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1.
Roh SW Kim KH Nam YD Chang HW Kim MS Yoon JH Oh HM Bae JW 《Journal of microbiology (Seoul, Korea)》2008,46(5):525-529
A novel bacterium B9T was isolated from tidal flat sediment. Its morphology, physiology, biochemical features, and 16S rRNA gene sequence were
characterized. Colonies of this strain are yellow and the cells are Gram-negative, rod-shaped, and do not require NaCl for
growth. The 16S rRNA gene sequence similarity indicated that strain B9T is associated with the genus Lysobacter (≤ 97.2%), Xanthomonas (≤ 96.8%), Pseudomonas (≤ 96.7%), and Luteimonas (≤ 96.0%). However, within the phylogenetic tree, this novel strain shares a branching point with the species Luteimonas composti CC-YY255T (96.0%). The DNA-DNA hybridization experiments showed a DNA-DNA homology of 23.0% between strain B9T and Luteimonas mephitis B1953/27.1T. The G+C content of genomic DNA of the type strain is 64.7 mol% (SD, 1.1). The predominant fatty acids are iso-C11:0, iso-C15:0, iso-C16:0, iso-C17:0, iso-C17:0
ω9c, and iso-C11:0 3-OH. Combined analysis of the 16S rRNA gene sequences, fatty acid profile, and results from physiological and biochemical
tests indicated that there is genotypic and phenotypic differentiation of the isolate from other Luteimonas species. For these reasons, strain B9T was proposed as a novel species, named Luteimonas aestuarii. The type strain of the new species is B9T (= KCTC 22048T, DSM 19680T). 相似文献
2.
Kim KK Kim YO Park S Kang SJ Nam BH Kim DN Oh TK Yoon JH 《Journal of microbiology (Seoul, Korea)》2011,49(3):381-386
A Gram-staining-negative, motile, non-spore-forming and rod-shaped bacterial strain, 20-23RT, was isolated from intestine of bensasi goatfish, Upeneus bensasi, and its taxonomic position was investigated by using a polyphasic study. Phylogenetic analyses based on 16S rRNA gene sequences
revealed that strain 20-23RT belonged to the genus Shewanella. Strain 20-23RT exhibited 16S rRNA gene sequence similarity values of 99.5, 99.2, and 97.5% to Shewanella algae ATCC 51192T, Shewanella haliotis DW01T, and Shewanella chilikensis JC5T, respectively. Strain 20-23RT exhibited 93.1–96.0% 16S rRNA gene sequence similarity to the other Shewanella species. It also exhibited 98.3–98.4% gyrB sequence similarity to the type strains of S. algae and S. haliotis. Strain 20-23RT contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones
were Q-8 and Q-7. The fatty acid profiles of strain 20–23RT, S. algae KCTC 22552T and S. haliotis KCTC 12896T were similar; major components were iso-C15:0, C16:0, C16:1 ω7c and/or iso-C15:0 2-OH and C17:1 ω8c. The DNA G+C content of strain 20-23RT was 53.9 mol%. Differential phenotypic properties and genetic distinctiveness of strain 20–23RT, together with the phylogenetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain 20-23RT represents a novel species of the genus Shewanella, for which the name Shewanella upenei sp. nov. is proposed. The type strain is 20–23RT (=KCTC 22806T =CCUG 58400T). 相似文献
3.
Taxonomic studies were performed on three strains isolated from Cheonho reservoir in Cheonan, Korea. The isolates were Gram-negative,
aerobic, rod-shaped, non-motile, catalase-positive, and oxidase-positive. Colonies on solid media were cream-yellow, smooth,
shiny, and circular. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belong to the genus
Flavobacterium. The strains shared 98.6–99.4% sequence similarity with each other and showed less than 97% similarity with members of the
genus Flavobacterium with validly published names. The DNA-DNA hybridization results confirmed the separate genomic status of strains ARSA-42T, ARSA-103T, and ARSA-108T. The isolates contained menaqui-none-6 as the predominant menaquinone and iso-C15:0, iso-C15:0 3-OH, iso-Ci15:1 G, and iso-C16:0 3-OH as the major fatty acids. The genomic DNA G+C content of the isolates were 31.4–33.2 mol%. According to the phenotypic
and genotypic data, these organisms are classified as representative of three novel species in the genus Flavobacterium, and the name Flavobacterium koreense sp. nov. (strain ARSA-42T =KCTC 23182T =JCM 17066T =KACC 14969T), Flavobacterium chungnamense sp. nov. (strain ARSA-103T =KCTC 23183T =JCM 17068T =KACC 14971T), and Flavobacterium cheonanense sp. nov. (strain ARSA-108T =KCTC 23184T =JCM 17069T =KACC 14972) are proposed. 相似文献
4.
