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1.
Koichiro Shimomura Hiroshi Sudo Hitoshi Saga Hiroshi Kamada 《Plant cell reports》1991,10(6-7):282-285
Summary Hairy root cultures of Lithospermum erythrorhizon were established by transformation of in vitro grown shoots with Agrobacterium rhizogenes 15834. Hairy roots cultured on Murashige and Skoog solid medium did not produce any red pigments. However, the hairy roots cultured in Root Culture solid or liquid media produced a large amount of red pigments, which were released to the medium. The addition of adsorbents to the culture medium stimulated shikonin production by ca. 3-fold. Using this method an air-lift fermenter system was established, equipped with a XAD-2 column, which continuously produced ca. 5 mg/day of shikonin during a period of more than 220 days. 相似文献
2.
Kaori Touno Jin Tamaoka Yuko Ohashi Koichiro Shimomura 《Plant Physiology and Biochemistry》2005,43(2):101-105
Lithospermum erythrorhizon shoots, cultured on phytohormone-free Murashige and Skoog solid medium, produced shikonin derivatives, whereas shoots cultured in well-ventilated petri dishes, produced small amount. Analysis by gas chromatography revealed the presence of ethylene in non-ventilated petri dishes where the shoots, producing shikonin derivatives, were cultured. Therefore, the possible involvement of ethylene in shikonin biosynthesis of shoot cultures was investigated. Treatment of ethylene or the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, resulted in increasing shikonin derivatives contents in cultured shoots. Silver ion, an ethylene-response inhibitor, or aminoethoxyvinylglycine, an ethylene biosynthesis inhibitor, decreased production of shikonin derivatives in cultured shoots. Our results indicate that ethylene is one of the regulatory elements of shikonin biosynthesis in L. erythrorhizon shoot culture. 相似文献
3.
Khadijeh Zare Hossein Nazemiyeh Ali Movafeghi Mahmood Khosrowshahli Alireza Motallebi-Azar Mohammadreza Dadpour Yadollah Omidi 《Plant Cell, Tissue and Organ Culture》2010,100(2):157-164
An in vitro cell suspension culture of Echium italicum was established and assayed for the production of shikonin and alkannin derivatives. Callus tissues were induced from cotyledon
explants of the plant incubated onto the solidified B5 medium. A two-liquid-phase system suspension culture was then established
to elicit pigments of shikonin and alkannin derivatives using liquid paraffin. The presence of liquid paraffin efficiently
induced production of pigments in cultured cells. The production and/or accumulation of these compounds in the E. italicum cells was examined using fluorescence microscopy as the naphthoquinone molecules display autofluorescent properties. Phytochemical
analysis of the n-hexane extract of the medium was also carried out using preparative HPLC. The chemical structure of shikonin and alkannin
derivatives were characterized by UV, 1H-NMR, and 13C-NMR techniques. Based on our findings, this bioprocess engineering approach
resulted in induction of shikonin and alkannin derivatives, whereupon it may be recruited for production of these important
secondary metabolites. 相似文献
4.
Cotyledons of various ages from seedlings of eight watermelon (Citrullus vulgaris) cultivars were cultured on MS medium supplemented with different combinations of phytohormones. High frequency shoot regeneration (60.0–92.0%) was induced from 5-day-old cotyledons of cultivars cultured on MS medium containing 5.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l indole-3-acetic acid (IAA). Multiple shoot buds elongated on MS medium containing 0.2 mg/l kinetin (KT) and 5–10 shoots per expiant could be recovered depending on the cultivars. Elongated shoots rooted on MS medium with 0.1 mg/l -naphthalene acetic acid (NAA). Zeatin riboside (ZT) had a similar efficiency as BA in shoot induction, and it was significantly more functional than 2-isopentenyladenine (2iP) or kinetin (KT). Cotyledons from 5-day-old seedlings were the most responsive to shoot induction.Abbreviation BA
6-benzylaminopurine
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- 2iP
2-isopentenyladenine
- KT
kinetin
- MS
Murashige and Skoog (1962)
- NAA
-naphthalene acetic acid
- ZT
zeatin riboside 相似文献
5.
