共查询到20条相似文献,搜索用时 15 毫秒
1.
J.A. Simon 《Prostaglandins & other lipid mediators》1978,15(3):383-397
A radioimmunoassay for 6-keto-prostaglandin F1α has been developed. The assay is accurate and sensitive but since the antiserum cross-reacts 5–10% with prostaglandins (PGs) of the E and F series, solvent extraction and thin layer chromatography are required fo absolute specifity. The assay has been validated by comparison with a radiochemical assay and by the use of an inhibitor of 6-keto PGF1α formation, 15-hydroperoxy arachidonic acid. 6-Keto PGF1α was found to have a low cross reaction with antisera directed against PGE2, PGF2α and thromboxane B2. 相似文献
2.
Antibodies against 15 keto PGF2α and 13,14 dihydro 15 keto PGF2α were produced in goats and rabbits using the appropriate prostaglandin protein conjugate. Tritium labeled 15-keto, and 13,14 dihydro 15-keto PGF2α were prepared from 3H-PGF2α. These antibodies and 3H-labeled compounds were used to develop radioimmunoassays for the respective F2α metabolites. The antibodies had relatively little cross-reactivity (≤0.1%) with the parent F2α molecule. Infusion of PGF2α in monkeys increased 15-keto-h2 levels 10–20 fold higher than PGF2α in peripheral plasma. The levels of this metabolite were not altered detectably during clotting, indicating relatively slow rates of PGF2α metabolism in vitro. These assays should be useful to follow release rates of exogenous prostaglandins from various formulations and delivery systems, and in vivo tissue synthesis of PGF2α, where low levels preclude measuring the parent compound. 相似文献
3.
Antibodies directed against prostaglandin F2α and its main circulating metabolite, 13,14-dihydro-15-keto-prostaglandin F2α, were insolubilized by reaction with ethylchloroformate. Suspensions of insolubilized antibodies retained immunogenic activity and were useful for the development of a simple and rapid solid phase radioimmunoassay for these two prostaglandins. Using this method, suppression of prostaglandin levels by the influence of indomethacin in vivo has been observed. Advantages of solid phase radioimmunoassay over current procedures are discussed. 相似文献
4.
Shiro Ohki Katsuhiro Imaki Fumio Hirata Toshio Hanyu Nobuhiko Nakazawa 《Prostaglandins & other lipid mediators》1974,6(2):137-148
Radioimmunoassays for measuring prostaglandin F2α (PGF2α) and 5α, 7α-dihydroxy-11-keto tetranorprosta-1,16-dioic acid, PGF2α-main urinary metabolite (PGF2α-MUM), with 125I-tyrosine methylester amide (TMA) of PGF2α and PGF2α-MUM were developed.Antibody to PGF2α was produced in rabbits immunized with conjugates of PGF2α coupled to bovine serum albumine. Antibody to PGF2α-MUM was also produced in rabbits immunized with conjugates of PGF2α-MUM coupled to bovine serum albumin.PGF2α-125I-TMA had an affinity to antiserum to PGF2α. PGF2α-MUM-125I-TMA also responded to antiserum to PGF2α-MUM. 相似文献
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Antibodies against the main urinary metabolite of PGF2α in the human, 5α,7α-dihydroxy-11-ketotetranorprosta-1,16-dioic acid, were raised in rabbits. The compound was coupled selectively in the ω position to bovine serum albumin prior to injection. The resulting antibodies did not distinguish between tetranor compounds varying only in structure at the ω carbon, and thus the assay could be used also for other metabolites of PGF2α, e.g. the main urinary metabolite in the guinea pig, 5α,7α-dihydroxy-11-ketotetranorprostanoic acid. Labeled ligands for the assays were prepared either by injection of |17,18-3H|-PGF2α into humans after several days' treatment with indomethacin, or by incubation of |17,18-3H|-15-keto-13,14-dihydro-PGF2α with mitochondria from rat liver. The sensitivity of the assay was 10 pg or 4 pg with these two preparations, respectively.The assay was employed for a number of measurements: normal daily excretion in a number of humans; excretion of urinary metabolites during treatment with prostaglandin synthetase inhibitors in human subjects, or after intravenous injection of PGF2α; excretion during human pregnancy; and prostaglandin production in the guinea pig during normal estrous cycles and pregnancies and after estrogen treatment.The results of these studies were in several cases compared to similar measurements earlier performed using mass spectrometric methods, and were found to agree well. Thus, this radioimmunoassay provides a simple and accurate method for estimating prostaglandin production, particularly suitable for long-term studies and for cases where repeated blood sampling must be avoided. 相似文献
7.
