designed for genetic diversity analysis, DNA fingerprinting, and to have evenly-spaced polymorphic markers for quantitative trait locus (QTL) mapping and background selection for crosses between different germplasm pools in rice: Indica/Indica, Indica/Japonica, Japonica/Japonica, Indica/O. rufipogon, and Japonica/O. rufipogon. After testing on a diverse set of rice varieties, two of the SNP sets were re-designed by replacing poor-performing SNPs. Pilot studies were successfully performed for diversity analysis, QTL mapping, marker-assisted backcrossing, and developing specialized genetic stocks, demonstrating that 384-plex SNP genotyping on the BeadXpress platform is a robust and efficient method for marker genotyping in rice. 相似文献
2.
Population structure and genetic diversity in a commercial maize breeding program assessed with SSR and SNP markers 总被引:8,自引:0,他引:8
Delphine Van Inghelandt Albrecht E. Melchinger Claude Lebreton Benjamin Stich 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(7):1289-1299
Information about the genetic diversity and population structure in elite breeding material is of fundamental importance for
the improvement of crops. The objectives of our study were to (a) examine the population structure and the genetic diversity
in elite maize germplasm based on simple sequence repeat (SSR) markers, (b) compare these results with those obtained from
single nucleotide polymorphism (SNP) markers, and (c) compare the coancestry coefficient calculated from pedigree records
with genetic distance estimates calculated from SSR and SNP markers. Our study was based on 1,537 elite maize inbred lines
genotyped with 359 SSR and 8,244 SNP markers. The average number of alleles per locus, of group specific alleles, and the
gene diversity (D) were higher for SSRs than for SNPs. Modified Roger’s distance (MRD) estimates and membership probabilities of the STRUCTURE
matrices were higher for SSR than for SNP markers but the germplasm organization in four heterotic pools was consistent with
STRUCTURE results based on SSRs and SNPs. MRD estimates calculated for the two marker systems were highly correlated (0.87).
Our results suggested that the same conclusions regarding the structure and the diversity of heterotic pools could be drawn
from both markers types. Furthermore, although our results suggested that the ratio of the number of SSRs and SNPs required
to obtain MRD or D estimates with similar precision is not constant across the various precision levels, we propose that between 7 and 11 times
more SNPs than SSRs should be used for analyzing population structure and genetic diversity. 相似文献
3.
High-throughput genotyping with the GoldenGate assay in the complex genome of soybean 总被引:1,自引:0,他引:1
Hyten DL Song Q Choi IY Yoon MS Specht JE Matukumalli LK Nelson RL Shoemaker RC Young ND Cregan PB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(7):945-952
Large numbers of single nucleotide polymorphism (SNP) markers are now available for a number of crop species. However, the
high-throughput methods for multiplexing SNP assays are untested in complex genomes, such as soybean, that have a high proportion
of paralogous genes. The Illumina GoldenGate assay is capable of multiplexing from 96 to 1,536 SNPs in a single reaction over
a 3-day period. We tested the GoldenGate assay in soybean to determine the success rate of converting verified SNPs into working
assays. A custom 384-SNP GoldenGate assay was designed using SNPs that had been discovered through the resequencing of five
diverse accessions that are the parents of three recombinant inbred line (RIL) mapping populations. The 384 SNPs that were
selected for this custom assay were predicted to segregate in one or more of the RIL mapping populations. Allelic data were
successfully generated for 89% of the SNP loci (342 of the 384) when it was used in the three RIL mapping populations, indicating
that the complex nature of the soybean genome had little impact on conversion of the discovered SNPs into usable assays. In
addition, 80% of the 342 mapped SNPs had a minor allele frequency >10% when this assay was used on a diverse sample of Asian
landrace germplasm accessions. The high success rate of the GoldenGate assay makes this a useful technique for quickly creating
high density genetic maps in species where SNP markers are rapidly becoming available.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA
and does not imply approval of a product to the exclusion of others that may be suitable. 相似文献
4.
