湖北宜昌黄花场早奥陶世疑源类的新类型

该文报道了湖北宜昌黄花场下奥陶统大湾组下段的疑源类（海洋微体浮游植物化石）新类型,描述和建立３新属：Ｐａｐｉｌｌｉｆｅｒｕｍｇｅｎ．ｎｏｖ．,１ｇｅｎ．ｎｏｖ．和Ｙｉｃｈａｎｇｉａｇｅｎ．ｎｏｖ．;２新种：Ｐａｐｉｌｌｉｆｅｒｕｍｉｒｒｅｇｕｌａｒｅｇｅｎ。ｅｔｓｐ．ｎｏｖ．,Ｙｉｃｈａｎｇｉａｂｉｐｏｒｏｓａｇｅｎ;ｅｔｓｐ。ｎｏｖ。及重新修改和组合的３属、种：Ｐａｐｉｌｌｉｆｅｒｕｍｒｈａｂｄｏｃｌａｄｉｕｍ（Ｌｕ）ｃｏｍｂ．ｅｔｅｍｅｎｄ．ｎｏｖ．,Ｐ。ｓｔｒｉａｔｕｍ（Ｌｕ）ｃｏｍｂ．ｅｔｅｍｅｎｄ。ｎｏｖ。和Ｓｔｅｌｏｍｏｒｐｈａｅｒｃｈｕｎｅｎｓｉｓ（Ｆａｎｇ）ｃｏｍｂ;ｅｔｅｍｅｎｄ．ｎｏｖ．这些疑源类新类型目前仅见于我国西南的部分地区的早奥陶世地层。它们为探讨早奥陶世海洋微体浮游植物的区域分布及扬子地台的古地理位置,提供了重要的证据．     

Isolation and morphology of helically sculptured,rosette-forming,freshwater bacteria resemblingSeliberia

Nine independently isolated bacterial strians of helically sculptured rods were obtained from dilute peptone enrichments of freshwater sources. Enrichment and isolation procedures exploited the oligotrophic character of these bacteria. They exhibited tenacious adhesiveness, asymmetric fission or budding (which was dependent on the cultural conditions), and a polar or subpolar flagellum in their motile stage. Their adhesiveness was mediated by an excreted holdfast at one pole; stellate aggregation (rosette formation) was commonly observed. Motile daughter cells were generated from the apical end of the rod, opposite the adhesive or attached pole. Their fine pili (4 nm diameter) did not seem to be involved in aggregation. Growth on media containing ulmic acid stimulated production of spherical or oval cell-like bodies. These aquatic isolates resemble the soil bacteriumSeliberia stellata in many of their morphological features.     

Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins

Type IV pili (T4P) are surface structures that undergo extension/retraction oscillations to generate cell motility. In Myxococcus xanthus , T4P are unipolarly localized and undergo pole-to-pole oscillations synchronously with cellular reversals. We investigated the mechanisms underlying these oscillations. We show that several T4P proteins localize symmetrically in clusters at both cell poles between reversals, and these clusters remain stationary during reversals. Conversely, the PilB and PilT motor ATPases that energize extension and retraction, respectively, localize to opposite poles with PilB predominantly at the piliated and PilT predominantly at the non-piliated pole, and these proteins oscillate between the poles during reversals. Therefore, T4P pole-to-pole oscillations involve the disassembly of T4P machinery at one pole and reassembly of this machinery at the opposite pole. Fluorescence recovery after photobleaching experiments showed rapid turnover of YFP–PilT in the polar clusters between reversals. Moreover, PilT displays bursts of accumulation at the piliated pole between reversals. These observations suggest that the spatial separation of PilB and PilT in combination with the noisy PilT accumulation at the piliated pole allow the temporal separation of extension and retraction. This is the first demonstration that the function of a molecular machine depends on disassembly and reassembly of its individual parts.     

