Acriflavine-Feulgen stilbene staining: a procedure for automated cervical cytology with a television based system (LEYTAS).

A sample preparation and staining procedure for automated cytology with a TV based system (LEYTAS) is described. It consists of a centrifugation technique and automated acriflavine-Feulgen stilbene staining of cervical specimens. The advantages of using both the fluorescence and the absorption image of acriflavine-Feulgen stilbene stained cervical cells for a television based system are discussed.     

Programming obesity and poor fitness: the long-term impact of childhood television

Objective: To assess whether the long‐term effects of childhood television viewing on BMI and cardiorespiratory fitness are mediated by adult viewing. Methods and Procedures: This prospective study included an unselected birth cohort of 1,037 participants (535 men) born in Dunedin, New Zealand in 1972/1973. Hours of television viewing on weekdays were reported at ages 5, 7, 9, 11, 13, 15, and 32 years. BMI and cardiorespiratory fitness were measured at age 32 years. Results: Both childhood and adult television viewing times were significantly associated with higher BMI and lower cardiorespiratory fitness at age 32 years. Childhood television viewing was a better predictor of adult BMI and fitness than adult viewing and remained a significant predictor of these outcomes after adjusting for adult viewing time. After adjusting for adult viewing, the odds (95% confidence interval) of adult obesity increased by a factor of 1.25 (1.01, 1.53) and poor fitness increased by a factor of 1.40 (1.16, 1.70) for each hour of mean weekday television viewing during childhood. Discussion: The association between childhood television viewing and obesity and poor fitness in adulthood is not mediated by adult viewing. The detrimental health effects of watching too much television during childhood persist into adulthood. Attempts to reduce adult obesity and poor fitness by modifying television viewing habits need to begin in childhood.     

Television in contemporary urban life: Benin City,Nigeria

During 1983 and 1984 the author and her husband conducted a study of the impact of the mass media on the daily lives of the inhabitants of Benin City, Nigeria. The investigators studied television, radio, the press, and popular literature, employing a mixture of ethnographic and sociological methodologies, though a heavy stress on television was dictated by informants’ preferences for that medium, itself a finding of the study. This author was especially interested in the effects of mass communications on family life, and this paper concerns a rapid privatization of life in Benin, which has accompanied the penetration of television into the local mass market. Informants drew connections between television and significant changes in domestic life, including more private living patterns and changes in the structure of domestic authority. These changes were also reflected in a significant amount of media content. Increased urban privacy had important methodological consequences for the researchers, which are discussed in the paper.     

Telemedicine in northern Quebec.

Television transmission of diagnostic and educational information can help to improve specialized medical care in remote and underserviced areas. This paper describes a pilot study in which the Canadian satellite Anik-B was used to link the James Bay area in northern Quebec with two large Montreal teaching hospitals. Broad-band real-time television was well suited for tele-education and teleconsultation activities. A much less costly method, using narrow-band slow-scan television, was also examined, but it requires improvements. The technology of telemedicine is in place, but its future use is impeded by the prohibitive costs of operating an efficient two-way broad-band television system for several remote health care sites. A solution to this problem may be an intermediate-band system combining some of the low-cost features of narrowband slow-scan television with the interactive high-resolution advantages of broad-band real-time television.     

Television,Disordered Eating,and Young Women in Fiji: Negotiating Body Image and Identity during Rapid Social Change

Although the relationship between media exposure and risk behavior among youth is established at a population level, the specific psychological and social mechanisms mediating the adverse effects of media on youth remain poorly understood. This study reports on an investigation of the impact of the introduction of television to a rural community in Western Fiji on adolescent ethnic Fijian girls in a setting of rapid social and economic change. Narrative data were collected from 30 purposively selected ethnic Fijian secondary school girls via semi-structured, open-ended interviews. Interviews were conducted in 1998, 3 years after television was first broadcast to this region of Fiji. Narrative data were analyzed for content relating to response to television and mechanisms that mediate self and body image in Fijian adolescents. Data in this sample suggest that media imagery is used in both creative and destructive ways by adolescent Fijian girls to navigate opportunities and conflicts posed by the rapidly changing social environment. Study respondents indicated their explicit modeling of the perceived positive attributes of characters presented in television dramas, but also the beginnings of weight and body shape preoccupation, purging behavior to control weight, and body disparagement. Response to television appeared to be shaped by a desire for competitive social positioning during a period of rapid social transition. Understanding vulnerability to images and values imported with media will be critical to preventing disordered eating and, potentially, other youth risk behaviors in this population, as well as other populations at risk.     

