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1.
M. Elizabeth Barnes E. Margaret Evans Ashley Hazel Sara E. Brownell Randolph M. Nesse 《Evolution》2017,10(1):7
Background
How acceptance of evolution relates to understanding of evolution remains controversial despite decades of research. It even remains unclear whether cultural/attitudinal factors or cognitive factors have a greater impact on student ability to learn evolutionary biology. This study examined the influence of cultural/attitudinal factors (religiosity, acceptance of evolution, and parents’ attitudes towards evolution) and cognitive factors (teleological reasoning and prior understanding of natural selection) on students’ learning of natural selection over a semester-long undergraduate course in evolutionary medicine.Method
Pre-post course surveys measured cognitive factors, including teleological reasoning and prior understanding of natural selection, and also cultural/attitudinal factors, including acceptance of evolution, parent attitudes towards evolution, and religiosity. We analyzed how these measures influenced increased understanding of natural selection over the semester.Results
After controlling for other related variables, parent attitude towards evolution and religiosity predicted students’ acceptance of evolution, but did not predict students’ learning gains of natural selection over the semester. Conversely, lower levels of teleological reasoning predicted learning gains in understanding natural selection over the course, but did not predict students’ acceptance of evolution.Conclusions
Acceptance of evolution did not predict students’ ability to learn natural selection over a semester in an evolutionary medicine course. However, teleological reasoning did impact students’ ability to learn natural selection.2.
Background
Simulations can be an active and engaging way for students to learn about natural selection, and many have been developed, including both physical and virtual simulations. In this study we assessed the student experience of, and learning from, two natural selection simulations, one physical and one virtual, in a large enrollment introductory biology lab course. We assigned students to treatments (the physical or virtual simulation activity) by section and assessed their understanding of natural selection using a multiple-choice pre-/post-test and short-answer responses on a post-lab assignment. We assessed student experience of the activities through structured observations and an affective survey.Results
Students in both treatments showed increased understanding of natural selection after completing the simulation activity, but there were no differences between treatments in learning gains on the pre-/post-test, or in the prevalence of concepts and misconceptions in written answers. On a survey of self-reported enjoyment they rated the physical activity significantly higher than the virtual activity. In classroom observations of student behavior, we found significant differences in the distribution of behaviors between treatments, including a higher frequency of off-task behavior during the physical activity.Conclusions
Our results suggest that both simulations are valuable active learning tools to aid students’ understanding of natural selection, so decisions about which simulation to use in a given class, and how to best implement it, can be motivated by contextual factors.3.
N. Cesbron A.-L. Royer Y. Guitton A. Sydor B. Le Bizec G. Dervilly-Pinel 《Metabolomics : Official journal of the Metabolomic Society》2017,13(8):99
Introduction
Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.Objectives
In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting.Methods
The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.Results
A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed.Conclusion
The workflow generated repeatable and informative fingerprints for robust metabolome characterization.4.
Guidetti R Baraldi L Calzolai C Pini L Veronesi P Pederzoli A 《BMC evolutionary biology》2007,7(Z2):S13
Background
Science curricula and teachers should emphasize evolution in a manner commensurate with its importance as a unifying concept in science. The concept of adaptation represents a first step to understand the results of natural selection. We settled an experimental project of alternative didactic to improve knowledge of organism adaptation. Students were involved and stimulated in learning processes by creative activities. To set adaptation in a historic frame, fossil records as evidence of past life and evolution were considered.Results
The experimental project is schematized in nine phases: review of previous knowledge; lesson on fossils; lesson on fantastic animals; planning an imaginary world; creation of an imaginary animal; revision of the imaginary animals; adaptations of real animals; adaptations of fossil animals; and public exposition. A rubric to evaluate the student's performances is reported. The project involved professors and students of the University of Modena and Reggio Emilia and of the "G. Marconi" Secondary School of First Degree (Modena, Italy).Conclusion
The educational objectives of the project are in line with the National Indications of the Italian Ministry of Public Instruction: knowledge of the characteristics of living beings, the meanings of the term "adaptation", the meaning of fossils, the definition of ecosystem, and the particularity of the different biomes. At the end of the project, students will be able to grasp particular adaptations of real organisms and to deduce information about the environment in which the organism evolved. This project allows students to review previous knowledge and to form their personalities.5.
