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Change of function of the wheat stress‐responsive transcriptional repressor TaRAP2.1L by repressor motif modification
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Amritha Amalraj Sukanya Luang Manoj Yadav Kumar Pradeep Sornaraj Omid Eini Nataliya Kovalchuk Natalia Bazanova Yuan Li Nannan Yang Serik Eliby Peter Langridge Maria Hrmova Sergiy Lopato 《Plant biotechnology journal》2016,14(2):820-832
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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression
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Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518
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Nataliya Kovalchuk Wei Jia Omid Eini Sarah Morran Tatiana Pyvovarenko Stephen Fletcher Natalia Bazanova John Harris Kontanze Beck‐Oldach Yuri Shavrukov Peter Langridge Sergiy Lopato 《Plant biotechnology journal》2013,11(6):659-670
Constitutive over‐expression of the TaDREB3 gene in barley improved frost tolerance of transgenic plants at the vegetative stage of plant development, but leads to stunted phenotypes and 3‐ to 6‐week delays in flowering compared to control plants. In this work, two cold‐inducible promoters with contrasting properties, the WRKY71 gene promoter from rice and the Cor39 gene promoter from durum wheat, were applied to optimize expression of TaDREB3. The aim of the work was to increase plant frost tolerance and to decrease or prevent negative developmental phenotypes observed during constitutive expression of TaDREB3. The OsWRKY71 and TdCor39 promoters had low‐to‐moderate basal activity and were activated by cold treatment in leaves, stems and developing spikes of transgenic barley and rice. Expression of the TaDREB3 gene, driven by either of the tested promoters, led to a significant improvement in frost tolerance. The presence of the functional TaDREB3 protein in transgenic plants was confirmed by the detection of strong up‐regulation of cold‐responsive target genes. The OsWRKY71 promoter–driven TaDREB3 provides stronger activation of the same target genes than the TdCor39 promoter. Analysis of the development of transgenic plants in the absence of stress revealed small or no differences in plant characteristics and grain yield compared with wild‐type plants. The WRKY71–TaDREB3 promoter–transgene combination appears to be a promising tool for the enhancement of cold and frost tolerance in crop plants but field evaluation will be needed to confirm that negative development phenotypes have been controlled. 相似文献
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Special trends in CBF and DREB2 groups in Eucalyptus gunnii vs Eucalyptus grandis suggest that CBF are master players in the trade‐off between growth and stress resistance
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Hong C. Nguyen Phi B. Cao Hélène San Clemente Raphaël Ployet Fabien Mounet Nathalie Ladouce Luc Harvengt Christiane Marque Chantal Teulieres 《Physiologia plantarum》2017,159(4):445-467
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Overexpression of the class I homeodomain transcription factor TaHDZipI‐5 increases drought and frost tolerance in transgenic wheat
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Yunfei Yang Sukanya Luang John Harris Matteo Riboni Yuan Li Natalia Bazanova Maria Hrmova Stephan Haefele Nataliya Kovalchuk Sergiy Lopato 《Plant biotechnology journal》2018,16(6):1227-1240
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Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
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Madoka Kudo Satoshi Kidokoro Takuya Yoshida Junya Mizoi Daisuke Todaka Alisdair R. Fernie Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《Plant biotechnology journal》2017,15(4):458-471
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Anther culture–derived haploid embryos were used as explants for Agrobacterium‐mediated genetic transformation of bread wheat (Triticum aestivum L. cv CPAN1676) using barley HVA1 gene for drought tolerance. Regenerated plantlets were checked for transgene integration in T0 generation, and positive transgenic haploid plants were doubled by colchicine treatment. Stable transgenic doubled haploid plants were obtained, and transgene expression was monitored till T4 generation, and no transgene silencing was observed over the generations. Doubled haploid transgenic plants have faster seed germination and seedling establishment and show better drought tolerance in comparison with nontransgenic, doubled haploid plants, as measured by per cent germination, seedling growth and biomass accumulation. Physiological evaluation for abiotic stress by assessing nitrate reductase enzyme activity and plant yield under post‐anthesis water limitation revealed a better tolerance of the transgenics over the wild type. This is the first report on the production of double haploid transgenic wheat through anther culture technique in a commercial cultivar for a desirable trait. This method would also be useful in functional genomics of wheat and other allopolyploids of agronomic importance. 相似文献