Landraces are valuable genetic resources for broadening the genetic base of elite germplasm in maize. Extensive exploitation of landraces has been hampered by their genetic heterogeneity and heavy genetic load. These limitations may be overcome by the in-vivo doubled haploid (DH) technique. A set of 132 DH lines derived from three European landraces and 106 elite flint (EF) lines were genotyped for 56,110 single nucleotide polymorphism (SNP) markers and evaluated in field trials at five locations in Germany in 2010 for several agronomic traits. In addition, the landraces were compared with synthetic populations produced by intermating DH lines derived from the respective landrace. Our objectives were to (1) evaluate the phenotypic and molecular diversity captured within DH lines derived from European landraces, (2) assess the breeding potential (usefulness) of DH lines derived from landraces to broaden the genetic base of the EF germplasm, and (3) compare the performance of each landrace with the synthetic population produced from the respective DH lines. Large genotypic variances among DH lines derived from landraces allowed the identification of DH lines with grain yields comparable to those of EF lines. Selected DH lines may thus be introgressed into elite germplasm without impairing its yield level. Large genetic distances of the DH lines to the EF lines demonstrated the potential of DH lines derived from landraces to broaden the genetic base of the EF germplasm. The comparison of landraces with their respective synthetic population showed no yield improvement and no reduction of phenotypic diversity. Owing to the low population structure and rapid decrease of linkage disequilibrium within populations of DH lines derived from landraces, these would be an ideal tool for association mapping. Altogether, the DH technology opens new opportunities for characterizing and utilizing the genetic diversity present in gene bank accessions of maize. 相似文献
5.
Elisa Bellucci Elena Bitocchi Domenico Rau Laura Nanni Nicoletta Ferradini Alessandro Giardini Monica Rodriguez Giovanna Attene Roberto Papa 《PloS one》2013,8(12)
Landraces are heterogeneous plant varieties that are reproduced by farmers as populations that are subject to both artificial and natural selection. Landraces are distinguished by farmers due to their specific traits, and different farmers often grow different populations of the same landrace. We used simple sequence repeats (SSRs) to analyse 12 barley landrace populations from Sardinia from two collections spanning 10 years. We analysed the population structure, and compared the population diversity of the landraces that were collected at field level (population). We used a representative pool of barley varieties for diversity comparisons and to analyse the effects of gene flow from modern varieties. We found that the Sardinian landraces are a distinct gene pool from those of both two-row and six-row barley varieties. There is also a low, but significant, mean level and population-dependent level of introgression from the modern varieties into the Sardinian landraces. Moreover, we show that the Sardinian landraces have the same level of gene diversity as the representative sample of modern commercial varieties grown in Italy in the last decades, even within population level. Thus, these populations represent crucial sources of germplasm that will be useful for crop improvement and for population genomics studies and association mapping, to identify genes, loci and genome regions responsible for adaptive variations. Our data also suggest that landraces are a source of valuable germplasm for sustainable agriculture in the context of future climate change, and that in-situ conservation strategies based on farmer use can preserve the genetic identity of landraces while allowing adaptation to local environments. 相似文献
6.
Single nucleotide polymorphism (SNP) genotyping is useful for assessing genetic variation in germplasm collections, genetic map development and detection of alien chromosome substitutions. In this study, a diversity analysis using 1,301 SNPs on a set of 37 barley accessions was conducted. This analysis showed a high polymorphism rate between the malting barley cultivar 'Haruna Nijo' and the food barley cultivar 'Akashinriki'. Haruna Nijo and Akashinriki are donors of the barley expressed sequence tag (EST) collections. A doubled haploid (DH) population derived from the cross between Haruna Nijo and Akashinriki was genotyped with 1,448 SNPs. Of these 1,448 SNPs, 734 were polymorphic and distributed on barley linkage groups (chromosomes) as follows: 1H (86), 2H (125), 3H (120), 4H (100), 5H (127), 6H (88) and 7H (88). By using cMAP, we integrated the SNP markers across high-density maps. The SNPs were also used to genotype 98 BC(3)F(4) recombinant chromosome substitution lines (RCSLs) developed from the same cross (Haruna Nijo/Akashinriki). These data were used to create graphical genotypes for each line and thus estimate the location, extent and total number of introgressions from Akashinriki in the Haruna Nijo background. The 35 selected RCSLs sample most of the Akashinriki food barley genome, with only a few missing segments. These resources bring new alleles into the malting barley gene pool from food barley. 相似文献
7.
