Comparative anatomy of brain monoaminergic neurons in New World and Old World monkeys

A comparison of the distribution of brain monoamine neurons in several New World and Old World monkeys was undertaken using the Falck-Hillarp formaldehyde histofluorescence technique. The overall organization of the monoamine neurons was very similar in all species, although subtle variations were found. Catecholamine (noradrenaline and dopamine) and indoleamine (serotonin) cell bodies corresponding to groups A1–A7, A8–A10, and B1–B9, respectively were found throughout the brainstem. A few catecholamine (dopamine) cells equivalent to groups All and A12 in the diencephalon were also observed. Noradrenaline neurons, rather than those of the dopamine and serotonin systems, tended to be less numerous in the New World monkeys. Ascending catecholamine and indoleamine fiber bundles were observed in most monkeys. It is interesting that fibers corresponding to the “ventral noradrenaline bundle” appeared to be much finer in the common marmoset and tamarin than in other species. In addition, a substantial catecholamine (noradrenaline) innervation of the diencephalon was noted in all the Old World monkeys, while a much lower overall terminal density was apparent in the New World forms.     

Body size estimators in primate skeletal material

A series of twenty-three skeletal variables are tested for their utility as estimators of body size, measured as partial skeletal weight, over 286 Old World anthropoids. Several variables proved to be consistently accurate in this for the present sample: bizygomatic breadth, bicondylar femoral width, skull length, orbital width, basioninion, femoral circumference and vertebral area. The only reasonably acceptable mandibular measurement was mandibular breadth. Other variables that have been used as size estimators in previous studies proved to be less accurate.     

Blood groups of Old World monkeys,evolutionary and taxonomic implications

For practical purposes two classes of blood groups of Old World monkeys can be distinguished: human-type and simian-type, depending on the kind of reagents used for testing. Of the human-type blood groups, only the A-B-O groups, defined by saliva inhibition and serum tests, are polymorphic in some, but not all, monkey species. The distributions of those groups show wide differences not only among monkey species but also among troops of one and the same species. The tests for other human-type antigens give with the monkey red cells either uniformly positive or uniformly negative results. Thus, the human-type blood groups seem to be of limited use as taxonomic tools in the systematics of the Old World monkeys.On the other hand, the simian-type blood groups, defined by isoor crossimmune monkey sera, display highly polymorphic patterns in most species of Old World monkeys, and the capability of the antisera to react with combining groups on the red cells of monkeys of closely related species seems to reflect the taxonomic closeness of two or more species. The fact that some of the simian-type specificities, notably those belonging to the rhesus D^rh graded blood group system, are shared by many species of Old World monkeys, indicates that they were introduced into genotypes during early stages of evolution of the Cercopithecidae.     

Phylogenetic influences on hormone levels across the primate order

Adrenal and gonadal hormone levels were evaluated in representative species from Prosimii, Ceboidea, Cercopithecoidea, and Hominoidea to determine if endocrine activity was influenced by phylogenetic factors. Most small-bodied New World primates had extremely high levels of cortisol, progesterone, and testosterone when compared with Old World primates. In contrast to the high hormone levels and diversity found in Ceboidea, Old World primates showed a more similar pattern of hormone secretion. Thus, this survey supports earlier reports indicating that the callitricids and smaller cebid monkeys have a distinctive hormone profile. Although higher hormone levels tended to be associated with lower body weight, this effect was not evident in all taxa, and there were many notable exceptions. When species differ from their predicted hormone levels based on phylogenetic heritage and body weight (e.g., titi monkeys), we must look for other biological factors that influence endocrine activity. © 1992 Wiley-Liss, Inc.     

旧大陆的蒿属植物

本文报导了亚、欧、非三洲的蒿属(Artemisia Linn.)植物的系统分类、分布及区系地理等。在系统分类与分布项中,作者介绍了各学者研究该属的观点;分亚属、组的特点,亲缘关系及经订正与考证的该属298种、63变种及补遗5种、2变种的正名、异名及其分布地区。在区系地理项中,作者结合古植物孢粉证据,论述了该属的起源中心、冰期前后该属植物在地球上的迁移路线、现代分布中心、分布区密集中心及次生的起源、分化与分布中心等,同时对亚、欧、非三洲蒿属区系分10亚区、28地区成分加以论述。     

Expression of taste signal transduction molecules in the caecum of common marmosets

The extraoral presence of taste signal transduction proteins has recently been reported in rodents and humans. Here, we report for the first time the presence of these signal transduction proteins in the caecum of a non-human primate, the common marmoset. Quantitative RT-PCR data on the gene expression of taste signal transduction molecules (gustducin and TRPM5) in common marmosets suggested high expression in the caecum, which was not observed in other non-human primates. Immunohistochemical analysis confirmed the specific presence of gustducin and taste receptors in marmoset caecal cells. These results may relate to the specific feeding behaviour of marmosets, which consume plant exudates, primarily gums.     

Homoplasy and the evolution of ontogeny in papionin primates

Recent advances in developmental biology reveal that patterns of morphological development, even during early phases, may be highly susceptible to evolutionary change. Consequently, developmental data may be uninformative with regard to distinguishing homology and homoplasy. The present analysis evaluates postnatal ontogeny in papionin primates to test hypotheses about homology and homoplasy during later periods of development. Specifically, the analysis studies the allometric bases of craniometric resemblances among four papionin genera to test the hypothesis that homoplasy in adult cranial form, particularly of baboons (Papio) and mandrills (Mandrillus), is underwritten by divergent patterns of development. Bivariate and multivariate allometric analyses demonstrate that the developmental patterns in Papio baboons diverge markedly from ontogenetic allometric trajectories in other papionin species. The resemblances between Papio and Mandrillus (assuming that patterns of development in smaller papionins are ancestral) are largely consequences of perinatal increases in relative brain size in juvenile Papio. Postnatal growth to large size and strong negative allometry of neurocranial form results in shape similarities because developmental pathways for large papionin genera intersect. Analyses show that allometric data may not be particularly informative in revealing homoplasy. However, placed into proper phylogenetic context, such data illustrate derived patterns of development that may reflect critically important life-history or ontogenetic adaptations.     

