共查询到20条相似文献,搜索用时 31 毫秒
1.
K M Liu C C Chang 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1986,10(2):79-85
The ultrastructure of lipofuscin granules in neuroglial cells of the optic nerve of the Formosan Rock-Monkey was investigated by electron microscopy. In the cytoplasm of astroglial cells, numerous irregular lipofuscin granules were characterized by the presence of large lipid droplets, small electron-dense pigment granules, and some lamellar structures. The lipofuscin granules of the oligodendroglial cells were composed largely of dense, coarse pigment granules, multilinear structures, and a few small lipid droplets. The lipofuscin granules in microglial cells were characterized by numerous lipid droplets in various sizes, small electron-dense pigment granules, and prominent lamellar structures. It was reported that the lipofuscin granules are wear-and-tear materials and products from the cells in lower functional activity. However, our observations suggest that the presence of lipofuscin granules in the neuroglial cells of the optic nerve is likely a characteristic product of active phagocytosis. 相似文献
2.
Occurrence of the autofluorescent pigment, lipofuscin, in polar crustaceans and its potential as an age marker 总被引:2,自引:0,他引:2
In crustaceans, the lack of reliable methods often prevents the determination of individual age. The quantification of the autofluorescent age pigment, lipofuscin, has revealed promising results in boreal and tropical species. We studied the presence of morphological lipofuscin and its possible application as an age marker in five Arctic and five Antarctic species, comprising decapods, amphipods and a euphausiid. Lipofuscin granules were located in the brain, using confocal fluorescence microscopy, and quantified from digital images. The pigment was found in 94 of 100 individuals and in all 10 species, and granules occurred in easily detectable amounts in 5 species. Two scavenging amphipod species, the Antarctic Waldeckia obesa and the Arctic Eurythenes gryllus, revealed the most conspicuous and numerous granules. There was a broad, though weak, correlation of lipofuscin concentration with individual body size within a species, but not with absolute body size of one species compared to another. In larvae of the decapod Chorismus antarcticus, lipofuscin accumulation was quantified over the 1st 4 months after larval release. Morphological lipofuscin is a potential index of age in those investigated species with a sufficient accumulation rate of the pigment. 相似文献
3.
A quantitative study of the lipofuscin content was carried out by image analysis in brains of known-age, pond-reared Penaeus japonicus (Crustacea, Decapoda) with the aim of assessing the applicability of the lipofuscin technique as an estimator of the physiological age in penaeids. With this purpose, three distinct measurements of lipofuscin levels (% area fraction, granule density and mean granule size) were recorded in ten sections of the olfactory lobe cell mass (OLCM) per animal. The image analysis was based on the autofluorescence emitted by the pigment, which accentuates the contrast between the lipofuscin granules and the background tissue. The concentration of lipofuscin increased significantly with age and was independent of sex. The relationship between age and lipofuscin concentration (area fraction and granule density) was best described by a seasonalized von Bertalanffy function, since the accumulation rate of the pigment dramatically slowed down in fall-winter, probably as a result of reduced seasonal metabolism. The present results confirm the potential of the lipofuscin method in the estimation of physiological age in penaeids and suggest that the application of this methodology can be useful in studies of age structure in wild populations and in the assessment of natural resources. J. Exp. Zool. 286:120-130, 2000. 相似文献
4.
A B Basson S E Terblanche W Oelofsen 《Comparative biochemistry and physiology. A, Comparative physiology》1982,71(3):369-374
1. A modified procedure, based on the autofluorescent properties of the age pigment, lipofuscin, has been applied to obtain quantitative data on the accumulation of the proteolipid fraction of the pigment in a variety of tissues of the rat (Rattus norvegicus). The tissues investigated included muscle (fast-twitch white and red fibers), heart, brain and spleen. 2. The effects of a life-long treadmill training program on the concentration of lipofuscin in the different tissues, was determined in a cross-sectional study at various ages between 41/2 and 19 months. 3. All the tissues investigated revealed an increase in the concentration of lipofuscin with training. The increase exceeded the increment observed in ageing control animals. 4. It is postulated that the elevated lipofuscin levels observed with physical training reflect an ultrastructural adaptive change in the cells. A relative deficiency of oxygen in the tissues could be the important metabolic factor triggering this mechanism. 相似文献
5.
