Lecithostaphylus retroflexus (Molin, 1859) (Zoogonidae) and Tergestia acanthocephala (Stossich, 1887) (Fellodistomidae) (Digenea) from the epipelagic teleost Belone belone (L.) in the western Mediterranean

Numerous individuals of the poorly known species Lecithostaphylus retroflexus (Zoogonidae) and Tergestia acanthocephala (Fellodistomidae) have been recovered from the teleost fish Belone belone gracilis from off the Scandola Nature Reserve, Western Mediterranean. They are redescribed, incorporating previously undescribed features: for L. retroflexus, a post-oral ring, a bipartite seminal vesicle, the shape of the excretory vesicle, the subterminal excretory pore and the flask-shaped gland-cells associated with the distinctly pedunculate ventral sucker; and for T. acanthocephala, the intestinal bifurcation in the forebody, necessitating its return to the genus Tergestia from Theledera. Additionally, T. acanthocephala is compared with T. laticollis from various species of Trachurus from the same geographical area.     

Interspecific competition among larvae ofHemipyrellia ligurriens (Calliphoridae) andBoettcherisca formosensis (Sarcophagidae) (Diptera)

1. The effects of larval rearing density and species relative proportions on life-history parameters of two necrophagous Diptera, Hemipyrellia ligurriens (Wiedemann) (Calliphoridae) and Boettcherisca formosensis Kirner and Lopes (Sarcophagidae), were investigated in mixed cultures. Larval rearing density had a significant effect on larval to adult survivorship, duration of immature development, adult size and relative performance (measured by the composite index of performance, r′) of both species. However, species relative proportions affected adult size of both flies and the duration of immature development of B. formosensis only.
2. B. formosensis had a higher survivorship than H. ligurriens in all mixed cultures and showed a similar survivorship pattern to that in pure cultures. By contrast, survivorship of H. ligurriens was lower in mixed than in pure cultures.
3. H. ligurriens adults reared from mixed cultures were smaller than those from pure cultures of comparable density, but B. formosensis adults from pure and mixed cultures were of similar size.
4. The results suggest that competition between B. formosensis and H. ligurriens larvae was asymmetric and the former was the superior competior.
5. At low larval densities in mixed cultures, the presence of H. ligurriens enhanced the performance (as measured by r′) of B. formosensis, a consequence of suspected interspecific facilitation of larval growth.

     

Three new mermithids (Nematoda) from Guatemalan blackflies (Diptera: Simuliidae)

Summary The following new species of Mermithidae (Nematoda) are described from Guatemalan blackflies: (a) Gastromermis cloacachilus from Gigantodax wrighti; (b) Isomernis vulvachila from Cnephia pacheco-lunai and (c) Mesomermis guatemalae from Simulium metallicum. All parasites were collected at Encuentros (Department of Totonicapan) in Guatemala. These are the first representatives of Gastromermis and Mesomermis from Central America and M. guatemalae represents the second mermithid species to be described from an American vector of Onchocerca volvulus, causal agent of river blindness. ac]19800728     

Molecular evidence for the occurrence of Contracaecum rudolphii A (Nematoda: Anisakidae) in shag Phalacrocorax aristotelis (Linnaeus) (Aves: Phalacrocoracidae) from Sardinia (western Mediterranean Sea)

Specimens of Contracaecum rudolphii Hartwich, 1964 (Nematoda: Anisakidae) from Phalacrocorax aristotelis (Linnaeus) from the Archipelago of La Maddalena (Sardinia, western Mediterranean Sea) were characterised genetically and compared with C. rudolphii A sensu D’Amelio et al. 1990 and C. rudolphii B sensu D’Amelio et al. 1990 from Phalacrocorax carbo sinensis (Blumenbach) from north-eastern Italy, and with C. rudolphii C sensu D’Amelio et al. 2007 from Phalacrocorax auritus (Lesson) from west-central Florida, USA. The sequencing of the small subunit of the mitochondrial ribosomal RNA gene (rrnS) and by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis of the same gene and of the internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA) allowed the identification of all specimens of C. rudolphii from P. aristotelis as C. rudolphii A. The results confirmed that the definition of genetic markers, following the analysis of nuclear ribosomal and mitochondrial DNA, provides quick and practical diagnostic tools for the detection of the 3 sibling species of C. rudolphii. The occurrence of C. rudolphii in P. aristotelis is reported for the first time from the Mediterranean area, improving the picture of the dispersal patterns of the populations of these piscivorous birds, and confirming the existence of different and isolated populations between the North and South European waters.     

