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1.
Benzimidazoles are anthelmintic agents widely used in the treatment of parasitic infections in a range of species and as fungicidal agents in the control of spoilage of crops during storage and transport. In this paper, the more important benzimidazoles are introduced and their pharmacological effects and physiochemical properties discussed. The metabolism of these drugs is described relating to the occurrence and persistence of residues in biological matrices, providing information for selection of suitable matrices and target residues for testing. Methods for determination of benzimidazoles are reviewed for a range of biological matrices. The importance of selecting suitable extraction and clean-up procedures is discussed, along with the difficulties encountered in adapting single residue methods to multi-residue methods. The importance of suitable detection systems for determination of benzimidazoles, namely, screening, HPLC, GC and confirmatory methods is described in detail. The future for benzimidazole residue analysis is discussed, focusing on selection of appropriate residues for screening methods and protocols for confirmation of benzimidazole residues. 相似文献
2.
MOTIVATION: A number of proteins of known three-dimensional (3D) structure exist, with yet unknown function. In light of the recent progress in structure determination methodology, this number is likely to increase rapidly. A novel method is presented here: 'Rate4Site', which maps the rate of evolution among homologous proteins onto the molecular surface of one of the homologues whose 3D-structure is known. Functionally important regions often correspond to surface patches of slowly evolving residues. RESULTS: Rate4Site estimates the rate of evolution of amino acid sites using the maximum likelihood (ML) principle. The ML estimate of the rates considers the topology and branch lengths of the phylogenetic tree, as well as the underlying stochastic process. To demonstrate its potency, we study the Src SH2 domain. Like previously established methods, Rate4Site detected the SH2 peptide-binding groove. Interestingly, it also detected inter-domain interactions between the SH2 domain and the rest of the Src protein that other methods failed to detect. 相似文献
3.
Antibiotics are widely used in medicine, veterinary medicine, and the food industry. However, the active use of antibacterial drugs leads to environmental pollution. In this regard, there is a great need for monitoring and determining antibiotics in various environments such as drinking water, food, drinks, waste water from pharmaceutical factories, etc. A number of methods, including those based on biosensors, have been developed to determine antibiotics. Biosensor methods of analysis are widely used and are an integral part of environmental monitoring. Electrochemical, optical, acoustic, microbial biosensors, immuno- and aptasensors, as well as sensors based on molecularly imprinted polymers are in the most demand for the analysis of antibiotics. This article provides a brief overview of biosensor methods and approaches for the determination of antibiotics. The most promising biosensor systems for determining antibacterial drugs were analyzed. 相似文献
4.
Modern chromatographic techniques and their application in the determination of toxic compounds in faeces are reviewed. Faecal analysis may be of importance in toxicokinetic studies of xenobiotics in order to determine factors such as metabolism, body burden and major routes of elimination. Compounds of interest include various food constituents, drugs and occupational or environmental factors. Further, various mutagenic or carcinogenic compounds which are excreted by faeces have been indicated to represent risk factors for colorectal cancer. In this context, the chromatographic determination of the endogenously generated fecapentaenes and bile acids, both postulated etiological factors in colorectal carcinogenesis, is reviewed. For fecapentaene determination, several high-performance liquid chromatographic (HPLC) methods are available; however, the applicability of some of these methods is limited owing to insufficient separation of various isomeric forms or discrimination between fecapentaenes and their precursors. For the determination of bile acids in faeces, many chromatographic procedures have been reported, and the characteristics of the most relevant methods are compared and discussed. It is concluded that separation by gas chromatography (GC) in combination with mass spectrometry provides the highest selectivity and sensitivity. A relatively rapid alternative analysis for the determination of total and aqueous faecal bile acids is proposed. Further, methods for the determination of polycyclic aromatic hydrocarbons (PAHs) are reviewed. Although the use of radiolabelled PAHs in animal studies has many advantages, it cannot be applied for human biological monitoring and HPLC and GC provide sensitive alternatives. An HPLC method for the determination of non-metabolized PAHs in faeces is described. 相似文献
5.