Neurons of Aplysia californica are naturally pigmented and the pigment accumulates with age. In the present study the pigment was examined in the same neuron from Aplysia of three postmetamorphic ages: young, sexually mature, and old. The large central neuron, R2, was examined by light and electron microscopy to determine if the pigment possessed properties similar to lipofuscin pigment seen in aging mammalian neurons. We used the same microscopic techniques that demonstrate the presence of lipofuscin in mammalian neurons. Light microscopic studies demonstrated a regional correlation between autofluorescence, staining with Sudan Black, and the naturally occurring pigment in old R2s. Electron microscopic studies revealed the presence of large vacuolated and lamellated membrane-bound bodies in the peripheral cytoplasm of old R2s, similar to those found in mammalian neurons. The bodies were located in the same region in which autofluorescence and Sudan Black staining were observed. Although the naturally occurring pigment accumulates with age, it acquires characteristics of lipofuscin pigment in the neurons of older sexually mature animals. The presence of these pigment characteristics can be used as an index of aging in Aplysia neurons as they are in mammalian neurons. 相似文献
6.
The photoreactivity of the retinal age pigment lipofuscin. 总被引:5,自引:0,他引:5
J Wassell S Davies W Bardsley M Boulton 《The Journal of biological chemistry》1999,274(34):23828-23832
The presence of the age pigment lipofuscin is associated with numerous age-related diseases. In the retina lipofuscin is located within the pigment epithelium where it is exposed to high oxygen and visible light, a prime environment for the generation of reactive oxygen species. Although we, and others, have demonstrated that retinal lipofuscin is a photoinducible generator of reactive oxygen species it is unclear how this may translate into cell damage. The position of lipofuscin within the lysosome infers that irradiated lipofuscin is liable to cause oxidative damage to either the lysosomal membrane or the lysosomal enzymes. We have found that illumination of lipofuscin with visible light is capable of extragranular lipid peroxidation, enzyme inactivation, and protein oxidation. These effects, which were pH-dependent, were significantly reduced by the addition of the antioxidants, superoxide dismutase and 1,4-diazabicyclo(2,2,2)-octane, confirming a role for both the superoxide anion and singlet oxygen. We postulate that lipofuscin may compromise retinal cell function by causing loss of lysosomal integrity and that this may be a major contributory factor to the pathology associated with retinal light damage and diseases such as age-related macular degeneration. 相似文献
7.
The influence of lipofuscin granules and melanosomes from human retinal pigment epithelium on the light-induced photooxidation of cardiolipin liposomes and the generation of superoxide radicals was studied. Lipofuscin granules were able to stimulate, while melanosomes inhibited, the cardiolipin photooxidation. The visible light irradiation of both melanosomes and lipofuscin granules generated superoxide radicals with mean rates of 1.5 nmole/min/10(7) and 38 nmole/min/10(7) granules, accordingly. However, melanosomes but not lipofuscin granules reacted readily with superoxide radicals. Moreover, the rate constant of degradation of superoxide radicals in the presence of melanosomes was about five orders of magnitude higher than the rate constant of its photogeneration. Therefore, we propose that melanosomes in retinal pigment epithelium cells have a photoprotective role whereas lipofuscin granules may stimulate photodestructive reactions. 相似文献
8.
