Reconstruction of strings past

A major use of string–alignment algorithms is to comparemacro molecules that are thought to have evolved from a commonancestor to estimate the duration of, or the amount of mutationin, their separate evolution and to infer as much as possibleabout their most recent common ancestor. Minimum message lengthencoding, a method of inductive inference, is applied to thestnng-oJignment pmblem. it leads to an alignment method thataverages over all alignments in a weighted fashion. Esperimentsindicates that this method can recover the actual parametersof evolution with high accuracy and over a wide range of values,whereas the use of a single optimal alignment gives biased results.     

Chromium in the environment: factors affecting biological remediation

Chromium, in the trivalent form (Cr(III)), is an important component of a balanced human and animal diet and its deficiency causes disturbance to the glucose and lipids metabolism in humans and animals. In contrast, hexavalent Cr (Cr(VI)) is highly toxic carcinogen and may cause death to animals and humans if ingested in large doses. Recently, concern about Cr as an environmental pollutant has been escalating due to its build up to toxic levels in the environment as a result of various industrial and agricultural activities. In this review, we present the state of knowledge about chromium mobility and distribution in the environment and the physiological responses of plants to Cr with the desire to understand how these processes influence our ability to use low cost, environmentally friendly biological remediation technologies to clean up Cr-contaminated soils, sediments, and waters. The use of biological remediation technologies such as bioremediation and phytoremediation for the cleanup of Cr-contaminated areas has received increasing interest from researchers worldwide. Several methods have been suggested and experimentally tested with varying degrees of success.     

The biological activity of diuretic factors in Rhodnius prolixus

The rapid post-feeding diuresis of Rhodnius prolixus is under neurohormonal control and involves the integrated activity of the crop, Malpighian tubules and hindgut. One of the factors which is involved in this rapid diuresis is serotonin, however a peptide(s) is also considered to be involved. In other insects, corticotropin releasing factor (CRF)-like and kinin-like, calcitonin-like peptides and CAP(2b) have been demonstrated to be diuretic factors/hormones.In the present study, serotonin and CRF-like peptides increased secretion rate and cAMP content of Rhodnius Malpighian tubules, while the kinin-like peptides tested did not increase secretion rate or cAMP content of the tubules. Extracts of the CNS were processed and several HPLC fractions revealed kinin-like immunoreactivity but these fractions did not increase secretion rate when tested on Malpighian tubules. However, these same fractions did possess activity when tested on the hindgut contraction assay. In addition, material eluting at higher acetonitrile concentrations from the HPLC increased secretion and cAMP content of Rhodnius Malpighian tubules. This material eluted at concentrations of acetonitrile consistent with the elution time of CRF-like peptide standards.Synergism was demonstrated using the pharmacological agent forskolin and serotonin, tested on the rate of secretion of Rhodnius Malpighian tubules, in agreement with data of Maddrell et al. As well, synergism could be demonstrated using mesothoracic ganglionic mass (MTGM) homogenates and serotonin at some concentrations of serotonin. However, combinations of CRF-like material and serotonin increased secretion additively, not synergistically. Kinin-like peptides, tested along with CRF-like material and serotonin, at low concentrations, did not increase secretion above that of those factors tested alone.     

Chemical and biological factors affecting mutagen potency

This review surveys the chemical and biological factors that are correlated with the mutagenic activity of the aromatic and heterocyclic amines. Particular attention is given to the predicted quantum chemical properties of the parent amines and their metabolites. A number of chemical properties have been found to correlate well with measured mutagenic potency, including log P, the enrgies of the frontier orbitals of the parent amines, and the thermodynamic stability of the nitrenium ion, possibly the ultimate DNA-binding species. These correlations are intriguing clues to the mutagenic activity of the aromatic amines; however, many factors still await final explanation, including the exact mechanisms of the metabolic enzymes, the identity(s) of the ultimate DNA-binding species, the reaction mechanism in the DNA-adduction, the role of sequence context in the covalent and non-covalent binding of the adducts, and the role of DNA repair.     

Effect of some biological factors on soil variability in the tropics

Summary Field observations were carried out on an Egbeda soil series in western Nigeria to study the degree of soil-nutrient variability within and between plots due to pre-clearing vegetation. The soil showed lower soil pH, organic C, exchangeable K and Ca contents following cassava grown by traditional methods than following secondary forest vegetation or thicket regrowth. Except for exchangeable K, the degree of variability of the above parameters was observed to be in the following order: secondary forest vegetation > thicket regrowth > cassava plots. The available P status was generally low and showed little relation to pre-clearing vegetation. The implications of soil variability due to pre-clearing vegetation on soil sampling and in field experimentation are discussed. re]19760204     

Effect of some biological factors on soil variability in the tropics

Summary Observations of a selected area in the forest zone of south western Nigeria indicate that the distribution ofMacrotermes bellicosus (Smeathman) andMacrotermes subhyalinus (Rambur) mounds are mainly affected by the drainage conditions of the soils along a toposequence. Higher number of mounds are observed on the better drained soils, ranging from 35.4 to 17.2 mounds/ha covering an area of respectively 0.84 and 0.10 per cent. The shallow and poorly drained soils show lower mound density (<1 mound/ha). The mound soil bears close resemblance to the subsoil, has higher clay content, lower pH, organic C, CEC and extractable P, but higher P retentio than the adjacent surface soil. Growth of maize (Zea mays L.) and soybean (Glycine max. (L.) Merrill) was poorer in the mound soil than in the surface soil. The problem of increased soil variability when large number of mounds are encountered in the field is discussed.