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1.
Oxygen demand elicited by the main cellular energy consumers was examined in isolated hepatocytes of sub-Antarctic (Lepidonotothen larseni) and high-Antarctic notothenioids (Trematomus eulepidotus, Trematomus pennellii, Trematomus lepidorhinus, Trematomus bernacchii, Artedidraco orianae) and in a zoarcid (Pachycara brachycephalum) fish with respect to the role of cellular metabolism in co-defining thermal tolerance. The relative proportions of energy
allocated to protein and RNA/DNA synthesis, ion regulation and ATP synthesis were quantified between 0°C and 15°C by analysis
of inhibitor sensitive cellular respiration. In all the investigated species, protein synthesis constituted 25–37%, RNA synthesis
24–35%, Na+/K+-ATPase 40–45% and mitochondrial ATP synthesis 57–65% of total respiration. The sub-Antarctic nototheniid L. larseni displayed lower cellular protein synthesis rates but somewhat higher active ion regulation activities than its high-Antarctic
confamilials, as is typical for more eurythermal species. Assumed thermal optima were mirrored in minimized overall cellular
energy demand. In the sub-Antarctic L. larseni and P. brachycephalum, minima of oxygen consumption were located between 3°C and 6°C, indicating elevated energy turnover below and above these
temperatures. In contrast, the high-Antarctic species displayed progressively rising respiration rates during warming with
a cellular energetic minimum at 0°C. The sub-Antarctic nototheniid and the zoarcid showed signs of cold-eurythermy and appear
to live close to their lower limit of thermal tolerance, while high-Antarctic notothenioids show high degrees of energetic
efficiency at 0°C. All cellular preparations maintained energy budgets over a wide thermal range, supporting the recent concept
that thermal limits are set by oxygen and associated energy limitations at the whole organism level. 相似文献
2.
The rates of RNA, protein and DNA synthesis were estimated in synchronously germinating spores ofStreptomyces granaticolor. Rapid uptake of labelled precursors of RNA and proteins was observed after 20 s. The germination process took place through
a sequence of time + ordered events. RNA synthesis started after 3 min of germination, protein synthesis began at 4 min and
net DNA synthesis at 60–70 min of germination. A characteristic feature of germination was the biphasic pattern in the rate
of RNA and protein synthesis. Spores ofStreptomyces granaticolor were sensitive to actinomycin D, rifampicin and chloramphenicol even at the start of germination. Protein synthesis during
germination was dependent on new mRNA synthesis and was independent during the first 60–70 min on replication of the spore
genome. 相似文献
3.
Application of tree leaves (C3 plants) on maize (Zea mays L.) (C4 plant) fields is an agroforestry management technology to restore or maintain soil fertility. The rate at which the tree
leaves decompose is crucial for the nutrient supply to the crop. We studied the in situ decomposition of Sesbania sesban (L.) Merr. leaves or C3 sugar for 4 – 8 days after application to a maize field in Kenya. By using the difference of around 10‰ in natural abundance
of 13C between the endogenous soil C (mainly C4) and the applied C (C3), we could calculate the contributions of the two C sources to soil respiration. The δ13C value of the basal respiration was from –15.9 to –16.7‰. The microbial response to the additions of leaves and sugar to
this tropical soil was immediate. Application of sesbania leaves gave an initial peak in respiration rates that lasted from
one to less than 6 days, after which it levelled off and remained about 2 – 3 times higher (230–270 mg C m-2 h-1) than the control respiration rates throughout the rest of the experiment (5 – 8 days). In the sugar treatment, there was
no initial peak in respiration rate. The respiration rate was 170 mg C m-2 h-1 after 4 days. At the end of the experiments, after 4–8 days, as much as 14–17% of the added C had been respired and about
60% of the total respiration was from the added sesbania leaves or C3 sugar. This non-destructive method allows repeated measurements of the actual rate of C mineralisation and facilitates decomposition
studies with high temporal resolution in the field.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
4.
