The neuroethology of C. elegans escape

Escape behaviors are crucial to survive predator encounters. Touch to the head of Caenorhabditis elegans induces an escape response where the animal rapidly backs away from the stimulus and suppresses foraging head movements. The coordination of head and body movements facilitates escape from predacious fungi that cohabitate with nematodes in organic debris. An appreciation of the natural habitat of laboratory organisms, like C. elegans, enables a comprehensive neuroethological analysis of behavior. In this review we discuss the neuronal mechanisms and the ecological significance of the C. elegans touch response.     

Comparison of several traps for catching German cockroaches (Dictyoptera: Blattellidae) under laboratory conditions

German cockroach, Blattella germanica (L.) (Dictyoptera: Blattellidae), catch by five types of traps and modifications of each, were tested under controlled laboratory conditions. Cockroach catch differed significantly among traps. Lo-line trap caught the greatest number of cockroaches in the test arena for each size class (23% small nymphs, 39% of gravid females, and 60% of other size classes in the experimental arena). Jar traps caught the least number of cockroaches in the test arena for each size class (range, 7-23% of each size class trapped). Modifications of traps also altered catch of cockroaches. Food bait tablets increased catch significantly; however, increases were small (<10%). Size of traps did not affect catch; whole traps or half traps caught the same number of cockroaches. Jar traps were much less effective than sticky traps, catching only half the number of cockroaches as sticky traps. A thin layer of petrolatum was a more effective barrier in jar traps to cockroach escape than powdered Olancha clay. Traps with petrolatum caught about twice as many cockroaches as traps with clay. Trapping of any of six life stages was not significantly affected by catch of any of the other stages. Rather, trap catch of each life stage was dependent on the number of that life stage available in the experimental arenas. In conclusion, of the traps tested, the Lo-line trap was the most sensitive for measuring cockroach catch, whereas the Detector trap (one third of trap) was the most economical trap (greatest sensitivity for lowest cost).     

Lateral facilitation between primary mechanosensory neurons controls nose touch perception in C. elegans

The nematode C. elegans senses head and nose touch using multiple classes of mechanoreceptor neurons that are electrically coupled through a network of gap junctions. Using in?vivo neuroimaging, we have found that multidendritic nociceptors in the head respond to harsh touch throughout their receptive field but respond to gentle touch only at the tip of the nose. Whereas the harsh touch response depends solely on cell-autonomous mechanosensory channels, gentle nose touch responses require facilitation by additional nose touch mechanoreceptors, which couple electrically to the nociceptors in a hub-and-spoke gap junction network. Conversely, nociceptor activity indirectly facilitates activation of the nose touch neurons, demonstrating that information flow across the network is bidirectional. Thus, a simple gap-junction circuit acts as a coincidence detector that allows primary sensory neurons to integrate information from neighboring mechanoreceptors and generate somatosensory perception.     

An analysis of the selection of small African mammals by two break-back traps

The records of animals caught by two kinds of breakback trap (Reporter and Museum Special) have been analysed to investigate the ways in which the catch of a trap may be selective. The data comprise 1583 individuals belonging to 12 species of small mammal.
The Reporter trap tends, in two distinct ways, to catch individuals which are heavier. Firstly, the proportion of the total catch of a given species caught by Reporter increases with the mean weight of the species. Secondly, within each species (with one trivial exception) the individuals caught by Reporter are, on average, heavier, the mean difference being 36%. In both of these respects significant species x trap interactions remain after the effect of species mean weight have been corrected for.     

Assessment of a silhouette trap for sampling zoophilic blackflies (Diptera: Simuliidae)

A collapsible animal-type silhouette trap was designed to catch zoophilic female blackflies (Diptera: Simuliidae) which feed on the head or ventral surface of cattle. The trap was assessed for its ability to sample attacking blackflies by comparison with simultaneous manual catches from a bait cow, in an enzootic bovine onchocerciasis area in North Wales. In thirteen 1-hour collections, the trap provided a representative sample of the blackfly population in terms of the relative abundance of species. There was a strong correlation between the catch sizes from both methods (r = +0.73), and the body site feeding preferences for all species were similar with both trap and cow. There was no significant difference between the Onchocerca spp. infection rates of flies caught by either method.     