The aminoglycosides are a large and diverse class of antibiotics that characteristically contain two or more aminosugars linked by glycosidic bonds to an aminocyclitol component. Structures are presented for over 30 of the most important members of this family of compounds. The use of aminoglycosides in clinical and veterinary medicine and in agriculture is described. Qualitative methods for aminoglycoside analysis include X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). The major part of this article comprises a comprehensive review of quantitative methods for the determination of aminoglycosides. These are microbiological assay, radiochemical assay, radioimmunoassay, enzyme immunoassay, fluoroimmunoassay and other immunoassays, spectrophotometric and other non-separative methods, gas chromatography (GC), thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and capillary electrophoresis (CE). Simple spectrophotometric methods may be adequate for the assay of bulk pharmaceuticals and their formulations. Microbiological assays make useful semi-quantitative screening tests for the analysis of veterinary drug residues in food, but rapid enzyme immunoassays are more suitable for accurate measurements of aminoglycosides in complex matrices. Automated immunoassays are the most appropriate methods for serum aminoglycoside determinations during therapeutic drug monitoring. HPLC techniques provide the specificity and sensitivity required for pharmacokinetic and other research studies, while HPLC–MS is employed for the confirmation of veterinary drug residues. The potential for further development of chromatographic and CE methods for the analysis of biological samples is outlined. 相似文献
6.
In this paper, different electromigration methods used to monitor drugs and polymers released from drug delivery systems are reviewed. First, an introduction to the most typical arrangements used as drug delivery systems (e.g., polymer-drug covalent conjugates, membrane or matrix-based devices) is presented. Next, the principles of different capillary electromigration procedures are discussed, followed by a revision on the different procedures employed to monitor the release of drugs and the degradation or solubilization of the polymeric matrices from drug delivery systems during both in vitro and in vivo assays. A critical comparison between these capillary electrophoretic methods and the more common chromatographic methods employed to analyze drugs and polymers from drug delivery systems is presented. Finally, future outlooks of these electromigration procedures in the controlled release field are discussed. 相似文献
7.
Five methodologies for extracting DNA from food samples are described. The food products analyzed are from either soybean or maize. They were selected on the basis of the mechanical, thermal, and chemical treatments that they had been subjected to during industrial processing. DNA preparations were evaluated for purity, yield, and average fragment size. Two endogenous genes, soybean lectin gene and alcohol dehydrogenase gene (adh1), were used to assess the degree of DNA degradation at different stages of the transformation chain. The goal of this study was to determine the role that extraction methods play in DNA amplification in order to select the best protocol for a food sample. This comparative evaluation can be specifically useful for detection of genetically modified ingredients in a variety of food matrices. 相似文献
8.
There has been considerable interest in the problem of making maximum likelihood (ML) evolutionary trees which allow insertions and deletions. This problem is partly one of formulation: how does one define a probabilistic model for such trees which treats insertion and deletion in a biologically plausible manner? A possible answer to this question is proposed here by extending the concept of a hidden Markov model (HMM) to evolutionary trees. The model, called a tree-HMM, allows what may be loosely regarded as learnable affine-type gap penalties for alignments. These penalties are expressed in HMMs as probabilities of transitions between states. In the tree-HMM, this idea is given an evolutionary embodiment by defining trees of transitions. Just as the probability of a tree composed of ungapped sequences is computed, by Felsenstein's method, using matrices representing the probabilities of substitutions of residues along the edges of the tree, so the probabilities in a tree-HMM are computed by substitution matrices for both residues and transitions. How to define these matrices by a ML procedure using an algorithm that learns from a database of protein sequences is shown here. Given these matrices, one can define a tree-HMM likelihood for a set of sequences, assuming a particular tree topology and an alignment of the sequences to the model. If one could efficiently find the alignment which maximizes (or comes close to maximizing) this likelihood, then one could search for the optimal tree topology for the sequences. An alignment algorithm is defined here which, given a particular tree topology, is guaranteed to increase the likelihood of the model. Unfortunately, it fails to find global optima for realistic sequence sets. Thus further research is needed to turn the tree-HMM into a practical phylogenetic tool. 相似文献
9.
