HPLC法测定芦笋茎秆中芦丁、槲皮素及白藜芦醇含量

为了建立高效液相色谱法对芦笋茎秆中芦丁、槲皮素和白藜芦醇含量的测定方法。采用不同方法提取芦笋茎秆中的芦丁、槲皮素和白藜芦醇,得出甲醇法提取芦丁、甲醇-HCl法提取槲皮素以及乙醇法提取白藜芦醇得率最高;确定了高效液相色谱法对3种物质的最佳色谱条件为流动相:芦丁为0.2 mol/L乙酸钠-甲醇(Me-OH∶H2O=35∶65)溶液(用磷酸调p H 2.80)、槲皮素为无水甲醇-0.4%磷酸溶液(55∶45,V/V),等梯度洗脱,白藜芦醇为水和甲醇,二元线性梯度洗脱;检测波长分别为:254 nm、360 nm和306 nm;流速:0.8 m L/min、1.0 m L/min、0.8 m L/min。通过该方法测得芦丁、槲皮素及白藜芦醇的平均回收率分别为:98%、97%、98%。     

HPLC法测定小叶山葡萄叶中白藜芦醇含量

建立一种高效液相色谱法(HPLC)测定小叶山葡萄Vitis thunbergii var.taiwaniana叶中白藜芦醇含量的检测方法。Waters高效液相色谱仪:Hypersil C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-水(72:28);流速:1 mL·min^-1;检测波长306 nm。结果表明,在上述色谱条件下,白藜芦醇含量在10~200μg·mL^-1范围内线性关系良好,相关系数r为0.9999。精密度、重现性、稳定性的RSD(n=5)分别为0.77%、0.41%、0.21%;平均加标回收率为99.92%,相对标准偏差为0.39%。该方法准确、灵敏、可靠,可用于白藜芦醇的定量和定性分析。     

HPLC法测定国产葡萄酒中白藜芦醇的含量

采用HPLC法测定国产葡萄酒中白藜芦醇的含量。色谱条件为 :Shim packCLC ODS柱 (15 0mm× 6 0mm) ,流动相 :0 2mol/LH3 PO4 CH3 CN(2 0∶80pH 3 0～ 3 5 ) ,在 30 6nm测定。测得 15种国产葡萄酒中通化干红葡萄酒的白藜芦醇含量最高为 5 6 0mg/L。该方法灵敏 ,重现性好     

HPLC检测三叶青块根中白藜芦醇和白藜芦醇苷含量的研究

目的：建立三叶青地下部分白藜芦醇、白藜芦醇苷以及总黄酮的提取分离和含量测定方法。方法：白藜芦醇提取分离选取溶剂为70%乙醇，白藜芦醇苷的提取分离选取溶剂为40%乙醇。含量测定采用高效液相色谱法，色谱柱选择Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm)，流动相为甲醇-水梯度洗脱，流速为0.8 mL/min，检测波长为304 nm，柱温25℃。结果：白藜芦醇的线性关系方程为Y=113.836X+0.911 5，在0.02～2μg/mL范围内的峰面积与进样浓度线性关系良好(R²=0.997 6)。采用70%乙醇提取三叶青根茎中白藜芦醇的平均含量为0.59μg/g。白藜芦醇苷的线性关系方程为Y=52.515X-0.497 7，在0.02～2μg/mL范围内的峰面积与进样浓度线性关系良好(R²=0.994 4)。采用40%乙醇提取三叶青根茎中白藜芦醇的平均含量为1.76μg/g。结论：建立了三叶青地下部分白藜芦醇、白藜芦醇苷以及总黄酮的提取分离和含量测定方法。建立的高效液相色谱法方法简便，准确，重复性好，可应用于...     

HPLC法测定宁夏枸杞中法荜枝苷的含量

本文采用高效液相色谱法,建立宁夏枸杞中法荜枝苷的含量测定方法。色谱条件:Diamonsil C8柱(150mm×4.6 mm,5μm);流动相为A:乙腈-水-醋酸(5∶94.5∶0.5),B:乙腈-水-醋酸(72∶27.5∶0.5),梯度洗脱。流速:1 mL/min;柱温:30℃;检测波长:346 nm。结果表明,在0.1～10μg/mL范围内线性关系良好,相关系数r=0.9998(n=8)。法荜枝苷的平均回收率为99.1%,RSD为1.55%。该方法准确可靠,可用于测定宁夏枸杞中法荜枝苷的含量,为宁夏枸杞开发利用提供理论依据。     

