共查询到20条相似文献,搜索用时 31 毫秒
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The human transcriptional repressor protein NAB1: expression and biological activity 总被引:4,自引:0,他引:4
Thiel G Kaufmann K Magin A Lietz M Bach K Cramer M 《Biochimica et biophysica acta》2000,1493(3):289-301
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In vivo mutational analysis of the NGFI-A zinc fingers. 总被引:4,自引:0,他引:4
T E Wilson M L Day T Pexton K A Padgett M Johnston J Milbrandt 《The Journal of biological chemistry》1992,267(6):3718-3724
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Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins 总被引:6,自引:0,他引:6
Palmer S Brouillet JP Kilbey A Fulton R Walker M Crossley M Bartholomew C 《The Journal of biological chemistry》2001,276(28):25834-25840
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To analyze the DNA binding domain of E coli LexA repressor and to test whether the repressor binds as a dimer to DNA, negative dominant lexA mutations affecting the binding domain have been isolated. A large number of amino acid substitutions between amino acid positions 39 and 46 were introduced using cassette mutagenesis. Mutants defective in DNA binding were identified and then examined for dominance to lexA+. A number of substitutions weakened repressor function partially, whereas other substitutions led to a repressor with no demonstrable activity and a defective dominant phenotype. Since the LexA binding site has dyad symmetry, we infer that this dominance results from interaction of monomers of wild-type LexA protein with mutant monomers and that an oligomeric form of repressor binds to operator. The binding of LexA protein to operator DNA was investigated further using a mutant protein, LexA408, which recognizes a symmetrically altered operator mutant but not wild-type operator. A mixture of mutant LexA408 and LexA+ proteins, but neither individual protein, bound to a hybrid recA operator consisting of mutant and wild-type operator half sites. These results suggest that at least 1 LexA protein monomer interacts with each operator half site. We discuss the role of LexA oligomer formation in binding of LexA to operator DNA. 相似文献
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