Lauren M. Cagen Zahir Qureshi Hiroko Nishimura 《Biochemical and biophysical research communications》1983,110(1):250-255
Saline washed red blood cells of the toadfish convert [1-14C] arachidonic acid to products that cochromatograph with prostaglandin E2 and prostaglandin F2α. This synthesis is inhibited by indomethacin (10 μg/ml). Conversion of arachidonic acid to prostaglandin E2 was confirmed by mass spectrometry. When saline washed toadfish red blood cells were incubated with a mixture of [1-14C]-arachidonic acid and [5,6,8,9,11,12,14,15,-3H]-arachidonic acid, comparison of the isotope ratios of the radioactive products indicated that prostaglandin F2α was produced by reduction of prostaglandin E2. The capacity of toadfish red blood cells to reduce prostaglandin E2 to prostaglandin F2α was confirmed by incubation of the cells with [1-14C] prostaglandin E2. 相似文献
8.
Robert C. Corlett M.D. Boonlaw SribyattaDaniel R. Mishell Jr. M.D. Charles BallardRobert M. Nakamura Ph.D. Ian H. Thorneycroft 《Prostaglandins & other lipid mediators》1972
The abortifacient activity of prostaglandin F2α was investigated by placing one or two 50 mg tablets of prostaglandin F2α in THAM salt into the vagina of nine women less than 4 weeks pregnant at intervals of 2 to 4 hours for a 24 hour period. Serum levels of HCG, estradiol (E2), progesterone and 17α-hydroxyprogesterone were measured by radioimmunoassay prior to starting therapy and at frequent intervals thereafter for 48 hours. All but two patients had significant side-effects, mainly diarrhea and vomiting, indicating that systemic absorption took place. Although bleeding was induced in 8 of 9 women, only 3 had complete abortions. A D&C was performed on all patients 48 hours after starting therapy. A significant fall in HCG levels was noted only in the patients who aborted. Only 3 of the 9 women had significant changes in steroid levels. A fall in progesterone and 17α-hydroxyprogesterone occurred in the 3 women who aborted and took place following the fall in HCG. Estradiol levels remained in the same range in all subjects. These findings indicate that prostaglandin F2α when administered in this vehicle and this dosage is relatively ineffective as an abortifacient. When effective, its action would appear to be due to contractions of uterine muscle and not secondarily to luteolysis. 相似文献
9.
The objectives were to test the hypothesis that exogenous prostaglandin F2α (PGF2α) temporarily restores sexual behavior of castrated boars, and to evaluate effects of PGF2α on serum hormone concentrations. At 35 d after castration, nine lean-type adult boars were randomly assigned to three treatments in a 3 × 3 latin square (with three replicates). Treatments were three doses of PGF2α doses (0, 10, and 20 mg) and three periods of treatment, with 5 d between each period. Serum testosterone (T) concentrations were non-detectable at the start of the experiment. Serum concentrations of estradiol (E2), LH, prolactin (PRL), and cortisol were unaffected (P > 0.05) by PGF2α treatment. The interval from treatment to ejaculation in boars treated with 10 mg (758 s) or 20 mg (660 s) PGF2α did not differ, but were different (P < 0.05) from control boars (>1 800 s). Ejaculation duration and false mounts differed (P < 0.05) between control boars and boars treated with 10 or 20 mg PGF2α. In conclusion, PGF2α treatment did not change serum concentrations of T, E2, LH, PRL, or cortisol, but restored sexual behavior. This restoration may have been due to an effect of PGF2α directly in specific areas of the brain, or indirectly via release of other hormones that stimulated areas in the brain that affected sexual behavior. 相似文献
10.