Naheif E. Mohammed Carlos P. Cantalapiedra Santiago Beguería Ana M. Casas Ernesto Igartua 《Molecular ecology》2019,28(8):1994-2012
Landraces are local populations of crop plants adapted to a particular environment. Extant landraces are surviving genetic archives, keeping signatures of the selection processes experienced by them until settling in their current niches. This study intends to establish relationships between genetic diversity of barley (Hordeum vulgare L.) landraces collected in Spain and the climate of their collection sites. A high‐resolution climatic data set (5 × 5 km spatial, 1‐day temporal grid) was computed from over 2,000 temperature and 7,000 precipitation stations across peninsular Spain. This data set, spanning the period 1981–2010, was used to derive agroclimatic variables meaningful for cereal production at the collection sites of 135 barley landraces. Variables summarize temperature, precipitation, evapotranspiration, potential vernalization and frost probability at different times of the year and time scales (season and month). SNP genotyping of the landraces was carried out combining Illumina Infinium assays and genotyping‐by‐sequencing, yielding 9,920 biallelic markers (7,479 with position on the barley reference genome). The association of these SNPs with agroclimatic variables was analysed at two levels of genetic diversity, with and without taking into account population structure. The whole data sets and analysis pipelines are documented and available at https://eead-csic-compbio.github.io/barley-agroclimatic-association . We found differential adaptation of the germplasm groups identified to be dominated by reactions to cold temperature and late‐season frost occurrence, as well as to water availability. Several significant associations pointing at specific adaptations to agroclimatic features related to temperature and water availability were observed, and candidate genes underlying some of the main regions are proposed. 相似文献
8.
C. Grenier P. J. Bramel-Cox M. Noirot K. E. Prasada Rao P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):190-196
A large collection, such as the sorghum [Sorghum bicolor (L.) Moench] landrace collection held at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT),
represents a challenge for the maintenance of both the accessions of and the information documented for the germplasm collection.
The accessibility and knowledge of the landrace collection are the essential factors for an efficient utilization of the genetic
resources by both breeders and farmers. Different sampling strategies, either random or non-random, were proposed to obtain
subsets of reduced size (core collection). Three subsets were established; a random sampling within a stratified collection
(logarithmic strategy: L); a sample based upon morpho-agronomic diversity (principal component score strategy: PCS); and a
sample based upon an empirical knowledge of sorghum (taxonomic strategy: T). Comparisons of these three samples for morpho-agronomic
characterization and passport information were assessed to determine their impact on phenotypic diversity. For their overall
diversity, the three subsets did not differ, as shown with the two-dimensional representation of the morpho-agronomic diversity
and the Shannon-Weaver diversity indices. When comparisons for morpho-agronomic and passport data were considered, the PCS
subset looked similar to the entire landrace collection. The L subset showed differences for characters associated with the
photoperiod reaction that was considered in the stratification of the collection. The T subset was the most distinct from
the entire landrace collection as it over-represented the landraces selected by farmers for specific uses and covered the
widest range of geographical adaptation and morpho-agronomic characteristics.
Received: 5 October 1999 / Accepted: 3 November 1999 相似文献
9.
María Mu?oz-Amatriaín Alfonso Cuesta-Marcos Jeffrey B. Endelman Jordi Comadran John M. Bonman Harold E. Bockelman Shiaoman Chao Joanne Russell Robbie Waugh Patrick M. Hayes Gary J. Muehlbauer 《PloS one》2014,9(4)
New sources of genetic diversity must be incorporated into plant breeding programs if they are to continue increasing grain yield and quality, and tolerance to abiotic and biotic stresses. Germplasm collections provide a source of genetic and phenotypic diversity, but characterization of these resources is required to increase their utility for breeding programs. We used a barley SNP iSelect platform with 7,842 SNPs to genotype 2,417 barley accessions sampled from the USDA National Small Grains Collection of 33,176 accessions. Most of the accessions in this core collection are categorized as landraces or cultivars/breeding lines and were obtained from more than 100 countries. Both STRUCTURE and principal component analysis identified five major subpopulations within the core collection, mainly differentiated by geographical origin and spike row number (an inflorescence architecture trait). Different patterns of linkage disequilibrium (LD) were found across the barley genome and many regions of high LD contained traits involved in domestication and breeding selection. The genotype data were used to define ‘mini-core’ sets of accessions capturing the majority of the allelic diversity present in the core collection. These ‘mini-core’ sets can be used for evaluating traits that are difficult or expensive to score. Genome-wide association studies (GWAS) of ‘hull cover’, ‘spike row number’, and ‘heading date’ demonstrate the utility of the core collection for locating genetic factors determining important phenotypes. The GWAS results were referenced to a new barley consensus map containing 5,665 SNPs. Our results demonstrate that GWAS and high-density SNP genotyping are effective tools for plant breeders interested in accessing genetic diversity in large germplasm collections. 相似文献
10.
