P-aminodiphenylamine induced biochemical changes in sex organs of male albino rats.

Intraperitoneal administration of p-aminodiphenylamine (p-ADPA), an aromatic amine of wide industrial applications, / 42.5 mg/kg body weight for 180 days significantly decreased the activities of testicular lactate dehydrogenase and hyaluronidase and lactic acid content indicating arrest of spermatogenesis. Patchy necrosis of the testis was confirmed histopathologically. No change in testicular cholesterol, fructose content of coagulating glands and dorso-lateral prostate and activities of alkaline phosphatase in seminal vesicle and acid phosphatase in ventral prostate support normal androgenic status.     

Dietary hexachlorocyclohexane induced changes in blood and liver lipids in albino mice.

Dietary intake of technical hexachlorocyclohexane (HCH) by albino mice for 2 weeks (at 400 and 800 ppm) resulted in hyperlipemia. Significant increase in triglycerides, phospholipids and cholesterol fractions of blood was observed in these animals. Dietary intake of gamma-isomer of HCH for 2 weeks (at 200 ppm) did not have any effect on blood lipid profile, but at 400 ppm level produced higher contents of phospholipids and cholesterol. The hepatomegaly produced by dietary technical HCH or gamma-HCH was not accompanied by fatty metamorphosis of liver. Hypertriglyceridemia caused by HCH was accompanied by lower triglyceride levels in liver, suggesting a possible higher rate of secretion from liver.     

Correction of changes induced by toluene in the cortical and subcortical structures of albino rat brain

The number and weight of cells in the cortical and subcortical structures of the cerebral and cerebellar motor system in albino rats after a long-term exposure to toluene were determined. Toluene intoxication proved to kill projection neurons and interneurons in the sensorimotor cortex, ventrolateral thalamic nucleus, caudate nucleus, pallidum, red nucleus, and inferior olivary complex. The decreased number of cerebellar cells was mediated by atrophic changes as indicated by the decrease in the area and dry weight of Purkinje cells. The addition of plaferon LB to the diet attenuated the cytotoxic effect of toluene.     

Granulomatous reactions induced by lipids extracted from Fonsecaea pedrosoi, Fonsecaea compactum, Cladosporium carrionii and Phialophora verrucosum

Granulomatous reactions induced by lipid extracts from the dermatophyte fungi Fonsecaea pedrosoi, Fonsecaea compactum, Cladosporium carrionii and Phialophora verrucosum, the causal organisms of chromoblastomycosis, were studied. Charcoal particles coated with the lipid extracts were prepared and injected intravenously into mice. Inflammation was characterized by an intense mononuclear cell infiltrate that lodged in the lung from 4 to 8 d after inoculation.     

Metabolic changes in lipids of rat plasma and hepatocytes induced by 17 alpha-ethynylestradiol treatment

Cultured hepatocytes isolated from livers of 17 alpha-ethynylestradiol-treated rats were used to investigate the change of lipid metabolism induced by administration of 17 alpha-ethynylestradiol. Treatment with 17 alpha-ethynylestradiol caused a decrease of rat plasma lipids (free cholesterol, cholesterol ester, triacylglycerol and phosphatidylcholine). No difference in the ability of urea nitrogen synthesis could be demonstrated between cultured hepatocytes isolated from livers of 17 alpha-ethynylestradiol-treated rats and propylene glycol-treated rats (control). Total cholesterol and cholesterol ester contents of cultured hepatocytes isolated from livers of 17 alpha-ethynylestradiol-treated rats were increased in comparison with those of the control. Triacylglycerol content of cultured hepatocytes was not affected by 17 alpha-ethynylestradiol treatment. There was no difference in the composition of lipid content between liver tissues and cultured hepatocytes. These results suggest that hepatocytes isolated from livers maintain the character of livers treated with 17 alpha-ethynylestradiol or livers treated with propylene glycol. Free cholesterol and cholesterol ester synthesis from [14C]acetic acid by cultured hepatocytes isolated from livers of 17 alpha-ethynylestradiol-treated rats were decreased to about 30% of the control. Triacylglycerol and polar lipid (phospholipid) synthesis from [14C]acetic acid were not affected by 17 alpha-ethynylestradiol treatment. Microsomal hydroxymethylglutaryl-CoA reductase activity of rat liver treated with 17 alpha-ethynylestradiol was decreased to about 50% of control. The secretions of free cholesterol, cholesterol ester, triacylglycerol, phosphatidylcholine, apolipoprotein BL and BS by cultured hepatocytes isolated from livers of 17 alpha-ethynylestradiol treated rats were not decreased when compared with the control. Because lipid and apolipoprotein secretions from cultured hepatocytes treated with 17 alpha-ethynylestradiol were not decreased and cholesterol contents of liver tissues and cultured hepatocytes treated with 17 alpha-ethynylestradiol were increased and hepatic microsomal hydroxymethylglutaryl-CoA reductase activity was decreased by 17 alpha-ethynylestradiol treatment, it is suggested that the liver plays an important role in hypolipidemia induced by 17 alpha-ethynylestradiol by increasing the plasma lipid uptake mediated by an increased amount of lipoprotein receptors of liver membranes.     