Preparation of monolayer smears from paraffin-embedded tissue for image cytometry

A method is described for the preparation of monolayer smears from paraffin-embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin-embedded tissue this method allows retrospective studies of, for instance, the significance of DNA content with regard to the behavior of a tumor.     

Comparison of two techniques for the quantitative measurement of acid phosphatase in normal and regenerating epithelium of the female hairless hamster

Synopsis Two methods have been employed for measuring acid phosphatase activity in normal and regenerating epithelium of the female hairless hamster. The first method entails histochemical preparation followed by television scanning microdensitometric measurements using a Quantimet 720D. The second method involves incubation of freeze-dried epidermis in a suitable substrate medium followed by measurement of the fluorescence of the released product by a spectrophotofluorometer. The results were compared against each other by performing best-fit polynomial regression line analysis to the data produced by both methods. A correlation coefficient of 0.99 was obtained when interpolated data from the two best-fit curves were compared with each other. This indicates that the histochemical technique and the subsequent measurement using television scanning microdensitometry is quantitative and can, therefore, be employed in quantitative histochemistry.     

Aboriginal content: Who's got it—who needs it?

‘Aboriginal content” and “aboriginality” have become serious concepts in Australian television circles in recent years; virtually a commodity that radio and television transmitters are required to demonstrate in order to hold their licenses. Some of the peculiar issues raised by both Aboriginal television transmission, and the larger society's perception of Aboriginal culture through television, are discussed here.     

Parental Weight Status and Girls’ Television Viewing,Snacking, and Body Mass Indexes

Objective: The purpose of this study was to examine whether television viewing (TVV) provides a context for patterns of snacking fostering overweight in young girls from overweight and non‐overweight families. Research Methods and Procedures: Participants were 173 non‐Hispanic white girls and their parents from central Pennsylvania, assessed longitudinally when girls were 5, 7, and 9 years old. Path analysis was used to test patterns of relationships among girls’ TVV, snacking while watching television, snacking frequency, fat intake from energy‐dense snack food, and girls’ increase in body mass index (BMI) from age 5 to 9. Results: In both overweight and non‐overweight families, girls who watched more television consumed more snacks in front of the television. In families where neither parent was overweight, television viewing was the only significant predictor of girls’ increase in BMI. In families where one or both parents were overweight, girls who watched more television snacked more frequently, and girls who snacked more frequently had higher intakes of fat from energy‐dense snacks, which predicted their increase in BMI from age 5 to 9. TVV did not directly predict girls’ increase in BMI in girls from overweight families. Discussion: The results of this study support and extend previous findings that have shown that excessive television viewing and snacking patterns are risk factors for the development of overweight in children; however, patterns of relationships may differ based on parental weight status. For overweight families, TVV may provide a context for excessive snack consumption, in addition to inactivity.     

Forensic fictions: Science,television production,and modern storytelling

This essay uses interviews with television creators, writers, and producers to examine how media practitioners utilise, negotiate and transform forensic science in the production of televisual stories including the creation of unique visuals, character exploration, narrative progression, plot complication, thematic development, and adding a sense of authenticity. Television as a medium has its own structures and conventions, including adherence to a show’s franchise, which put constraints on how stories are told. I demonstrate how television writers find forensic science to be an ideal tool in navigating television’s narrative constraints by using forensics to create conflicts, new obstacles, potential solutions, and final solutions in their stories. I show how television writers utilise forensic science to provide the scientific certainty their characters require to catch the criminal, but also how uncertainty is introduced in a story through the interpretation of the forensics by the show’s characters. I also argue that televisual storytellers maintain a flexible notion of scientific realism based on the notion of possibility that puts them at odds with scientists who take a more demanding conception of scientific accuracy based on the concept of probability.     

