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DCL2‐ and RDR6‐dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over‐accumulation
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Yu‐Yi Wu Bo‐Han Hou Wen‐Chi Lee Shin‐Hua Lu Chen‐Jui Yang Hervé Vaucheret Ho‐Ming Chen 《The Plant journal : for cell and molecular biology》2017,90(6):1064-1078
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Arabidopsis ammonium transporters,AtAMT1;1 and AtAMT1;2, have different biochemical properties and functional roles 总被引:5,自引:0,他引:5
Shelden Megan C. Dong Bei de Bruxelles Guy L. Trevaskis Ben Whelan Jim Ryan Peter R. Howitt Susan M. Udvardi Michael K. 《Plant and Soil》2001,231(1):151-160
We have compared the biochemical properties of two different Arabidopsis ammonium transporters, AtAMT1;1 and AtAMT1;2, expressed in yeast, with the biophysical properties of ammonium transport in planta. Expression of the AtAMT1;1 gene in Arabidopsis roots increased approximately four-fold in response to nitrogen deprivation. This coincided with a similar increase in high-affinity ammonium uptake by these plants. The biophysical characteristics of this high-affinity system (Km for ammonium and methylammonium of 8 M and 31 M, respectively) matched those of AtAMT1;1 expressed in yeast (Km for methylammonium of 32 M and Ki for ammonium of 1–10 M). The same transport system was present, although less active, in nitrate-fed roots. Ammonium-fed plants exhibited the lowest rates of ammonium uptake and appeared to deploy a different transporter (Km for ammonium of 46 M). Expression of AtAMT1;2 in roots was insensitive to changes in nitrogen nutrition. In contrast to AtAMT1;1, AtAMT1;2 expressed in yeast exhibited biphasic kinetics for methylammonium uptake: in addition to a high-affinity phase with a Km of 36 M, a low-affinity phase with a Km for methylammonium of 3.0 mM was measured. Despite the presence of a putative chloroplast transit peptide in AtAMT1;2, the protein was not imported into chloroplasts in vitro. The electrophysiological data for roots, together with the biochemical properties of AtAMT1;1 and Northern blot analysis indicate a pre-eminent role for AtAMT1;1 in ammonium uptake across the plasma membrane of nitrate-fed and nitrogen-deprived root cells. 相似文献
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Joseph M. Devaney Laura L. Tosi David T. Fritz Heather A. Gordish‐Dressman Shan Jiang Funda E. Orkunoglu‐Suer Andrew H. Gordon Brennan T. Harmon Paul D. Thompson Priscilla M. Clarkson Theodore J. Angelopoulos Paul M. Gordon Niall M. Moyna Linda S. Pescatello Paul S. Visich Robert F. Zoeller Cinzia Brandoli Eric P. Hoffman Melissa B. Rogers 《Journal of cellular biochemistry》2009,107(6):1073-1082
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P. Matt M. Geiger P. Walch‐Liu C. Engels A. Krapp M. Stitt 《Plant, cell & environment》2001,24(11):1119-1137