Kayo Ideda Daisuke Teshima Toshinobu Aoyama Motoyoshi Satake Koichiro Shimomura 《Plant cell reports》1988,7(4):288-291
Shoot cultures of Cephaelis ipecacuanha A. Richard were established by using shoot tips as initial explants. Multiple shoots were obtained from node segments upon culture on B5 medium supplemented with NAA-BA (0.01–3, 5 mg/l). These shoots were rooted on B5 and 1/2 MS media containing IAA or NAA, and the regenerated plants were transferred to soil and grown in a greenhouse. The emetic alkaloids of the regenerated plants, mother plants and leaves of shoot cultures were analyzed by TLC and HPLC. Seven months of growth under greenhouse condition, the contents of the emetic alkaloids in the regenerated plants were comparable to those of the mother plants.Abbreviations B5
Gamborg B5 (1968) medium
- MS
Murashige-Skoog (1962) medium
- 1/2 MS
a half strength MS medium
- NAA
1-naphthaleneacetic acid
- IAA
indole-3-acetic acid
- Kin
kinetin
- BA
6-benzylaminopurine
- TLC
thin layer chromatography
- HPLC
high performance liquid chromatography 相似文献
6.
Daisuke Teshima Kayo Ikeda Motoyoshi Satake Toshinobu Aoyama Koichiro Shimomura 《Plant cell reports》1988,7(4):278-280
Callus and adventitious roots were induced on leaf segments from shoot culture of Cephaelis ipecacuanha A. Richard on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, 1-naphthaleneacetic acid and kinetin. The contents of emetic alkaloids in calli, roots and root suspension cultures were quantified by HPLC. Roots cultured in solid and liquid Murashige-Skoog media yielded emetine and cephaeline. The amount of the two alkaloids in the root suspension culture was very similar to that of roots from ipecac mother plant grown in a greenhouse. In contrast, calli subcultured on Murashige-Skoog media containing combinations of 2,4-dichlorophenoxyacetic acid and kinetin produced only trace amounts of emetic alkaloids.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- NAA
l-naphthaleneacetic acid
- Kin
kinetin
- MS
Murashige-Skoog
- EM
emetine
- CP
cephaeline
- DW
dry weight. 相似文献
7.
Plant regeneration of Actinidia deliciosa var. deliciosa cv. Hayword was obtained from protoplasts isolated from petiole derived long-term callus cultures. Protoplasts were cultured in liquid medium over agarose gelled medium. Regenerated green callus, plated on solid medium, could develop shoots that rooted spontaneously in hormone-less medium. The plants obtained are growing fast in soil and present a normal phenotype.Abbreviations BAP
benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- DTT
dithiotreitol
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- Kin
kinetin
- MES
2-(N-morpholino) ethanesulphonic acid
- MS
Murashige and Skoog (1962) medium
- NAA
naphthalene-1-acetic acid
- SH
Schenk and Hildebrandt (1972) medium
This Research was supported by JNICT and INIC 相似文献
8.
Summary Callus derived from Symphytum officinale L. regenerants was cultured in the presence of various phytohormones. The growth rate of callus was stimulated by all phytohormones at various concentrations. With 1-naphthaleneacetic acid no organ differentiation could be observed. With indole-3-butyric acid at low concentrations only roots were formed, whereas 6-benzylaminopurine, kinetin and zeatin at various concentrations induced either root or shoot formation or the simultaneous regeneration of both. Minor amounts of fructans were formed at high 6-benzylaminopurine-, zeatin- and at all indole-3-acetic acid-concentrations. The concentration of 1-naphthaleneacetic acid had no influence on the fructan content. Highest rates of fructan synthesis occurred at low zeatin-concentrations up to 1.5 mg/l. Only zeatin at all concentrations induced the synthesis of polyfructans, whereas appreciable amounts of oligofructans were formed under the influence of all other phytohormones.Abbreviations NAA
1-naphthaleneacetic acid
- IBA
indole-3-butyric acid
- BAP
6-benzylaminopurine
- IAA
indole-3-acetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- DP
degree of polymerisation
- GLC
gas liquid chromatography
- TLC
thin layer chromatography 相似文献
9.
