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1.
We tested the cross-amplification of 26 microsatellites developed for passerines and an additional three developed for Gallinula species in eight European Coots from two populations. Sixteen microsatellite markers successfully amplified, of which nine
were polymorphic with 2–6 alleles (mean 3.7 alleles) and an expected heterozygosity (H
e) ranging from 0.375 to 0.805 (mean H
e = 0.589). On average, we found 2.22 alleles/locus and a mean H
e of 0.440 in one nest, and 2.56 alleles/locus and a mean H
e of 0.494 in the other one. These nine polymorphic markers could be of potential use in studies of genetic variability, population
structure and reproductive strategy of European Coots. 相似文献
2.
Caitríona E. McInerney A. Louise Allcock Mark P. Johnson Paulo A. Prodöhl 《Conservation Genetics》2009,10(5):1417-1420
Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per
locus was 11 (range 4–29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively.
Loci did not deviate from Hardy–Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites
were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies. 相似文献
3.
Fragmentation of natural habitats of the common vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated
populations. Here we describe the isolation and characteristics of 10 autosomal and one X-linked microsatellite marker. These
new markers were tested in 24 voles from a natural population in eastern Germany. Loci were highly polymorphic with numbers
of alleles per locus ranging from three to 26 and expected heterozygosities from 0.51 to 0.97. All loci except for the X-linked
locus Mar105 followed Hardy–Weinberg expectations. Cross-species amplifications revealed that most loci were polymorphic as
well in M. agrestis, M. thomasi, and M. pennsylvanicus. 相似文献
4.
We isolated and characterized seven microsatellite loci for the perennial herb Ixeridium dentatum ssp. dentatum, an apomictic triploid distributed throughout the lowland areas of East Asia. The number of alleles ranged from two to seven
in 32 screened individuals of I. dentatum ssp. dentatum from Japan. The observed and expected heterozygosities were 0.000–0.950 and 0.000–0.891, respectively, calculated using genotypes
of 20 individuals of I. dentatum ssp. nipponicum. One locus (msid4) deviated significantly from Hardy–Weinberg equilibrium (P = 0.0001). These microsatellites were also tested for cross-amplification in 11 other taxa of Lactuceae, including five endangered
taxa. These primers should be useful genetic tools not only for Ixeridium but also for other Lactuceae taxa. 相似文献
5.
Eleven microsatellite loci have been developed from Fagus longipetiolata and the loci were characterized for 21 individuals. All eleven loci were polymorphic, with 2–8 alleles and an average of
4.8 per locus. The observed (H
O) and expected (H
E) heterozygosities were 0.053–0.714 and 0.355–0.856, respectively. There was significant deviation from Hardy–Weinberg equilibrium
at two loci. No locus pair had significant linkage disequilibrium. Cross-species amplifications of the markers were also tested
in three other congeneric species. 相似文献
6.
Chaturvedi A Mohindra V Singh RK Lal KK Punia P Bhaskar R Mandal A Narain L Lakra WS 《Molecular biology reports》2011,38(5):3513-3529
We examined population structure of Labeo dero (Hamilton, 1822) from different riverine locations in India using 10 polymorphic allozyme and eight microsatellite loci.
For analysis, 591 different tissue samples were obtained from commercial catches covering a wide geographic range. Allozyme
variability (An = 1.28–1.43, Ho = 0.029–0.071) was much lower than for microsatellites (An = 4.625–6.125, Ho = 0.538–0.633).
Existence of rare alleles was found at three allozyme (MDH-2*, GPI* and PGDH*) and at two microsatellite loci (R-3* and MFW-15*). Deviation from Hardy–Weinberg equilibrium (P < 0.05, after the critical probability levels were adjusted for sequential Bonferroni adjustment) could be detected at three
loci (EST-1*, -2* and XDH*) whereas, after correction for null alleles, two microsatellite loci (MFW-1*,-15*) deviated from HWE in the river Yamuna. Fst for all the samples combined over all allozyme loci was found to be 0.059 suggesting
that 5.9% of the total variation was due to genetic differentiation while microsatellite analysis yielded 0.019 which was
concordant to mean Rst (0.02). Hierarchical partition of genetic diversity (AMOVA) showed that greater variability (approx.
95%) was due to within population component than between geographical regions. Based on distribution of genetic differentiation
detected by both markers, at least five different genetic stocks of L. dero across its natural distribution could be identified. These results are useful for the evaluation and conservation of L. dero in natural water bodies. 相似文献
7.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
8.
Marta Vila Neus Marí-Mena Shen-Horn Yen Carlos Lopez-Vaamonde 《Conservation Genetics》2010,11(3):1151-1154
Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals
from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from
5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was
0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium
at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from
this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this
highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna. 相似文献
9.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
10.
Selvaraju Kanagarajan Saraladevi Muthusamy Emily Chin-Fun Chen Hsin-Sheng Tsay 《Conservation Genetics》2009,10(5):1487-1490
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags
database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic
locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values
ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy–Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population
genetic studies in A. cinnamomea. 相似文献
11.