Mitochondria isolated from the roots of barley (Hordeum vulgare L.) and rice (Oryza sativa L.) seedlings were capable of oxidizing external NADH and NADPH anaerobically in the presence of nitrite. The reaction was
linked to ATP synthesis and nitric oxide (NO) was a measurable product. The rates of NADH and NADPH oxidation were in the
range of 12–16 nmol min−1 mg−1 protein for both species. The anaerobic ATP synthesis rate was 7–9 nmol min−1 mg−1 protein for barley and 15–17 nmol min−1 mg−1 protein for rice. The rates are of the same order of magnitude as glycolytic ATP production during anoxia and about 3–5%
of the aerobic mitochondrial ATP synthesis rate. NADH/NADPH oxidation and ATP synthesis were sensitive to the mitochondrial
inhibitors myxothiazol, oligomycin, diphenyleneiodonium and insensitive to rotenone and antimycin A. The uncoupler FCCP completely
eliminated ATP production. Succinate was also capable of driving ATP synthesis. We conclude that plant mitochondria, under
anaerobic conditions, have a capacity to use nitrite as an electron acceptor to oxidize cytosolic NADH/NADPH and generate
ATP. 相似文献
5.
The source of nitrogen in the spores of arbuscular mycorrhizal (AM) fungi was quantified by a 15N-labeling technique. N was applied as coated urea to the soil and in solution to plant shoots. Soil-applied fertilizer had
a significant effect on spore % 15N (P<0.01), with a 24–75% contribution to spore N. Fertilizer applied to either alfalfa shoots or bahia grass shoots had little
effect on spore % 15N, accounting for 0–14% or 1–9% of spore N, respectively. These results indicate that AM fungi obtain spore N mostly from
the soil. The small amount of spore N originating from shoot-applied N may have been obtained via root exudation.
Accepted: 6 November 2000 相似文献
6.
Yike I Miller MJ Sorenson WG Walenga R Tomashefski JF Dearborn DG 《Mycopathologia》2002,154(3):139-152
In recent years cases of often fatal pulmonary hemorrhage in infants have been associated with water damaged homes and the
toxigenic fungusStachybotrys chartarum. The fungal spores contain mycotoxins which could be injurious to the rapidly developing lung. In order to understand the
developmental pathophysiology of this disease we developed an infant rat model of stachybotrytoxicosis describing the effects
of fungal spores on survival, growth, histopathology of the lung and respiration. Conidia ofS. chartarum were instilled intratracheally (1.0–8.0 × 105/gm wt.) in 4-dold Sprague-Dawley rat pups. Two control groups received either sterile PBS or a suspension of spores extensively
extracted with ethanol to remove toxins. Lethal dose response was determined (LD50 = 2.7 × 105 spores/gm wt.). All dead pups had extensively hemorrhagic lungs. Growth of surviving animals was impaired in a dose-dependent
manner. Changes of pulmonary function parameters in rats treated with 1.1 × 105 spores/g were consistent with an increased respiratory resistance. Histology of lungs revealed fresh hemorrhage, sparse hemosiderin-laden
macrophages, and evidence of inflammation including thickened alveolar septa infiltrated by lymphocytes and mononuclear cells
and intra-alveolar macrophages. Significant increases (p = 0.001) in numbers of macrophages (2-fold), lymphocytes (5-fold)
and neutrophils (7-fold) were found in BAL fluid. Hemoglobin was elevated 2-fold (p = 0.004). Proinflammatory mediator IL-1β
increased more than 6-fold and TNF-α30-fold (p = 0.001). Extracted spores had a minimal effect on all examined parameters
in BAL fluid indicating that mycotoxins are primarily responsible for the hemorrhagic and inflammatory response.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
7.
Among other temperature-sensitive mutants ofLactobacillus acidophilus the mutant “ts 9” with temperature-sensitive initiation of DNA synthesis was isolated. In this mutant, the course of DNA synthesis under
non-permissive conditions proceeds in two phases. During the first 90–120 min, a slight increase (20–50%) of DNA content takes
place. Then during further incubation at 40°C, the capacity for initiation of further DNA synthesis increases and a second
round of DNA synthesis starts after 3–4h of incubation. The initiation of DNA synthesis is prevented by chloramphenicol and
the preceding lag is temperature-dependent. It is concluded that an accumulation of an initiation factor is required for the
onset of a new cycle of DNA synthesis and that in thets 9 mutant this accumulation is inhibited at non-permissive temperature. 相似文献
8.