Analysis of multiparasitism by Eupelmus vuilleti (Craw) (Eupelmidae) and Dinarmus basalis (Rond) (Pteromalidae) in the presence of one of their common hosts,Bruchidius atrolineatus (Pic) (Coleoptera Bruchidae)

Bruchidius atrolineatus (Pic) is a tropical beetle (Coleoptera Bruchidae) that develops during the larval and pupal stages in the seeds of a legume Vigna unguiculata (Walp). Two species of Hymenoptera, Dinarmus basalis (Rond) and Eupelmus vuilleti (Craw), solitary ectoparasitoids of the larvae and pupae of B. atrolineatus, were introduced successively in the presence of their hosts, varying the interval between the two introductions. When D. basalis females were introduced 24 h, 3 days or 7 days after E. vuilleti, multiparasitism was low. The females had low fecundity, and their eggs were not distributed randomly over the different available hosts. When E. vuilleti females were introduced second, they oviposited on the different hosts availabe and did not avoid multiparasitism. The presence of hosts already parasitised by D. basalis increased the reproduction of E. vuilleti, and the fecundity of the females was higher than in control batches with E. vuilleti alone. E. vuilleti seems capable of detecting the ovipositor shafts drilled by the D. basalis females, and by introducing its own ovipositors killing the D. basalis eggs or larvae. When interspecific competition was occurring the number of E. vuilleti adults emerging from the seeds was no different from that observed in control batches with E. vuilleti alone, and there were always fewer D. basalis adults than in control batches (D. basalis alone). This interspecific competition reduces the influence of the two parasitoids in the biological control of bruchid populations.     

Species novae generisEremostachys (Labiatae)

Five new species are described:Eremostachys codonocalyx from NE. Iran (related toE. subspicata),E. stenocalycina from NE. Afghanistan (related toE. alberti andE. hissarica),E. salangensis from NE. Afghanistan (close toE. bamianica),E. freitagii from E. Afghanistan (similar but not closely related toE. vulnerans), andE. andersii from E. Afghanistan (similar but not closely related toE. vulnerans), andE. andersii from NE. Afghanistan of isolated systematic position.

Florae Iranicae praecursores 5–9.     

Proton-NMR study of the interaction of trans-dichloro-diamine-platinum(II) with poly(I) and poly(I).poly(C)

The fixation of trans-(NH₃)₂Cl₂ Pt(II) to poly(I)·poly(C) at low r_b (< 0.05) leads to the formation of two complexed species. The major species (ca. 82% of bound platinum) involves coordination of platinum to a single hypoxanthine base, while the other species involves coordination of two hypoxanthine bases, which are either far apart on the same strand or on separate poly(I) strands, to the platinum. These same two species are found after reaction with poly(I), as are two other species throughout the entire r_b range studied (r_b = 0–0.30). The latter two species are assigned to trans-Pt bound to two bases on a poly(I) strand with (a) one or (b) two free bases between the two bound bases. These two species, (a) and (b), account for ca. 35% of the bound platinum, although the 1:1 species remains dominant (ca. 55%). These two additional species are observed at high r_b (>0.075) after reaction with poly(I)·poly(C) but as very minor species. They are formed by reaction with melted poly(I) loops. Also at high r_b, we have observed a shifted cytidine H⁵ resonance arising from interaction of trans-Pt with a melted loop of poly(C). Most probably, this arises from an intramolecular poly(I) to poly(C) crosslink. Results from the reaction of trans-Pt with poly(C) are presented for comparison.     