Bouraoui H Aissa MB Abbassi F Touzel JP O'donohue M Manai M 《Archives of microbiology》2012,194(5):315-322
An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated
from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23T, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic
analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23T is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas
chromatography were iso-C15:0, iso-C17:0 and iso C17:1 ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA–DNA hybridization measurements revealed
low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23T represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23T (=DSM 18496T; LMG 23756T). 相似文献
5.
A novel bacterial strain, designated T-Y1T, capable of degrading a variety of polysaccharides was isolated from seawater of an oyster farm in the South Sea, Korea. It was found to be aerobic, Gram-negative, non-flagellated, non-gliding and rod-shaped. Strain T-Y1T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain T-Y1T belonged to the genus Winogradskyella. Strain T-Y1T exhibited 16S rRNA gene sequence similarity values of 95.0–96.8 % to the type strains of recognized Winogradskyella species and less than 94.5 % to other validly named species. The chemotaxonomic data concurred with the phylogenetic inference. Strain T-Y1T contained MK-6 as the predominant menaquinone and anteiso-C15:0, iso-C15:0, iso-C15:1 G and iso-C16:0 3-OH as the major fatty acids. The major polar lipids of strain T-Y1T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 36.2 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, enabled strain T-Y1T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain T-Y1T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella multivorans sp. nov. is proposed. The type strain is T-Y1T (=KCTC 23891T = CCUG 62216T). 相似文献
6.
Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater
A novel Gram-negative, rod shaped, motile, non-sporing strictly aerobic bacterium, designated strain AK5T, was isolated from a sea water sample collected near Visakhapatnam coast, Bay of Bengal, India. Colonies on marine agar were
circular, 3–4 mm in diameter, creamish and rose with entire margin. Growth occurred at 10–40°C, 0.5–12% (w/v) NaCl and pH
of 7–11. Strain AK5T was oxidase and catalase positive. The fatty acids were dominated by iso-branched saturated and unsaturated fatty acids with
a high abundance of iso-C15:0, iso-C17:0 and summed feature 9 (as defined by MIDI). Q8 was found to be the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol,
phosphatidylethanolamine and four unidentified phospholipids as polar lipids. The DNA G+C content of strain AK5T was 54.7 ± 0.2 mol%. Phylogenetic analysis revealed that strain AK5T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis with a phylogenetic distance of 5.3% (94.7% 16S rRNA gene sequence similarity) and clustered with the same species. Results
from the polyphasic taxonomy study support the conclusion that strain AK5T represents a novel Aliidiomarina species, for which the name Aliidiomarina haloalkalitolerans sp. nov. is proposed. The type strain of A. haloalkalitolerans is AK5T (= MTCC 11064T = JCM 17359T). 相似文献
7.
Byung-Chun Kim Kang Hyun Lee Mi Na Kim Min Young Jung Young-Hyo Chang Jongtae Lee Kee-Sun Shin 《Current microbiology》2011,62(2):606-611
Strain M1-2T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic
taxonomic approach. Strain M1-2T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from
4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5T). The 16S rRNA gene sequence similarity level between M1-2T and J. marina En5T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2T contains MK-6 as the major menaquinone and iso-C15:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2OH) and iso-C17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2T (= KCTC 23194T = NCAIM B.002413T). 相似文献
8.
A Gram-negative, aerobic, rod shaped, non-spore-forming bacterial strain, designated Dae08T, was isolated from sediment of the stream near Daechung dam in South Korea, and was characterized in order to determine its
taxonomic position, using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Dae08T belongs to the family Xanthomonadaceae of the Gammaproteobacteria, and is related to Lysobacter brunescens ATCC 29482T (97.3%). The phylogenetic distances from any other species with validly published names within the genus Lysobacter were greater than 3.7%. The G+C contents of the genomic DNA of strain Dae08T was 69.3 mol%. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with iso-C15:0, iso-C17:1, ω9c, iso-C17:0, iso-C16:0, and iso-C11:0 3-OH as the major acids supported the affiliation of strain Dae08T to the genus Lysobacter. DNA-DNA relatedness between strain Dae08T and its phylogenetically closest neighbour was 28%. On the basis of its phenotypic properties and phylogenetic distinctiveness,
strain Dae08T (= KCTC 12600T) should be classified in the genus Lysobacter as the novel species, for which the name Lysobacter daecheongensis sp. nov. is proposed. 相似文献
9.
