共查询到20条相似文献,搜索用时 15 毫秒
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Feng Han Kazuhisa Takeda Masao Ono Satoru Yokoyama Yotaro Shinozawa Shigeki Shibahara 《Biochemical and biophysical research communications》2009,385(3):449-676
Hypoxemia is a common manifestation of various disorders and generates pressure overload to the heart. Here we analyzed the expression of lipocalin-type prostaglandin D synthase (L-PGDS) in the heart of C57BL/6 mice kept under normobaric hypoxia (10% O2) that generates hemodynamic stress. Northern and Western blot analyses revealed that the expression levels of L-PGDS mRNA and protein were significantly increased (>twofold) after 14 days of hypoxia, compared to the mice kept under normoxia. Immunohistochemical analysis indicated that L-PGDS was increased in the myocardium of auricles and ventricles and the pulmonary venous myocardium at 28 days of hypoxia. Moreover, using C57BL/6 mice lacking heme oxygenase-2 (HO-2−/−), a model of chronic hypoxemia, we showed that the expression level of L-PGDS protein was twofold higher in the heart than that of wild-type mouse. L-PGDS expression is induced in the myocardium under hypoxemia, which may reflect the adaptation to the hemodynamic stress. 相似文献
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Matthews J Wihlén B Tujague M Wan J Ström A Gustafsson JA 《Molecular endocrinology (Baltimore, Md.)》2006,20(3):534-543
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L Zhou M T Wang C B Lawrence S M Chang D L Steffen 《Molecular endocrinology (Baltimore, Md.)》1989,3(7):1157-1164
A high titer retroviral vector containing the cDNA of human estrogen receptor (hER) was generated and used to transfer the hER gene into the rat 208F cell line. Southern blot analysis showed the integration of the provirus to be at a unique site and that the provirus was intact in the genome of recipient cells. The expression of the integrated hER gene in the infected rat cells was detected by Northern blot analysis and by a functional assay in which the hER gene product stimulated the production of a chloramphenicol acetyl transferase gene under the control of an estrogen-responsive element. These experiments demonstrate the feasibility of using a retroviral vector system to introduce a functional ER gene into cultured cells lacking this receptor. 相似文献
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Synergism of closely adjacent estrogen-responsive elements increases their regulatory potential 总被引:23,自引:0,他引:23
Recent gene transfer experiments have shown that an estrogen-responsive DNA element (ERE) GGTCANNNTGACC mediates the estrogen inducibility of the Xenopus laevis vitellogenin A1 and A2 genes as well as the chicken vitellogenin II gene. We report here on experiments that explain the estrogen regulation of the Xenopus vitellogenin B1 and B2 genes. In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility. Furthermore we show that synergism of EREs is most efficient, when the two elements are closely adjacent and that it is lost when the synergistic elements are separated by 125 basepairs. In-vitro estrogen receptor binding experiments indicate that co-operative binding of estrogen receptors to closely adjacent EREs is not essential for synergism of ERE homologues that have no intrinsic regulatory capacity. Functional synergism of EREs is observed in the human estrogen-responsive MCF-7 cell line as well as in mouse fibroblasts (Ltk-) cotransfected with estrogen receptor expression vectors. Even expression of a truncated receptor protein lacking 178 amino acid residues of the amino-terminal end allows synergism, suggesting that the amino-terminal end preceding the DNA-binding domain of the estrogen receptor is not required. 相似文献
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Liu MM Albanese C Anderson CM Hilty K Webb P Uht RM Price RH Pestell RG Kushner PJ 《The Journal of biological chemistry》2002,277(27):24353-24360
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Eugene A. Berkowitz Marilyn I. Evans 《In vitro cellular & developmental biology. Animal》1992,28(6):391-396
Summary The estrogen-responsive Leghorn strain M chicken hepatoma (LMH) cell line provides a model system for studying the estrogen-dependent,
liver-specific expression of avian genes. Serum-free culture conditions have been established that allow expression of apolipoprotein
B, very low density apolipoprotein II (apoVLDLII), serum albumin, and transferrin at levels detectable by Northern blot analysis.
Regulation of apoVLDLII mRNA by estrogen occurred in an appropriate time-and dose-dependent manner in serum-free cultures
of the LMH cells. The expression of apoVLDLII mRNA in serum-free culture was at least 100-fold higher than that expressed
in cultures containing 10% serum. The level of estrogen receptors in LMH cells cultured with 10% serum was approximately 2000
receptors per cell, and in serum-free culture approximately 1000 receptors per cell. When these cells were transfected with
estrogen receptor DNA and cultured in serum-free medium, apoVLDLII mRNA was decreased relative to that expressed in cells
transfected with a control plasmid. These results indicate that when the LMH cells are cultured without serum, estrogen receptors
are not the limiting factor for the expression of the apoVLDLII gene. 相似文献