Tyramine Functions independently of octopamine in the Caenorhabditis elegans nervous system

Octopamine biosynthesis requires tyrosine decarboxylase to convert tyrosine into tyramine and tyramine beta-hydroxylase to convert tyramine into octopamine. We identified and characterized a Caenorhabditis elegans tyrosine decarboxylase gene, tdc-1, and a tyramine beta-hydroxylase gene, tbh-1. The TBH-1 protein is expressed in a subset of TDC-1-expressing cells, indicating that C. elegans has tyraminergic cells that are distinct from its octopaminergic cells. tdc-1 mutants have behavioral defects not shared by tbh-1 mutants. We show that tyramine plays a specific role in the inhibition of egg laying, the modulation of reversal behavior, and the suppression of head oscillations in response to anterior touch. We propose a model for the neural circuit that coordinates locomotion and head oscillations in response to anterior touch. Our findings establish tyramine as a neurotransmitter in C. elegans, and we suggest that tyramine is a genuine neurotransmitter in other invertebrates and possibly in vertebrates as well.     

Behavioural interactions between the lizard <Emphasis Type="Italic">Takydromus tachydromoides</Emphasis> and the praying mantis <Emphasis Type="Italic">Tenodera aridifolia</Emphasis> suggest reciprocal predation between them

The Japanese lacertid lizard Takydromus tachydromoides and the praying mantis Tenodera aridifolia are sympatric generalist predators feeding on similar prey. To confirm reciprocal predation between them, we observed the behavioural interactions between the lizards and the mantises of different sizes in a laboratory condition. The lizards caught small mantises (from first to fifth instars), but sometimes escaped from large mantises (from sixth instar to adult). Large mantises occasionally showed catch responses to the lizards. The lizards sometimes caught the mantis without a tongue-flick response (sampling of chemical cues), and they sometimes did not catch the small mantises showing immobile or cryptic responses that prevent visual detection. These results suggested the primary role of vision on recognition of the mantis as a prey. The lizards spent a longer time to approach larger mantises. The time from orienting to catch was longer when the lizards showed tongue-flick responses. The lizard also spent a longer time before deciding to escape from the mantis than to catch it. Biological significance of these differences in timing was discussed.     

Ultrastructure of Nematode-Trapping Fungi

Capture cells differ ultrastructurally from vegetative cells in the nematode-trapping fungi, Dactylella drechslerii, Monacrosporium rutgeriensis and Arthrobotrys dactyloides, which capture prey by means of adhesive knobs, adhesive hyphal networks, and constricting rings, respectively. Adhesive knobs and adhesive networks contain dense inclusions not found in hyphal tips or subapical regions of the vegetative hyphae, and rough- and smooth-surfaced membranes are abundant in these trap cells. The fine structure of constricting rings differs from that of adhesive traps, and it is altered by closure. In the open configuration, there are membrane-bound inclusions, labyrinthine networks, and electron-lucent regions between the protoplasts and cell wall, all localized on the luminal side of the ring cells. After closure, these features no longer are evident and the cytoplasm of trap cells stains less densely.     

A large improved rotary trap for sampling aerial invertebrates

A large rotary trap designed to measure aerial spider density is described. A comparison of the rotary trap catch and a suction trap catch of spiders and other invertebrate groups showed that the rotary trap was operating at a greater efficiency for most groups. For spiders and non-staphylinid beetles the two traps caught equal numbers. There were no differences in the species composition of spiders between the rotary and suction trap catch.     