Die Entwirrung desBotrytella(Sorocarpus)-Komplexes (Ectocarpaceae,Phaeophyta)

Sorocarpus Pringsh. is a later synonym forBotrytella Bory. Four species of the genus are known from northern European coasts. Three of them are found at Helgoland (North Sea), type locality of bothBotrytella uvaeformis (Pringsh.) andB. reinboldii (Reinke). A third species, most frequently seen, still remains unnamed. Plants of similar habit, however, have been described from Japan. The type species for the genus,Botrytella micromora Bory, was originally published asEctocarpus siliculosus β.uvaeformis by Lyngbye. A sample from Danish waters proved to be identical with it. In a previous paper (Kornmann & Sahling, 1984), the life history of two species was studied and the investigation is now extended to include the other two, demonstrating the conformity of the four species with the genus characters as to morphological and developmental features.      

Diplectanids (Monogenea) on the archerfish Toxotes jaculatrix (Pallas) (Toxotidae) off Peninsular Malaysia

Two new and two previously described species of diplectanid monogeneans (Heteroplectanum flabelliforme n. sp., Diplectanum sumpit n. sp., D. jaculator Mizelle & Kritsky, 1969 and D. toxotes Mizelle & Kritsky, 1969) were collected from archerfish Toxotes jaculatrix off the Island of Langkawi, Kedah and off Perak, Malaysia. The reproductive systems and squamodiscs of D. jaculator and D. toxotes are described for the first time. D. sumpit n. sp. differs from D. toxotes and D. jaculator in a having a small curved copulatory tube with a distinct accessory piece, compared to the long, tubular copulatory tube of D. jaculator and the slender tube of D. toxotes. D. sumpit n. sp. also differs from D. toxotes in having a larger ventral bar and larger squamodiscs. H. flabelliforme n. sp. differs from all known Heteroplectanum species in the shape and size of the squamodiscs, the arrangement of the sclerites in the squamodiscs, the extremely large ventral bar and the short, curved, non-spinous copulatory tube.     

Notes on Plecoptera (20) Protonemura towadensis (Kawai, 1954)

The genitalia of the male holotype of P. towadensis (from Honshu) are illustrated. The distinction from P. ermolenkoi Zhiltzova, 1982 (from Sachalin and the South Kurile Islands) is discussed.     

A taxonomic review of Phlebotomus (Idiophlebotomus) (Psychodidae)

Five known species (asperulus Quate & Fairchild, erebicolus Quate, pholetor Quate & Fairchild, sejunctus Quate, and stellae Quate) of the caverni-colous subgenus Phlebotomus (Idiophlebotomus) are discussed in relation to newly recognized species from West Malaysia and India. P. frondifer n.sp. and P. tubifer n.sp. are described and a key to the adults of all seven species is given. General features of the subgenus are discussed, with particular reference to the functional relationships between the specialized morphology of the mouthparts and the probable bat hosts of these species.     

Carnoya kermarreci n.sp. (Rhigonematidae: Nematoda) from Anadenobolus politus (Porat) (Rhinocricidae: Diplopoda) from Guadeloupe

Summary Carnoya kermarreci n.sp. (Rhigonematidae; Nematoda) is described from Anadenobolus politus (Porat) (Rhinocricidae; Diplopoda) from Guadeloupe. The new species most closely resembles C. martiniquensis Adamson, 1984 from A. politus in Martinique and C. venezuelensis Adamson, 1984 from Rhinocricus flavocinctus from Venezuela in that the lips in the male are broad and the arrangement of caudal papillae is almost identical in the three species. Males of C. kermarreci are easily distinguished by the absence of spines, by the form of the cuticular projections at the base of the buccal cavity and by the fact that the most anterior pair of caudal papillae are located very close together. Females are distinguished by the presence of three pairs of somatic papillae near the level of the vulva. The nematode fauna of A. politus includes species from two distinct orders and presumably reflects the fact that diplopods are among the most ancient hosts of nematodes.     