A comparison of the effects of photodynamic therapy on normal and tumor blood vessels in the rat microcirculation

The effects of light activation of the tumor photosensitizer dihematoporphyrin ether (DHE) were studied in the microcirculation of the rat cremaster muscle. Arterioles and venules in an implanted chondrosarcoma were studied by in vivo television microscopy and were compared to normal vessels of the same size elsewhere in the preparation and in control preparations. Activation with green light (530-560 nm, 200 mW/cm2, 120 J/cm2) 48 h after intraperitoneal injection of DHE (10 mg/kg body wt) resulted in significant narrowing of diameters of red blood cell columns in tumor arterioles and venules. The response in normal and control arterioles and venules was not significantly different from that seen in the tumor vessels except that the control arterioles did not remain significantly constricted during the treatment period. Treatment resulted in stasis of blood flow in 90% of tumor and normal arterioles at the completion of light activation. In venules, stasis of blood flow was observed in 75% of tumor and 70% of normal vessels. Vasoconstriction was the primary response in arterioles, while thrombosis predominated in venules. Morphologic assessment of light-activated vessels in the cremaster preparation by transmission electron microscopy revealed platelet aggregation with damage to endothelial cells and smooth muscle cells. Perivascular effects observed included interstitial edema and damage to skeletal muscle cells. In the tumor-bearing preparation, no direct cytotoxic effect on the tumor cells was shown. The surrounding vessels exhibited similar vascular stasis, but the lining cells appeared minimally affected. Photoactivation of DHE results in significant changes in the microcirculation which lead to stasis of blood flow. In this model, the response was similar for the normal microvasculature and for the microcirculation of an implanted chondrosarcoma. These effects may account, in part, for the mechanism of action of photodynamic therapy.     

Televisual experiences of poverty and abundance: Entertainment television in the Philippines

This paper draws from ethnographic research on entertainment television in the Philippines, in which poverty, suffering, abundance and joy are materialised and enacted as central themes. Examples are considered from a particularly successful Philippine television program, Eat Bulaga, in which audience members compete to win prizes of cash, bank accounts, feasts, appliances or vehicles. While the production of this television program creates a wider, mediated representation of poverty and abundance, suffering and joy, the paper focuses on the practices and experiences of the people – including production staff and audience members ‐ whose participation in the making of this television program is a materially, and at times spiritually, transformative event. In doing so, Eat Bulaga consolidates and remediates a large market of television audiences whose self‐understanding incorporates the mass consumption of particular goods as being central to the Philippine experience of poverty‐in‐modernity.     

Children's Television,Radio, Internet,and Computer Usage in a City and a Village of China

A first step in understanding media consumption is to understand the time people spend using media, and how usage varies across demographic groups and in response to other factors. While there is ample research from the West, research from China is less evident. Here I provide a case study of children's media usage in a rural and an urban area in China. The findings showed that a greater proportion of children in the urban sample used media such as television, Internet, and computer games, and that rural–urban residency had the most significant influence on television viewing. Further, more urban children reported their parents had concerns about media usage, whereas a greater proportion of children in the rural area had televisions in their bedrooms and ate meals while watching television. This difference was explained by differences in socio-economic levels, traditional values, and educational background. The findings show that the rural–urban difference, and other factors such as parental concern, should be considered when conducting and interpreting media consumption. There also are implications for health because a large proportion of children in the present study had televisions in their bedrooms and ate while watching television, and such behaviors in the West have been associated with unhealthy lifestyles.     

The impact of cultural,economic, and safety issues in Flemish television news coverage (2003–13) of North African immigrants on perceptions of intergroup threat

The current research examines television news as a contextual determinant of intergroup threat from North African immigrants in Flanders (i.e. the northern part of Belgium). We content analyse the prevalence of intergroup threats in television news over an eleven-year period (2003–13), and take an experimental approach to examine the impact of these threatening news stories on perceived threats from North African immigrants. The content analysis revealed that (combinations of) safety and cultural issues were widespread in television news content. Experimental evidence showed that exposure to safety and cultural issues in news content increased perceptions of safety and cultural threat accordingly, but exposure to economic issues revealed no effect for economic threat. We conclude by emphasizing the potential of television news content in affecting perceptions of intergroup threat, particularly for those threats which are commonly present in the news and readily accessible in natives’ minds.     