Two cows with hydrallantois were treated with single intramuscular injection of 30 mg (cow number 1) and 25 mg (cow number 2) prostaglandin F2α (PGF2α). At about 82 hours post-injection, most of the fluids were expelled and a dead calf was delivered by forced extraction from cow number 1. Cow number 2 delivered twin calves (1 live, 1 dead) without assistance. The cow lost 225 Kg body weight (162.7 Kg fluid and 62.2 Kg weight of fetuses). Plasma progesterone was 4.45 ng/ml before PGF2α injection and 0.79 ng/ml 24 hours post-injection. No abnormal lesions or pathogens were found at necropsy of the dead calves. Fetal membranes were retained in both cows and were removed manually four days post-calving. 相似文献
11.
M.P.Eddy Moeljono Fuller W. Bazer W.W. Thatcher 《Prostaglandins & other lipid mediators》1976,11(4):737-743
Experiments were conducted to determine if prostaglandin F2α (PGF2α) is luteolytic in swine. In Experiment 1, four bilaterally hysterectomized gilts were injected with PGF2α at 0800 (10mg) and 2000 hours (10mg) and four gilts received .9% saline at the same times on day 17 after onset of estrus. Treatments were reversed in the two groups of gilts 21 days later. All eight PGF2α treated gilts exhibited estrus an average of 88.0 ± 13.5 hours after treatment and average duration of estrus was 66.0 ± 16.4 hours. Saline treated controls did not exhibit estrus. Two additional gilts were hysterectomized bilaterally and the saphenous artery catheterized on day 7 after onset of estrus. PGF2α injected on day 17 resulted in a precipitous decline in plasma progestin concentration and onset of estrus by 110 and 90 hours in gilts 1 and 2, respectively. Another bilaterally hysterectomized gilt, with CL marked with India ink, received PGF2α on day 17. Estrus occurred 92 hours later and, on day 4, regression of marked CL to corpora albicantia and presence of newly formed CL was confirmed at laparotomy.In Experiment 2, 12 bilaterally hysterectomized gilts were treated with PGF2α at 0800 (10mg) and 2000 hours (10mg) on either day 8, 11, 14 or 17 after onset of estrus. None of the gilts treated on days 8 and 11 exhibited estrus. Two of three gilts treated on day 14 and all three gilts treated on day 17 exhibited estrus at an average of 116.0 ± 9.8 hours post-treatment. Average duration of estrus was 49.6 ± 8.8 hours. 相似文献
12.
Pentti A. Järvinen Sirkka Pennanen Pekka Ylöstalo 《Prostaglandins & other lipid mediators》1973,3(4):491-504
Legal abortion was induced by intrauterine administration of prostaglandin F2α in 115 patients during the 11th – 20th week of pregnancy. An intra-amniotic method was used in 61 of the cases, an extra-amniotic one in 54 cases. The average total dose administered was 35.1 mg (range 5 – 65 mg) in the intra-amniotic group, and 6358 μg (range 1500 – 14000 μg) in the extra-amniotic group. Abortion rate was 92 % in the intra-amniotic material and 72 % in the extra-amniotic material, and side-effects, mainly gastrointestinal irritation, were noted in 74 % of the intra-amniotic cases and 54 % of the extra-amniotic ones. If total doses of 4750 μg or more were administered in the extra-amniotic cases, abortion rate went up to 80 %, but the frequency of side-effects simultaneously increased to 64 %. No serious complications occurred. Intrauterine prostaglandin induction is well suited therapeutic abortions in the second trimester, and the intra-amniotic technique is more practicable than the extra-amniotic one. The latter is applicable in cases where the puncture of the amniotic cavity is difficult to achieve, e.g. in cases of fetus mortuus and hydatiform mole. 相似文献
13.