The biology of pygmy whitefish, Prosopium coulterii, in a closed sub-boreal lake: spatial distribution and diel movements

Using gillnets and trap nets, we examined the spatial distribution, diel movements, and environmental tolerances of pygmy whitefish, Prosopium coulterii, in a small boreal lake in north-central British Columbia. Most gillnets were set below the thermocline but we also fished a shore net in the littoral zone. During the ice-free season (May to November) there was a strong diel onshore–offshore movement: during the day pygmy whitefish were offshore and below the thermocline (water temperatures of 4–6°C) but at night they were inshore and above the thermocline (water temperatures of 12–18°C). This onshore–offshore movement occurred close to the bottom and, regardless of where they were caught, most fish were <4 m off the bottom. Oxygen concentrations in most of the hypolimnion dropped to <5.0 mg l⁻¹ in June and by late August to <1.0 mg l⁻¹; indicating pygmy whitefish can tolerate low oxygen conditions. The catch of pygmy whitefish in gillnets set below the thermocline was highly skewed: 53% of the nets were empty, 37% caught 18 or less fish, and 10% caught 70% of the total catch (742 fish). Trap nets produced similarly skewed results: most trap net sets caught no pygmy whitefish but one set caught over 2,000 individuals. Our catch data suggest that in Dina Lake #1 some pygmy whitefish aggregate.     

How carnivorous fungi use three-celled constricting rings to trap nematodes

Predacious fungi form specialized hyphae structures to trap nematodes and other microscopic animals. Among the six kinds of trapping devices, the constricting ring is the only one that actively captures nematodes. When a nematode enters the aperture of the ring, which is formed by three cells, the cells rapidly triple their volume, close the aperture and hold the nematode in place. Hyphae then penetrate and consume the nematode. This paper reviews the data and hypotheses on conserving the evolution of constricting rings and their cytological and molecular mechanisms.     

The effects of pitfall trap diameter on ant species richness (Hymenoptera: Formicidae) and species composition of the catch in a semi-arid eucalypt woodland

Abstract Ants play an important role in Australian biodiversity and environmental impact assessments, with pitfall-trapping being the principal sampling method. However, the relationship between trap diameter and ant species catch has not been investigated in the context of survey design. Using four different trap diameters, each at a density of one trap per 100 m2, the present study asks three questions: (i) given an equal number of traps, do traps with larger diameters catch more species than smaller-diameter traps?; (ii) do traps with small diameters bias against large or rare species?; (iii) for equal area of the trap mouth, do small but more numerous traps catch more species than fewer but large traps? A total of 84 species were sampled within the 1600 m2 study site, with numbers of species for trap diameters of: 18mm (46 species), 42mm (56 species), 86mm (62 species) and 135mm (64 species). At equal trap density, 18 mm traps caught significantly fewer species than larger traps. Traps of 86 mm and 135mm were no more efficient than 42mm traps. Only 86mm and 135mm traps caught all species > 10mm in length (6 species). For equal area of the trap mouth, small traps were more efficient than large traps. Differences in the catch of the different-sized traps were due primarily to different capture rates of the rare species (40 species): 18mm traps caught 25% of rare species, 42 mm caught 41%, 86 mm caught 44% and 135 mm caught 52%. The role of rare ant species in environmental impact studies is discussed.     

Rainfall and parasitic wasp (Hymenoptera: Ichneumonoidea) activity in successional forest stages at Barro Colorado Nature Monument, Panama, and La Selva Biological Station, Costa Rica

1 In 1997, we ran two Malaise insect traps in each of four stands of wet forest in Costa Rica (two old‐growth and two 20‐year‐old stands) and four stands of moist forest in Panama (old‐growth, 20, 40 and 120‐year‐old stands). 2 Wet forest traps caught 2.32 times as many ichneumonoids as moist forest traps. The average catch per old‐growth trap was 1.89 times greater than the average catch per second‐growth trap. 3 Parasitoids of lepidopteran larvae were caught in higher proportions in the wet forest, while pupal parasitoids were relatively more active in the moist forest. 4 We hypothesize that moisture availability is of key importance in determining parasitoid activity, community composition and trophic interactions.     