Cao Dinh Hung Krystyna Johnson Fraser Torpy 《In vitro cellular & developmental biology. Plant》2006,42(6):548-552
Summary An efficient protocol for in vitro propagation of the valuable medicinal plant, Wasabia japonica (Miq.) Matsumura is described through shoot tip proliferation and direct regeneration. Multiple shoots were induced from
shoort tips cultured on Murashige and Skoog (MS) semi-solid medium containing various concentrations (0.5–50 μM) of N6-benzyladenine (BA), thidiazuron, kinetin, and zeatin. A comparison was made on shoot multiplication between semi-solid and
liquid culture media. Well-developed shoots were obtained using full-strength MS semi-solid medium containing 5.0 μM BA. However, the greatest shoot proliferation was achieved on either full- or half-strength MS liquid media supplemented
with 5.0 μM BA for 4 wk (15.3±0.9 and 15.0±0.7 shoots per explant, respectively), and on half-strength MS liquid medium for 6 wk (25.8±1.3
shoots per explant) in culture. In contrast, the maximum number of shoots per explant on full-strength MS semi-solid medium
was achieved with either 5.0 μM BA (10.4±0.6 shoots per explant) or 10.0 μM kinetin (10.9±0.8 shoots per explant). Fresh weight of explants and length of shoots derived from full-strength MS liquid
medium (1055±77 mg and 34.2±1.0 mm, respectively) were significantly higher than those derived from full-strength MS semisolid
medium (437.6±17.3 mg and 15.4±0.7 mm, respectively). Quarter-strength MS liquid medium had no significant difference in shoot
proliferation when compared to quarter-strength MS semi-solid medium. Elongated shoots were separated and rooted on half-strength
MS semi-solid media fortified with 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), or indole-3-acetic acid (IAA)
ranging from 0.1 to 10.0 μM. Root formation was greatest with IBA when compared with IAA and NAA. One hundred percent of shoots were rooted on half-strength
MS medium with 5.0 μM IBA, while vigorous roots were obtained with 10.0 μM IBA. Micropropagated plantlets were successfully established in soil with 95% survival rate after heardening. 相似文献
10.
Filipendula ulmaria (L.) Maxim (meadowsweet) is a medicinal plant that is claimed to have several biological activities, including anti-tumor,
anti-carcinogenic, anti-oxidant, anti-coagulant, anti-ulcerogenic, anti-microbial, anti-arthritic, and immunomodulatory properties.
This report describes, for the first time, an efficient plant regeneration system for F. ulmaria via adventitious shoot development from leaf, petiole, and root explants cultured on Murashige and Skoog’s minimal organics
medium containing different concentrations of thidiazuron (TDZ), benzyladenine, and kinetin either alone or in combination
with different auxins. Relatively extensive/prolific shoot regeneration was observed in all three explant types with TDZ in
combination with indole-3-acetic acid (IAA). Gibberellic acid (GA3), TDZ, and IAA combinations were also tested. The best shoot proliferation was observed among root explants cultured on media
supplemented with 0.45 μM TDZ + 2.85 μM IAA + 1.44 μM GA3. Regenerated shoots were transferred to rooting media containing different concentrations of either IAA, indole-3-butyric
acid (IBA), naphthalene acetic acid, or 2,4-dichlorophenoxyacetic acid. Most shoots developed roots on medium with 2.46 μM
IBA. Rooted explants were transferred to vermiculite in Magenta containers for a 2-wk acclimatization period and then finally
to plastic pots containing potting soil. The plantlets in soil were kept in growth chambers for 2 wk before transferring to
greenhouse conditions. 相似文献
11.