Xing K Bian GK Qin S Klenk HP Yuan B Zhang YJ Li WJ Jiang JH 《Antonie van Leeuwenhoek》2012,101(2):433-441
A novel actinomycete, designated strain KLBMP 1111T, was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111T formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828T (98.8%), K. aridum subsp. largum DSM 44150T (98.1%) and Kibdelosporangium philippinense DSM 44226T (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H4); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol
and an unknown phospholipid; iso-C16:0, C16:0, anteiso-C15:0 and iso-C15:0 as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA–DNA hybridization values between strain KLBMP
1111T and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic
acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP
1111T represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111T (=KCTC 19775T = CCTCC AA 2010001T). 相似文献
10.
Yi-Guang Chen Shu-Kun Tang Yu-Qin Zhang Zhao-Yang Li Lang-Bo Yi Yong-Xia Wang Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(1):63-70
A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium,
designated strain JSM 078085T, was isolated from sea water collected from the South China Sea. Strain JSM 078085T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v)
NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan
type was A4α (l-Lys–l-Ala–l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085T contained menaquinone MK-9(H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major
polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 078085T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA
relatedness between strain JSM 078085T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data
supported the view that strain JSM 078085T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085T (=DSM 21081T=KCTC 19430T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078085T is EU583729. 相似文献
11.
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, JW-64-1T, capable of degrading methamidophos was isolated from a methamidophos-manufacturing factory in China, and was subjected to
a polyphasic taxonomic investigation. Strain JW-64-1T produced circular, smooth, transparent, yellow-colored colonies (1.0–2.0 mm) on LB agar after 2 days incubation. It grew
optimally at 25–30°C and pH 7.0 without the presence of NaCl. The G+C content of the total DNA was 63.6 mol%. A phylogenetic
analysis based on 16S rRNA gene sequences showed that strain JW-64-1T fell within the cluster comprising Luteibacter species. The 16S rRNA gene sequence of strain JW-64-1T was most closely related to Luteibacter rhizovicinus DSM 16549T (98.6%), followed by Luteibacter
yeojuensis DSM 17673T (98.4%) and L. anthropi CCUG 25036T (98.2%). The major cellular fatty acids of strain JW-64-1T were iso-C15:0 (24.1%), iso-C17:0 (20.2%) and summed feature 9 comprising iso-C17:1 ω9c and/or C16:0 10-methyl (20.3%). The major isoprenoid quinine was Q-8 (98%), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
phosphoaminolipid, aminolipids-1, aminolipids-2, and phospholipids. The values for DNA–DNA relatedness between strain JW-64-1T and the closest phylogenetic relatives of L. rhizovicinus and Luteibacter
yeojuensis were 34.8 ± 2.6 and 25.6 ± 3.1%, respectively. On the basis of the phenotypic, chemotaxonomic, DNA–DNA relatedness and phylogenetic
analysis based on the 16S rRNA gene sequences, strain JW-64-1T represents a novel species of the genus Luteibacter, for which the name Luteibacter jiangsuensis sp. nov. is proposed. The type strain is JW-64-1T (=CGMCC 1.10133T = DSM 22396T). 相似文献
12.