Measurements of stem respiration were conducted for a period of four years (1999–2002) in 14-year old Norway spruce (Picea abies [L.] Karst) trees exposed to ambient (CA) and elevated CO2 concentration (CE; ambient plus 350 μmol mol−1). Stem respiration measurements of six trees per treatment were carried out 2–3 times per month during the growing season.
Stem respiration in CE treatment was higher (up to 16 %) than in CA treatment. Temperature response of stem respiration (Q10) for the whole experimental period ranged between 1.65–2.57 in CA treatment and 2.24–2.56 in CE treatment. The mean stem
respiration rate normalized to 10 °C (R10) in CA and CE treatments ranged between 1.67–1.95 and 2.19–2.72 μmol(CO2) m−2 s−1, respectively. Seasonal variations in stem respiration were related to temperature and tree growth. 相似文献
9.
L. Adamec 《Biologia Plantarum》2003,47(3):395-402
Two main dormancy states, innate and imposed dormancy, were characterized in turions (winter buds) of the aquatic carnivorous plant Aldrovanda vesiculosa L. (Droseraceae) kept at 3 ± 1 °C in a refrigerator over the winter. As a result of the breaking of imposed dormancy by a temperature increase (at 15 – 20 °C), some of the turions rose to the water surface within 1 – 3 d and germinated. Turion leaves contained large lacunae with a slimy reticulum and were filled by water over winter. As a result of breaking imposed dormancy, the proportion of gas volume in inner turion leaves rose from 10 – 20 % to 100 % of leaf lacunae volume. The aerobic dark respiration rate of the turions [0.74 – 1.5 μmol O2) kg−1(FM) s−1] slightly increased during innate dormancy after 1 – 2 d at 20 °C, while it was almost constant during the breaking of imposed dormancy. The anaerobic fermentation rate of the turions was only 1.5 – 7 % of the oxygen respiration rate and also was constant during the breaking of imposed dormancy. In turions, the content of glucose, fructose, and sucrose was the same for the two states of dormancy, but starch content was greatly reduced for the imposed dormancy (10 – 11 vs. 32 % DM). It may be suggested that a temperature increase causes an increase of fermentation or respiration which is responsible for the evolution of gas in turion lacunae and, thus, for turion rising. 相似文献
10.
Intracellular proteolytic activity increased during incubation of the sporogenic strain ofBacillus megaterium KM in a sporulation medium together with excretion of an extracellular metalloprotease. The exocellular protease activity
in a constant volume of the medium reached a 100-fold value with respeot to the intracellular activity. Maximal values of
the activity of both the extracellular and intracellular enzyme were reached after 3 – 5 h of incubation. After 7 h 20 – 50%
cells formed refractile spores. The intracellular proteolytic system hydrolyzed denatured proteinsin vitro at a rate up to 150 μg mg-1 h-1 and native proteins at a rate up to 70 μg mg-1 h-1. Degradation of proteinsin vivo proceeded from the beginning of transfer to the sporulation medium at a constant rate of 40 μg mg-1 h-1 and the inactivation of beta-galactosidase at a rate of 70 μg mg-1 h-1. The intracellular proteolytic activity was inhibited to 65 – 88% by EDTA, to 23 – 76% by PMSF. Proteolysis of denatured
proteins was inhibited both by EDTA and PMSF more pronouncedly than proteolysis of native proteins; 50 – 65% of the activity
were localized in protoplasts.
Another strain ofBacillus megaterium (J) characterized by a high (up to 90%) and synchronous sporulation activity was found to behave in a similar way, but the
rate of protein turnover in this strain was almost twice as high. The asporogenic strain ofBacillus megaterium KM synthesized the exocellular protease in the sporulation medium, but its protein turnover was found to decrease substantially
after 3 – 4 h. The intraeellular proteolytic system of the sporogenic strain J and the asporogenic strain KM were also inhibited
by EDTA and PMSF. 相似文献
11.
