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天然及氧化修饰脂蛋白对人动脉平滑肌细胞原癌基因表达的影响 总被引:10,自引:0,他引:10
动脉平滑肌细胞(SMC)的增殖在动脉粥样硬化(AS)的形成过程中极其重要。我们在建立人主动脉SMC体外培养方法的基础上,观察了LDL,VLDL及HDL和相应的氧化修饰型脂蛋白对培养人SMCsis,jun,H-ras原癌基因及Rb抗癌基因转录表达的影响。结果表明:(1)HDL对SMCsis,jun,ras基因表达无影响;(2)LDL和VLDL有使这些基因表达增加的趋势;(3)ox-LDL,ox-VLDL和ox-HDL具有使SMCsis,jun,和ras基因表达显著增强的作用(P<0.01),且其作用较相应的天然脂蛋白大(P<0.01);(4)天然和氧化修饰型脂蛋白对Rb基因表达均无影响。据上述结果推测:LDL,VLDL,ox-LDL,ox-VLDL和ox-HDL的致AS作用可能与刺激SMCsis,jun和ras原癌基因表达增加有关。 相似文献
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本文分析了广义山蚂蝗属6种1变种的核型,并报道了9种1亚种的染色体数目。假地豆Desmodiumheterocarpon(L.)DC.,伏毛假地豆D.heterocorpo。(L.)DC.var.strigosumvanMeeuwen,单叶假地豆D.rubrum(Lour.)DC..金钱草D.styracifolium(Osbeck.)Merr.及假木豆Dendrolobiumtriangulare(Retz.)Schindl.的核型均为K(2n)=22=22m,属1A类型.但它们的染色体相对长度变化范围有一定的差异,假木豆的较大,假地豆的较小。舞草Codoriocalyxmotorius(Houtt.)Ohashi和圆叶舞草C.gyroides(Roxb.exLink.)Hassk.的核型为K(2n)=22=22m,有的细胞可见随体染色体,属1B类型.根据核型资料比较.作者发现狭义的舞草属比狭义的山蚂螟属和假木豆属较为进化。本文还报道大叶山蚂蝗DesmodiumlaxiflorumDC,波叶山蚂蝗D.sequaxWall.绒毛山蚂蝗D.velutinum(Willd.)DC,异叶山绿豆D.heteroph? 相似文献
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山东樱属一新变种——泰山野樱花 总被引:1,自引:0,他引:1
泰山野樱花Cerasusserulata(Lindl.)G.DonexLondonvar.taishanensisY.ZhangetC.D.Shi,var.nov.TYPE:China.Shandong(山东),Taian(泰安),Mt.Taisha... 相似文献
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ChangesofConAReceptorSitesonMammalianSpermsduringCapacitationandAcrosomeReactionDUANChong-wen(段崇文),CHENDa-yuan(陈大元)(StateKeyL... 相似文献
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本文发现线粒体H^+-ATPase复合体先用0.5ug/ml的DCCD(二环已基碳二亚胺预保温处理,再经12.5%(V/V)乙醇进一步保温处理,则乙醇可完全消除DCCD引起的H^+-ATPase的抑制效应。若H^+-ATPase用DCCD和乙醇同时预保温处理,则DCCD同样消失其抑制作用。用相同浓度的甲醇代替乙醇,则仅可部分的消除DCCD的抑制作用。用相同浓度的DMSO(二甲基亚砜)代替乙醇,则不 相似文献
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部分裸子植物叶片总蛋白分析 总被引:1,自引:0,他引:1
采用SDS- PAGE 技术, 分析了红豆杉科(Taxaceae) 植物南方红豆杉( Taxus chinensisvar- mairei (Lemee et Levl-) Cheng et L-K-Fu) 、穗花杉( Amentotaxus argotaenia (Hance) Pil ger) 、云南穗花杉( A- yunnanensis Li) 、白豆杉( Pseudotaxuschienii(Cheng) Cheng) 以及三尖杉科(Cephalotaxaceae) 、植物三尖杉( Cephalotaxus fortunei Hook-f-) 、粗榧( C-sinensis (Rehd-etWils-) Li) 、海南粗榧( C-hainanensis Li) 、篦子三尖杉( C-oliveri Mast-) 和罗汉松科(Podocarpaceae) 、植 物罗汉松 ( Podocarpus macrophyllus ( Thunb- ) D-Don) 、鸡毛 松( P-imbricatus Bl-) 、竹柏( P- nagi(Thunb-) Zoll) 、陆均松( Dacrydium pierrei Hickel) 共12 种植物的叶片蛋白, 在蛋白质水平上采用 相似文献
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天然及氧化修饰脂蛋白对人动脉平滑肌细胞原癌基因… 总被引:8,自引:1,他引:8
动脉平滑肌细胞(SMC)的增殖在动脉粥样硬化(AS)的形成过程中极其重要。我们在建立人主动脉SMC体外培养方法的基础上,观察了LDL,VLDL及HDL和相应的氧化修饰型脂蛋白对培养人SMC sis,jun,H-ras原癌基因及Rb抗癌基因转录表达的影响。结果表明:(1)HDL对SMCsis,jun,ras基因表达无影响;(2)LDL和VLDL有使这些基因表达增加的趋势;(3)ox-LDL,ox-V 相似文献
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DynamicChangesofβTubulinduringtheResumptionofMeiosisofMouseOocyteLIUHui;(刘辉)CHENDa-yuan;(陈大元)(StateKeyLaoboratoryofReproducti... 相似文献
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氧化修饰HDL刺激培养人主动脉平滑肌细胞增殖 总被引:2,自引:0,他引:2
平滑肌细胞(smooth muscle cell,SMC)增殖在动脉粥样硬化(atherosclerosis,AS)形成中起着重要作用。氧化修饰HDL(oxidixed HDL,OX-HDL)可刺激^3H-TdR掺入培养人动脉SMC的DNA,促进SMC增殖。以四甲工偶氮唑盐9MTT)法直接观察OX-HDL对培养人动脉SMC增殖细胞数的影响。结果显示,天然HDL(native HDL N-HDL)对 相似文献
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K. Hausmann 《Protoplasma》1979,100(2):199-213
Summary The membranes of the pellicle of the ciliatePseudomicrothorax dubius are investigated using thin section electron microscopy and freeze-fracture replicas. The plasma membrane is covered by a surface coat and is connected to the outer alveolar membrane by short, sometimes branched, bridges. The inner alveolar membrane is coated on both sides. The epiplasm lies in intimate contact with the cytoplasmic surface of this membrane, and there is a corresponding deposit on the other surface. This deposit is regularly striated.The epiplasmic layer and the alveoli are interrupted at sites of cytotic activity,e.g., the attachment sites of trichocysts, the cytoproct, and the parasomal sacs. The striated deposit ends where the epiplasm ends, indicating a direct relationship between these two epimembranous layers.There is a deposit along the sides of the first part of the tip of the trichocysts, and in this region the trichocyst membrane is free of intramembranous particles.The membrane of the parasomal sacs has a coat on both surfaces. That on the extraplasmic surface is similar to the surface coat of the plasma membrane. The origin of the cytoplasmic coat is unknown. The cytotic activity of these sacs is indicated by their highly irregular profiles. 相似文献