Archaeobotany of capers (Capparis) (Capparaceae)

The origins of capers, their use and cultivation are discussed. Capers seeds and charcoal are often recovered from archaeological sites of the Mediterranean and West Asia. These are referred to as C. Spinosa L. This is mostly a group of cultivars restricted to localities surrounding the Western Mediterranean and some places in the Eastern Mediterranean. Identification of the findings is discussed in terms of seed morphology, present distribution and ancient uses of C. aegyptia Lam., C. sicula Veill., C. cartilaginea Decne, C. orientalis Veill., C. decidua (Forssk.) Edgew. and other species. Citations of Capparis in early Rabbinic, Mesopotamian and Greco-Roman texts are presented. Received June 3, 2002 / Accepted October 8, 2002 Correspondence to: D. Rivera     

Biology of Nomioides variegatus (Olivier) (Hymenoptera: Halictidae)

The nesting biology, life history and pollen foraging of the minute Nomioides variegatus (Olivier) is described and illustrated. All Nomioides biological studies are reviewed. Nesting and foraging were observed over two years in a sandy natural area in the Rhône River delta region of southern France. Nest and cell structure was obtained from soil blocks removed from the nesting area and examined in the laboratory. Females, which were on average only 4.2 mm long, nested solitarily in loose aggregations in areas free of vegetation. Cells were placed from 6 to 12 cm deep, each terminating a lateral burrow off the main burrow. Cell walls were lined with a water proof wax-like lining. The pollen-nectar provision was a flattened sphere. The posterior end of the egg was embedded on the dorsal surface of the provision. Larval and pupal development was rapid, proceeding to adults by August, the over-wintering stage. Pollen foraging patterns were analyzed from both pollen provisions and fecal material for different nests (3) and cells (8). Nomioides variegatus is polylectic, but individuals bees exhibited a high degree of foraging consistency for the provisioning of single cells. Eleven different pollen types representing 10 plant families were found. In 1996, Ecballium elaterium (Cucurbitaceae) was the dominant pollen present in all samples but it was absent in 1997. Tamarix gallica (Tamaricaeae) was the dominant pollen type in 1997 and absent in 1996. A third pollen, Centaurium (Gentianaceae) was present in both years and represented 70% of some samples.     

Beschreibung einer neuen Carabus (Lamprostus)-Art aus Persien (Coleoptera: Carabidae)

Abstract

A new species of Carabus (Lamprostus) from the province of Gilan, Iran, is described and illustrated: Carabus (Lamprostus) staveni sp. nov., Carabus (Pachystus) rechingerae Mandl, 1972, is considered a junior synonym of Carabus (Lamprostus) calleyi nigrinus Motschulsky, 1865. The specific status of Carabus (Sphodristocarabus) korgei Heinz, 1970, is restored (stat. rev.) and Carabus separatus delirensis Deuve, 2000, is considered to be a junior synonym of it. Carabus (Megodontus) stroganowi javahardensis Deuve, 2000, is considered to be a synonym of Carabus (Megodontus) heinrichi Mandl, 1966; both taxa are from the same type locality.     

Neoechinorhynchus (Neoechinorhynchus) chimalapasensis n. sp. (Acanthocephala: Neoechinorhynchidae) from the freshwater fish Awaous banana (Valenciennes) (Gobiidae) in Mexico