Majhi S Jena BS Patnaik BK 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2000,126(3):293-298
Oxidative damage was assessed through the estimation of lipid peroxides (LP) in the lungs of an ageing short-lived species of reptile, Calotes versicolor, commonly known as the garden lizard. Attempts were also made to trace its relationship with the age pigment, lipofuscin and the antioxidant ascorbic acid. While LP increased with advancing age the contents of both lipofuscin and ascorbic acid did not show appreciable change during maturation ( < 1-1 year old) but declined during senescence phase (1 to 2-4 year old). While the pattern of age associated changes in LP and ascorbic acid indicate similarity with the pattern observed in most of the mammals, the reduction of lipofuscin in older lizards is a significant departure from the common trend. 相似文献
9.
R. Hendy 《Histochemistry and cell biology》1971,26(4):311-318
Summary Morphology has hitherto been the sole criterion for the identification of lipofuscin pigment in the electron microscope. The Fontana and Schmörl reactions, two of the special stains used to characterise the pigment in the light microscope, produce electron-dense precipitates, and have been adapted for the ultrastructural confirmation of lipofuscin. The stains have been tested on pigment produced in cardiac muscle of rats on a vitamin E-deficient diet or on a diet containing high doses of the food colouring Brown FK. 相似文献
10.
Pattern of lipofuscin pigmentation in nitrergic and non-nitrergic,neurofilament immunoreactive myenteric neuron types of human small intestine 总被引:5,自引:5,他引:0
Lipofuscin, an autofluorescent age pigment, occurs in enteric neurons. Due to its broad excitation and emission spectra, it overlaps with commonly used fluorophores in immunohistochemistry. We investigated the pattern of lipofuscin pigmentation in neurofilament (NF)-reactive nitrergic and non-nitrergic human myenteric neuron types. Subsequently, we tested two methods for reduction of lipofuscin-like autofluorescence. Myenteric plexus/longitudinal muscle wholemounts of small intestines of five patients undergoing surgery for carcinoma (aged between 18 and 69 years) were double stained for NF and neuronal nitric oxide synthase (nNOS). Lipofuscin pigmentation patterns were semiquantitatively evaluated by using confocal laser scanning microscopy with three different excitation wave lengths (one for undisturbed lipofuscin autofluorescence and two for specific labellings). Two pigmentation patterns could be detected in the five NF-reactive neuron types investigated. In nitrergic/spiny as well as in non-nitrergic/stubby neurons, coarse, intensely autofluorescent pigment granules were prominent. In non-nitrergic type II, III and V neurons, a fine granular, diffusely distributed and less intensely autofluorescent pigment was obvious. After incubation of wholemounts in either CuSO4 or Sudan black B solutions, unspecific autofluorescence could be substantially reduced whereas specific NF and nNOS fluorescence remained largely unaffected. We conclude that NF immunohistochemistry is useful for morphological representation of subpopulations of human myenteric neurons. The lipofuscin pigmentation in human myenteric neurons reveals at least two different patterns which can be related to distinct neuron types. Incubations of multiply stained whole mounts in both CuSO4 or Sudan black B are suitable methods for reducing autofluorescence thus facilitating discrimination between specific (immunohistochemical) and non-specific (lipofuscin) fluorescence. 相似文献
11.
C. E. Graham 《The Histochemical journal》1970,2(6):521-525
Synopsis The body-wide distribution of a pigment present in many organs of squirrel monkeys (Saimiri sciurea) is described. The pigment, which was identified by histochemical techniques as lipofuscin, occurred intracellularly in some organs, and extracellularly in others. 相似文献
12.
Summary The effect of ageing on adrenergic neurons was studied in the hypogastric ganglion of the male rat using the formaldehyde-induced fluorescence method.With age, two changes were obvious. Firstly, the fluorescence intensity of the neurons decreased throughout postnatal life and the number of completely non-fluorescent adrenergic neurons increased. Secondly, the amount of non-specific fluorescence due to lipofuscin pigment increased. The pigment fluorescence was also found around the neurons in satellite cells. 相似文献
13.
