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Abstract Seven novel oligonucleotide primer pairs for polymerase chain reaction amplification of introns from nuclear genes
in coelomates were designed and tested. Each pair bound to adjacent exons that are separated by a single intron in most coelomate
species. The primer sets amplified introns in species as widely separated by the course of evolution as oysters (Mollusca:
Protostoma) and salmon (Chordata: Deuterostoma). Each primer set was tested on a further 6 coelomate species and found to
amplify introns in most cases. These primer sets may therefore be useful tools for developing nuclear DNA markers in diverse
coelomate species for studies of population genetics, phylogenetics, or genome mapping. 相似文献
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V. I. Kulinsky L. S. Kolesnichenko 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2009,3(2):129-144
During the last 10–15 years significant progress has been achieved in all directions of studies of the glutathione system. A series of new enzymes involved into metabolism of glutathione has been discovered. Many of these enzymes are polyfunctional and their new activities have been recognized. The enzymes interact with hormones and signal transduction systems. Significant progress has been achieved in the studies of intracellular, intercellular and inter-organ transport. The important achievement is employment of not only selective compounds-analyzers but also gene engineering methods for identification of new functions. 相似文献
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Clare H. Robinson 《Journal of Ecology》2001,89(6):1096-1097
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