Charles R. Wallace Terry E. Kiser George B. Rampacek Robert R. Kraeling 《Prostaglandins & other lipid mediators》1985,30(6):925-933
Half-life (), volume of distribution (Vd)_and total body clearance (TBC) of 13, 14-dihydro-15-keto PGF2α (PGFM) were measured in order to determine optimal sampling frequency for accurate measurement of PGFM. Three yearling Holstein bulls (349.2 ± 6.7 kg) and 3 yearling Holstein steers (346.7 ± 7.0 kg) were utilized in a 3 × 3 Latin square design. Animals were given 0, 25 or 50 μg PGF2α I.V.; blood samples collected every 2 min and plasma PGFM determined. The , Vd and TBC of PGFM were 2.3 ± .2 min, 43.3 ± 3.3 liters and 13.7 ± 1.9 liters/min, respectively and were similar for 25 and 50 μg doses. To determine the relationship between endogenous PGFM and LH secretion in bulls, blood samples were collected every 2 min for 12 h in 4 yearling Angus bulls (489.1 ± 11.6 kg). All animals elicited at least one LH surge and PGFM concentrations were measured in samples coincident with the LH surge. Mean plasma PGFM concentrations were greater prior to the LH surge than during the LH surge. In addition, mean plasma PGFM concentration and frequency of PGFM peaks appeared to increase prior to the LH surge suggesting an association between PGFM and pulsatile LH secretion in the bull. 相似文献
14.
William J. LeMaire William N. SpellacyAllen B. Shevach Stanley A. Gall 《Prostaglandins & other lipid mediators》1972
In a double blind study, 12 women received oxytocin for the induction of labor at term and 20 subjects received prostaglandin F2α (PGF2α). During the infusions, plasma progesterone and estriol levels were measured, and compared with pre-infusion levels of these steroids. From the analysis of the data, it is concluded that neither the infusion of oxytocin or PGF2α per se alters the plasma levels of either progesterone or estriol in term pregnant subjects. 相似文献
15.
In mammals, uterine and placental prostaglandin F2α is involved in the regulation of reproduction-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2α (PGF2α) is rapidly metabolized to its plasma metabolite PGFM (13,14-dihydro-15-keto-PGF2α), which has also been detected in urine. Therefore, the current study aimed to develop and validate an efficient, quick, and inexpensive enzyme immunoassay (EIA) for PGFM estimation in urine of the Iberian lynx (Lynx pardinus) for pregnancy monitoring and for differentiation between pregnancy and pseudo-pregnancy. Urine samples collected from captive Iberian lynx (11 pregnant and 4 pseudo-pregnant cycles) were subjected directly to a PGFM EIA. The assay was validated for parallelism, precision, and stability of urinary PGFM. In addition, high-performance liquid chromatography (HPLC) immunograms and liquid chromatography-mass spectrometry (LCMS) were performed to identify PGFM within urine samples. Urinary PGFM levels before mating and after parturition were about 1.5 ng/mL. After Day 20 postmating, both pregnant and pseudo-pregnant females showed slight increase of hormone levels; in pseudo-pregnant females, this elevation did not exceed 7 ng/mL. A significant increase in pregnant females was observed after Day 45 postmating; urinary PGFM increased from 10 ng/mL at Day 45 toward a peak of 46.0 ± 19.3 ng/mL around parturition. First results show that PGFM is detectable in feces as well and follows similar courses as shown for urine. In conclusion, the presented and validated PGFM assay is an easy and reliable method for noninvasive pregnancy diagnosis in the Iberian lynx (and probably other felids) if applied approximately 20 d prior parturition in pure urine or fecal extracts. High PGFM levels in urine or fecal samples may allow a pregnancy diagnosis without knowledge of mating time, making the PGFM test applicable to free-ranging animals. 相似文献
16.