Genetic diversity in 403 morphologically distinct landraces of barley (Hordeum vulgare L. subsp. vulgare) originating from seven geographical zones of Asia was studied using simple sequence repeat (SSR) markers from regions of medium to high recombination in the barley genome. The seven polymorphic SSR markers representing each of the chromosomes chosen for the study revealed a high level of allelic diversity among the landraces. Genetic richness was highest in those from India, followed by Pakistan while it was lowest for Uzbekistan and Turkmenistan. Out of the 50 alleles detected, 15 were unique to a geographic region. Genetic diversity was highest for landraces from Pakistan (0.70 ± 0.06) and lowest for those from Uzbekistan (0.18 ± 0.17). Likewise, polymorphic information content (PIC) was highest for Pakistan (0.67 ± 0.06) and lowest for Uzbekistan (0.15 ± 0.17). Diversity among groups was 40% compared to 60% within groups. Principal component analysis clustered the barley landraces into three groups to predict their domestication patterns. In total 51.58% of the variation was explained by the first two principal components of the barley germplasm. Pakistan landraces were clustered separately from those of India, Iran, Nepal and Iraq, whereas those from Turkmenistan and Uzbekistan were clustered together into a separate group. 相似文献
11.
Padma Nimmakayala Venkata Lakshmi Abburi Abhishek Bhandary Lavanya Abburi Venkata Gopinath Vajja Rishi Reddy Sridhar Malkaram Pegadaraju Venkatramana Asela Wijeratne Yan R. Tomason Amnon Levi Todd C. Wehner Umesh K. Reddy 《Molecular breeding : new strategies in plant improvement》2014,34(2):537-548
Watermelon (Citrullus lanatus var. lanatus) is one of the most important vegetable crops in the world. Molecular markers have become the tools of choice for resolving watermelon taxonomic relationships and evolution. Increased numbers of single nucleotide polymorphism (SNP) markers together with simple sequence repeat (SSR) markers would be useful for phylogenetic analyses of germplasm accessions and for linkage mapping for marker-assisted breeding with quantitative trait loci and single genes. We aimed to construct a genetic map based on SNPs (generated by Illumina Veracode multiplex assays for genotyping) and SSR markers and evaluate relationships inferred from SNP genotypes between 130 watermelon accessions collected throughout the world. We incorporated 282 markers (232 SNPs and 50 SSRs) into the linkage map. The genetic map consisted of 11 linkage groups spanning 924.72 cM with an average distance of 3.28 cM between markers. Because all of the SNP-containing sequences were assembled with the whole-genome sequence draft for watermelon, chromosome numbers could be readily assigned for all the linkage groups. We found that 134 SNPs were polymorphic in 130 watermelon accessions chosen for diversity studies. The current 384-plex SNP set is a powerful tool for characterizing genetic relatedness and for developing medium-resolution genetic maps. 相似文献
12.
A comparison of sequence-based polymorphism and haplotype content in transcribed and anonymous regions of the barley genome. 总被引:13,自引:0,他引:13
Joanne Russell Allan Booth John Fuller Brian Harrower Peter Hedley Gordon Machray Wayne Powell 《Génome》2004,47(2):389-398
Direct estimates of sequence diversity provides an abundant source of DNA polymorphisms based on single nucleotide polymorphisms (SNPs). The frequency and distribution of nucleotide diversity within 23 genes associated with grain germination in barley were determined in a sample of accessions representing European cultivars, landraces, and wild barley accessions from throughout the fertile crescent. The overall nucleotide diversity ranged from 0.0021 to 0.0189 with a single nucleotide change being detected every 78 bp and insertion-deletion events being observed every 680 bp. Within the cultivated (H. vulgare) genepool, a small number of haplotypes were detected, the total number of haplotypes observed in H. spontaneum was almost double that detected in H. vulgare (46 and 26, respectively). Distinct haplotypes were observed in the H. spontaneum and landrace genepools, which are highly divergent from H. vulgare. A comparison of SNP-based haplotype data with EST-derived SSRs and genomic SSRs revealed a similar trend of decreasing variability in the cultivated genepool. However, the number of unique alleles identified in the cultivated sample was much greater with genomic SSRs (18%) compared with only 2.1% for SNPs and 3.8% for EST-derived SSRs. The potential utility of SNPs and EST-derived SSRs for association mapping in barley is discussed. 相似文献
13.