Background
Human populations and breeds of domestic animals are composed of individuals with a multiplicity of eye (= iris) colorations. Some wild birds and mammals may have intraspecific eye color variability, but this variation seems to be due to the developmental stage of the individual, its breeding status, and/or sexual dimorphism. In other words, eye colour tends to be a species-specific trait in wild animals, and the exceptions are species in which individuals of the same age group or gender all develop the same eye colour. Domestic animals, by definition, include bird and mammal species artificially selected by humans in the last few thousand years. Humans themselves may have acquired a diverse palette of eye colors, likewise in recent evolutionary time, in the Mesolithic or in the Upper Paleolithic.Presentation of the hypothesis
We posit two previously unrecognized hypotheses regarding eye color variation: 1) eye coloration in wild animals of every species tends to be a fixed trait. 2) Humans and domestic animal populations, on the contrary, have eyes of multiple colors. Sexual selection has been invoked for eye color variation in humans, but this selection mode does not easily apply in domestic animals, where matings are controlled by the human breeder.Testing the hypothesis
Eye coloration is polygenic in humans. We wish to investigate the genetics of eye color in other animals, as well as the ecological correlates.Implications of the hypothesis
Investigating the origin and function of eye colors will shed light on the reason why some species may have either light-colored irises (e.g., white, yellow or light blue) or dark ones (dark red, brown or black). The causes behind the vast array of eye colors across taxa have never been thoroughly investigated, but it may well be that all Darwinian selection processes are at work: sexual selection in humans, artificial selection for domestic animals, and natural selection (mainly) for wild animals.6.
Monica Calvo-Polanco Wenqing Zhang S. Ellen Macdonald Jorge Señorans Janusz J. Zwiazek 《Plant and Soil》2017,420(1-2):195-208
Aims
Reclamation following oil sands mining in northeastern Alberta (Canada) creates adverse reforestation soil conditions, including extreme pH values. We elucidated pH tolerance limits of boreal plant species and how pH affects nutrient uptake in these plants.Methods
We measured growth, gas exchange, and foliar nutrient concentration of 15 common northern boreal forest plants after eight weeks exposure to root zone pH ranging from 5.0 to 9.0. Cluster analyses were used to group these species based on their pH responses.Results
Based on their growth and gas exchange responses to pH, the 15 plant species could be divided into five groups, each of which contained species that commonly co-occur in particular boreal forest site types. For the foliar nutrient responses to pH, the 15 species could be grouped into only two categories; both showed decreases in foliar N, P, Fe and Zn concentration with increasing pH, with a more pronounced effect on the group that included trembling aspen, paper birch and chokecherry.Conclusions
The evidence of differential adaptation to pH by habitat type suggests the importance of soil pH as a factor affecting boreal plant species distribution and could be helpful for selection of species suitable for reclamation of sites with altered soil pH.7.