Cell surface marker evaluation of infant Macaca monkey leukocytes in peripheral whole blood using simultaneous dual-color immunophenotypic analysis

Abstract: Cross-reactivity between several commercially available mouse antihuman monoclonal antibodies (mAbs), conjugated to phycoerythrin (PE) and fluorescein isothiocyanate (FITC) fluorochromes, and peripheral blood leukocyte surface antigens, has been established in infant cynomolgus (Macaca fascicularis) monkeys using whole blood lysis, and two-color, PE and FITC flow cytometric analysis. With the exception of the CD8 marker, the bivariate dot-plot patterns for all other markers were similar in infant monkeys and in humans. For the CD8 marker, however, a CD8⁺CD2^? population of cells was observed in the majority of monkeys tested (10 out of 12). The number of CD8⁺CD2^? cells was higher (13%) in infant monkeys compared to the 3% reported for adult human blood. The mean percentage and absolute numbers for the cell surface markers identified with the human mAbs CD2 (FITC, Ortho, Paritan, NJ), CD4 (PE, B-D, Mountain View, CA), and CD8 (PE, B-D) when these were combined with a series of PE- or FITC-labelled human mAbs were similar across all combinations tested. Statistically significant differences were observed between male and female monkeys for the mean percentage levels of CD4 (females > males) and for the CD4/CD8 ratio (females > males). Such gender differences need to be taken into consideration when infant cynomolgus monkeys are used as models for chemical-induced immunotoxicity studies. Measurement of the interleukin-2 (IL-2) and transferrin proved to be useful in monitoring in vitro cellular activation in infant cynomolgus and possibly in rhesus (M. mulatta) monkeys.     

A survey of the distribution and density of the primates of Guyana

In January through February 1994, we conducted the first broad-scale survey of Guyanese primates since 1975. Our goals were (1) to follow up questions raised in the earlier survey, (2) to compare population densities, and (3) to locate potential sites for future long-term research. We used distributional survey methods along trails and rivers and interviewed local inhabitants in each region. We surveyed five general areas, two of which had been studied in 1975. The distribution reported in 1975 for five monkey species—Alouatta seniculus, Cebus olivaceus, Pithecia pithecia, Chiropotes satanas,and Saimiri sciureus—was confirmed. However, questions were raised concerning the western extent of the range of three species: Ateles paniscus, Cebus apella,and Saguinus midas.In comparing densities between 1994 and 1975, we found a significant drop in group densities over the past 20 years and a shift in relative proportions of individual primate species over time. For example, although the total number of kilometers surveyed was identical, group densities were three times higher in 1975 than in 1994. Further, group densities of Ateles, Alouatta,and Pitheciawere much lower, while those of Saguinuswere similar in both years. These findings strongly suggest that habitat destruction and continued hunting pressure are affecting the primate populations.     

Metals content in placentas from moderate cigarette consumers: correlation with newborn birth weight

Cigarette consumption during pregnancy produces deleterious effects in both, mother and fetus, some of them due to the presence of toxic elements in cigarette smoke, such as cadmium. Placenta constitutes a dual-purpose specimen for evaluating the pollutant burden exerted on the mother as well as on the fetus. The main objective of this study was to establish a correlation between placental concentration and distribution of some metal elements and birth weight of neonates delivered by mothers, who were either moderate smokers or nonsmokers. Forty nonsmoking and moderate smoking pregnant women paired per age, parity, weight, height and body mass index were selected. Smoking was assessed by self-reported cigarette consumption during pregnancy and urine cotinine concentration before delivery. Placental metal concentrations were evaluated by atomic absorption spectrometry (copper and cadmium) and neutron activation analysis (zinc and iron). Newborns from smokers had lower birth weights compared to infants from nonsmokers. Birth weights were correlated with placental cadmium concentrations in both, smokers and nonsmokers. Placental zinc and cadmium of smokers were mainly located at the maternal side and their levels were higher than those found in nonsmokers placentas. In addition, all metal nutrient/pollutant ratios were decreased in the smoker group. In this first study performed in our region, we found that moderate smoking mothers deliver neonates with decreased birth weight and highly correlated to placental cadmium concentration. Decreased metal nutrient/pollutant ratios, a condition here found in smokers, may indicate a placental dysfunction, contributing to impair birth weight.     

Baseline and stress levels of cortisol in conscious and unrestrained Japanese macaques (Macaca fuscata)

A vest and tethering system was applied to Japanese macaques (Macaca fuscata) to collect blood samples under less stressful conditions. The level of plasma cortisol was assayed to assess the amount of stress. The pattern of plasma cortisol fluctuation during 24 hours was consistent under non-stress conditions. The plasma cortisol level was increased significantly by encounter and venipuncture. The increase was especially noteworthy when the experimental macaques encountered a large adult male, reflecting the strong stress on macaques. As venipuncture is a common medical procedure, and the encounter between macaques who have never met before occurs frequently, this vest and tethering system is useful for the assessment of the amount of stress in the daily care of macaques in a laboratory.