Zsolt Ablonczy Daniel Higbee Angus C. Grey Yiannis Koutalos Kevin L. Schey Rosalie K. Crouch 《Archives of biochemistry and biophysics》2013
The accumulation of lipofuscin in the retinal pigment epithelium (RPE) has been implicated in the development of age-related macular degeneration (AMD) in humans. The exact composition of lipofuscin is not known but its best characterized component is N-retinylidene-N-retinylethanolamine (A2E), a byproduct of the retinoid visual cycle. Utilizing our recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI–IMS)-based technique to determine the spatial distribution of A2E, this study compares the relationships of lipofuscin fluorescence and A2E in the murine and human RPE on representative normal tissue. To identify molecules with similar spatial patterns, the images of A2E and lipofuscin were correlated with all the individual images in the MALDI–IMS dataset. In the murine RPE, there was a remarkable correlation between A2E and lipofuscin. In the human RPE, however, minimal correlation was detected. These results were reflected in the marked distinctions between the molecules that spatially correlated with the images of lipofuscin and A2E in the human RPE. While the distribution of murine lipofuscin showed highest similarities with some of the known A2E-adducts, the composition of human lipofuscin was significantly different. These results indicate that A2E metabolism may be altered in the human compared to the murine RPE. 相似文献
14.
Excessive accumulation of lipofuscin in postmitotic retinal pigment epithelial cells is a common pathogenetic pathway in various blinding retinal diseases including age-related macular degeneration, which is now the most common cause of registerable blindness in the industrialized nations. To better understand the role of lipofuscin accumulation and to manipulate the pathogenetic mechanisms on both experimental and therapeutic levels we analyzed the proteome of isolated human ocular lipofuscin granules from human RPE cells. After homogenization and fractionation by gradient ultracentrifugation of the RPE/choroid complex from 10 pairs of human donors, protein compounds were separated by 2D gel electrophoresis and analyzed using matrix-assisted laser desorption/ionization mass spectrometry and HPLC-coupled electrospray tandem mass spectrometry. Besides a better understanding of downstream pathways, this approach may provide new targets for therapeutic interventions in a currently untreatable disease. 相似文献
15.
G. Ettershank 《Polar Biology》1985,4(4):199-201
Summary Further results are reported on the age structure of populations of the Antarctic krill, using the age pigment lipofuscin. Study of the males shows a five-year adult life span as in females in an earlier study. Only a single age group was found in the juvenile stage. 相似文献
16.
Autofluorescence method to measure macular pigment optical densities fluorometry and autofluorescence imaging 总被引:3,自引:0,他引:3
Delori FC 《Archives of biochemistry and biophysics》2004,430(2):156-162
Non-invasive measurement of the optical density of the human macular pigment by the autofluorescence method takes advantage of the fluorescence of lipofuscin in the human retinal pigment epithelium. Measuring the intensity of fluorescence above 550 nm, where macular pigment has essentially zero absorption, and stimulating the fluorescence with two wavelengths, one well absorbed by macular pigment and the other minimally absorbed by macular pigment, provides a single-pass measurement of the macular pigment optical density. The method is implemented either by fluorometry of lipofuscin to yield the optical density of the macular pigment in a 2 degrees diameter central area, or by autofluorescence imaging to yield a high-resolution map of the macular pigment distribution. 相似文献
17.
Ottis P Koppe K Onisko B Dynin I Arzberger T Kretzschmar H Requena JR Silva CJ Huston JP Korth C 《Proteomics》2012,12(15-16):2445-2454
The accumulation of an autofluorescent pigment called lipofuscin in neurons is an invariable hallmark of brain aging. So far, this material has been considered to be waste material without particular relevance for cellular pathology. However, two lines of evidence argue that lipofuscin may play a yet unidentified role for pathological cellular functions: (i) Genetic forms of premature accumulation of similar autofluorescent material in neuronal ceroid lipofuscinosis indicate a direct disease-associated link to lipofuscin; (ii) Retinal pigment epithelium cell lipofuscin is mechanistically linked to age-associated macular degeneration. Here, we purified autofluorescent material from the temporal and hippocampal cortices of three different human individuals by a two-step ultracentrifugation on sucrose gradients. For human brain lipofuscin, we could identify a common set of 49 (among > 200 total) proteins that are mainly derived from mitochondria, cytoskeleton, and cell membrane. This brain lipofuscin proteome was validated in an interspecies comparison with whole brain rat lipofuscin (total > 300 proteins), purified by the same procedure, yielding an overlap of 32 proteins (64%) between lipofuscins of both species. Our study is the first to characterize human and rat brain lipofuscin and identifies high homology, pointing to common cellular pathomechanisms of age-associated lipofuscin accumulation despite the huge (40-fold) difference in the lifespan of these species. Our identification of these distinct proteins will now allow research in disturbed molecular pathways during age-associated dysfunctional lysosomal degradation. 相似文献
18.