Differences in Fine Structures between Normal and RNA-induced Drosophila Pole Cells

Fine structures were compared between normal pole cells and those induced in embryos that had been uv-irradiated and then injected with intact polar plasm or with poly(A)⁺RNA extracted from cleavage embryos. Nuclei in nomal pole cells were spherical. In contrast, those in the induced pole cells were deformed to variable extents depending on materials injected with. Polar granules were smaller in pole cells induced by injection of poly(A)⁺RNA than in normal pole cells. The size of polar granules in polar-plasm-induced pole cells was intermediate between those in poly(A)⁺RNA-induced and normal pole cells. Small polar granules were observed in posterior cells of embryos uv-irradiated, nevertheless those cells were columnar and with identical morphology to somatic cells. Nuclear bodies showed a similar tendency in size differences as observed in polar granules in three types of pole cells observed.     

The ultrastructure and reproduction of Amphiamblys capitellides (Microspora, Metchnikovellidae), a parasite of the gregarine Ancora sagittata (Apicomplexa, Lecudinidae), with redescription of the species and comments on the taxonomy

The ultrastructural cytology and reproduction of the hyperparasitic microsporidium Amphiamblys capitellides (Caullery and Mesnil, 1897) is described. Merogonial reproduction was not observed. The sporogony comprises two sequences: a sac-bound sporogony in close contact with the cytoplasm of the host and a free sporogony in parasitophorous vacuoles. The free sporogony, which probably precedes the sac-bound, yields a small number of rounded spores. The sac-bound sporogony is polysporoblastic, generating two rows of elongated spores. All stages have isolated nuclei. Both spore types have an extrusion apparatus of the metchnikovellidean type, with a polar sac devoid of anchoring disc, a polar filament with one manubroid and one bulbous part, and a posterior semicircular membrane fold enclosing rounded or tubular structures. Hosts are gregarines of the species Ancora sagittata living in the intestine of polychaetes of the genus Capitella, probably the species Capitella giardi. The cytology, life cycle and classification are discussed. The species is redescribed and the diagnosis of the genus Amphiamblys Caullery and Mesnil, 1914 is emended.     

On some species of microfossils from the Torgin formation of the Neoproterozoic of the Berezov Depression (Southern Siberian Platform)

A new genus, Soktokuta (Rhodophyta), and new species S. sporifera, Talakania diversa, Torgia munientis, and T. ellipsoidea are described. The diagnosis of the genus Torgia Grigorieva et Kolosov is emended. The genus Torgia is classified within the Chlorophyta. The Late Riphean (Neoproterozoic) beds of the South Urals and Siberia contain ellipsoidal unicellular fronds (Chlorophyta) with polar structures (dense and frequently spiny terminations).     

Ultrastructure of cell division in the marine red algaLomentaria baileyana

Summary Mitosis in the marine red algaLomentaria baileyana (Rhodymeniales, Rhodophyta) was studied with the electron microscope. Nucleus associated organelles known as polar rings (PRs) migrate to establish the division poles at prophase. At prometaphase, shallow invaginations in the nuclear envelope (NE) form on two sides of each PR and soon rupture. The gaps that are consequently formed contain several small fragments of NE. A larger region of NE remains intact between the two gaps. By metaphase several cisternae of perinuclear endoplasmic reticulum (PER) have enclosed most of the nucleus but remain absent from the polar regions. The nucleolus disperses partially and a typical metaphase plate of chromosomes is formed. Each PR has disjoined into separate proximal and distal portions. MTs converge widely on all regions of the polar area, but do not extend into the cytoplasm. Some MTs end near or at the chromosomes while others extend slightly farther past the chromosomes or diagonally to the NE. As chromosomes move to opposite poles at anaphase, they are accompanied by nucleolar material. An interzonal midpiece (IZM) is created as the pole to pole distance increases and the NE remains intact except for the polar gaps. Following detachment from the IZM, the daughter nuclei are separated by a large central vacuole as a cleavage furrow develops and eventually constricts to form two cells following pit connection formation. It is suggested that mitosis inLomentaria represents an evolutionary intermediate between that seen in the higher and lower groups of red algae. This conclusion is in agreement with conventional morphological and light microscopic criteria used to placeLomentaria in theRhodymeniales, which is considered to be the next to most advanced order in theRhodophyta.     