A video image correlation technique for the measurement of electrophoretic mobility

The electrophoretic movement of blood cells has been examined with a video image correlator using the signal from a 625 line monochrome television camera attached to the optical arrangement. The diffraction pattern of a cell produces a characteristic signal which can be detected and registered in any television scan line to form the data of the first image frame. Under the influence of an electric field, cells moved across the television field of view and after a short interval appeared on different scan lines. The detection and registering process was repeated, and a cross-correlation function between the first and second image frame was computed. This function was stored in an output buffer which was continuously updated as fresh functions were integrated with the existing contents. The mean electrophoretic mobility of a suspension of particles can be calculated from the peak position of the integrated correlation function. Using a triangle deconvolution procedure, the relative contributions of subpopulations lymphocytes prepared from peripheral blood samples were examined.     

The Influence of Physical Activity,Socioeconomic Status,and Ethnicity on the Weight Status of Adolescents

Objective: This study examined the effects of physical activity, television viewing, video game play, socioeconomic status (SES), and ethnicity on body mass index (BMI). Research Methods and Procedures: The sample was 2389 adolescents, 10 to 16 years of age (12.7 ± 1.0 years); 1240 (52%) females and 1149 (48%) males; 77% white and 23% African American; from rural (77%) and urban (23%) settings. BMI and skinfolds were directly assessed. All other data were obtained from questionnaires. Results: Watching television on non‐school days was related to being overweight (p < 0.005). However, when BMI analyses were adjusted for ethnicity and SES, there were no significant effects of television viewing on BMI (p > 0.061). Increased hours of video game play enhanced the risk of being overweight for both genders when analyses were adjusted for ethnicity and SES (p < 0.019). In males, participation in as little as one high‐intensity physical activity 3 to 5 days a week decreased the ethnic‐ and SES‐adjusted relative risk of being overweight (RR = 0.646; CI: 0.427 to 0.977). For females, the ethnic‐ and SES‐adjusted relative risk for being overweight was not significantly altered by physical activity. The logistic analyses further indicated the influence of low SES and African American ethnicity overshadowed any direct effect of television or videos. Discussion: Because weight status of male adolescents appears to be more related to exercise habits than to television or video game habits, increased participation in high‐intensity exercise appears to be important. For females, neither videos nor exercise habits appear to be related to risk of being overweight. However, ethnicity and SES may be important factors that can influence body weight status, while television viewing may be of some importance. Thus, programs to reduce obesity in female adolescent should focus their efforts in lower SES communities.     

Vagotonicity of Violence: Biochemical and Cardiac Responses to Violent Films and Television Programmes

In a search for a reproducible means of evoking different types of emotional stress it was found that in spite of increased adrenaline secretion slowing of the heart occurred when watching violent television programmes. Further evidence of increased vagal tone was provided by the “sinus arrhythmia” effect, a widening of the gap between the maximum and minimum heart rates during the respiratory cycle in parts of the humour, violence, and suspense sections of the television programme.Groups of people taken to see two particularly violent films showed similar evidence suggesting vagal overactivity, together with increases in plasma free fatty acids and decreases in triglycerides. As these changes occurred even with β-blockade it is suggested that they might be caused by non-sympathetically mediated changes in the levels of hormones, such as growth hormone, producing lipolysis.The ability to assess objectively an individual''s reaction to viewing violence might make it possible to judge the likely social impact of violent films and television programmes.     

A semi-automated cytogenetic analysis system

Because cytogenetic evaluations involve a number of time-consuming steps, we have employed semi-automated procedures to speed up and facilitate the analysis of chromosomal aberrations in industrial workers. The system contains mechanisms for automatic focus, slide search, metaphase spread detection and relocation, centering, and X-Y coordinate memory. The equipment automatically locates and then displays a cell on a television screen; computer measurements are performed in a 0.1 second. An electronic light pen is used to mark and edit chromosomes directly on the television monitor. Preliminary studies have shown significant time-cost reduction compared with conventional procedures, and the results of analyses on the television monitor are as reliable as those from microscopic investigations.