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1.
John Gorham 《Phytochemistry》1978,17(1):99-105
The growth inhibitory activity of lunularic acid and a number of analogues has been examined in liverwort gemmaling and cress root growth tests. Lunularic acid was no more active than a wide range of similar compounds and no clear correlation between structure and activity was observed. The effects of these compounds, and of liverwort extracts, on in vitro IAA-oxidase activity was also examined. 相似文献
2.
Marcelo Der Torossian Torres Adriana Farias Silva Flávio Lopes Alves Margareth Lara Capurro Antonio Miranda Vani Xavier Oliveira Junior 《International journal of peptide research and therapeutics》2014,20(3):277-287
Malaria is caused by the protozoa Plasmodium and is responsible for approximately one million deaths annually. The antimalarial effects of angiotensin II and its analogs against Plasmodium gallinaceum and falciparum have recently been reported. Here, 12 angiotensin II restricted analogs that contain i ? (i + 2), i ? (i + 3) and i ? (i + 4) lactam bridges were synthesized to analyze their effect on antiplasmodial activity. To accomplish this, peptides containing two amino acid residues (aspartic or glutamic acids and lysine or ornithine), were synthesized by the t-Boc solid phase method, purified by liquid chromatography, and characterized by mass spectrometry, and conformational studies were performed by circular dichroism. The results indicate that some of the analogs had anti-plasmodium activity similar to angiotensin II (88 % activity). Among those, eight compounds exhibited high activity (>70 %), measured by fluorescence microscopy. The analogs with smaller lactam rings and an aspartic acid residue as the bridgehead element had lower levels of lytic activity. The results obtained with the new restricted analogs showed that the insertion position (near the N-terminus), the ring size, and the number of residues between the rings are as important as the components of lactam bridge, regardless of their chirality. The circular dichroism studies suggest that the active analogs, and native angiotensin II, adopt a β-fold conformation in different solutions. In conclusion, this approach provides insight for understanding the effects of restricting the ring size and position on the bioactivity of angiotensin II and provides a new direction for the design of potential chemotherapeutic agents. 相似文献
3.
Intracellular Localization of Lunularic Acid and Prelunularic Acid in Suspension Cultured Cells of Marchantia polymorpha
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Intracellular localization of lunularic acid and prelunularic acid in suspension cultured cells of Marchantia polymorpha L. was studied. The sum of both compounds was determined as lunularic acid group (LNAs) because of the instability of prelunularic acid to convert into lunularic acid.
Mechanical disruption of the cells followed by differential centrifugation showed that LNAs was associated with the supernatant of 100,000g centrifugation. Protoplasts isolated from the cells were osmotically ruptured and the distribution of LNAs among the organelles was examined by discontinuous density gradient centrifugation of the protoplast contents. Successful isolation of intact chloroplasts, mitochondria and peroxisomes free from cytoplasm indicated that LNAs was not accumulated in these organelles. Flotation techniques resulted in an efficient isolation of pure vacuoles and revealed that LNAs was distributed almost equally in the vacuoles and cytoplasm.
相似文献4.
A ten-step synthesis of lunularic acid, starting from phenyl β-chloropropionate is described. Detailed spectroscopic data are given for lunularic 相似文献
5.
John Gorham 《Phytochemistry》1977,16(2):249-253
Lunularic acid and lunularin were detected in 76 species of hepatics, but not in any of the Anthrocerotales or Algae examined. Lunularic acid, lunularin, 3,4′-dihydroxystilbene and a glycoside of lunularic acid were also identified in extracts of Hydrangea macrophylla roots, together with hydrangenol, hydrangeic acid and their glucosides. 相似文献
6.
Prelunularic acid, the first example of an intermediate with a ‘pre-aromatic’ structure in the phenylpropanoid-polymalonate pathway, was isolated from suspension-cultured cells of Marchantia polymorpha. Its structure, including its absolute configuration, was assigned on the basis of spectral properties, direct conversion into lunularic acid, and CD measurements on the bis(p-dimethylaminobenzoate) of the methyl ester. Prelunularic acid was also detected in several liverworts of Marchantiales and Jungermanniales, and appears to be the immediate precursor of lunularic acid instead of the previously postulated hydrangenol or hydrangeic acid. 相似文献
7.