J. Vijaya Kumar B. D. Ranjitha Kumari G. Sujatha Enrique Castaño 《Plant Cell, Tissue and Organ Culture》2008,93(1):85-96
The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower
(Carthamus
tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium
with different levels of FCF (10–50%) produced embryogenic callus. In organogenesis, 42.2% microshoots formed directly from
embryogenic callus tissues in plant regeneration medium with 40% FCF. Isolated embryogenic callus cultured on embryo induction
medium containing 40% FCF induced 50.2% somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28
in embryo maturation medium containing 40% FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis
were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were
more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion
length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived
FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7–0.5 mm
when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT)
activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42% (0.19 μmol min−1 mg−1 protein) from organogenesis and 47% (0.23 μmol min−1 mg−1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17% (149 U
mg−1 protein) and 19.5% (145 U mg−1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants. 相似文献
12.
I. Morris 《Archives of microbiology》1966,54(2):169-176
Summary A chloramphenicol concentration of 3 mg per ml inhibits uptake of 14C-labelled phenylalanine, lysine, and adenine by Chlorella cells. Incorporation into both the free pool and the TCA insoluble fraction is inhibited. The inhibition is not related to inhibition of protein synthesis since cycloheximide (a specific inhibitor of protein synthesis in Chlorella) does not inhibit uptake of the 14C-labelled amino acids. Uptake of 14C-uracil is not inhibited by chloramphenicol.Both chloramphenicol and 2.4-dinitrophenol stimulate endogenous respiration of Chlorella, but whereas the latter reduces the internal concentration of ATP, the former (in concentrations of 1–3 mg/ml) stimulates it about two-fold. Similar concentrations of chloramphenicol decreases slightly the concentration of ADP, and it is therefore suggested that in Chlorella, chloramphenicol concentrations of 1–3 mg per ml inhibit some energy-linked reactions by preventing ATP utilization. 相似文献
13.
Inoculation of containerized Pseudotsuga menziesii and Pinus pinaster seedlings with spores of five species of ectomycorrhizal fungi 总被引:3,自引:3,他引:0
Container-grown Pseudotsuga menziesii and Pinus pinaster seedlings were inoculated with water suspensions of spores of five ectomycorrhizal fungi commonly found in northeastern Spain.
Pseudotsuga menziesii seedlings were inoculated with basidiospores of Melanogaster ambiguus, or Rhizopogon subareolatus, or with ascospores of Tuber maculatum. Pinus pinaster seedlings were inoculated with basidiospores of Melanogaster ambiguus, Rhizopogon roseolus or Scleroderma citrinum. The spore concentrations were 102–107 spores per seedling for Melanogaster ambiguus (in Pseu
dotsuga menziesii) and Rhizopogon subareolatus, 103–107 for Melanogaster ambiguus (in Pinus pinaster), Rhizopogon
roseolus, and Scleroderma citrinum, and 102–104 for Tuber maculatum. Melanogaster ambiguus colonized more short roots in a larger proportion of plants at 107 spores per seedling than at any other rate. The highest colonization by Rhizopogon subareolatus was obtained at 104 spores per seedling and higher, and all inoculated plants became infected at 106 spores per seedling and higher. Tuber maculatum colonized a high percentage of short roots at all rates tested; the proportion of infected plants was over 80% at 103–104 spores per plant, decreasing to 50% at 102 spores per plant. Rhizopogon roseolus colonized the highest number of short roots on nearly all the inoculated plants when applied at 105 spores per seedling and higher. Scleroderma citrinum colonized a high percentage of short roots on all inoculated plants when applied at 105 spores per seedling and higher. The abundance of sporocarps of Melanogaster ambiguus, Rhizopogon subareolatus, R
hizopogon roseolus and Scleroderma citrinum and their colonization ability at relatively low rates allows these spores to be used as ectomycorrhizal inocula on a large
scale.
Accepted: 27 February 1996 相似文献
14.