V. A. Chauhan P. C. Josekutty Y. T. Jasrai G. Prathapasenan 《Journal of plant biochemistry and biotechnology.》1996,5(2):117-118
Micropropagation of Dalbergia sissoo Roxb was achieved through in vitro proliferation of axillary buds from 30 to 40 years old mature tree. Bud-break was achieved within six days when nodal explants were cultured on MS medium supplemented with kinetin (9.2 μm), indole-3-butyric acid (2.46 μM) apd 6-benzyladenine (13.2 μM). Multiple shoot formation occurred from nodal explants of in vitro raised shoots on MS medium with reduced levels of major salts and kinetin. Roots were Induced within 5 days on in vitro generated shoots on MS medium supplemented with 1-naphthalene acetic acid (0.53 μM) and indole-3-butyric acid (9.8 μM). 相似文献
12.
根癌农杆菌转化紫草的研究 总被引:7,自引:0,他引:7
紫草 (LithospermumerythrorhizonSieb .etZucc)是传统中药。其根部含有萘醌类化合物—紫草素及其衍生物 ,具有显著的抗菌、抗炎、抗癌以及促进伤口愈合等生理活性。紫草素同时也是一种名贵化妆品染料。科学家对紫草的研究兴趣是基于其资源的缺乏及紫草植物本身所具有的一些特点 ;如 :紫草素及其衍生物的颜色特性可凭借肉眼观察 ,紫草素及其衍生物只在紫草的根部积累 ,紫草素合成的次生代谢途径受多种酶和外界条件 (光照 ,营养等 )的调节等。紫草细胞培养 (Fujita等 ,1983;叶和春等 ,1991)可以产… 相似文献
13.
Hee-Ju Yu Soo Kyung Oh Man-Ho Oh Dong-Woog Choi Young Myung Kwon Sang-Gu Kim 《Plant cell reports》1997,16(5):261-266
We previously studied the production of shikonin derivatives by cell lines ofLithospermum erythrorhizon. As a result, we have obtained a cell line LE 87, which exhibited high cell growth and high shikonin production. In the present study, the effects of auxins (2,4-D, IAA, picloram, and NAA) and cytokinins (BAP and kinetin) on organogenesis and somatic embryogenesis in this shikonin-producing cell line were investigated. The highest organogenic and embryogenic efficiency was obtained on MS medium supplemented with 10 µM NAA and 0.3 µM kinetin. Subcultured calli showed different morphogenic frequencies depending on the NAA and kinetin concentration. Morphologically normal plants have been regenerated via mostly organogenesis. Shoots subsequently produced roots on plant growth regulator-free MS medium and developed into plantlets. In most cases, a few thin roots were formed at the bases of the shoots after four weeks on the rooting medium. More than fifty green plantlets were transplanted to soil in pots and developed into phenotypically normal plants 8 weeks after being transferred to soil. The regenerated plants grew to maturity, flowered, and set seeds by only artificial pollination.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- NAA
-naphthalene-acetic acid
- MS
Murashige and Skoog (1962) medium
Communicated by S. Gleddie 相似文献
14.
Yaowapha Jirakiattikul Panumart Rithichai Thipsukon Boonyeun Srisopa Ruangnoo Arunporn Itharat 《Physiology and Molecular Biology of Plants》2020,26(3):585-591
Dioscorealide B is an important secondary metabolite isolated from Dioscorea membranacea Pierre ex Prain & Burkill. The effect on secondary metabolite content of different concentrations of two elicitors [jasmonic acid (JA) and salicylic acid (SA)], and of medium status and JA exposure period were investigated. In the JA and SA concentration experiment, 6-week-old shoots were cultured on MS medium supplemented with 8.87 µM BA (6-benzyladenine) in combination with 100–500 µM JA or 50–200 µM SA for 3 weeks. MS medium supplemented only with 8.87 µM BA was used as a control. The highest dioscorealide B content was recorded in the 100 µM JA shoots. To determine the optimal medium status and JA exposure period, shoots were cultured on solid and in liquid MS media supplemented with 8.87 µM BA and 100 µM JA for 2, 3, 4 and 5 weeks. No interaction was found between the medium status and the elicitor exposure period in the dioscorealide B production. Shoots cultured on the solid MS medium supplemented with 100 µM JA had a higher dioscorealide B content (0.57 ± 0.35% w/w) than those cultured in liquid medium (0.36 ± 0.40% w/w) and 5-week JA exposure produced the highest dioscorealide B content of 1.05 ± 0.15% (w/w). 相似文献
15.