Wouter F. D. van Dongen Gopi K. Munimanda Jakob Augustin Donald Blomqvist Tibor Szép Richard H. Wagner 《Journal of Ornithology》2010,151(3):761-764
We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin Riparia riparia, by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which
were also polymorphic in the sand martin. Only one of these 11 loci exhibited evidence of null alleles, and all were polymorphic
(mean H
o = 0.68, range of number of alleles per locus = 4–24), making them suitable for individual heterozygosity quantification and
paternity assessment in this species (exclusion probability of 11 unlinked loci = 0.999997). 相似文献
12.
Katherine P. Munkres Line K. Bay Dean R. Jerry Mark I. McCormick Lynne Van Herwerden 《Conservation Genetics》2007,8(4):987-990
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles
per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers
developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide
repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual
relatedness. 相似文献
13.
We report ten microsatellite loci in the Emei moustache toads, Leptobrachium boringii. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Twenty-four individuals from one
breeding site were examined and ten loci were polymorphic. The number of alleles per locus ranged from 3–9 with an average
of 6.3/locus. The expected heterozygosity and observed heterozygosity ranged from 0.3874 to 0.8432, and from 0.4583 to 0.9167,
respectively. Cross-species amplification was tested in a closely related species L. leishanensis. These markers will be useful in future studies on characterizing the mating system of the species. 相似文献
14.
Chi-Hang Chan Yudi Zhao Mei-Yan Cheung Geoffrey K. Chambers 《Conservation Genetics》2008,9(4):1067-1070
The kakerori (Pomarea dimidiata) is an endangered forest bird in the Cook Islands, South Pacific. We have developed 10 microsatellite markers using kakerori
feathers as the DNA source. Seven of these loci were found to be polymorphic in 42 individuals examined. The number of alleles
per locus in the polymorphic loci varied from 3 to 5. Observed and expected heterozygosity ranges were 0.57–0.74 and 0.50–0.74,
respectively. All loci isolated conformed to Hardy–Weinberg equilibrium expectations. We believe these loci will be useful
in studying kakerori conservation genetics, and our success in developing microsatellite markers from feather samples will
encourage the use of less invasive sample sources in microsatellite isolation studies. 相似文献
15.
Panyu Hua Tingting Guo Wenchao Liu Shuyi Zhang Stephen J. Rossiter 《Conservation Genetics》2009,10(3):597-600
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe
bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these
markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and
the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population
structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful
for studying related species. 相似文献
16.
Cristaria plicata was an important freshwater mussel for pearl culture in China. 18 polymorphic microsatellite markers were isolated and characterized
using (CA)15-enriched genomic library of C. plicata. These loci showed high levels of genetic polymorphism testing on 60 individuals sampled from Poyang Lake of Jiangxi Province,
China. The number of alleles per locus ranged from 4 to 18. The expected (H
E) and observed heterozygosities (H
O) were 0.7232–0.8961 and 0.0000–1.0000, respectively. Four microsatellite loci were significantly deviated from Hardy–Weinberg
equilibrium and no linkage disequilibrium was found. These microsatellite loci will be useful for assessment of genetic diversity
and population structure in C. plicata. 相似文献
17.
Karine Monceau Maria Gaillard Estelle Harrang Diego Santiago-Alarcon Patricia. G. Parker Frank Cezilly Rémi A. Wattier 《Conservation Genetics》2009,10(5):1577-1581
Twenty-three polymorphic microsatellite loci, six dinucleotidic loci and 17 tetranucleotidic loci, were developed for the
Zenaida dove (Zenaida aurita), a bird species endemic to the Caribbean Islands. From a set of 30 individuals captured at one single location in Barbados,
we obtained 20 loci that did not deviate from Hardy–Weinberg Equilibrium. Number of alleles per locus ranged between 2 and
11 (average 7.05) and the expected heterozygosity per locus, He ranged between 0.321 and 0.881 (average 0.712). This gives an exclusionary power for parental analysis of 0.9999 and 1.0000,
knowing the genotype of one social parent, or both, respectively. Such results indicate that these 20 loci will be useful
for both studying population genetics and mate choice patterns in Z. aurita. All 20 loci amplified in four other Zenaida species, the Galápagos dove, Z. galapagoensis, the eared dove, Z. auriculata, the mourning dove, Z. macroura, the Pacific dove, Z. meloda, with 30–96% being polymorphic. 相似文献
18.
Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci
from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the
range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from
0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy–Weinberg equilibrium. These polymorphic microsatellites
will be useful for genetic diversity analysis and population structure construction for A. sibiricum. 相似文献
19.
Woo-Jin Kim Young-Ok Kim Bo-Hye Nam Hee Jeong Kong Eun-Mi Park Jung-Ha Kang Jeong-Ho Lee Kyung-Kil Kim 《Conservation Genetics》2009,10(4):1105-1111
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite
markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from
a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci
were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null
alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree
tracing and constructing a linkage map for olive flounder. 相似文献
20.
Claudete F. Ruas Thiago J. Nakayama M. Á. Ortiz Mayra A. Kuroki Tod F. Stuessy Karin Tremetsberger Eduardo A. Ruas Melissa de Oliveira Santos Salvador Talavera Paulo M. Ruas 《Conservation Genetics》2009,10(5):1413-1416
We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles
were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic
information content (PIC) was 0.533 and the observed (H
O) and expected (H
E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant
deviation from Hardy–Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm
the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris. 相似文献