The action of antibiotics on the anaerobic digestion process 总被引:3,自引:0,他引:3
Antibiotics can disturb the production of biogas during anaerobic digestion. This study shows a systematic approach to understanding
how the different bacterial populations involved in the final conversion of organic matter into methane are inhibited by 15
antimicrobial agents with different specificities and modes of action. The results obtained show the following trends: (i)
some inhibitors, such as the macrolide erythromycin, lack any inhibitory effect on biogas production; (ii) some antibiotics,
with different specificities, have partial inhibitory effects on anaerobic digestion and decrease methane production by interfering
with the activity of propionic-acid- and butyric-acid-degrading bacteria,␣(e.g. antibiotics that interfere with cell wall
synthesis, RNA polymerase activity and protein synthesis, especially the aminoglycosides); (iii) the protein synthesis inhibitors
chlortetracycline (IC50 40 mg l−1) and chloramphenicol (IC50 15–20 mg l−1) are very powerful inhibitors of anaerobic digestion. The majority of the antibiotics tested lacked activity against acetoclastic
methanogens, being active only on the acetogenic bacteria. However, chloramphenicol and chlortetracycline could cause the
complete inhibition of the acetoclastic methanogenic archaea.
Received: 6 February 1996 / Received revision: 24 July 1996 / Accepted: 5 August 1996 相似文献
15.
Sparse Ulmus pumila woodlands play an important role in contributing to ecosystem function in semi-arid grassland of northern China. To understand
the key attributes of soil carbon cycling in U. pumila woodland, we studied dynamics of soil respiration in the canopy field (i.e., the projected crown cover area) and the open
field at locations differing in distance (i.e., at 1–1.5, 3–4, 10, and >15 m) to tree stems from July through September of
2005, and measured soil biotic factors (e.g., fine root mass, soil microbial biomass, and activity) and abiotic factors [e.g.,
soil water content (SWC) and organic carbon] in mid-August. Soil respiration was further separated into root component and
microbial component at the end of the field measurement in September. Results showed that soil respiration had a significant
exponent relationship with soil temperature at 10-cm depth. The temperature sensitivity index of soil respiration, Q
10, was lower than the global average of 2.0, and declined significantly (P < 0.05) with distance. The rate of soil respiration was generally greater in the canopy field than in the open field; monthly
mean of soil respiration was 305.5–730.8 mg CO2 m−2 h−1 in the canopy field and 299.6–443.1 mg CO2 m−2 h−1 in the open field from July through September; basal soil respiration at 10°C declined with distance, and varied from ~250 mg
CO2 m−2 h−1 near tree stems to <200 mg CO2 m−2 h−1 in the open field. Variations in soil respiration with distance were consistent with patterns of SWC, fine root mass, microbial
biomass and activities. Regression analysis indicated that soil respiration was tightly coupled with microbial respiration
and only weakly related to root respiration. Overall, variations in SWC, soil nutrients, microbial biomass, and microbial
activity are largely responsible for the spatial heterogeneity of soil respiration in this semi-arid U. pumila woodland. 相似文献
16.
Henk M. Jonkers Michael Jansen Marc J. E. C. Van der Maarel H. Van Gemerden 《Archives of microbiology》1999,172(3):150-156
This is the first report describing the complete oxidation of dimethyl sulfide (DMS) to sulfate by an anoxygenic, phototrophic
purple sulfur bacterium. Complete DMS oxidation was observed in cultures of Thiocapsa roseopersicina M11 incubated under oxic/light conditions, resulting in a yield of 30.1 mg protein mmol–1. No oxidation of DMS occurred under anoxic/light conditions. Chloroform, methyl butyl ether, and 3-amino-1,2,4-triazole,
which are specific inhibitors of aerobic DMS oxidation in thiobacilli and hyphomicrobia, did not affect DMS oxidation in strain
M11. This could be due to limited transport of the inhibitors through the cell membrane. The growth yield on sulfide as sole
electron donor was 22.2 mg protein mmol–1 under anoxic/light conditions. Since aerobic respiration of sulfide would have resulted in yields lower than 22 mg protein
mmol–1, the higher yield on DMS under oxic/light conditions suggests that the methyl groups of DMS have served as an additional
carbon source or as an electron donor in addition to the sulfide moiety. The kinetic parameters V
max and K
m for DMS oxidation under oxic/light conditions were 12.4 ± 1.3 nmol (mg protein)–1 min–1 and 2 μM, respectively. T. roseopersicina M11 also produced DMS by cleavage of dimethylsulfoniopropionate (DMSP). Specific DMSP cleavage rates increased with increasing
initial substrate concentrations, suggesting that DMSP lyase was only partly induced at lower initial DMSP concentrations.
