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1.
The effect of separate and combined activity of Paecilomyces fumosoroseus Wize (Brown and Smith) Trinidadian strain T11 and the parasitoid, Encarsia formosa Gahan, was assessed on populations of the greenhouse whitefly, Trialeurodes vaporariorum (Westwood), infesting Phaseolus vulgaris L. (French bean) and Pelargonium x domesticum (regal geranium) plants in replicate experiments. When infested bean and geranium plants were exposed to E. formosa for 2 days, and 4 days later sprayed with P. fumosoroseus blastospores, whitefly percent mortality was 99.5% and 75.5%, 94.6% and 59.4% for experiments 1 and 2, respectively. Treatment
of infested bean plants with either E. formosa or P. fumosoroseus resulted in 87.8% and 78.7%, 73.1% and 97.0% whitefly mortality for experiments 1 and 2, respectively, while similar treatment
of infested geranium plants resulted in 9.2% and 52.8%, 34.3% and 64.5% whitefly mortality for experiments 1 and 2, respectively.
Our results support the use of E. formosa and P. fumosoroseus in combination in Experiment 1 for the treatment of whitefly infested P. vulgaris plants since a significant difference in mortality is observed than when either E. formosa or P. fumosoroseus is applied alone. However, in experiment 2, the combination treatment on P. vulgaris was no more effective than spraying P. fumosoroseus alone. On P. x domesticum plants, only P. fumosoroseus alone is needed for efficient control of the whitefly compared to the combination treatment. The relative timing of parasitoid
oviposition and fungal infection are critical in determining the outcome of the interaction and are plant host dependent. 相似文献
2.
This work aimed to study the biology of Clitostethus arcuatus (Rossi) (Coleoptera: Coccinellidae) under different temperatures and evaluate the optimum temperature for its mass rearing.
Studies were carried out in the laboratory at four constant temperatures (15°C, 20°C, 25°C and 30°C), 75 ± 5% relative humidity
and a photoperiod of 16 h light:8 h dark, in which C. arcuatus was fed ad libitum with nymphs of all instar of Aleyrodes proletella L. (Homoptera: Aleyrodidae) on Brassicae oleracea L. (var. Costata). The following biological parameters were evaluated: development time and survival rates of pre-imaginal stages, adult longevity
(female and male), length of the pre-oviposition and oviposition periods, fecundity, fertility and percentage of egg hatching.
Population growth parameters, the lower development threshold and the sum of effective temperatures were estimated. Temperatures
ranging from 20°C to 30°C were suitable for the development of C. arcuatus, suggesting that this species is well adapted to the temperatures usually found inside greenhouses or in open fields in temperate
regions. Although the intrinsic rate of natural increase and doubling time were similar at 25°C and 30°C, the temperature
of 25°C was shown to be the most suitable for mass rearing and development of populations under field conditions, since the
percentage of egg hatching and the accumulated survival rates of the pre-imaginal stages were the highest. Considering the
estimated lower threshold for pre-imaginal development (7.9°C) and the sum of effective temperatures [293.6 degree-days (°D)],
it is predicted for Ponta Delgada (Azores, Portugal) that the first adults of C. arcuatus should emerge by the first fortnight of February and that up to 12 generations per year can occur. 相似文献
3.
The seasonal occurrence of Mycosphaerella buna in leaves and contiguous organs of Fagus crenata was studied in a Japanese beech forest, Ibaraki, Japan, in 1998 and 1999. Mycosphaerella buna was not isolated from newly developed leaves in May, but it was isolated from asymptomatic leaves after June. The frequency of its occurrence gradually increased until leaffall. In contrast, M. buna was not isolated from overwintered buds, leaf petioles, or contiguous current-year twigs. The spermogonia and pseudothecia were observed in dead leaves after leaffall. The mature pseudothecia were found on dead leaves from May to July. The ascospores produced in the pseudothecia were suggested to infect newly developed leaves.Contribution no. 173, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba, Japan 相似文献
4.
Gaye Öngen Gaye Güngör Bahar Kanberoglu 《World journal of microbiology & biotechnology》2007,23(4):519-524
Aspergillus section Nigri strains Aspergillus aculeatus Ege-K 258, A.
foeditus var. pallidus Ege-K156, A. niger Ege-K 4 and A. tubingensis Ege-K 265 were used to treat olive mill wastewater (OMW) in an investigation aimed at exploring their dephenolisation and
decolourisation ability and, consequently, the economic feasibility of using any or all of these strains in a pre-treatment
step in the processing of OMW. Of these strains A. tubingensis Ege-K 265 resulted in an 80% decolourisation of twofold-diluted OMW and a 30% decolourisation of undiluted OMW; in addition,
it was able to remove approximately 30% of all phenolic compounds in both twofold-diluted and undiluted OMW. We conclude that
A. tubingensis Ege-K 265 could be effectively used in the pre-treatment step of a combined aerobic-anaerobic process to solve the environmental
problems caused by OMW in Mediterranean countries. 相似文献
5.