Some properties of a preparation of an enzyme, lunularic acid decarboxylase, from the liverwort Conocephalum conicum are described. The enzyme is normally bound and could be solubilized with Triton X-100; at least some of the bound decarboxylase activity appears to be associated with chloroplasts. For lunularic acid the enzyme has Km 8.7 × 10?5 M (pH 7.8 and 30°). Some substrate analogues have been tested but no other substrate was found. Pinosylvic acid is a competitive inhibitor for the enzyme, Ki 1.2 × 10?4 M (pH 7.8 and 30°). No product inhibition was observed. Lunularic acid decarboxylase activity has also been observed with a cell-free system from Lunularia cruciata. 相似文献
8.
R. J. Pryce 《Planta》1971,97(4):354-357
Summary By gas-liquid chromotography and thin layer chromatography, an endogenous growth inhibitor of Lunularia cruciata has been detected in seven other representatives of the class of liverworts. All liverworts so far examined have been found to contain lunularic acid. Evidence for the identity of the previously isolated, but unidentified, endogenous growth inhibitor of Marchantia polymorpha and lunularic acid is presented. 相似文献
9.
The amounts of lunularic acid in suspension cultured cells of Marchantia polymorpha and thallus of Conocephalum conicum were carefully re-exami 相似文献
10.
Ansari FL Wadood A Ullah A Iftikhar F Ul-Haq Z 《Journal of enzyme inhibition and medicinal chemistry》2009,24(1):151-156
In continuation of our previous study on the urease inhibition by a number of chalcones, 2,3-dihydro-1,5-benzothiazepines and 2,3,4,5-tetrahydro-1,5-benzothiazepines, FlexX docking has been exploited to get a deeper insight into the mechanism of their inhibitory action. A comparison of the IC(50) values of the active compounds reveals that, of the three classes of compounds studied, 2,3-dihydro-1,5-benzothiazepines were the most potent urease inhibitors. An in silico examination of these compounds showed that the activity is related to the interaction of ligand with the nickel metallocentre, its interaction with two amino acid residues, Asp224 and Cys322, in addition to the orientation of rings A and B in the catalytic core of the enzyme. The most active compound 2,3-dihydro-1,5-benzothiazepine (4) anchor tightly through a network of interactions with Ni701 and Ni702. This includes a number of hydrogen bonds and hydrophobic contacts with the amino acid residues in its vicinity. For their reduced analogs, the difference in the activity of different diastereomers has been observed to be configuration-dependent. This may be ascribed mainly to the difference in the orientation of ring B of the two stereoisomers and the extent of their interaction with Asp224 and Cys322 present in the catalytic core of the enzyme. 相似文献
11.
Farzana Latif Ansari Abdul Wadood Ahsan Ullah Fatima Iftikhar 《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):151-156
In continuation of our previous study on the urease inhibition by a number of chalcones, 2,3-dihydro-1,5-benzothiazepines and 2,3,4,5-tetrahydro-1,5-benzothiazepines, FlexX docking has been exploited to get a deeper insight into the mechanism of their inhibitory action. A comparison of the IC50 values of the active compounds reveals that, of the three classes of compounds studied, 2,3-dihydro-1,5-benzothiazepines were the most potent urease inhibitors. An in silico examination of these compounds showed that the activity is related to the interaction of ligand with the nickel metallocentre, its interaction with two amino acid residues, Asp224 and Cys322, in addition to the orientation of rings A and B in the catalytic core of the enzyme. The most active compound 2,3-dihydro-1,5-benzothiazepine (4) anchor tightly through a network of interactions with Ni701 and Ni702. This includes a number of hydrogen bonds and hydrophobic contacts with the amino acid residues in its vicinity. For their reduced analogs, the difference in the activity of different diastereomers has been observed to be configuration-dependent. This may be ascribed mainly to the difference in the orientation of ring B of the two stereoisomers and the extent of their interaction with Asp224 and Cys322 present in the catalytic core of the enzyme. 相似文献
12.
Ambo A Ohkatsu H Minamizawa M Watanabe H Sugawara S Nitta K Tsuda Y Okada Y Sasaki Y 《Bioorganic & medicinal chemistry letters》2012,22(6):2192-2194
To develop novel inhibitors of P-glycoprotein (P-gp), dimeric peptides related to an opioid peptide containing the Dmt-Tic pharmacophore were synthesized and their P-gp inhibitory activities were analyzed. Of the 30 analogs synthesized, N(α),N(ε)-[(CH(3))(2)Mle-Tic](2)Lys-NH(2) and its D-Lys analog were found to exhibit potent P-gp inhibitory activity, twice that of verapamil, in doxorubicin-resistant K562 cells. Structure-activity studies indicated that the correct hydrophobicity and spacer length between two aromatic rings are important structural elements in this series of analogs for inhibition of P-gp. 相似文献
13.