不同处理对四川牡丹种子萌发的影响

通过探究不同基质和不同浓度的GA3处理对四川牡丹种子萌发的影响,揭示四川牡丹种子萌发的最佳处理条件,旨在为四川牡丹种群扩繁和保护提供科学依据。本研究在生根阶段测定了不同处理条件(基质:细沙、椰糠、草炭土∶蛭石∶珍珠岩=1∶1∶1;GA3浓度:0、50、100、150、200 mg/L)下四川牡丹的生根参数;在发芽阶段测定分析了不同根长区间(L1:主根长度≤60 mm;L2:主根长度 60 mm)、不同基质(细沙、细沙∶黄壤=1∶1、草炭土∶蛭石∶珍珠岩=1∶1∶1)、不同浓度(0、50、100、150 mg/L)的GA3条件下四川牡丹的发芽参数。结果表明:1)生根阶段,细沙和GA3处理能使四川牡丹提前生根,提高生根率。细沙和200 mg/LG A3组合,四川牡丹生根历期最短(21d),生根率最高(1000%)。2)发芽阶段,根长区间、GA3浓度、根长区间和基质的交互作用对四川牡丹发芽率有极显著影响;四川牡丹种子发芽的优化条件为:选用主根长度 60 mm的种子,置于细沙∶黄壤=1∶1土壤中,用50mg/L GA3处理,发芽率最高(933%),发芽速率最大(516 d~(-1))。细沙和GA3处理对四川牡丹生根有促进效应。不同根长区间、基质和GA3浓度(发芽)处理可有效打破四川牡丹上胚轴休眠,缩短初萌期,提高发芽率,促进种子发芽。     

HPLC-MS/MS法快速测定人血浆中美利曲辛及生物等效性研究

目的:建立测定人血浆中关利曲辛浓度的高效液相串联质谱法(HPLC-MS/MS),用于氟哌噻吨美利曲辛片的生物等效性研究.方法:以Agilent ZORBAX Eclipse Plus C18(4.6mm× 150mm,5μm)为色谱柱,流动相为乙腈(含1%甲酸):0.02mo·L-1甲酸铵水溶液(80∶20,V∶),流速:0.8mL·min-1;柱温:40℃,以醋酸乙酯:二氯甲烷(4∶1,V∶V)为提取剂.样品经电喷雾离子源正离子化后,通过三重四级杆串联质谱仪,采用选择反应监测(SRM)对美利曲辛(m/z 292.2→232.2)和阿米替林(m/z 278.1-91.0)进行测定.结果:美利曲辛的高(50μg·L-1)、中(20μg·L-1)、低(0.5μg·L-1)3个浓度的平均回收率分别为97.53%、104.03%和106.87%,日内(n=5)、日间(n=3)RSD均小于15%;分析方法的最低定量限为0.2μg·L-1.线性范围为:0.2～60μg·L-1,回归方程为:F=1.8691ρ+0.0555,r=0.9986(n=9),权重为1/ρ2.结论:该方法灵敏、准确、简单、快速,可用于临床血浓监测和药动学研究.     

反相高效液相色谱法测定不同品种花椒中芦丁和槲皮素的含量

目的:建立RP-HPLC测定花椒中芦丁与槲皮素含量的方法,并对不同种花椒的中芦丁与槲皮素含量进行测定与比较。方法:Zorbax Eclipse C18色谱柱(150 mm×4.6 mm,5μm),流动相∶甲醇-0.4%磷酸(50∶50);流速1 mL/min;检测波长:360 nm;柱温25℃。结果:芦丁在0.25~5.0μg,r=0.999 9峰面积与质量浓度呈良好的线性关系;平均回收率为99.1%,RSD为4.3%(n=3)。槲皮素在0.25~0.5μg,r=0.999 9峰面积与质量浓度呈良好的线性关系;平均回收率为111.2%,RSD为5.1%(n=3)。结论:该方法可用于花椒中芦丁和槲皮素的测定。测定结果表明,韩城红花椒中芦丁含量最高,茂汉红花椒次之,四川青花椒较少,云南青花椒最低。槲皮素在韩城红花椒中含量较高,在其他三种花椒中差别不大。     

反相高效液相色谱法测定石榴皮多酚类物质方法的建立及分析

建立了同时测定石榴皮多酚中绿原酸、表儿茶素、鞣花酸和槲皮素含量的反相高效液相色谱方法。采用色谱柱Hypersil ODS2(250 mm×4.6 mm,5μm),其流动相为乙腈-0.4%磷酸溶液(体积比17∶83),流速为1.0m L/min,检测波长310 nm,柱温为30℃。建立安石榴甙测量方法,色谱柱为Hypersil ODS2(250 mm×4.6 mm,5μm);流动相为甲醇-2%冰醋酸(体积比7∶93);体积流量为1.0 m L/min;检测波长为232 nm;柱温25℃。结果表明:绿原酸、表儿茶素、鞣花酸、槲皮素和安石榴甙在一定浓度范围内与峰面积呈良好的线性关系,其平均回收率分别为100.66%(RSD=2.06%)、100.05%(RSD=0.58%)、100.05%(RSD=0.51%)、99.51%(RSD=1.22%)和99.79%(RSD=0.52%)。此结果说明反相高效液相色谱可用于测定石榴皮多酚类物质。     

HPLC法测定麻仁润肠丸大黄素、大黄酚的含量

采用KromacilC18(4 .6mm× 2 5 0mm ,5 μm)色谱柱 ,以甲醇 - 0 .1%磷酸溶液 (85∶15 )为流动相 ,流速为 1mL·min ,柱温为 30℃ ,检测波长为 2 5 4nm ,以外标法测定了麻仁润肠丸中大黄素、大黄酚的含量。大黄素在 8.992× 10 -3 ～ 116 .896× 10 -3 ,大黄酚在 2 1.376× 10 -3 ～ 2 77.85 8× 10 -3 范围内呈线性关系 ,其在制剂中的平均回收率 (n =6 )分别为 10 1.5 6 % (RSD =1.3% )、96 .78% (RSD =1.3% )。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.