6.
S. E. Dawson 《Kew Bulletin》2008,63(3):517-517
Summary The name Pavetta modesta (Hiern) S. E. Dawson is a later homonym of P. modesta Bremek. Pavetta crystalensis is proposed as a new name. 相似文献
7.
Septobasidium parviflorae sp. nov. on Pinus parviflora is described and illustrated. This species is characterized by its whitish-gray, gray to dark gray-colored fungus body with
an indeterminate margin, hyphal strands, and cylindrical basidia with long sterigmata. This is the first report of Septobasidium occurring on a member of the genus Pinus in Japan. 相似文献
8.
Tarun Kumar Verinder Wahla Piyush Pandey R. C. Dubey D. K. Maheshwari 《World journal of microbiology & biotechnology》2009,25(2):277-285
Biological control of the cyst forming nematode Heterodera cajani was studied on sesame using plant growth promoting rhizobacteria (PGPR) Pseudomonas aeruginosa LPT3 and LPT5. Based on plant growth promoting attributes, two fluorescent pseudomonads, LPT3 and LPT5 were evaluated for
their efficacy against cyst forming nematode Heterodera cajani that parasitize Sesamum indicum. Pseudomonas aeruginosa LPT5 produced IAA, HCN, chitinase, glucanase and siderophore, and also solubilized inorganic phosphate in vitro. Moreover,
LPT5 resulted in mortality of second stage juveniles of H. cajani, which was 13% higher as compared to P. aeruginosa LPT3. Interestingly, when both strains were inoculated together for the management of H. cajani on Sesamum indicum the population of H. cajani was reduced significantly, in field trial. Approximately 60% reduction in cyst and juveniles population was recorded with
LPT5 coated seeds, while LPT3 resulted in 49% reduction in cyst and juvenile population as compared to control. Plants grown
with seeds bacterized with LPT5 and reduced doses of urea, diammonium phosphate (DAP), muriate of potash (K) and gypsum gave
maximum increase in yield, in comparison to that of plants raised under the influence of recommended or full doses of the
chemical fertilizers. Pseudomonas aeruginosa LPT5 also showed excellent root colonization. 相似文献
9.
The architecture of the musculature of the eutardigrade species Milnesium tardigradum Doyère, 1840, Hypsibius sp. and Ramazzottius oberhaeuseri (Doyère in Ann Sci Nat Zool Sér 2(14):269–369, 1840) is investigated by phalloidin staining and confocal laser scanning microscopy. There are methodological problems in staining
eutardigrades due to physiological alterations under stress (anhydrobiosis) and due to penetration problems of the cuticle.
It is helpful to fix specimens in the state of asphyxy, where animals are stretched following an oxygen shortage in their
environment. The musculatures of all three species correspond in their general architecture, but differ in detail, such as
in the number of muscles. All muscles are isolated muscle strands. There are on each body side two dorsal and one ventral
muscle strands, in addition to a system of dorsoventral, lateral and lateroventral muscles. Seven median ventral attachment
points give rise to dorsoventral, ventrolateral and appendage muscles. The appendages receive several muscles originating
dorsally and ventrally. The number of muscles and the arrangement differ in each appendage. The fourth appendage shows the
greatest differences with a far smaller number of muscles compared to other species. The musculature shows comparably few
strict segmental patterns, for example, the musculature of each appendage differs from the other ones. By comparison with
literature data on the same species and data of Macrobiotus hufelandi it can be shown that eutardigrades have a roughly comparable muscular architecture, but that there are several differences
in detail.
Dedicated to Professor Westheide on the occasion of his 70th birthday. 相似文献
10.
Peter G. Wilson 《Kew Bulletin》2008,63(2):347-349
Summary The type specimen of Metrosideros regelii is discussed. It contains a mixture of two species, representing different genera, and a lectotype is chosen. The generic
position of the species is considered in the light of morphology and recent molecular evidence and the new combination, Mearnsia regelii, made. 相似文献
11.