Infraciliature and morphogenesis in three rumen Diplodinium ciliates, Diplodinium polygonale, Diplodinium leche, and Diplodinium nanum, observed by light microscopy

Infraciliature and morphogenesis of three rumen ophryoscolecid ciliates, Diplodinium polygonale Dogiel, 1925, Diplodinium leche Imai et al., 1992, and Diplodinium nanum Imai, 1988, are described from pyridinated silver carbonate-impregnated specimens. These three species have two polybrachykineties in the buccal area and a polybrachykinety in the dorsal ciliary zone. The vestibular polybrachykinety (VP) of D. polygonale and D. leche arises from the dorsal extremity of the adoral polybrachykinety (AP) as in Entodinium species, extending toward the left in D. polygonale and toward the left posterior in D. leche. The VP of D. nanum arises from the inner side of the AP, separate from its dorsal extremity, as in other Diplodinium species and extends toward the left posterior. These series of the polybrachykinety arrangements in D. polygonale, D. leche, and D. nanum can be regarded as transitional forms in the evolution of an Entodinium-like ancestor to Diplodinium. Morphogenesis of these three Diplodinium species is not different from that of other Diplodinium species.     

Beschorneria yuccoides and Asimina triloba (L.) Dun: Examples for proximal polar germinating pollen in angiosperms

Beschorneria yuccoides (Agavaceae) microspores are arranged mostly in planar tetrads. Later on, the pollen grains of the tetrad usually fall apart, but sometimes remain loosely connected by ektexine elements. The ektexine consists of a tectum, of short columellae, and of a thin, discontinuous foot layer. An endexine is absent. The bilayered intine is without any additional thickening that would usually indicate an aperture region. From this point of view the pollen grain might be considered as omniaperturate. The pollen ornamentation is reticulate with wide lumina and robust, smooth muri.

The pollen grains show an indistinct sulcus characterised by a loose reticulate ornamentation. The sulcus is not exactly at the distal pole, but shifted towards the equator. No pollen tubes are formed regularly at the sulcus. Instead, pollen tubes are normally formed at the proximal pollen face. The proximal area, indicating a large germination field, is morphologically and functionally clearly an aperture (a germination zone); however, it does not represent a sulcus. The proximal face of all pollen grains appears as ornamented, with some exine lumps.

Asimina triloba (Annonaceae) pollen is shed in permanent planar or decussate tetrads. The distal sides are microreticulate to foveolate, and do not show an aperture; the psilate proximal sides are the germination areas of A. triloba.

The presence of apertures placed at the proximal pole was reported for distinct taxa of several angiosperm families. For Drosera, Dionaea (Droseraceae) and most probably for the diaperturate Cuphea species (Lythraceae) the existence of polar germination areas can be excluded. However, in some Annonaceae taxa with permanent tetrads (Annona cherimola, Asimina triloba) a situation similar to Beschorneria might be present, and indeed a proximal polar pollen tube is formed. Beschorneria yuccoides, Annona cherimola and Asimina triloba are unequivocal examples of angiosperm pollen with an exactly proximal aperture (germination area).     

Structural evidence for a dominant role of nonpolar interactions in the binding of a transport/chemosensory receptor to its highly polar ligands.

The receptor, a maltose/maltooligosaccharide-binding protein, has been found to be an excellent system for the study of molecular recognition because its polar and nonpolar binding functions are segregated into two globular domains. The X-ray structures of the "closed" and "open" forms of the protein complexed with maltose and maltotetraitol have been determined. These sugars have approximately 3 times more accessible polar surface (from OH groups) than nonpolar surface (from small clusters of sugar ring CH bonds). In the closed structures, the oligosaccharides are buried in the groove between the two domains of the protein and bound by extensive hydrogen bonding interactions of the OH groups with the polar residues confined mostly in one domain and by nonpolar interactions of the CH clusters with four aromatic residues lodged in the other domain. Substantial contacts between the sugar hydroxyls and aromatic residues are also formed. In the open structures, the oligosaccharides are bound almost exclusively in the domain rich in aromatic residues. This finding, along with the analysis of buried surface area due to complex formations in the open and closed structures, supports a major role for nonpolar interactions in initial ligand binding even when the ligands have significantly greater potential for highly specific polar interactions.     

