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Telomere length determined by the fluorescence in situ hybridisation distinguishes malignant and benign cells in cytological specimens 下载免费PDF全文
Y. Matsuda A. Suzuki S. Esaka Y. Hamashima M. Imaizumi M. Kinoshita H. Shirahata Y. Kiso H. Kojima M. Matsukawa Y. Fujii N. Ishikawa J. Aida K. Takubo T. Ishiwata M. Nishimura T. Arai 《Cytopathology》2018,29(3):262-266
Background
Telomeres are tandem repeats of TTAGGG at the end of eukaryotic chromosomes that play a key role in preventing chromosomal instability. The aim of the present study is to determine telomere length using fluorescence in situ hybridisation (FISH) on cytological specimens.Methods
Aspiration samples (n = 41) were smeared on glass slides and used for FISH.Results
Telomere signal intensity was significantly lower in positive cases (cases with malignancy, n = 25) as compared to negative cases (cases without malignancy, n = 16), and the same was observed for centromere intensity. The difference in DAPI intensity was not statistically significant. The ratio of telomere to centromere intensity did not show a significant difference between positive and negative cases. There was no statistical difference in the signal intensities of aspiration samples from ascites or pleural effusion (n = 23) and endoscopic ultrasound‐guided FNA samples from the pancreas (n = 18).Conclusions
The present study revealed that telomere length can be used as an indicator to distinguish malignant and benign cells in cytological specimens. This novel approach may help improve diagnosis for cancer patients. 相似文献2.
Background
Normalization is a basic step in microarray data analysis. A proper normalization procedure ensures that the intensity ratios provide meaningful measures of relative expression values. 相似文献3.
Background
Post-hybridization washing is an essential part of microarray experiments. Both the quality of the experimental washing protocol and adequate consideration of washing in intensity calibration ultimately affect the quality of the expression estimates extracted from the microarray intensities. 相似文献4.
Alexander Glassmann Sabine Molly Lachezar Surchev Tommy A Nazwar Martin Holst Wolfgang Hartmann Stephan L Baader John Oberdick Torsten Pietsch Karl Schilling 《BMC developmental biology》2007,7(1):111
Background
Mtss1 encodes an actin-binding protein, dysregulated in a variety of tumors, that interacts with sonic hedgehog/Gli signaling in epidermal cells. Given the prime importance of this pathway for cerebellar development and tumorigenesis, we assessed expression of Mtss1 in the developing murine cerebellum and human medulloblastoma specimens. 相似文献5.
Annie Glatigny Hervé Delacroix Thomas Tang Nicolas Fran?ois Lawrence Aggerbeck Marie-Hélène Mucchielli-Giorgi 《BMC bioinformatics》2009,10(1):98
Background
There are many sources of variation in dual labelled microarray experiments, including data acquisition and image processing. The final interpretation of experiments strongly relies on the accuracy of the measurement of the signal intensity. For low intensity spots in particular, accurately estimating gene expression variations remains a challenge as signal measurement is, in this case, highly subject to fluctuations. 相似文献6.
Victoriya V Volkova J Allen Byrd Sue Ann Hubbard Danny Magee Richard H Bailey Robert W Wills 《Acta veterinaria Scandinavica》2010,52(1):46
Background
Lighting is used during conventional broiler grow-out to modify bird behaviour to reach the goals of production and improve bird welfare. The protocols for lighting intensity vary. In a field study, we evaluated if the lighting practices impact the burden of Salmonella in broiler flocks. 相似文献7.
Aneta Kasza Paulina Wyrzykowska Irena Horwacik Piotr Tymoszuk Danuta Mizgalska Karren Palmer Hanna Rokita Andrew D Sharrocks Jolanta Jura 《BMC molecular biology》2010,11(1):14
Background
MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. 相似文献8.
Background
The callipyge mutation is located within an imprinted gene cluster on ovine chromosome 18. The callipyge trait exhibits polar overdominant inheritance due to the fact that only heterozygotes inheriting a mutant paternal allele (paternal heterozygotes) have a phenotype of muscle hypertrophy, reduced fat and a more compact skeleton. The mutation is a single A to G transition in an intergenic region that results in the increased expression of several genes within the imprinted cluster without changing their parent-of-origin allele-specific expression. 相似文献9.
Stéphanie Jaubert-Possamai Gaël Le Trionnaire Joël Bonhomme Georges K Christophides Claude Rispe Denis Tagu 《BMC biotechnology》2007,7(1):63
Background
RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. 相似文献10.
Background
Drought is one of the major abiotic stresses affecting plant growth, development and crop productivity. ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes. 相似文献11.
Shin-Young Hong Pil Joon Seo Moon-Sik Yang Fengning Xiang Chung-Mo Park 《BMC plant biology》2008,8(1):112
Background
The wild grass species Brachypodium distachyon (Brachypodium hereafter) is emerging as a new model system for grass crop genomics research and biofuel grass biology. A draft nuclear genome sequence is expected to be publicly available in the near future; an explosion of gene expression studies will undoubtedly follow. Therefore, stable reference genes are necessary to normalize the gene expression data. 相似文献12.
Background
PL10 homologs exist in a wide range of eukaryotes from yeast, plants to animals. They share a DEAD motif and belong to the DEAD-box polypeptide 3 (DDX3) subfamily with a major role in RNA metabolism. The lineage-specific expression patterns and various genomic structures and locations of PL10 homologs indicate these homologs have an interesting evolutionary history. 相似文献13.
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Boris Wilmes Angelika Hartung Michael Lalk Manuel Liebeke Thomas Schweder Peter Neubauer 《Microbial cell factories》2010,9(1):72
Background
Pseudoalteromonas haloplanktis is a cold-adapted γ-proteobacterium isolated from Antarctic sea ice. It is characterized by remarkably high growth rates at low temperatures. P. haloplanktis is one of the model organisms of cold-adapted bacteria and has been suggested as an alternative host for the soluble overproduction of heterologous proteins which tend to form inclusion bodies in established expression hosts. Despite the progress in establishing P. haloplanktis as an alternative expression host the cell densities obtained with this organism, which is unable to use glucose as a carbon source, are still low. Here we present the first fed-batch cultivation strategy for this auspicious alternative expression host. 相似文献15.
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Steven D Fleming Asgerally T Fazleabas Stephen C Bell 《Reproductive biology and endocrinology : RB&E》2008,6(1):6