S. Kumar Sunil Kumar S. P. Negi J. K. Kanwar 《In vitro cellular & developmental biology. Plant》2008,44(6):474-479
Callus cultures derived from leaf segments of chrysanthemum cultivar ‘Snow Ball’ which was susceptible to Septoria obesa were successfully used for in vitro selection for resistance to this pathogenic fungus. Resistant cell lines were selected by culturing callus on growth medium
containing various concentrations of S. obesa filtrate. Resistant calluses obtained after two cycles (30 d each cycle) of selection were used for plant regeneration. About
30% of the plants regenerated from the resistant calluses and 70–80% of the plants raised from cuttings had acquired considerable
resistance against the pathogen in the field. No phenotypic variation was observed in the selected regenerates. 相似文献
12.
Indeok Hwang Dilli Prasad Paudyal Seong-Ki Kim Hyeonsook Cheong 《Biotechnology and Bioprocess Engineering》2007,12(2):157-164
Inhibitors are very important in the study of hormone function. Brasinazole (Brz) is a specific inhibitor of brassinosteroids
(BRs) biosynthesis. To expand our knowledge of the molecular mechanisms of plant steroid signaling, we performed genetic screening
using medium containing Brz under dark conditions. Mutants insensitive to Brz developlonger hypocotyls than their wild type
counterparts. We isolatedabz453 as a Brz insensitive mutant. TAIL-PCR and the segregation ratio of T2 plants indicated a single T-DNA insertion at the 24-Sterol
C-methyltransferase (SMT2) gene in theabz453 mutant. Recapitulation for putative FCP serine phosphatase (FSP), the gene neighboringSMT2, indicated no significant phenotypes, but theSMT2 anti-sense (SMT2-AS) line developed longer hypocotyls than the wild type in medium containing Brz. Additionally, theSMT2-AS line displayed similar phenotypes to theabz453 line in soil including enhanced growth and smaller silique. Theabz453 andSMT2-AS mutants showed phenotypes similar to those of wild type in medium containing benzylaminopurine, pacrobutrazol and ACC (precursor
for ethylene) under dark conditions. However, when brassinolide (BL) dose response was observed, theabz453 andSMT2-AS lines showed higher sensitivity than wild type. Theabz453/det2 andabz453/bri1-119 double mutants showed enhanced growth compared to thedet2 andbri1-119 line under both dark and light conditions. Specially, in dark conditions double mutants displayed nearly 2- and 1.5-fold
longer hypocotyls thandet2 andbri1-119 plants. Brz insensitivity to theSMT2 knock-out mutant and phenotypes of double mutants indicate that not only do BRI1 and DET2 influence the BRs response, as
evidenced by hypocotyl elongation, but another sterol derived signals may also be affected in mutants, suggesting that another
pathway is involved in hypocotyl elongation due to SMT2. 相似文献
13.
The research on incidence of mistletoe (Viscum album ssp. abietis) on silver fir (Abies alba) was carried out in natural fir stands in Croatia. In the area of Gorski Kotar 32.8 % of the examined dominant fir trees
were infected with mistletoe. The mistletoe incidence was presented according to the damage degrees of silver fir from 0 (healthy
trees) to 4 (dead trees), rated by the crown defoliation and needle discoloration. With the increase of incidence, mistletoe
spreads more onto more vital, i.e. less damaged trees. In the Dinaric Alps 28.6% of the examined fir trees were infected,
this percentage amounting to 27.1% for the mountainous regions between the Sava and Drava rivers.
The site and stand parameters (exposure, elevation, site quality, forest community and crown closure) were analysed in order
to establish whether there was a correlation between these parameters and mistletoe incidence. There was a negative correlation
between the elevation and mistletoe incidence. Among the compartments with a closed stands there were considerably less compartments
with higher mistletoe incidence than among compartments with a sparsely closed and understocked stands. The other analysed
site and stand parameters individually had no significant influence on mistletoe incidence.
The correlation of silver fir mortality in 2004 and mistletoe incidence in 2002/03 was analysed, and their strong correlation
was established. Mistletoe could be considered as a bioindicator of silver fir decline, and probably a significant contributor
to that decline. In the areas where mistletoe incidence is great it can be presumed that silver fir is significantly damaged. 相似文献
14.
Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds
on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which
is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity
is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin
A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM,
has been expressed. 相似文献
15.