Genetic correlations are frequently estimated from natural and experimental populations, yet many of the statistical properties of estimators
of are not known, and accurate methods have not been described for estimating the precision of estimates of Our objective was to assess the statistical properties of multivariate analysis of variance (MANOVA), restricted maximum
likelihood (REML), and maximum likelihood (ML) estimators of by simulating bivariate normal samples for the one-way balanced linear model. We estimated probabilities of non-positive
definite MANOVA estimates of genetic variance-covariance matrices and biases and variances of MANOVA, REML, and ML estimators
of and assessed the accuracy of parametric, jackknife, and bootstrap variance and confidence interval estimators for MANOVA estimates of were normally distributed. REML and ML estimates were normally distributed for but skewed for and 0.9. All of the estimators were biased. The MANOVA estimator was less biased than REML and ML estimators when heritability
( H), the number of genotypes ( n), and the number of replications ( r) were low. The biases were otherwise nearly equal for different estimators and could not be reduced by jackknifing or bootstrapping.
The variance of the MANOVA estimator was greater than the variance of the REML or ML estimator for most H, n, and r. Bootstrapping produced estimates of the variance of close to the known variance, especially for REML and ML. The observed coverages of the REML and ML bootstrap interval estimators
were consistently close to stated coverages, whereas the observed coverage of the MANOVA bootstrap interval estimator was
unsatisfactory for some H,
n, and r. The other interval estimators produced unsatisfactory coverages. REML and ML bootstrap interval estimates were narrower
than MANOVA bootstrap interval estimates for most H, and r.
Received: 6 July 1995 / Accepted: 8 March 1996 相似文献
10.
Mycotoxins are natural contaminants produced by a range of fungal species. Their common occurrence in food and feed poses
a threat to the health of humans and animals. This threat is caused either by the direct contamination of agricultural commodities
or by a “carry-over” of mycotoxins and their metabolites into animal tissues, milk, and eggs after feeding of contaminated
hay or corn. As a consequence of their diverse chemical structures and varying physical properties, mycotoxins exhibit a wide
range of biological effects. Individual mycotoxins can be genotoxic, mutagenic, carcinogenic, teratogenic, and oestrogenic.
To protect consumer health and to reduce economic losses, surveillance and control of mycotoxins in food and feed has become
a major objective for producers, regulatory authorities and researchers worldwide. However, the variety of chemical structures
makes it impossible to use one single technique for mycotoxin analysis. Hence, a vast number of analytical methods has been
developed and validated. The heterogeneity of food matrices combined with the demand for a fast, simultaneous and accurate
determination of multiple mycotoxins creates enormous challenges for routine analysis. The most crucial issues will be discussed
in this review. These are (1) the collection of representative samples, (2) the performance of classical and emerging analytical
methods based on chromatographic or immunochemical techniques, (3) the validation of official methods for enforcement, and
(4) the limitations and future prospects of the current methods. 相似文献
11.
The application of capillary electrophoresis (CE) methods in forensic toxicology for the determination of illicit and/or misused drugs in biological samples is reviewed in the present paper. Sample pretreatments and direct injection modes used in CE for analysis of drugs in biological fluids are briefly described. Besides, applications of separation methods based on capillary zone electrophoresis or micellar electrokinetic chromatography with UV absorbance detection to (i) analysis of drugs of abuse, (ii) analysis of other drugs and toxicants of potential forensic interest and (iii) for metabolism studies are reviewed. Also, alternative CE methods are briefly discussed, including capillary isotachophoresis and separation on mixed polymer networks. High sensitivity detection methods used for forensic drug analysis in biological samples are then presented, particularly those based on laser induced fluorescence. A glimpse of the first examples of application of CE–mass spectrometry in forensic toxicology is finally given. 相似文献
12.
Neutron activation analysis methods for determination of rare-earth elements in different matrices have been developed at the University of Pavia using the 250 Kw TRIGA Mark II reactor. A critical review of both instrumental and destructive methods is presented, as well as the indication of the best working conditions for irradiation, counting and radiochemical separations. The optimized procedures were utilized in the determination of rare-earth elements in standard reference materials of both mineral and biological origin. The adopted radiochemical procedure is based on the separation of the rare-earth element group by fluoride precipitation.Results, given as the average of six independent determinations and relative standard deviations, are reported and discussed. Precision of the methods can be deduced from the reproducibility of data, whereas accuracy is evaluated by comparison with existing values in the literature. Sensitivity limits under the described operational conditions are also reported, as are trends and correlations among data. 相似文献
13.