Soma Saha Madhumita J. Mukhopadhyay Sandip Mukhopadhyay 《Journal of plant biochemistry and biotechnology.》2003,12(1):61-64
The present study involves in vitro propagation of Hemidesmus indicus (L) R Br through bud multiplication and subsequent plant regeneration. The buds multiplied to produce numerous shoots at variable rates in presence of a-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) as well as NAA and kinetin. The best response in bud multiplication was obtained in Murashige and Skoog’s (MS) basal medium supplemented with 0.1 mg I-1 NAA and 2.0 mg I-1 BAP (7-8 shoots per explant) and the bud break time was only 4 days after inoculation. The multiplication rate was low when the buds were cultured in NAA and kinetin media and the shootlets regenerated were very thin, weak and elongated. The shoots regenerated were further cultured on MS and half strength MS basal media with variable levels of indole-3-butyric acid (IBA) for initiation of roots. Culture of shootlets for 34 weeks in one half strength of MS medium followed by culturing in the same medium with 1.5 mg 1-1 IBA induced highest production of roots (3-5 roots per shoot) within 2 weeks. Chromosome number stability with no detectable structural changes was observed in the regenerates. The rooted plants were successfully established in the soil with 85% survival rate. 相似文献
16.
Mészáros Annamária Bellon Andrea Pintér Éva Horváth Gábor 《Plant Cell, Tissue and Organ Culture》1999,57(2):149-152
Traditional propagation of lemon balm (Melissa officinalis L.) is inefficient for establishing a good quality clonal population.
Results of the presented experiments outline an effective method for micropropagation of this species. Following culture initiation
from shoots of field-grown plants on growth regulator free Murashige–Skoog medium, rapid shoot multiplication with only rudimentary
root formation could be achieved on media containing various concentrations of indole-3-acetic acid and 6-benzyladenine. The
combination of 5.71 μM indole-3-acetic acid and 6.66 μM 6-benzyladenine resulted in the best multiplication. Transfer of propagules
to media containing indole-3-acetic acid and kinetin did not result in shoot proliferation; however, single plantlets grown
on media containing 5.71 μM indole-3-acetic acid and 13.9 μM kinetin developed more compact shoots and stronger roots than
the control plants and were suitable for acclimatisation with an efficiency over 95%.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
17.
An efficient and simple method for plant regeneration from immature lentil seeds (Lens culinaris) is described. Immature seeds from 1 to 6 mm of four lentil cultivars were cultured in vitro on 10 different media. Culture media included different concentrations of N
6
-benzylaminopurine (BAP), alone or in combination with other phytohormones. After 4 weeks in culture, multiple shoot regeneration
was observed using media with BAP. Immature seed size showed significant effect on shoot regeneration. Regenerated shoots
(up to 4 shoots per explant on medium with Kinetin (KN) and from 5 to 20 on media with BAP) formed adventitious roots 30 days
after transferring them to a medium containing 11.4 μM indole-3-acetic acid (IAA). The efficiency of the rooting medium varied
depending upon the shoot-regeneration medium and the cultivar tested. The highest rooting percentage (88.9%) was obtained
from regenerated shoots of the cultivar Verdina on a medium with 1 μM α-naphthaleneacetic acid (NAA).
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
18.