Background
Evidence-based practice (EBP) is a complex process. To quantify it, one has to also consider individual and contextual factors using multiple measures. Modern measurement approaches are available to optimize the measurement of complex constructs. This study aimed to develop a robust measurement approach for constructs around EBP including practice, individual (e.g. knowledge, attitudes, confidence, behaviours), and contextual factors (e.g. resources).Methods
One hundred eighty-one items arising from 5 validated EBP measures were subjected to an item analysis. Nominal group technique was used to arrive at a consensus about the content relevance of each item. Baseline questionnaire responses from a longitudinal study of the evolution of EBP in 128 new graduates of Canadian physical and occupational therapy programmes were analysed. Principles of Rasch Measurement Theory were applied to identify challenges with threshold ordering, item and person fit to the Rasch model, unidimensionality, local independence, and differential item functioning (DIF).Results
The nominal group technique identified 70/181 items, and modified Delphi approach identified 68 items that fit a formative model (2 related EBP domains: self-use of EBP (9 items) and EBP activities (7 items)) or a reflective model (4 related EBP domains: attitudes towards EBP (17 items), self-efficacy (9 items), knowledge (11 items) and resources (15 items)). Rasch analysis provided a single score for reflective construct. Among attitudes items, 65% (11/17) fit the Rasch model, item difficulties ranged from ??7.51 to logits (least difficult) to +?5.04 logits (most difficult), and person separation index (PSI)?=?0.63. Among self-efficacy items, 89% (8/9) fit the Rasch model, item difficulties ranged from ??3.70 to +?4.91, and PSI?=?0.80. Among knowledge items, 82% (9/11) fit the Rasch model, item difficulties ranged from ??7.85 to 4.50, and PSI?=?0.81. Among resources items, 87% (13/15) fit the Rasch model, item difficulties ranged from ??3.38 to 2.86, and PSI?=?0.86. DIF occurred in 2 constructs: attitudes (1 by profession and 2 by language) and knowledge (1 by language and 2 by profession) arising from poor wording in the original version leading to poor translation.Conclusions
Rasch Measurement Theory was applied to develop a valid and reliable measure of EBP. Further modifications to the items can be done for subsequent waves of the survey.8.
Christian R. Vogl Brigitte Vogl-Lukasser Michael Walkenhorst 《Journal of ethnobiology and ethnomedicine》2016,12(1):40
Background
The sustainable management of animal health and welfare is of increasing importance to consumers and a key topic in the organic farming movement. Few systematic studies have been undertaken investigating farmers’ local knowledge related to this issue. Ethnoveterinary medicine (EVM) is a discipline focusing on local knowledge and folk methods in veterinary medicine, however most ethnoveterinarian studies primarily address the treatment of animal diseases. Very few studies have explored prophylactic methods.Methods
An ethnoveterinary research project in Eastern Tyrol (Austria) was conducted in 2004 and 2005 to gather information about local knowledge of animal husbandry from 144 informants, with the emphasis on plants that maintain livestock health and welfare.Results
Informants mentioned a total of 87 plants and 22 plant-based generic terms in the context of maintaining and improving livestock health and welfare. The most important preventive measures for maintaining and improving animal health and welfare were practices related to “fodder” and “feeding”. In this category the plants mentioned could be grouped according to three different perceptions about their effect on animals: “Good or bad fodder”, “Functional fodder” and “Fodder medicine”. In addition to fodder, environmental management, the human-animal relationship, household remedies and cultural/religious activities were also mentioned. When asked about practices in the past that maintained animal health and well-being, interviewees mentioned, for example, the importance of the diversity of sources that used to be available to obtain feed and fodder.Conclusions
The informants’ approach that feeding is central to livestock welfare is in line with the standard scientific literature on animal health, including in organic farming. Various scientific studies into common fodder evaluate the nutritive and dietary value, efficiency and safety of fodder. Future studies also have to consider the evaluation of traditional, local fodder resources. In fact, the value of ‘food as medicine’ for humans in the context of local knowledge has been widely assessed, but the potential health benefits of fodder and nutraceuticals in local and traditional ethnoveterinary methods require further attention.9.