THE COMPARISON OF YELLOW CYLINDRICAL, FLAT AND WATER TRAPS, AND OF JOHNSON SUCTION TRAPS, FOR SAMPLING APHIDS

Different traps were compared to find the type most suitable for studying aphid vectors of plant viruses quantitatively.
A Moericke water trap caught more aphids than a flat sticky trap of equal area. A flat sticky trap (930 sq. cm.) caught half as many aphids as a cylindrical trap (945 sq. cm.), which caught about one-third as many as a water trap (1200 sq. cm.) or a Johnson suction trap (9 in. fan) when operated at between 2 and 3 ft. over bare soil.
Yellow traps caught proportionally more Tuberculoides annulatus , and in summer more Capitophorus species than a suction trap, but significantly fewer Anoecia corni, Sitobium spp. and Pemphigus bursarius. Traps with a level surface caught proportionally more Brevicoryne brassicae, Aphis fabae and Myzus persicae , but fewer Anoecia corni and Drepanosiphum plantanoides than vertical cylindical traps. Attraction by colour influences the catch on horizontal traps more than on cylindrical traps because there is less impaction by the wind.
Only suction traps indicate the number of aphids per unit volume of air and are non-selective, but they are expensive and require an electric power supply. Water traps effectively catch those aphids that are attracted to yellow, but they require frequent attention. Sticky traps catch fewer aphids than either suction or water traps, but they can be left unattended for about 2 weeks. Flat sticky traps catch aphids likely to land on a crop, and cylindrical traps show when aphids are in the air, but not if those aphids are able or wanting to land. For routine work cylindrical sticky traps have other advantages; they are cheap and do not require skilled handling, and their catches of alate Myzus persicae have been correlated with the spread of some plant viruses.     

Field responses of stink bugs to the natural and synthetic pheromone of the Neotropical brown stink bug, Euschistus heros (Heteroptera: Pentatomidae)

Abstract.The synthetic racemic mixture of methyl 2,6,10‐trimethyltridecanoate, a component of the male produced pheromone of Euschistus heros (F.) (Heteroptera, Pentatomidae), was attractive to pentatomid species in a field test, using homemade pheromone trap designs. The pentatomid Piezodorus guildinii was caught in high numbers in field traps, during two field experiments, indicating a consistent response of this species to the E. heros pheromone. A correlation was found between the range of insects caught in the pheromone‐baited traps and a random sampling method. The synthetic stereoisomeric mixture of methyl 2,6,10‐trimethyldodecanoate, a minor component of E. heros pheromone, was also field tested and caught no pentatomids. Egg parasitoids were caught in traps baited with E. heros pheromone, indicating that this pheromone can be exploited as a kairomone. A synchrony in the periodicity of trap catch, between the egg parasitoids and their host, was also recorded.     

Using non-mammalian hosts to study fungal virulence and host defense

Non-mammalian hosts have been used to study host-fungal interactions. Hosts such as Drosophila melanogaster, Caenorhabditis elegans, Acathamoeba castellanii, Dictyostelium discoideum, and Galleria mellonella have provided means to examine the physical barriers, cellular mechanisms and molecular elements of the host response. The Drosophila host-response to fungi is mediated through the Toll pathway, whereas in C. elegans the host-response is TIR-1-dependent. Virulence traits that are involved in mammalian infection are important for the interaction of fungi with these hosts. Screening of fungal virulence traits using mutagenized fungi to determine changes in fungal infectivity of non-mammalian hosts has been used to identify novel virulence proteins used to infect C. elegans such as Kin1 (a serine/threonine protein kinase) and Rom2 (a Rho1 guanyl-nucleotide exchange factor) from Cryptococcus neoformans. These heterologous non-mammalian hosts highlight the similarities and differences between different hosts in fungal pathogenesis and they complement studies in mammalian systems and those using other genetic approaches.     

Development of an odour-baited target for female New World screwworm, Cochliomyia hominivorax: studies with host baits and synthetic wound fluids