A comparison of T. roseopersicina strains revealed that only strain M11 was able to oxidize DMS and cleave DMSP. Both strain M11 and strain 5811 accumulated
DMSP intracellularly during growth, while strain 1711 showed neither of these characteristics. Phylogenetic comparison based
on 16S rRNA gene sequence revealed a similarity of 99.0% between strain M11 and strain 5811, and 97.6% between strain M11
and strain 1711. DMS and DMSP utilization thus appear to be strain-specific.
Received: 26 March 1999 / Accepted: 18 June 1999 相似文献
17.
The effects of environmental factors on seasonal and annual variations in soil respiration were examined in the cool temperate
Zoysia japonica grassland of Japan. Field measurements of soil respiration were conducted using a closed chamber method with an infrared
gas analyzer at monthly intervals in the snow-free seasons from May 2007 to December 2009. There was an exponential relationship
between soil respiration and soil temperature, and the soil temperature accounted for 85–86% of seasonal soil respiration
variability. Moreover, a positive linear relationship between soil respiration and soil water content was detected in summer
(R
2 = 0.55, p < 0.001), but not in spring or autumn. Annual soil respiration was estimated at 755, 719, and 1,037 g C m−2 year−1 in 2007, 2008, and 2009, respectively. These interannual variations in soil respiration might be influenced by the strength
of precipitation during rainy seasons and the timing of each snow-melt. Our results suggest that the effects of rainfall and
snow-melt events on soil respiration might be important factors to understand carbon dynamics in grassland ecosystem in Japan. 相似文献
18.
Summary The endogenous respiration of 14C-labelled spores of B. cereus was measured through the 14CO2 produced, and the rate expressed as Q (l CO2/hxmg). New upper limits for respiration in various conditions have been set.Dry spores had no measurable activity; Q<10–4 at room temperature and <10–3 at 35° C. For wet spores of different harvests, at 30°C, Q lay between 0.0013 to 0.067. Near 40° C, respiration showed a maximum. Thermal history has a great influence on Q. CO2 production by heat-killed spores is attributed largely to infection.Water or 10–3
m sodium phosphate buffer (pH=6.5) gave equal spore respiration, in strong NaCl it was less. Azide enhanced respiration dramatically. A temporary increase was also found with non-radioactive glucose. Exogenous respiration of spores in glucose exceeded endogenous respiration.Endogenous and exogenous respiration of vegetative forms were much larger than those of spores and were time-dependent. The ratio of minimum (endogenous, dry spores) and maximum (exogenous, wet vegetative cells) respiration was at least 3x105. 相似文献
19.
Ravendra Pal Singh 《Journal of biosciences》1982,4(4):431-439
Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein
was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before
dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75–85%) during spore germination. No appreciable amount of the
inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an
activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses
both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis. 相似文献
20.
O. V. Ermokhina G. G. Belkina Yu. P. Oleskina S. G. Fattakhov N. P. Yurina 《Applied Biochemistry and Microbiology》2009,45(5):550-554
The effects of growth regulator of the new generation—melamine salt of bis(oxymethyl)phosphine acid (melaphene)—on culture
growth, pigment and protein content, and the induction of protective chloroplastic chaperone HSP70B in Chlamydomonas reinhardtii CW15 cells were studied. Melaphene exhibited 10–30% growth inhibition at 10−9–10−2% concentration. At 10−9–10−4% of melaphene electrophoretic concentration, the pattern of cellular proteins was similar to the control. The alterations
in protein content of algae cells were detected only at 10−2% concentration. The content of chlorophyll and carotenoids in melaphene-treated cells was 17–40% lower than in the control.
Melaphene at 10−9–10−2% concentration inhibited HSP70B induction by 39–43% compared to untreated cells. The potential mechanism of melaphene effect
might involve its influence on nuclear gene expression. 相似文献