Exudate components exert different influences on microbially mediated C losses in simulated rhizosphere soils of a spruce plantation 总被引:1,自引:0,他引:1
Yuanshuang Yuan Wenqiang Zhao Juan Xiao Ziliang Zhang Mingfeng Qiao Qing Liu Huajun Yin 《Plant and Soil》2017,413(1-2):127-144
Aims
An emerging shoot experiences mechanical impedance (MI) prior to initiating photosynthesis, when it needs to break through soil that has a surface crust. This is the one of the first physical stresses that the shoot experiences. Surprisingly, few measurements have been made to understand the impact of this stress upon post-emergent shoot growth.Methods
A system employed wax layers of different strengths to investigate shoot responses to MI of the soil surface. Experiments tested the responses of plants to MI using wax layers with different strengths, and tested different seed sizes, nitrogen and phosphorus nutrition and different wheat genotypes. Detailed leaf and root morphological responses and photosynthetic gas exchange and fluorescence were measured.Results
MI produced permanent impairment to limit plant size, leaf growth rate and leaf photosynthetic function. Large seed sizes and N and P fertilization were able to overcome MI, especially for moderate levels of impedance. There was strong genotypic variation in the response to MI among 14 diverse wheat cultivars, and breeding for varieties suitable to no-tillage cropping systems appears to have facilitated selection in the ability to overcome MI of the soil surface.Conclusions
This study has highlighted the importance of MI stress of the soil surface in limiting shoot growth and has broad implications for plant genotype selection and agricultural systems management, particularly with regard to nutrition and tillage systems.10.
Roman S. Popov Natalia V. Ivanchina Alla A. Kicha Timofey V. Malyarenko Boris B. Grebnev Pavel S. Dmitrenok Valentin A. Stonik 《Metabolomics : Official journal of the Metabolomic Society》2016,12(6):106
Introduction
Starfish are recognized as interesting source of natural steroid products with pharmaceutical potential. Polar steroid metabolites of starfish have unique chemical structures and exhibit various biological activities but their biological functions are controversial.Objectives
The objective of this study was to investigate the response of polar steroid metabolome of the starfish Patiria (=Asterina) pectinifera on various environmental factors and stresses.Methods
Here we first have applied MS-based environmental metabolomics to elucidate the metabolic changes of polar steroid metabolome of starfish. Using HPLC–ESI–Q/TOF–MS approach followed by statistical analysis including principal component analysis and partial least squares discriminant analysis for data classification and potential biomarkers selection, we investigated the changes induced by feeding, injury, variations in water temperature and salinity, and oxygen deficiency.Results
According to multivariate and univariate statistical analysis the responses to feeding, injury and water heating were better expressed than the others and have some similarity in their action on the steroid metabolome of the starfish P. pectinifera. Most constituents of asterosaponin pool were reduced and most constituents of polyhydroxysteroid and related glycoside pool were increased at that.Conclusion
Our results indicate that various metabolic changes in polar steroid constituents of P. pectinifera are induced by feeding and stresses. We believe that these responses are connected with biological multifunctionality of these compounds.11.
Objective
This study examined the differences of psychological strains between Chinese and American college students and discussed how strains may influence individuals’ suicidal ideation and depression.Participants/methods
A total of 539 college students (298 from China and 241 from the U.S.) were recruited in March 2016 to complete the survey study. Multiple linear regressions were used in data analysis.Results
Students in America had higher scores on depression and stress than students in China, while students in China rated higher on suicidal ideation than students in America. In contrast of students in America facing more coping strains, students in China were confronting more value strains in their life.Conclusion
The cross-cultural variations in the effect of psychological strains have been substantiated in current study, indicating that understanding the psychological strains in different cultural contexts is necessary for the early intervention and prevention in college aged populations.12.
Background
Evolution is a difficult subject for students, with well-documented confusion about natural selection, tree thinking, and genetic drift among other topics. Here we investigate the effect of a simulation-based module about the conservation of black-footed ferrets, a module designed with pedagogical approaches that have been demonstrated to be effective, for teaching genetic drift. We compared performance on the Genetic Drift Inventory (GeDI) of students who completed the module and students who were in classes that used other methods for teaching genetic drift.Results
Students in 19 courses using the simulation-based module improved their understanding of genetic drift significantly after completing the Ferrets module, as measured by the GeDI. Students in five control courses actually performed significantly worse on the GeDI after instruction. The lower scores in the control courses were driven by a decrease in these students’ understanding of key concepts.Conclusions
The Ferrets module appears to be an effective way to teach genetic drift. In the control courses, students’ progress in understanding genetic drift may pass through a stage where their understanding of key concepts is worse than it was prior to instruction. However, students who learned genetic drift in courses that used the Ferrets module showed a more rapid increase in their understanding of key concepts related to genetic drift. This result suggests that the paths that students can take to move from novice to expert understanding may be more varied than was previously predicted.13.