Field studies were conducted in Mexico to investigate the possibility of replacing sentinel animals as baits for female New World screwworm Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) with a synthetic odour-bait composed of compounds identified from wounds infested with the larvae of C. hominivorax. Studies using sheep held in stanchions surrounded by an incomplete ring of electrified nets showed that both male and female C. hominivorax were attracted to an unwounded animal (ratio of male : female flies caught 1.11 : 1.00), although they were not observed to land. By contrast, catches associated with unwounded sheep with larval wound fluid applied to shaven shoulders suggested that female C. hominivorax were attracted to (male : female ratio of electrified nets catch 0.14 : 1.00) and landed on the wound fluid but male flies did not respond (male : female ratio alighting on fluid 0.05 : 1.00). In related studies the attractiveness of synthetic blends of 25 electrophysiologically active compounds identified in fluids associated with wounds infested with the larvae of C. hominivorax were tested. The acidic components of the wound fluids were found to attract and elicit a landing response from both male and female flies, whereas the non-acidic components alone caught only low numbers of flies. However, the numbers of male and female flies that were attracted to and landed on a synthetic bait could be increased significantly by increasing the proportion of non-acid to acid components in the lure. In some replicates the most effective blend caught a number of C. hominivorax comparable to that caught by a standard synthetic attractant, Swormlure-4, although, unlike with Swormlure-4, the catch was predominately composed of female flies. It is uncertain whether Swormlure-4 contains compounds that elicit a landing response from male C. hominivorax or whether the synthetic and natural wound odours contain compounds that inhibit a landing response from male C. hominivorax. Further work is required to simplify the blend of compounds needed to attract female flies and to better understand the role of the compounds that elicit behavioural responses from both male and female C. hominivorax before a synthetic substitute can be fully developed to replace sentinel animals.     

Light brown apple moth (Epiphyas postvittana) (Lepidoptera: Tortricidae) colonization of California

In response to the confirmed detection of the light brown apple moth, Epiphyas postvittana, in California, approximately 53,000 pheromone-baited Jackson traps were deployed and more than 246,000 males were caught (February 2007–February 2010). Approximately 46,000 manually entered catch records were corrected for errors and converted into catch per trap per day. As empty trap data (zeros) were not recorded, we added zeros between first and last catch for each trap based on the stated servicing period (~30,000), before analyzing for trends. Residual data error rate was estimated as 1.5 %. San Francisco and Santa Cruz counties had relatively high trap catches immediately upon trap set, and remained the leading population centers, while most other counties showed a more general trend of a slow build-up in catch over time (12 counties). An exponential increase in trap catch was observed in four counties with sufficient data. The pattern of spread indicated natural, as well as anthropogenic-assisted spread rates, with populations appearing well ahead of the invasion front. This jump dispersal is probably due to movement of host plants, unsurprising since eggs of this polyphagous moth are readily laid on foliage. There was evidence of seasonality in spread, probably linked to the phenology of the insect. There was a positive relationship between catch and known host tree preference, suggesting that trap placement in preferred hosts could add sensitivity to future surveys. Recommendations include the improved provision of data acquisition by telecommunications, standardization of data input, more archiving, and frequent analysis of trap catches. The rapid rate of population growth demonstrated in two counties and spread across many others supports the hypothesis of the recent arrival of E. postvittana in California.     

Evaluation of pheromone-baited traps for winter moth and Bruce spanworm (Lepidoptera: Geometridae)

We tested different pheromone-baited traps for surveying winter moth, Operophtera brumata (L.) (Lepidoptera: Geometridae), populations in eastern North America. We compared male catch at Pherocon 1C sticky traps with various large capacity traps and showed that Universal Moth traps with white bottoms caught more winter moths than any other trap type. We ran the experiment on Cape Cod, MA, where we caught only winter moth, and in western Massachusetts, where we caught only Bruce spanworm, Operophtera bruceata (Hulst) (Lepidoptera: Geometridae), a congener of winter moth native to North America that uses the same pheromone compound [(Z,Z,Z)-1,3,6,9-nonadecatetraene] and is difficult to distinguish from adult male winter moths. With Bruce spanworm, the Pherocon 1C sticky traps caught by far the most moths. We tested an isomer of the pheromone [(E,Z,Z)-1,3,6,9-nonadecatetraene] that previous work had suggested would inhibit captures of Bruce spanworm but not winter moths. We found that the different doses and placements of the isomer suppressed captures of both species to a similar degree. We are thus doubtful that we can use the isomer to trap winter moths without also catching Bruce spanworm. Pheromone-baited survey traps will catch both species.