Most available antiparasitic drugs are safe, cheap and highly effective against a broad spectrum of parasites. However, the alarming increase in the number of parasite species that are resistant to these drugs, the issue of residues in the food chain and the lack of new drugs stimulate development of alternative control methods in which vaccines would have a central role. Parasite vaccines are still rare, but there are encouraging signs that their number will increase in the next decade. The modern paradigm is that an understanding of parasite genes will lead to the identification of useful antigens, which can then be produced in recombinant systems developed as a result of the huge investment in biotechnology. However, we should also continue to devote efforts to basic research on the host-parasite interface. 相似文献
14.
A series of 21 multiplex (MP) polymerase chain reactions containing simple sequence repeat (SSR) markers spanning most of
the apple genome has been developed. Eighty-eight SSR markers, well distributed over all 17 linkage groups (LGs), have been
selected. Eighty-four of them were included in 21 different MPs while four could not be included in any MPs. The 21 MPs were
then used to genotype approximately 2,000 DNA samples from the European High-quality Disease-Resistant Apples for a Sustainable
agriculture project. Two SSRs (CH01d03 and NZAL08) were discarded at an early stage as they did not produce stable amplifications
in the MPs, while the scoring of the multilocus (ML) SSR Hi07d11 and CN44794 was too complex for large-scale genotyping. The
testing of the remaining 80 SSRs over a large number of different genotypes allowed: (1) a better estimation of their level
of polymorphism; as well as of (2) the size range of the alleles amplified; (3) the identification of additional unmapped
loci of some ML SSRs; (4) the development of methods to assign alleles to the different loci of ML SSRs and (5) conditions
at which an SSR previously described as ML would amplify alleles of a single locus to be determined. These data resulted in
the selection of 75 SSRs out of the 80 that are well suited and recommended for large genotyping projects.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Selenium belongs to a group of trace elements of special interest in biological samples for clinical diagnosis. Selenium has antioxidizing functions and is essential for providing the organism with triiodothyronine produced from thyroxine. Among several analytical techniques used to determine the Se concentration in serum, Inductively Coupled Plasma Mass Spectrometry (ICP-MS) has been used in the past because of its high sensitivity. Interference problems originating from different ions on the major Se isotopes have been described to be a limiting factor for the direct determination of Se in these matrices. Standard addition calibration or isotope dilution is often required to overcome carbon-enhanced ionisation effects in biological sample matrices. In most cases, the typical serum sample volume which is available for the analysis is limited to 0.5 ml or less, making multiple sample preparation for standard addition calibration impractical. Isotope dilution requires enriched isotopes and substantial sample preparation. Furthermore, the approximate Se concentration in every sample has to be known to adjust the appropriate amount of spike to each sample. Matrix matching with methanol has been described to overcome ionisation effects but we found limiting factors of this application when other trace elements are also determined within one sample run. This paper describes an effective sample preparation method which allows the direct determination of Se in serum without limiting the analytical capabilities for the additional determination of Al, Cu, Ni, Co, Cd, Mn and Zn in a single sample run by ICP-MS. Optimization procedures are presented and results of the analysis of reference samples are discussed, with a comparison of more than 150 serum data with those obtained by the GF-AAS method. 相似文献
16.
Analytical chemistry aims at developing analytical methods and techniques for unequivocal identification and accurate quantitation of natural and synthetic compounds in a given matrix. Analytical methods based on the mass spectrometry (MS) technology, e.g., GC/MS and LC/MS and their variants, GC/tandem MS and LC/tandem MS, are best suited both for qualitative and quantitative analyses. GC/MS methods not only serve as reference methods, e.g., in clinical chemistry, but they are now widely and routinely used for quantitative determination of numerous analytes. However, despite inherent accuracy, analytical methods based on GC/MS commonly consist of several analytical steps, including extraction and derivatization of the analyte. In general, unequivocal identification and accurate quantification of an analyte in very low concentrations in complex matrices require further chromatographic techniques, such as high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for sample purification. In recent years, affinity chromatography (e.g., boronate and immunoaffinity chromatography) has been developed to a superior technique for sample preparation of numerous classes of compounds in GC/MS. In this article, the application and importance of affinity chromatography as a method for sample preparation in modern quantitative GC/MS method is described and discussed, using as examples various natural and synthetic compounds, such as arachidonic acid derivates, nitrosylated and nitrated proteins, steroids, drugs, and toxins. 相似文献
17.