We studied the oviposition performance of Fopius arisanus (Sonan) (Hymenoptera: Braconidae) attacking eggs of four fruit flies of the genus Anastrepha Schiner (Diptera: Tephritidae) under laboratory conditions. The complete process of oviposition on an individual egg of Anastrepha ludens (Loew) lasts in average 85.4 ± 2.9 s, including a tremor (25.8 ± 1.03 s) observed in the middle of this process related to
the egg’s descent. The average parasitism of A. ludens egg was 60.9 ± 7.5%, with only 1.2% of superparasitized eggs. During individual acts of oviposition, we noted that F. arisanus possesses a highly flexible ovipositor that curves easily as it searches for additional suitable eggs, which may be of particular
benefit when a female finds large clutches of eggs. The individual oviposition of F. arisanus in host fruits attacked by Anastrepha spp. varies with the egg clutch size of each fruit fly species: A. serpentina laid the biggest egg clutches (21.3 ± 1.4), followed by A. ludens (14.2 ± 0.9), and A. striata (1.0 ± 0.0) (=A. obliqua). The time spent by F. arisanus in individual ovipositions was parallel to these findings, reinforcing the idea that F. arisanus attacks several eggs in each individual insertion of its ovipositor. Neither formal oviposition acts, nor adult emergences
of F. arisanus were registered in A. obliqua. We discuss the potential of F. arisanus as natural enemy of fruit flies of the genus Anastrepha, and explore the eventual developing of its mass rearing.
Handling Editor: Torsten Meiners. 相似文献
16.
Dae-Sung Lee Min-Seung Kang Hye-Jin Hwang Sung-Hwan Eom Ji-Young Yang Myung-Suk Lee Won-Jae Lee You-Jin Jeon Jae-Sue Choi Young-Mog Kim 《Biotechnology and Bioprocess Engineering》2008,13(6):758-764
We have been attempting for some time to discover a compound evidencing antibacterial activity against methicillin-resistant
Staphylococcus aureus (MRSA). The dieckol isolated from Ecklonia stolonifera has been shown to exhibit antibacterial activity against methicillin-susceptible S. aureus (MSSA) and MRSA. The minimum inhibitory concentrations (MICs) of dieckol were determined in a range of 32 to 64 μg/mL against
standard MSSA and MRSA strains. Furthermore, dieckol clearly reversed the high-level ampicillin and penicillin resistance
of MRSA. The MICs of ampicillin against two standard strains of MRSA were dramatically reduced from 512 to 0.5 μg/mL in combination
with 1/4 MIC of dieckol (16 μg/mL). The fractional inhibitory concentration (FIC) indices of ampicillin and penicillin were
measured from 0.066 to 0.266 in combination with 8 or 16 μg/mL of dieckol against all tested MRSA strains, thereby suggesting
that dieckol-ampicillin or dieckol-penicillin combinations exert a synergistic effect against MRSA. The results of this study
indicate that dieckol, administered in combination with β-lactams, may prove effective in the treatment of MRSA infections.
Our finding may also contribute to the development of an alternative phytotherapeutic anti-MRSA agent. 相似文献
17.
L. Y. Solís-Ramos T. González-Estrada S. Nahuath-Dzib L. C. Zapata-Rodriguez E. Castaño 《Plant Cell, Tissue and Organ Culture》2009,96(3):279-287
Capsicum chinense is a recalcitrant species for in vitro morphogenesis, and up to date there is no efficient system for genetic transformation
and regeneration of this species via somatic embryogenesis. Here, we carried out an in vitro transformation of C. chinense via Agrobacterium tumefaciens co-cultivation with a system that expresses the heterologous gene WUSCHEL from Arabidopsis thaliana. WUSCHEL has been shown to promote the transition from vegetative to embryogenic state when overexpressed. We tested if the expression
of WUSCHEL in C. chinense would promote an embryogenic response in this species. After 15 days of induction, the segments of transformed stems begun
to form globular structures, suggesting that heterologus WUSCHEL was active and involved in the process of morphogenesis. 相似文献
18.
Dmitar Lakušić 《Biologia》2008,63(5):631-636
The south-European Cardamine plumieri Vill. (sect. Pteroneurum subsect. Cryptopterum) is confirmed for the Flora of Serbia. Several populations were discovered in the central and western part of country, exclusively
on the serpentine bedrocks. Morphological, chorological and ecological characteristics of C. plumieri was studied. 相似文献
19.
20.
In this study, the Phanerotomini of Turkey collected between July 1959 and July 2004, are listed. A total of thirteen Phanerotomini
species were determined, and six of these are reported for the first time from Turkey. Phanerotoma (Bracotritoma) permixtellae Fischer, 1968 was reared from a new natural host Recurvaria pistaciicola (Danilevskii, 1955) (Lepidoptera: Gelechiidae). With this present study an illustrated identification key for the Phanerotomini
of Turkey is given. The distribution of the Phanerotomini species is discussed according to the phytogeographical provinces
of Turkey. 相似文献