Molecular‐marker‐aided evaluation of germplasm plays an important role in defining the genetic diversity of plant genotypes for genetic and population improvement studies. A collection of African cassava landraces and elite cultivars was analysed for genetic diversity using 20 amplified fragment length polymorphic (AFLP) DNA primer combinations and 50 simple sequence repeat (SSR) markers. Within‐population diversity estimates obtained with both markers were correlated, showing little variation in their fixation index. The amount of within‐population variation was higher for landraces as illustrated by both markers, allowing discrimination among accessions along their geographical origins, with some overlap indicating the pattern of germplasm movement between countries. Elite cultivars were grouped in most cases in agreement with their pedigree and showed a narrow genetic variation. Both SSR and AFLP markers showed some similarity in results for the landraces, although SSR provided better genetic differentiation estimates. Genetic differentiation (Fst) in the landrace population was 0.746 for SSR and 0.656 for AFLP. The molecular variance among cultivars in both populations accounted for up to 83% of the overall variation, while 17% was found within populations. Gene diversity (He) estimated within each population varied with an average value of 0.607 for the landraces and 0.594 for the elite lines. Analyses of SSR data using ordination techniques identified additional cluster groups not detected by AFLP and also captured maximum variation within and between both populations. Our results indicate the importance of SSR and AFLP as efficient markers for the analysis of genetic diversity and population structure in cassava. Genetic differentiation analysis of the evaluated populations provides high prospects for identifying diverse parental combinations for the development of segregating populations for genetic studies and the introgression of desirable genes from diverse sources into the existing genetic base. 相似文献
14.
Mazzucato A Papa R Bitocchi E Mosconi P Nanni L Negri V Picarella ME Siligato F Soressi GP Tiranti B Veronesi F 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(5):657-669
The study of phenotypic and genetic diversity in landrace collections is important for germplasm conservation. In addition,
the characterisation of very diversified materials with molecular markers offers a unique opportunity to define significant
marker-trait associations of biological and agronomic interest. Here, 50 tomato landraces (mainly collected in central Italy),
nine vintage and modern cultivars, and two wild outgroups were grown at two locations in central Italy and characterised for
15 morpho-physiological traits and 29 simple sequence repeat (SSR) loci. The markers were selected to include a group of loci
in regions harbouring reported quantitative trait loci (QTLs) that affect fruit size and/or shape (Q-SSRs) and a group of
markers that have not been mapped or shown to have a priori known linkage (NQ-SSRs). As revealed by univariate and multivariate
analyses of morphological data, the landraces grouped according to vegetative and reproductive traits, with emphasis on fruit
size, shape and final destination of the product. Compared to the low molecular polymorphism reported in tomato modern cultivars,
our data reveal a high level of molecular diversity in landraces. Such diversity has allowed the inference of the existence
of a genetic structure that was factored into the association analysis. As the proportion of significant associations is higher
between the Q-SSR subset of markers and the subset of traits related to fruit size and shape than for all of the other combinations,
we conclude that this approach is valid for establishing true-positive marker-trait relationships in tomato.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Identification and validation of a core set of informative genic SSR and SNP markers for assaying functional diversity in barley 总被引:2,自引:0,他引:2
R. K. Varshney T. Thiel T. Sretenovic-Rajicic M. Baum J. Valkoun P. Guo S. Grando S. Ceccarelli A. Graner 《Molecular breeding : new strategies in plant improvement》2008,22(1):1-13
A ‘core set’ of 28 simple sequence repeat (SSR) and 28 single nucleotide polymorphism (SNP) markers for barley was developed
after screening six diverse genotypes (DGs) representing six countries (Afghanistan, Pakistan, Algeria, Egypt, Jordan and
Syria) with 50 SSR and 50 SNP markers derived from expressed sequence tags (ESTs). The markers of the core set are single