Antonella Del-Corso Mario Cappiello Roberta Moschini Francesco Balestri Umberto Mura 《Metabolomics : Official journal of the Metabolomic Society》2018,14(1):2
Introduction
While the evolutionary adaptation of enzymes to their own substrates is a well assessed and rationalized field, how molecules have been originally selected in order to initiate and assemble convenient metabolic pathways is a fascinating, but still debated argument.Objectives
Aim of the present study is to give a rationale for the preferential selection of specific molecules to generate metabolic pathways.Methods
The comparison of structural features of molecules, through an inductive methodological approach, offer a reading key to cautiously propose a determining factor for their metabolic recruitment.Results
Starting with some commonplaces occurring in the structural representation of relevant carbohydrates, such as glucose, fructose and ribose, arguments are presented in associating stable structural determinants of these molecules and their peculiar occurrence in metabolic pathways.Conclusions
Among other possible factors, the reliability of the structural asset of a molecule may be relevant or its selection among structurally and, a priori, functionally similar molecules.14.
Brett L. Bruyere Jonathan Trimarco Saruni Lemungesi 《Journal of ethnobiology and ethnomedicine》2016,12(1):48
Background
The Samburu region of northern Kenya is undergoing significant change, driven by factors including greater value on formal education, improvements in infrastructure and development, a shift from community to private ownership of land, increased sedentary lifestyles and global climate change. One outcome of these changes are an increasingly greater likelihood for adolescent boys to be enrolled in school rather than herding livestock on behalf of the family in a landscape shared with numerous native vegetation and wildlife species.Methods
This study compared identification and knowledge of native plant species between boys enrolled in school with boys of similar age but primary responsibility as herders, called moran. Study participants walked an approximately 100 m path with 10 flagged points in which they were asked to identify any plant species at that point and associated facts of each species, within a 1 m radius.Results
On average, moran identified 38 species compared to 20 for students, including nearly 13 (of a possible 15) species considered to have high cultural significance. Students identified an average of 8.6 culturally-significant plants. Further, moran shared nearly 18 correct facts about the plants, compared with ten for students. In addition, herding frequency was the only significant predictor of plant identification in a linear regression.Conclusion
The results demonstrate that while formal education undoubtedly provides benefits to students, attendance in school in lieu of the traditional role of herders has consequences on young men in Samburu related to ability to identify native and culturally-significant plants. This further shows the importance for communities like those in Samburu undergoing change need to develop alternative options to transmit local traditional knowledge to its younger generations.15.
Saleh Alseekh Luisa Bermudez Luis Alejandro de Haro Alisdair R. Fernie Fernando Carrari 《Metabolomics : Official journal of the Metabolomic Society》2018,14(11):148
Background
Until recently, plant metabolomics have provided a deep understanding on the metabolic regulation in individual plants as experimental units. The application of these techniques to agricultural systems subjected to more complex interactions is a step towards the implementation of translational metabolomics in crop breeding.Aim of Review
We present here a review paper discussing advances in the knowledge reached in the last years derived from the application of metabolomic techniques that evolved from biomarker discovery to improve crop yield and quality.Key Scientific Concepts of Review
Translational metabolomics applied to crop breeding programs.16.