Stilbenecarboxylate biosynthesis: a new function in the family of chalcone synthase-related proteins
Eckermann C Schröder G Eckermann S Strack D Schmidt J Schneider B Schröder J 《Phytochemistry》2003,62(3):271-286
Chalcone (CHS), stilbene (STS) synthases, and related proteins are key enzymes in the biosynthesis of many secondary plant products. Precursor feeding studies and mechanistic rationalization suggest that stilbenecarboxylates might also be synthesized by plant type III polyketide synthases; however, the enzyme activity leading to retention of the carboxyl moiety in a stilbene backbone has not yet been demonstrated. Hydrangea macrophylla L. (Garden Hortensia) contains stilbenecarboxylates (hydrangeic acid and lunularic acid) that are derived from 4-coumaroyl and dihydro-4-coumaroyl starter residues, respectively. We used homology-based techniques to clone CHS-related sequences, and the enzyme functions were investigated with recombinant proteins. Sequences for two proteins were obtained. One was identified as CHS. The other shared 65-70% identity with CHSs and other family members. The purified recombinant protein had stilbenecarboxylate synthase (STCS) activity with dihydro-4-coumaroyl-CoA, but not with 4-coumaroyl-CoA or other substrates. We propose that the enzyme is involved in the biosynthesis of lunularic acid. It is the first example of a STS-type reaction that does not lose the terminal carboxyl group during the ring folding to the end product. Comparisons with CHS, STS, and a pyrone synthase showed that it is the only enzyme exerting a tight control over decarboxylation reactions. The protein contains unusual residues in positions highly conserved in other CHS-related proteins, and mutagenesis studies suggest that they are important for the structure or/and the catalytic activity. The formation of the natural products in vivo requires a reducing step, and we discuss the possibility that the absence of a reductase in the in vitro reactions may be responsible for the failure to obtain stilbenecarboxylates from substrates like 4-coumaroyl-CoA. 相似文献
14.
Ill-Young Lee Todd D. Gruber Amanda Samuels Minhan Yun Bora Nam Minseo Kang Kathryn Crowley Benjamin Winterroth Helena I. Boshoff Clifton E. Barry 《Bioorganic & medicinal chemistry》2013,21(1):114-126
A series of salicylanilides was synthesized based on a high-throughput screening hit against Mycobacterium tuberculosis. A free phenolic hydroxyl on the salicylic acid moeity is required for activity, and the structure–activity relationship of the aniline ring is largely driven by the presence of electron withdrawing groups. We synthesized 94 analogs exploring substitutions of both rings and the linker region in this series and we have identified multiple compounds with low micromolar potency. Unfortunately, cytotoxicity in a murine macrophage cell line trends with antimicrobial activity, suggesting a similar mechanism of action. We propose that salicylanilides function as proton shuttles that kill cells by destroying the cellular proton gradient, limiting their utility as potential therapeutics. 相似文献
15.
John Gorham 《Phytochemistry》1977,16(7):915-918
The presence, in Conocephalum conicum, of the enzymes phenylalanine ammonia lyase, trans-cinnamic acid-4-hydroxylase and lunularic acid decarbo 相似文献
16.
Treatment of experimental Pneumocystis carinii pneumonia with analogs of pentamidine 总被引:3,自引:0,他引:3
R R Tidwell S G Kilgore K A Ohemeng J D Geratz J E Hall 《The Journal of protozoology》1989,36(1):74S-76S
Seven analogs of pentamidine were tested for their activity against an immunosuppressed rat model of Pneumocystis carinii pneumonia. Structural alterations of the pentamidine molecule included variations of the alkyl chain linking the two p-amidino phenoxy moieties and relocation of the amidine groups from the para to the meta position on the phenoxy rings. All analogs of pentamidine were active against P. carinii pneumonia when compared to a saline-treated control group. One derivative, 1, 4-di(4'-amidinophenoxy)butane, proved to be statistically more active than the parent drug. 相似文献
17.