You-Sung Oh Hyung-Yeel Kahng Young Sun Lee Byoung-Jun Yoon Sang-Bin Lim Jae Sung Jung Duck-Chul Oh Dong-Heon Lee 《Journal of microbiology (Seoul, Korea)》2010,48(4):433-437
A strictly aerobic, red-pigmented, non-motile, catalase- and oxidase-positive, Gram-staining-negative bacterium, designated
strain CNURIC011T, was isolated from seawater off the coast of Jeju Island in Korea. A phylogenetic analysis based on 16S rRNA gene sequences
showed that strain CNURIC011T belongs to the genus Aquimarina in the family Flavobacteriaceae. 16S rRNA gene sequence analysis revealed that the close relatives of the novel strain are Aquimarina latercula ATCC 23177T, Aquimarina marcrocephali JAMB N27T, Aquimarina intermedia KMM 6258T, Aquimarina muelleri KMM 6020T, and Aquimarina brevivitae SMK-19T, with sequence similarities of 97.6, 96.6, 96.0, 95.6, and 94.2%, respectively. DNA-DNA hybridization revealed that the level
of relatedness between strain CNURIC011T and Aquimarina latercula ATCC 23177T (=KCTC 2912T) was 4.9%. The DNA G+C content was 35.8 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C15:0 (14.9%), C15:0 (13.9%), iso-C17:0 3-OH (12.6%), iso-C15:1 G (7.3%), and iso-C17:1
ω9c (7.2%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC011T represents a novel species within the genus Aquimarina, for which the name Aquimarina litoralis sp. nov. is proposed. The type strain is CNURIC011T (=KCTC 22614T =JCM 15974T). 相似文献
13.
A non-motile red-pigmented bacterium, designated strain HMD1002T, was isolated from an artificial lake located on the campus of Hankuk University of Foreign Studies, South Korea. The major
fatty acids were iso-C15:0 (29.6%), Summed Feature 3 (comprising C16:1
ω7c and/or iso-C15:0 2-OH; 17.5%) and iso-C17:0 3-OH (12.5%). The major isoprenoid quinone was menaquinone-7 (MK-7). The DNA G+C content was 41.0 mol%. A phylogenetic tree
based on 16S rRNA gene sequences showed that strain HMD1002T formed a lineage in the genus Pedobacer and was closely related to Pedobacer terrae (96.3%) and Pedobacer suwonensis (95.8%) in sequence similarity. On the basis of the evidence presented in this study, strain HMD1002T represents a novel species of the genus Pedobacter, for which the name Pedobacter yonginense sp.nov. is proposed. The type strain is HMD1002T (=KCTC 22721T = CECT 7544T). 相似文献
14.
Annukka E. Mäkinen Anna H. Kaksonen Jaakko A. Puhakka 《Extremophiles : life under extreme conditions》2009,13(3):505-510
A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70BT was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50–81°C (optimum
71°C) and 6.2–9.8 (optimum pH 7–7.5), respectively. Growth occurred in the presence 0–2% NaCl (optimum 1% NaCl). Strain 70BT could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used
as electron acceptor. Major whole-cell fatty acids were iso-C15:0, C16:0 DMA (dimethyl acetal), C16:0 and anteiso-C15:0. The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives
of strain 70BT were Thermosediminibacter oceani DSM 16646T (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70BT represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70BT (=DSM 21563 = JCM 15541). 相似文献
15.
A bacterial strain, designated KMM 6244T, was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and
spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus
Bacillus. The nearest neighbor of strain KMM 6244T was Bacillus decolorationis LMG 19507T with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA–DNA hybridization experiments revealed a low relatedness (37%) of the
novel isolate with the type strain of B. decolorationis LMG 19507T. Strain KMM 6244T grew at 4–45°C and with 0–12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The
predominant fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, C15:0, iso-C16:0 and iso-C14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that
strain KMM 6244T represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244T (KCTC 12718T = LMG 26357T). 相似文献
16.
Qing-Zhong Peng Qing-Jing Peng Yu-Qin Zhang Zhu-Xiang Liu Yong-Xia Wang Wen-Jun Li Xiao-Long Cui Yi-Guang Chen 《Antonie van Leeuwenhoek》2009,96(4):497-504
A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM
071077T, was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Cells were motile by
means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071077T was able to grow with 2–25% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 7.5) and 10–40°C (optimum, 25–30°C).
meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the major cellular
fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that
strain JSM 071077T should be assigned to the genus Halobacillus, being related most closely to the type strain of Halobacillus
naozhouensis (98.8% sequence similarity), and the two strains formed a distinct subline in the neighbor-joining, minimum-evolution and
maximum-parsimony phylogenetic trees. The sequence similarities between the novel isolate and the type strains of other recognized
Halobacillus species ranged from 97.6% (with Halobacillus
alkaliphilus) to 95.2% (with Halobacillus kuroshimensis). The results of the phylogenetic analyses, combined with DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic
information, support that strain JSM 071077T represents a new species of the genus Halobacillus, for which the name Halobacillus hunanensis sp. nov. is proposed. The type strain is JSM 071077T (=DSM 21184T = KCTC 13235T). 相似文献
17.