Objective
To examine the validity and reliability of a Japanese version of the Symptom Checklist 90 Revised (SCL-90-R (J)).Methods
The English SCL-90-R was translated to Japanese and the Japanese version confirmed by back-translation. To determine the factor validity and internal consistency of the nine primary subscales, 460 people from the community completed SCL-90-R(J). Test-retest reliability was examined for 104 outpatients and 124 healthy undergraduate students. The convergent-discriminant validity was determined for 80 inpatients who replied to both SCL-90-R(J) and the Minnesota Multiphasic Personality Inventory (MMPI).Results
The correlation coefficients between the nine primary subscales and items were .26 to .78. Cronbach's alpha coefficients were from .76 (Phobic Anxiety) to .86 (Interpersonal Sensitivity). Pearson's correlation coefficients between test-retest scores were from .81 (Psychoticism) to .90 (Somatization) for the outpatients and were from .64 (Phobic Anxiety) to .78 (Paranoid Ideation) for the students. Each of the nine primary subscales correlated well with their corresponding constructs in the MMPI.Conclusion
We confirmed the validity and reliability of SCL-90-R(J) for the measurement of individual distress. The nine primary subscales were consistent with the items of the original English version.17.
Background
The current literature establishes the importance of gene functional category and expression in promoting or suppressing duplicate gene loss after whole genome doubling in plants, a process known as fractionation. Inspired by studies that have reported gene expression to be the dominating factor in preventing duplicate gene loss, we analyzed the relative effect of functional category and expression.Methods
We use multivariate methods to study data sets on gene retention, function and expression in rosids and asterids to estimate effects and assess their interaction.Results
Our results suggest that the effect on duplicate gene retention fractionation by functional category and expression are independent and have no statistical interaction.Conclusion
In plants, functional category is the more dominant factor in explaining duplicate gene loss.18.
Romel Gonzales-Salazar Bianca Cecere Michelina Ruocco Rosa Rao Giandomenico Corrado 《Biotechnology letters》2017,39(7):1049-1058
Objectives
To engineer broad spectrum resistance in potato using different expression strategies.Results
The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B. cinerea. The observed differences between the expression strategies are discussed considering the benefits and features offered by the two systems.Conclusions
Evidence is provided of the possible impact of promoter sequences to engineer BSR in plants, highlighting that the selection of a suitable expression strategy has to balance specific needs and target species.19.
Rafael do Prado Apparecido Eduardo Fermino Carlos Luciano Morais Lião Luiz Gonzaga Esteves Vieira Glaucia Braz Alcantara 《Metabolomics : Official journal of the Metabolomic Society》2017,13(2):20
Introduction
Citrus canker, a disease caused by Xanthomonas axonopodis pv. citri (Xac) bacteria, has been responsible for extensive economic losses in citriculture. In this work, we report the metabolic responses of citrus plants during disease development. This information can be useful for understanding the natural mechanism of plant defense beyond helping design new varieties and/or genetically modified genotypes for tolerance/resistance against citrus canker.Objectives
To understand how primary metabolism is affected in two sweet orange genotypes during citrus canker development.Methods
1H NMR spectroscopy together with chemometrics was used to evaluate the metabolic changes caused by Xac infection at various time points (days 4, 12 and 20) in Citrus sinensis L. Osbeck leaves from non-transgenic and transgenic plants expressing the antibacterial peptide sarcotoxin.Results
The results revealed a high level of metabolic similarity between the studied genotypes without Xac infection. However, after Xac infection, the plants responded differently to disease development. The non-transgenic genotype showed altered early precursors of some secondary metabolites (tryptophan, tyrosine and putrescine) in addition to signaling metabolites of biotic stress (putrescine and dimethylamine), and the drastic reduction of gluconeogenesis was the overall metabolic cost for defense. The transgenic genotype suffered late metabolic changes due to the protective stoichiometric role of sarcotoxin. In addition, the oxidative stress response was more balanced in transgenic than in non-transgenic plants.Conclusion
An NMR-based metabolomic approach was useful for understanding plant–pathogen interactions in citrus canker. Our findings provide valuable preliminary insights into different stages of citrus canker development.20.
Xinchang Kou Tongqing Su Ningning Ma Qi Li Peng Wang Zhengfang Wu Wenju Liang Weixin Cheng 《Plant and Soil》2018,422(1-2):129-134