Retinal pigment epithelium pigment granules stimulate the photo-oxidation of unsaturated fatty acids
Alexander E. Dontsov Randolph D. Glickman Mikhail A. Ostrovsky 《Free radical biology & medicine》1999,26(11-12):1436-1446
The cellular pigments of the retinal pigment epithelium (RPE) have been shown to catalyze free radical activity, especially when illuminated with visible or ultraviolet light. This activity is sufficient to cause photooxidation of several major cellular components. The present investigation determined the relative ability of melanin, lipofuscin, and melanolipofuscin granules isolated from human and bovine eyes to oxidize polyunsaturated fatty acids, specifically linoleic and docosahexaenoic acids. The dark reactivity as well as the light-stimulated reactions were determined. The production of hydroperoxide derivatives of the linoleic and docosahexaenoic acids were determined by NADPH oxidation coupled to the activity of glutathione peroxidase, and also by production of thiobarbituric acid reactive substances. All RPE pigment granules stimulated fatty acid oxidation when irradiated with short wavelength (< 550 nm) visible light, with the melanosomes exhibiting the greatest light-induced activity. Only lipofuscin granules, however, caused peroxidation of fatty acids in the dark. These findings provide additional support for the role of RPE pigments in "blue light toxicity" as well as indicating that accumulation of lipofuscin may contribute to increased photooxidation in the aging RPE. 相似文献
19.
We have observed pigmented cytoplasmic granules, with the characteristic staining properties of lipofuscin (ceroid, "wear-and-tear") pigment, in newborn human liver. The pigment is found at the periphery of the lobule in hepatocytes and some bile ductular cells. It is acid-fast, PAS-positive after diastase digestion, slightly argyophilic and sudanophilic, and markedly Schmorl's- and peroxidase positive in paraffin sections. Difficult to see in sections stained with hematoxylin and eosin, the pigment can be detected in unstained sections. The granules also resemble lipofuscin found in adult tissues, in their ultra-structural and enzymatic properties. They are polymorphic, contain granular material of moderate and high electron opacity, and are delimited by a single membrane. Acid phosphatase and β-glucuronidase activities are visualized in the newborn granules, identifying them as lysosomes. The granules also contain copper and, to a much lesser extent, iron. The accumulation of lipofuscin pigment in lysosomes in many tissues correlates well with aging, and this process has been interpreted as a reflection of cellular degeneration or wear-and-tear. However, the presence of lipofuscin granules as a constant component of neonatal liver suggests that they are not a measure of cellular senescence. 相似文献
20.
The number of neurocytes containing lipofuscin pigment (LEP) was determined in the nucleus infundibularis dorsalis (NID), the subfornical organ (SFO) and the medulla oblongata (MO) of the brain of sexually mature females and males of Rana temporaria L. approximately identical in length and age. The frogs were captured in their natural habitat in seven periods of the annual cycle. The number of LFP-positive neurocytes shows distinct seasonal variations in the analyzed areas. In general, neurocytes containing LFP were found in higher numbers during the winter months, and their significant drop was observed in summer. The results seem to suggest that cells containing LFP represent a certain kind of dynamic cellular system responding to various internal and/or external factors changing within a year. 相似文献