共查询到20条相似文献,搜索用时 15 毫秒
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A striking similarity in the organization of the E-selectin and beta interferon gene promoters. 总被引:17,自引:6,他引:11
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M Z Whitley D Thanos M A Read T Maniatis T Collins 《Molecular and cellular biology》1994,14(10):6464-6475
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To replicate, a retrovirus must synthesize a cDNA copy of the viral RNA genome and integrate that cDNA into a chromosome of the host. We have investigated the role of a host cell cofactor, HMG I(Y) protein, in integration of human immunodeficiency virus type 1 (HIV-1) and Moloney murine leukemia virus (MoMLV) cDNA. Previously we reported that HMG I(Y) cofractionates with HIV-1 preintegration complexes (PICs) isolated from freshly infected cells. PICs depleted of required components by treatment with high concentrations of salt could be reconstituted by addition of purified HMG I(Y) in vitro. Here we report studies using immunoprecipitation that indicate that HMG I(Y) is associated with MoMLV preintegration complexes. In mechanistic studies, we show for both HIV-1 and MoMLV that each HMG I(Y) monomer must contain multiple DNA binding domains to stimulate integration by HMG I(Y)-depleted PICs. We also find that HMG I(Y) can condense model HIV-1 or MoMLV cDNA in vitro as measured by stimulation of intermolecular ligation. This reaction, like reconstitution of integration, depends on the presence of multiple DNA binding domains in each HMG I(Y) monomer. These data suggest that binding of multivalent HMG I(Y) monomers to multiple cDNA sites compacts retroviral cDNA, thereby promoting formation of active integrase-cDNA complexes. 相似文献
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A novel Ets-related transcription factor, Elf-1, binds to human immunodeficiency virus type 2 regulatory elements that are required for inducible trans activation in T cells. 总被引:8,自引:2,他引:6
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J M Leiden C Y Wang B Petryniak D M Markovitz G J Nabel C B Thompson 《Journal of virology》1992,66(10):5890-5897
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Perrella MA Pellacani A Wiesel P Chin MT Foster LC Ibanez M Hsieh CM Reeves R Yet SF Lee ME 《The Journal of biological chemistry》1999,274(13):9045-9052
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Stimulation of the human immunodeficiency virus type 1 enhancer by the human T-cell leukemia virus type I tax gene product involves the action of inducible cellular proteins. 总被引:25,自引:15,他引:10
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E Bhnlein M Siekevitz D W Ballard J W Lowenthal L Rimsky H Bogrd J Hoffman Y Wano B R Franza W C Greene 《Journal of virology》1989,63(4):1578-1586