R.R. TIDWELL S. G. KILGORE K.A. OKEMENG J.D. GERATZ JAMES EDWIN HALL 《The Journal of eukaryotic microbiology》1989,36(1):74S-76S
Seven analogs of pentamidine were tested for their activity against an immunosuppressed rat model of Pneumocystis carinii pneumonia. Structural alterations of the pentamidine molecule included variations of the alkyl chain linking the two p-amidino phenoxy moieties and relocation of the amidine groups from the para to the meu position on the phenoxy rings. All analogs of pentamidine were active against P. carinii pneumonia when compared to a saline-treated control group. One derivative, 1, 4-di(4'-amidinophenoxy)butanc, proved to be statistically more active than the parent drug. 相似文献
18.
家蚕抑前胸腺肽类似物的活性鉴定和结构分析 总被引:2,自引:1,他引:1
以家蚕Bombyx mori抑前胸腺肽的氨基酸序列作为基础,通过氨基酸残基的添加、减少和置换,人工合成了一组与家蚕抑前胸腺肽结构类似的多肽。利用家蚕前胸腺体外培养技术,结合蜕皮激素放射免疫分析方法,鉴定了与抑前胸腺肽结构类似的多肽的生理活性,并对它们的活性特征、化学参数、结构和功能、信号传导途径进行了综合的比较和分析。类似物899808的生物学功能与抑前胸腺肽的相同而且活性近似;类似物899805和899809对家蚕前胸腺蜕皮激素的生物合成表现出随浓度增加而增加的促进作用,而低浓度下几乎不促进;899803、899804、899806和899807类似物对家蚕前胸腺蜕皮激素的生物合成的促进和抑制作用与它们的浓度有着依赖关系。实验结果表明,对抑前胸腺肽的氨基酸序列作任何改变,都导致其生理活性的下降、丧失甚至相反的活性。 相似文献
19.
C F Hassman J T Pelton S H Buck P Shea E F Heminger R J Broersma J M Berman 《Biochemical and biophysical research communications》1988,152(3):1070-1075
A series of six analogs of rat atrial natriuretic factor have been prepared by the solid-phase method. The modified analogs contain 8-amino octanoic acid (a simple alkyl spacer) in place of selected tripeptides. Binding affinity to cultured aortic smooth muscle cell membranes suggests that the sequence Arg11-Gly16 is important for binding. Vasorelaxant activity on serotonin contracted rabbit aortic rings indicates that the Phe8-Gly16 sequence must be present for vasorelaxation. In anesthetized rats, the natriuretic and diuretic effects of an IV bolus dose correlate with in vitro vasodilatory activity. The alkyl spacer approach provides a facile method to quickly determine key regions of a large peptide involved in molecular recognition. 相似文献
20.
Comparison of the effects of vitamin A and its analogs upon rabbit ear cartilage in organ culture and upon growth of the vitamin A-deficient rat 总被引:1,自引:0,他引:1
A study was conducted to explore the relationship between the effects of vitamin A upon cartilage and the biological role of vitamin A in maintaining growth and life. Retinol, retinoic acid, alpha-retinoic acid, and ROB-7699 (a cyclopentyl analog of retinoic acid) were highly effective in promoting the lysis of the extracellular matrix of cartilage grown in organ culture in vitro. Retinoic acid and its two analogs were quantitatively more active than was retinol in bringing about lysis of matrix and release of proteoglycan into the culture medium. A bioassay was then conducted to determine the ability of each compound to promote growth of vitamin A-deficient rats. In contrast to their effects upon cartilage, retinoic acid and its two analogs were considerably less active quantitatively than retinol in promoting growth of vitamin A-deficient rats. Moreover, the three acids tested showed graded biological activity in the growth bioassay, with alpha-retinoic acid showing reduced bioactivity (approx. one-fourth that of retinoic acid) and ROB-7699 being virtually inactive. The lysis of cartilage produced by these compounds was presumably caused by release of lysosomal enzymes as a result of the membrane-labilizing effects of the compounds. Thus, these membrane effects of the vitamin A-related compounds are poorly correlated with their biological growth-promoting activity. The alpha-ionone analogs of retinol and retinoic acid were able to maintain good health and growth of vitamin A-deficient rats, although their quantitative activity was low. Rats fed alpha-retinyl acetate showed high liver stores of alpha-retinyl esters and low levels of serum retinol-binding protein (similar to the levels seen in retinoic acid-fed rats). The biological activity of the alpha-ionone analogs was apparently not due to contamination with or conversion to the normal beta-ionone compounds. 相似文献