Drosophila tudor is essential for polar granule assembly and pole cell specification, but not for posterior patterning

Pole cells and posterior segmentation in Drosophila are specified by maternally encoded genes whose products accumulate at the posterior pole of the oocyte. Among these genes is tudor (tud). Progeny of hypomorphic tud mothers lack pole cells and have variable posterior patterning defects. We have isolated a null allele to further investigate tud function. While no pole cells are ever observed in embryos from tud-null mothers, 15% of these embryos have normal posterior patterning. OSKAR (OSK) and VASA (VAS) proteins, and nanos (nos) RNA, all initially localize to the pole plasm of tud-null oocytes and embryos from tud-null mothers, while localization of germ cell-less (gcl) and polar granule component (pgc), is undetectable or severely reduced. In embryos from tud-null mothers, polar granules are greatly reduced in number, size, and electron density. Thus, tud is dispensable for somatic patterning, but essential for pole cell specification and polar granule formation.     

Near-neighbor analysis of spindle microtubules in the alga Ochromonas

The near-neighbor spacing of microtubules (MTs) in the spindle of the alga Ochromonas is analyzed. The technique of near-neighbor analysis of MTs (as developed by McDonald et al. [9]) in the mid-region of the Ochromonas spindle (overlap) shows that MTs from one pole preferentially associate with MTs from the opposite pole at a center-to-center distance of 35 to 43 nm. However, in the half spindle between the chromosomes and the poles, kinetochore MTs (kMTs) do not preferentially associate with other MTs in the half spindle but instead are arranged essentially at random. Individual polar MTs (MTs attached to one pole), kMTs and free MTs (MTs unattached to the poles) were selected for near-neighbor analysis over their entire lengths. The spacing of MTs in the overlap is compatible with those models for mitosis which propose that separation of the poles is accomplished by sliding between closely spaced MTs of opposite polarity. In contrast to the overlap, the arrangement of MTs in the half spindle is not compatible with MT2MT sliding theories that propose that chromosome movement is accomplished by sliding between kMTs and polar MTs.     

On morphology and taxonomy of Ammoastuta Cushman and Brönnimann 1948 (protista: foraminiferida)

The morphology of the brackish waterAmmoastuta salsa Cushman and Brönnimann, the type species ofAmmoastuta, is revised and the genus definition emended. In view of the early planispiral coil, the late uniserial development which tends toward rectilinear, the imperforate agglutinated wall, the multiple aperture and the simple interior, the Ammoastutinae Loeblich and Tappan 1984, are placed into the Lituolidae de Blainville 1827, emended herein, of the Lituolacea DE Blainville 1827, emended herein.     

SpbR overproduction reveals the importance of proteolytic degradation for cell pole development and chromosome segregation in Caulobacter crescentus

In most rod‐shaped bacteria, DNA replication is quickly followed by chromosome segregation, when one of the newly duplicated centromeres moves across the cell to the opposite (or ‘new’) pole. Two proteins in Caulobacter crescentus, PopZ and TipN, provide directional cues at the new pole that guide the translocating chromosome to its destination. We show that centromere translocation can be inhibited by an evolutionarily conserved pole‐localized protein that we have named SpbR. When overproduced, SpbR exhibits aberrant accumulation at the old pole, where it physically interacts with PopZ. This prevents the relocation of PopZ to the new pole, thereby eliminating a positional cue for centromere translocation. Consistent with this, the centromere translocation phenotype of SpbR overproducing cells is strongly enhanced in a ?tipN mutant background. We find that pole‐localized SpbR is normally cleared by ClpXP‐mediated proteolysis before the time of chromosome segregation, indicating that SpbR turnover is part of the cell cycle‐dependent program of polar development. This work demonstrates the importance of proteolysis as a housekeeping activity that removes outgoing factors from the developing cell pole, and provides an example of a substrate that can inhibit polar functions if it is insufficiently cleared.     

Polarized cells, polar actions.