卡那霉素是一种氨基糖苷类抗生素,由于其效果好、价格低等优点,是我国常用兽药之一。但如果剂量过大就会大量残留在动物体内,进而通过食物链富集进入人体,引发耳毒性、肾毒性等毒副作用,严重时会导致人死亡,因此对其含量的检测十分重要。近几年,大量基于核酸适配体检测卡那霉素的光学方法被开发,以满足人们对其检测的需求。首先明确了核酸适配体与光学生物传感器等相关概念;再根据其反应机制不同,对光学方法介导的生物传感器进行了分类综述,阐述了各类传感器的基本原理及其检测范围;最后对这几类传感器的优缺点和发展前景进行了总结和展望,对这些方法的进一步探索将为食品安全检测提供新的技术支撑。 相似文献
18.
Alkylphosphocholines represent a new class of drugs with remarkable antineoplastic and antiprotozoal activity. For instance, hexadecylphosphocholine has been approved for the topical treatment of skin metastasis. In addition, it was successfully studied in India for the treatment of leishmaniasis. Different phase-I and phase-II-trials resulted in cure rates of more than 97%. To optimize antitumor or antiprotozoal activity, we have prepared alkylphosphocholines differing in chain length and unsaturation. For the qualitative and quantitative analysis of these longer chain analogues, we have used isocratic high performance liquid chromatography. The separation of the alkylphosphocholines with different chain lengths in this reversed phase HPLC system was achieved on a YMC-TMS column with a mobile phase consisting of methanol-water (85:15; v/v) at a flow rate of 1.0 ml/min. Furthermore the cis-/trans-isomers such as oleylphosphocholine and elaidylphosphocholine were clearly separated on a YMC-C8 column with a methanol-water mixture (80:20; v/v) as mobile phase. In the described reversed phase HPLC systems simple refractive index detection and UV detection allow the sensitive and quantitative determination of alkylphosphocholines. These methods are very important for reproducible identification and quantitative determination of saturated and mono-unsaturated alkylphosphocholines with alkyl residues containing up to 25 carbon atoms. 相似文献
19.
The analysis of solvent residues trapped into crystals of illicit drugs provides useful evidence for monitoring current use trend in the chemical underground, and is also a suitable tool to achieve the complete chemical characterisation of street drugs for comparative examination of separate specimens. This paper describes a method developed in order to perform simultaneous qualitative and quantitative analysis of solvent residues in cocaine samples. The method is based on GC-MS analysis of solvents after their extraction/concentration from drug matrices accomplished by solid-phase microextraction (SPME) in static head space. The proposed method has been used to detect residues of solvents in 47 illicit street cocaine samples. Quantitative analyses were carried out only for the solvents identified at concentration values higher than 1 ppm. Statistical evaluation of our results allowed us to group the illicit samples into various classes according to different kinds of residual solvent, in connection with different clandestine manufacturing processes used to prepare illicit cocaine. 相似文献
20.
Vancomycin is one of a family of related macrocyclic glycopeptide antibiotics that were discovered by scientists at the Eli Lilly Company in the 1950s. It has been used to treat severe staphylococcal infections, particularly when bacterial resistance to other antibiotics has developed. Vancomycin is a naturally occurring chiral compound and has a number of stereogenic centers. Furthermore, it contains a variety of functionalities that are known to be useful for enantioselective interactions (e.g., hydrogen bonding groups, hydrophobic pockets, aromatic groups, amide linkages, etc.). The physicochemical properties of vancomycin, including its stability in solution, are discussed as they pertain to capillary electrophoresis. Over 100 racemates were resolved including many nonsteroidal antiinflammatory drugs, antineoplastic compounds and N-derivatized amino acids. Many of these compounds had very high resolution factors. Optimization and the effect of different experimental parameters on the enantioselective separations are discussed. © 1994 Wiley-Liss, Inc. 相似文献
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