Neoechinorhynchus (Neoechinorhynchus) chimalapasensis n. sp. (Eoacanthocephala: Neoechinorhynchidae) is described from the intestine of Awaous banana (Valenciennes) (Pisces: Gobiidae) collected in the Río Negro, a tributary in the upper Río Coatzacoalcos basin, Santa María Chimalapa, Oaxaca State, Mexico. It is the third species of Neoechinorhynchus Stiles & Hassall, 1905 described from Mexican freshwater fishes, although 36 other species are known from freshwater fishes in the Americas. Like four other species of Neoechinorhynchus from freshwater fishes in North America and Mexico, N. (N.) limi Muzzall & Buckner, 1982, (N.) rutili (Müller, 1780) Stiles & Hassall, 1905, N. (N.) salmonis Ching, 1984 and N. (N.) roseus Salgado-Maldonado, 1978, males and females of the new species are less than 20 mm in length, lack conspicuous sexual dimorphism in size, have a small proboscis of about 0.1 mm in length with the largest hooks being the anteriormost, about 30–90 μm in length and of equal size, and have subequal lemnisci, larger than the proboscis receptacle but still relatively short and, in males, generally restricted to a position considerably anterior to the testes. The new species is closest to N. (N.) roseus, but it is distinguished from it by having: (1) a slightly larger cylindrical proboscis with almost parallel sides versus a globular proboscis with a rounded tip which is shorter and somewhat wider in N. (N.) roseus; (2) smaller but robust anterior proboscis hooks that do not reach the equatorial level or extend beyond the hooks of the middle circle as in N. (N.) roseus; and (3) the female gonopore situated ventrally subterminal, as opposed to being a significant distance anteriorly to the posterior extremity in N. (N.) roseus.     

On the taxonomy and biogeography of Potamon atkinsonianum (Wood-Mason, 1871) and Potamon (Potamon) emphysetum (Alcock, 1909)

The Himalayas are known to be a geologically young and dynamic mountain range hosting an endemic flora and fauna. To date, for freshwater crabs, one endemic subgenus within the genus Potamon, Potamon (Himalayapotamon) Pretzmann, 1966, has been described from the Himalayas. This subgenus includes two species, Potamon atkinsonianum (Wood-Mason, 1871) and Potamon emphysetum (Alcock, 1909). The taxonomic position of these two species is reconsidered. The subgenus Himalayapotamon is raised to generic level and its systematic and zoogeographic position is discussed. Based mainly on the morphology of the male copulatory system, Himalayapotamon belongs to the family Potamidae. It is more related to the Eurasian genus Potamon Savigny, and is distinct from the South-East Asian genus Potamiscus as well as from the Indian freshwater crabs of the family Gecarcinucidae. Zoogeographically Himalayapotamon appears to be an endemic genus related to Potamon, both genera presumably being isolated during the Miocene when the Gangetic waters were separated from the western Eurasian river systems. Further speciation occurred during glacial periods.     

The large branchiopods (Anostraca,Notostraca and Spinicaudata) of Numidia (Algeria)

Between 1995 and 1999, we surveyed the large branchiopods (Crustacea, Branchiopoda) of Numidia, the coastal plain of northeastern Algeria. Samples from ca 100 sites yielded two species of Anostraca (Chirocephalus diaphanus, new to Numidia, and Tanymastix stagnalis), one notostracan (Lepidurus apus lubbocki) and one spinicaudatan (Cyzicus tetracerus). The absence of Streptocephalus torvicornis buchetiis noteworthy. An annotated check-list of all large branchiopods known from Algeria is also presented and discussed. Several species appear to be in danger of extinction.     

Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)

The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated.     

Immobilization of Cu(II) and Cr(IV) in basidiomycete-colonized sawdust

An investigation into the feasibility of removing Cu(II) and Cr(IV) from solution with basidiomycete (Gloeophylum sepiarium, Pleurotus sp.)-colonized sawdust was undertaken. Obeche (Triplochyton scleroxylon) sawdust exposed to the basidiomycetes for 1–3 months reduced the concentration of the metals in the solution to 22.0–84.4 mg/l. The supernatant from the centrifuged mixture of a solution of 100 mg metal ions/l and aqueous extract of a 3-month basidiomycete-degraded obeche sawdust contained lower concentration of the metal ions (38.6–75.4 mg/l). Unextracted sawdust of pigmented tropical timbers, African mahogany (Khaya ivorensis), black afara (Terminalia ivorensis) and camwood (Baphia nitida) exposed to the test basidiomycetes, removed Cu and Cr significantly better than the extracted sawdust. It is hypothesised that some products of basidiomycete wood-degradative activities were ligands which immobilized the test metals.