The recognition of polar bacterial organization is just emerging. The examples of polar localization given here are from a variety of bacterial species and concern a disparate array of cellular functions. A number of well-characterized instances of polar localization of bacterial proteins, including the chemoreceptor complex in both C. crescentus and E. coli, the maltose-binding protein in E. coli, the B. japonicum surface attachment proteins, and the actin tail of L. monocytogenes within a mammalian cell, involve proteins or protein complexes that facilitate bacterial interaction with the environment, either the extracellular milieux or that within a plant or mammalian host. The significance of this observation remains unclear. Polarity in bacteria poses many problems, including the necessity for a mechanism for asymmetrically distributing proteins as well as a mechanism by which polar localization is maintained. Large structures, such as a flagellum, are anchored at the pole by means of the basal body that traverses the peptidoglycan wall. But for proteins and small complexes, whether in the periplasm or the membrane, one must invoke a mechanism that prevents the diffusion of these proteins away from the cell pole. Perhaps the periplasmic proteins are retained at the pole by the presence of the periseptal annulus (35). The constraining features for membrane components are not known. For large aggregates, such as the clusters of MCP, CheA, and CheW complexes, perhaps the size of the aggregate alone prevents displacement. In most cases of cellular asymmetry, bacteria are able to discriminate between the new pole and the old pole and to utilize this information for localization specificity. The maturation of new pole to old pole appears to be a common theme as well. Given numerous examples reported thus far, we propose that bacterial polarity displays specific rules and is a more general phenomenon than has been previously recognized.     

Isolation of polar granules and the identification of polar granule-specific protein.

During early embryogenesis in Drosophila melanogaster the posterior polar plasm has the capacity to induce the formation of primordial germ cells. Polar granules, organelles located exclusively in this polar plasm, have been implicated in this determinative capacity. Using cell populations enriched for pole cells as starting material, we have obtained a particulate subcellular fraction that by EM analysis consists predominately of polar granules. The chemical nature of the polar granule has been defined by establishing a strict correlation between the morphological entity and the presence of specific chemical components. We have identified a basic protein with a molecular weight on SDS-polyacrylamide gels of 95,000 daltons that is unique to embryonic cell populations containing pole cells. This protein is enriched specifically in particulate subcellular fractions containing polar granules and is the only major protein species present in preparations in which polar granules are the major morphological constituent. Based on these data, polar granules appear to be composed primarily of one major basic protein species.     

A myxobacterial S-motility protein dances with poles

Coordinated movement of packs of S-motile Myxococcus xanthus cells relies on extrusion and retraction of pili that are located at one cell pole. At regular intervals the pili switch their polar location and cells reverse direction. Recently, the FrzS S-motility protein was observed to localize predominantly to the piliated pole. In time, FrzS was redeployed to the opposite pole and its sequestration at the new site coincided with cell reversal. The C-terminal region of FrzS, a response regulator homolog, is rich in coiled-coil motifs and is required for dynamic localization and proper motility. These results raise the possibility that proper spatial control of FrzS has an important role in the regulation of cell reversal and S-motility.     

A Dynamic Response Regulator Protein Modulates G-Protein-Dependent Polarity in the Bacterium Myxococcus xanthus

Migrating cells employ sophisticated signal transduction systems to respond to their environment and polarize towards attractant sources. Bacterial cells also regulate their polarity dynamically to reverse their direction of movement. In Myxococcus xanthus, a GTP-bound Ras-like G-protein, MglA, activates the motility machineries at the leading cell pole. Reversals are provoked by pole-to-pole switching of MglA, which is under the control of a chemosensory-like signal transduction cascade (Frz). It was previously known that the asymmetric localization of MglA at one cell pole is regulated by MglB, a GTPase Activating Protein (GAP). In this process, MglB specifically localizes at the opposite lagging cell pole and blocks MglA localization at that pole. However, how MglA is targeted to the leading pole and how Frz activity switches the localizations of MglA and MglB synchronously remained unknown. Here, we show that MglA requires RomR, a previously known response regulator protein, to localize to the leading cell pole efficiently. Specifically, RomR-MglA and RomR-MglB complexes are formed and act complementarily to establish the polarity axis, segregating MglA and MglB to opposite cell poles. Finally, we present evidence that Frz signaling may regulate MglA localization through RomR, suggesting that RomR constitutes a link between the Frz-signaling and MglAB polarity modules. Thus, in Myxococcus xanthus, a response regulator protein governs the localization of a small G-protein, adding further insight to the polarization mechanism and suggesting that motility regulation evolved by recruiting and combining existing signaling modules of diverse origins.