Quantitative cytochemical changes induced by dexamethasone and adrenalectomy in rat liver chromatin

Physiochemical changes in the state of chromatin shortly following glucocorticoid stimulation of target cells are predicted by the proposed mechanism of steroid action. These changes had not been previously demonstrated in situ. The present experiments demonstrate that in the intact rat, or in one which has been adrenalectomized but given a moderate dose of dexamethasone, the thermal stability of liver cell chromatin is significantly reduced over the level observed in the adrenalectomized untreated animal. This alteration was rated by measuring nuclear acridine orange metachromasia following chromatin denaturation. These data also show an enhanced binding of the dye by the liver cell nuclei under the same conditions. Feulgen dye binding was also found to be enhanced by dexamethasone stimulation but to a level indicative of configurational changes in the chromatin rather than an increase in the amount of DNA in the cells.     

Amniotic fluid total lipids, lipid phosphorus, phospholipids and cholesterol in diabetic women

Total lipids, lipid phosphorus, phospholipids and cholesterol were determined in amniotic fluid of 36 normals and 6 mothers with diabetes. Total lipids, lipid phosphorus and phospholipids are significantly decreased in diabetic cases and the decrement was more pronounced in the cases with stillbirth or intrapartum deaths or infants with respiratory distress syndrome. Total cholesterol was significantly decreased in the same cases mentioned above. However, in spite of this decrease the ratio of total cholesterol to total lipids was constant except in cases with prolonged intrauterine fetal death where it was increased.     

Growth and aging in the rat: changes in total protein, cellularity, and polyploidy in various organs

The objectives of this study were to determine the influence of growth and aging on ploidy, cell number, and protein content of various organs. Tissue homogenates were prepared at 3, 8, 25, 50, and 100 weeks of age. Samples were analyzed for DNA per nucleus (by flow cytofluorometry), nuclei number, and protein content. Livers of 8- and 100-week-old animals were also perfused with collagenase and the released cells separated into parenchymal and nonparenchymal populations by unit gravity sedimentation. Nuclei of these cells were also analyzed for DNA. In all four zones of the kidney and in thyroid, 4n nuclei diminished in percentage between 3 and 50 weeks and increased at 100 weeks. In the growth phase these probably are cycling cells and after 50 weeks represent an increasing population of nuclei arrested after synthesis of DNA. Constant levels of ploidy were found in brain, heart, rectus abdominis, and adrenal throughout the 3-100 weeks. A dramatic increase in 4n nuclei occurred between 3 and 8 weeks in liver with little change occurring thereafter. Ploidy is a property of only parenchymal cells in liver and this probably is also true in other organs. The 4n nuclei that remain in constant proportion to the total population are established early in life and are not related to aging. They are probably tetraploid and replicate into 4n daughter cells during growth. Cerebrum shows no changes in nuclei number but exhibits a 70% increase in protein between 3 and 100 weeks. Although kidney, liver and adrenal show large increases in number of nuclei (approximately equal to fourfold) with growth, these are not as great as increases in body weight (approximately equal to 11-fold). With regard to organ protein, only liver shows increases approximating those in body weight. Increases in organ nuclei appear to occur in concert for adrenal, kidney, and liver whereas increases in organ protein bear no relationship to each other. Protein content remains at stable levels in organs of 100-week-old animals and little (adrenal, liver) or no (brain, kidney) diminution occurs in nuclei numbers.     

Permeability changes induced by peroxidation in liposomes prepared from human erythrocyte lipids

The diffusion of [2-(14)C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl(2) before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these lipids also resulted in the destruction of polyunsaturated fatty acids and the generation of conjugated dienes, but neither of these processes appeared to be the sole cause of increased glucose efflux. Thin-layer chromatography and the effects of aqueous washes suggested that surface-active lysophosphatides or other lipid degradation products were responsible for the increased permeability of the treated liposomes. It is suggested that the behavior of this liposome model system may be relevant to the high permeability and fragility of vitamin E-deficient erythrocytes.