Levels of prostaglandin F2α (PGF2α) in the amniotic fluid were determined by radioimmunoassay. Concentrations of the prostaglandin were relatively constant between 15 and 35 weeks' gestation, but an increase was observed after 36 weeks. The rise was continued up to 44 weeks. A still greater elevation of PGF2α levels was recorded during labour, when the levels were related to the amount of cervical dilatation.Amniotic fluid PGF2α levels in toxaemia of pregnancy did not significantly differ from those found in normal pregnancy. 相似文献
17.
B. Sjöquist E. Oliw I. Lundén E. ÄnggÅrd 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1979,163(1):1-8
Analysis of prostaglandin F2α (PGF2α) in urine is a useful indicator of renal prostaglandin synthesis. A mass fragmentographic method for PGF2α analysis in human urine was developed using [3,3,4,4-2H4]PGF2α as an internal standard and carrier. PGF2α was extracted from urine (20 ml) with chloroform, purified by preparative thin-layer chromatography and converted to the methyl ester trimethylsilyl ether before analysis by gas chromatograph—mass spectrometry. The specificity of the urine analysis was demonstrated by retention time and the use of two pairs of fragments m/e 494/498 and 513/517 with the same results. The coefficient of variation for duplicate analysis averaged 12.6%, n = 17. Urine from recumbent women contained 4.9 ± 2.6 (S.D.) ng/ml or 4.1 ± 1.0 ng PGF2α per mg creatinine (n = 10) with little diurnal variation. Male urine contained 5.0 ± 2.7 (S.D.) ng/ml or 3.7 ± 2.1 ng/mg creatinine (n = 10). Similar concentrations were found in boys and in girls. These observations indicate that urinary PGF2α originates from the kidneys with little contribution from the male accessory sexual glands. This method can also be applied to analysis of PGF2α in rabbit urine. 相似文献
18.
Estrus and ovulation were induced in ten mature, mixed-breed, anestrous bitches (10 to 20 kg) using exogenous gonadotropins. Bitches were bred once, on the second day of estrus. Between 11 and 13 days following estrus, bitches were bilaterally hysterectomized and randomly divided into two treatment groups of five bitches each. Four days following surgery, Group A (treated) was given a single subcutaneous injection of PGF2α (Prostin F2 alpha®) at a dose of 1 mg/kg body weight and Group B (controls) similarly given an equal volume of .9% saline. Blood samples were collected daily by cephalic venipuncture prior to surgery and for 75 days thereafter. Plasma progesterone was monitored by a radioimmunoassay method. Although bitches were teased daily following PGF2α or saline treatments, estrual behavior was not exhibited. In both the PGF2α and saline treatment groups, plasma progesterone levels showed a transient decline by 12 hours following injection, although a more dramatic decrease was observed at this time in the prostaglandin-treated bitches. Subsequently, progesterone concentrations tended to increase in both groups at 6 days following treatment, however, not to pre-treatment levels. Within 20 to 32 days following treatment in both groups, plasma progesterone levels declined to <1 ng/ml and remained depressed at least 60 days post-injection. In this study, complete luteal regression was not induced following PGF2α treatment. Luteal function in both groups, as indicated by plasma progesterone concentrations, was shortened in the absence of the uterus. 相似文献
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William E Hoffman Marc L Leavitt Ronald F Albrecht David J Miletich 《Prostaglandins & other lipid mediators》1981,21(6):899-904
Prostacyclin (PGI2), prostaglandin E2 (PGE2) and prostaglandin F2∝ (PGF2∝) were tested here in unanesthetized male Sprague-Dawley rats for their effects on the cardiovascular system as mediated by the Central nervous system. Cannulae were chronically implanted into the third cerebral ventricle, femoral arteries and femoral veins of rats. Both PGE2 and PGF2∝ induced increased arterial blood pressure and tachycardia by an action on the central nervous system. The changes seen with PGE2 were larger than those observed with PGF2∝. Only transient depressor effects were seen with PGI2 and these changes appeared to be due to the leakage of the substance into the peripheral vascular system. 相似文献