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Summary The differentiation of the spermatid, especially in reference to the formation of the flagellum, and transformation of the shape of the nucleus was investigated in the domestic fowl.In the early stage of the spermatid, a prominent Golgi apparatus appears around the centrioles. The Golgi vesicles then surround the axial-filament complex which develops from the distal centriole. These vesicles fuse to form continuous membrane at the earliest stage of flagellar formation, and in the succeeding stage Golgi lamellae are attached to the plasma membrane of the developing flagellum. From these observations, it is assumed that Golgi apparatus may be a source of the membrane system of the flagellum.The microtubules distributed around the nucleus form the circular manchette. The anterior region of the nucleus with the manchette is cylindrical in shape and the posterior region without it remains irregular in shape. When the circular manchette has been completed, the whole nucleus acquires a slender cylindrical shape. The circular manchette then changes into the longitudinal manchette. The nuclei of spermatids without a longitudinal manchette are abnormal in shape. In view of these observations it is assumed that the nuclear shaping of the spermatid may be accomplished by circular manchette and the maintenance of shape of the elongated nucleus by longitudinal manchette.The authors wish to thank Mr. Takayuki Mori for his helpful suggestions and technical advices 相似文献
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Panova IG Sharova NP Dmitrieva SB Levin PP Tatikolov AS 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2008,151(4):676-681
This study aimed to analyze the aqueous humor (AH) and the vitreous body (VB) of the eye of the adult frog Rana temporaria L. as a representative species of amphibians, which lead a semi-terrestrial life. The presence of collagen, albumin, uric acid and electron donors was shown in both media; however, there are slight differences in their concentrations. To determine collagen, a spectral-fluorescent probe, cyanine dye, was used. The presence of collagen in AH of the frog was found at the first time. The total content of electron donors (ascorbic and uric acids, tryptophan, and tyrosine) in VB and HA was roughly estimated at ~ 1.5 × 10− 4 mol/L. Both VB and AH absorb light in similar UV regions. The total protein and albumin contents in AH were found to be somewhat higher than those in VB. The uric acid content was at an equally low level in both intraocular media. It is supposed that the similarity of VB and AH compositions shown in this work is due to some exchange between VB and AH contents in the course of accommodation. The role of intraocular fluids in physiological functions of the eye and in protecting the retina against UV light is discussed. 相似文献
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Summary The choriocapillaris is a fenestrated capillary bed located posterior to the retinal pigment epithelium. It serves as the main source of supply to the photoreceptors, retinal pigment epithelium, and other cells of the outer retina. The permeability of these capillaries to intravenously injected ferritin (MW — approx. 480,000; mol. diam. 11 nm) was examined in the mouse, rabbit, and guinea pig, each of which is characterized by a different type of retinal vascularization. In all three species, the bulk of the ferritin remained in the capillary lumina, where it appeared to be blocked at the level of the diaphragmed fenestrae. Some ferritin was present in endothelial cell vacuoles. The results confirm previous work on the rat choriocapillaris and indicate that the barrier function of the choriocapillary endothelium is present even among species in which the retinal circulation differs significantly.Supported by NIH grant EY03418 相似文献