locus with very high quality amplifications, high polymorphism information content (PIC) and are distributed across the barley
genome. PIC values for the selected SSR and SNP markers ranged between 0.32–0.72 (average 0.58) and 0.28–0.50 (average 0.42),
respectively. To make the SNP genotyping cost effective, CAPS (cleaved amplified polymorphic sequence) and indel assays were
developed for 23 markers and the remaining 5 SNP markers were optimized for pyrosequencing. A high coefficient of correlations
(r = 0.96, P < 0.005) between the genetic similarity matrices of SSR and SNP genotyping data of the core set on diverse genotypes (DGs)
and their similar groupings according to the geographical distribution in both SSR and SNP phenograms with high bootstrap
values underline the utility and reliability of the core set. A comparative allelic and sequence diversity for SSR and SNP
markers between the DGs and six elite parental genotypes (PGs) of mapping populations showed comparable diverse nature of
two germplasm sets. However, unique SNPs and indels were observed in both germplasm sets providing more datapoints for analysing
haplotypes in a better way for the corresponding SNP marker.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
16.
To deploy a high-throughput genotyping platform in germplasm management, we designed and tested a custom OPA (Oligo Pool All), LSGermOPA, for assessing the genetic diversity and population structure of the USDA cultivated lettuce (Lactuca sativa L.) germplasm collection using Illumina’s GoldenGate assay. This OPA contains 384 EST (expressed sequence tag)-derived SNP (single nucleotide polymorphism) markers selected from a large set of SNP markers experimentally validated and mapped by the Compositae Genome Project. Used for genotyping were DNA samples prepared from bulked leaves of five randomly-selected seedlings from each of 380 lettuce accessions. High-quality genotype data were obtained from 354 of the 384 SNPs. The reproducibility of automatic genotype calls was 99.8% as calculated from the four pairs of duplicated DNA samples in the assay. An unexpectedly high percentage of heterozygous genotypes at the polymorphic loci for most accessions indicated a high level of heterogeneity within accessions. Only 148 homogenous accessions, collectively comprising all five horticultural types, were used in subsequent analyses to demonstrate the usefulness of LSGermOPA. The results of phylogenetic relationship, population structure and genetic differentiation analyses were consistent with previous reports using other marker systems. This suggests that LSGermOPA is capable of revealing sufficient levels of polymorphism among lettuce cultivars and is appropriate for rapid assessment of genetic diversity and population structure in the lettuce germplasm collection. Challenges and strategies for effective genotyping and managing lettuce germplasm are discussed. 相似文献
17.
C. Grenier M. Deu S. Kresovich P. J. Bramel-Cox P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):197-202
The large size of the sorghum [Sorghum bi-color (L.) Moench] landrace collection maintained by ICRISAT lead to the establishment of a core collection. Thus, three subsets of around 200 accessions were established from: (1) a random sampling after stratification of the entire landrace collection (L), (2) a selective sampling based on quantitative characters (PCS), and (3) a selection based on the geographical origin of landraces and the traits under farmers’ selection (T). An assessment was done of the genetic diversity retained by each sampling strategy using the polymorphisms at 15 microsatellite loci. The landraces of each subset were genotyped with three multiplex polymerase chain reactions (PCRs) of five fluorescent primer-pairs each with semi-automated allele sizing. The average allelic richness for each subset was equivalent (16.1, 16.3 and 15.4 alleles per locus for the subsets PCS, L, and T, respectively). The average genetic diversity was also comparable for the three subsets (0.81, 0.77 and 0.80 for the subsets PCS, L, and T, respectively). Allelic frequency distribution for each subset was compared with a chi-square test but few significant differences were observed. A high percentage of rare alleles (71 to 76% of 206 total rare alleles) was maintained in the three subsets. The global molecular diversity retained in each subset was not affected by a sampling procedure based upon phenotypic characters. 相似文献
18.
Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites 总被引:28,自引:0,他引:28
Two hundred and sixty maize inbred lines, representative of the genetic diversity among essentially all public lines of importance to temperate breeding and many important tropical and subtropical lines, were assayed for polymorphism at 94 microsatellite loci. The 2039 alleles identified served as raw data for estimating genetic structure and diversity. A model-based clustering analysis placed the inbred lines in five clusters that correspond to major breeding groups plus a set of lines showing evidence of mixed origins. A "phylogenetic" tree was constructed to further assess the genetic structure of maize inbreds, showing good agreement with the pedigree information and the cluster analysis. Tropical and subtropical inbreds possess a greater number of alleles and greater gene diversity than their temperate counterparts. The temperate Stiff Stalk lines are on average the most divergent from all other inbred groups. Comparison of diversity in equivalent samples of inbreds and open-pollinated landraces revealed that maize inbreds capture <80% of the alleles in the landraces, suggesting that landraces can provide additional genetic diversity for maize breeding. The contributions of four different segments of the landrace gene pool to each inbred group's gene pool were estimated using a novel likelihood-based model. The estimates are largely consistent with known histories of the inbreds and indicate that tropical highland germplasm is poorly represented in maize inbreds. Core sets of inbreds that capture maximal allelic richness were defined. These or similar core sets can be used for a variety of genetic applications in maize. 相似文献
19.
Manfred Mayer Sandra Unterseer Eva Bauer Natalia de Leon Bernardo Ordas Chris-Carolin Schön 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(11):2283-2295
Key message
Capitalizing upon the genomic characteristics of long-term random mating populations, sampling from pre-selected landraces is a promising approach for broadening the genetic base of elite germplasm for quantitative traits.Abstract
Genome-enabled strategies for harnessing untapped allelic variation of landraces are currently evolving. The success of such approaches depends on the choice of source material. Thus, the analysis of different strategies for sampling allelic variation from landraces and their impact on population diversity and linkage disequilibrium (LD) is required to ensure the efficient utilization of diversity. We investigated the impact of different sampling strategies on diversity parameters and LD based on high-density genotypic data of 35 European maize landraces each represented by more than 20 individuals. On average, five landraces already captured ~95% of the molecular diversity of the entire dataset. Within landraces, absence of pronounced population structure, consistency of linkage phases and moderate to low LD levels were found. When combining data of up to 10 landraces, LD decay distances decreased to a few kilobases. Genotyping 24 individuals per landrace with 5k SNPs was sufficient for obtaining representative estimates of diversity and LD levels to allow an informed pre-selection of landraces. Integrating results from European with Central and South American landraces revealed that European landraces represent a unique and diverse spectrum of allelic variation. Sampling strategies for harnessing allelic variation from landraces depend on the study objectives. If the focus lies on the improvement of elite germplasm for quantitative traits, we recommend sampling from pre-selected landraces, as it yields a wide range of diversity, allows optimal marker imputation, control for population structure and avoids the confounding effects of strong adaptive alleles.20.
Rodriguez M Rau D O'Sullivan D Brown AH Papa R Attene G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,125(1):171-184
Multilocus digenic linkage disequilibria (LD) and their population structure were investigated in eleven landrace populations of barley (Hordeum vulgare ssp. vulgare L.) in Sardinia, using 134 dominant simple-sequence amplified polymorphism markers. The analysis of molecular variance for these markers indicated that the populations were partially differentiated (F(ST) = 0.18), and clustered into three geographic areas. Consistent with this population pattern, STRUCTURE analysis allocated individuals from a bulk of all populations into four genetic groups, and these groups also showed geographic patterns. In agreement with other molecular studies in barley, the general level of LD was low (13% of locus pairs, with P < 0.01) in the bulk of 337 lines, and decayed steeply with map distance between markers. The partitioning of multilocus associations into various components indicated that genetic drift and founder effects played a major role in determining the overall genetic makeup of the diversity in these landrace populations, but that epistatic homogenising or diversifying selection was also present. Notably, the variance of the disequilibrium component was relatively high, which implies caution in the pooling of barley lines for association studies. Finally, we compared the analyses of multilocus structure in barley landrace populations with parallel analyses in both composite crosses of barley on the one hand and in natural populations of wild barley on the other. Neither of these serves as suitable mimics of landraces in barley, which require their own study. Overall, the results suggest that these populations can be exploited for LD mapping if population structure is controlled. 相似文献