Protoplasts isolated from both 7-day-old light-grown and 4-day-old dark/dim light-grown cotyledons of four Brassica campestris varieties (Arlo, Sonja, Bunyip and Wonk Bok) were cultured in three liquid media: modified K8P, modified MS and modified Pelletier's B to compare the capacities for cell division and plant regeneration. Following cell wall regeneration the cultured protoplasts from dark/dim light-grown cotyledons of four varieties showed rapid division and high frequency of cell division compared with those isolated from light-grown cotyledons. The frequencies of cell division were significantly influenced by varieties and culture media but only in cultured protoplasts isolated from dark/dim light-grown cotyledons. The interaction between varieties and media was also significant. Cell colonies formed within 7–14 days in protoplast cultures from dark/dim light-grown cotyledons, and calli subsequently grown on a solid medium developed shoots when transferred onto a regeneration medium. Three of four tested varieties (Arlo, Sonja and Bunyip) showed shoot regeneration within 2–3 months after protoplast isolation, with a high degree of reproducibility in Arlo and Bunyip. Regenerated shoots, which were induced to root on half-strength MS medium with 0.1 mg.l–1 IBA, survived in soil and grew to produce siliques and set viable seeds in the greenhouse. The present report is the first to document the production of regenerated plants that set seeds in Brassica campestris from cotyledonary protoplasts.Abbreviations BAP
benzylaminopurine
- CPW
Composition of Protoplast Washing-solution
- 2,4-D
2,4-dichlorophenoxyacetic acid
- EDTA
ethylenediamine-tetraacetic acid
- GA3
gibberellic acid
- IBA
indole-3-butyric acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog medium
- NAA
-naphthaleneacetic acid
- KT
kinetin
- FDA
fluorescein diacetate
- SDS
sodium dodecyl sulfate 相似文献
19.
In vitro regeneration of plantlets and multiplication of Sesbania bispinosa (Jacq.) W.F. Wight plants from cultured callus tissue were demonstrated. Callus was established from both cotyledons and mature leaflets on Murashige and Skoog (MS) basal medium supplemented with BAP (0.5 mg/l) and 2,4-D (2 mg/l). Callus mediated shoot bud differentiation was studied under defined nutritional, hormonal and cultural conditions. Various concentrations of BAP or kinetin (Kn) with coconut milk (CM) in MS media induced different levels of shoot bud differentiation as well as multiplication. Multiple shoot bud differentiation occurred in most of the primary calli. The best medium for shoot bud differentiation from cotyledon derived callus, contained BAP (2 mg/l) and 15% CM (V/V). More efficient shoot bud organogenesis was recorded with BAP than Kn. Supplementation with CM in MS media accelerated shoot bud organogenesis in differentiating callus tissue. Rooting of differentiated shoots was achieved by a three step culture procedure involving (a) MS solid medium containing IBA (2 mg/l), (b) growth regulator free half strength MS medium with 1% charcoal, and (c) half strength MS liquid medium free of vitamins, growth regulators and charcoal.Abbreviations IAA
indoleacetic acid
- IBA
indole-3-butyric acid
- NAA
naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- BAP
6-benzylaminopurine
- Kn
kinetin
- CM
coconut milk
- MS
Murashige and Skoog's medium
- SBI
shoot bud inducing medium 相似文献
20.
Mature embryos of Costus speciosus were excised and cultured on Schenk and Hildebrandt's (1972) nutrient medium containing auxins and cytokinins either alone or in combination. Multiple shoots were obtained when kinetin and indole-3-butyric acid were supplemented each at 0.1 mg 1–1 concentration. Embryo-derived plantlets were multiplied through propagation of rhizomes and the propagules derived from a single embryo were designated as an embryoclone. Twenty such embryo-clones were maintained in the field. Variations in rhizome biomass yield and diosgenin contents of these embryoclones were noted. Thirty-six percent of the embryo-clones studied were high diosgenin yielding types. Diosgenin contents at the intraclonal level were uniform. The in vitro raised plants were morphologically uniform and indistinguishable from their parent.Abbreviations SH
Schenk and Hildebrandt (1972) medium
- Kn
kinetin
- BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- IBA
indole-3-butyric acid
- IAA
indole-3-acetic acid
- CA
casaminoacids (vitamin free)
- TLC
thin layer chromatography 相似文献