You-Sung Oh Sang-Bin Lim Hyung-Yeel Kahng Byoung-Jun Yoon Duck-Chul Oh Jae-Ho Joa Dong-Heon Lee 《Journal of microbiology (Seoul, Korea)》2010,48(3):307-311
A novel marine bacterium, designated strain CNURIC014T was isolated from coastal seawater of Jeju Island in Korea. Strain CNURIC014T formed yellow colonies on marine agar 2216 and the cells were Gram-negative, non-motile, strictly aerobic, rod-shaped. The
temperature, pH and NaCl ranges for growth were 15–37°C, pH 6.0–9.0 and 1.0–7.0% NaCl. Phylogenetic analysis based on 16S
rRNA gene sequences indicated that strain CNURIC014T was most closely related to Gaetbulibacter marinus and Gaetbulibacter saemankumensis, with a sequence similarity of 95.1% and 94.6%, respectively. The DNA G+C content of the strain was 33.1 mol% and the major
respiratory quinone was menaquinone-6. The major cellular fatty acids were iso-C15:1 (22.8%), iso-C15:0 (18.8%), summed feature 3 (iso-C15:0 2-OH/C16:1 ω7c, 12.9%) and iso-C17:0 3-OH (11.5%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC014T represents a novel species within the genus Geatbulibacter, for which the name Gaetbulibacter jejuensis sp. nov. is proposed. The type strain is CNURIC014T(=KCTC 22615T =JCM 15976T). 相似文献
18.
Strain AK12T, an orange pigmented Gram-negative, rod shaped, non-motile bacterium, was isolated from a mud sample collected from a brackish
water pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant
fatty acids were iso-C15:0 (42.7%), iso-C17:0 3OH (13.2%), C16:1ω7c/C16:1ω6c (summed feature 3) (8.0%), iso-C17:1 I/anteiso-C17:1 B (summed feature 4) (6.1%) and iso-C17:1ω9c/C16:0 10-methyl (summed feature 9) (9.4%). Strain AK12T contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six
unidentified lipids as the polar lipids. The G + C content of DNA of the strain AK12T was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12T was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain
AK12T clustered with E. vietnamensis and together with E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the
current polyphasic study, strain AK12T is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12T (= MTCC 11083T = JCM 17847T). 相似文献
19.
A Gram-negative, motile and rod-shaped bacterial strain, designated S7-3T, was isolated from a tidal flat sediment at Saemankum on the western coast of Korea. Phylogenetic analyses based on 16S rRNA gene and gyrB sequences showed that strain S7-3T belonged to the genus Shewanella, clustering with Shewanella decolorationis S12T. Strain S7-3T exhibited 98.8 % 16S rRNA gene sequence similarity and 96.8 % gyrB sequence similarity to S. decolorationis S12T, respectively. The 16S rRNA gene sequence similarity values between strain S7-3T and other members of the genus Shewanella were in the range of 93.0–98.0 %. Strain S7-3T contained simultaneously both menaquinones (MK) and ubiquinones (Q); the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The fatty acid profiles of strain S7-3T and S. decolorationis JCM 21555T were similar; major components were C17:1 ω8c, iso-C15:0 and iso-C15:0 2-OH and/or C16:1 ω7c. The DNA G+C content of strain S7-3T was 51.8 mol% and its mean DNA–DNA relatedness value with S. decolorationis JCM 21555T was 43 %. Differential phenotypic properties of strain S7-3T, together with the phylogenetic and genetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain S7-3T is considered to represent a novel Shewanella species, for which the name Shewanella seohaensis sp. nov. is proposed. The type strain is S7-3T (=KCTC 23556T = CCUG 60900T). 相似文献
20.
A bacterial strain designated antisso-27T, previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using
the polyphasic taxonomic approach. Strain antisso-27T was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based
on 16S rRNA gene sequences showed that strain antisso-27T belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0–96.6% to other valid Aquimarina spp. It contained iso-C17:0 3-OH, iso-C15:0, iso-C16:0, iso-C15:1 and iso-C15:0 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone.
Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized
phospholipids. Strain antisso-27T employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain
antisso-27T should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27T (= BCRC 80080T = LMG 25375T). 相似文献