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1.
Taizo Motomura Chikako Nagasato Yoshibumi Komeda Kazuo Okuda 《Journal of phycology》2001,37(5):783-792
Mitosis in Boergesenia forbesii (Harvey) Feldman was studied by immunofluorescence microscopy using anti-β–tubulin, anti-γ–tubulin, and anti-centrin antibodies. In the interphase nucleus, one, two, or rarely three anti-centrin staining spots were located around the nucleus, indicating the existence of centrioles. Microtubules (MTs) elongated randomly from the circumference of the nuclear envelope, but distinct microtubule organizing centers could not be observed. In prophase, MTs located around the interphase nuclei became fragmented and eventually disappeared. Instead, numerous MTs elongated along the nuclear envelope from the discrete anti-centrin staining spots. Anti-centrin staining spots duplicated and migrated to the two mitotic poles. γ–Tubulin was not detected at the centrioles during interphase but began to localize there from prophase onward. The mitotic spindle in B. forbesii was a typical closed type, the nuclear envelope remaining intact during nuclear division. From late prophase, accompanying the chromosome condensation, spindle MTs could be observed within the nuclear envelope. A bipolar mitotic spindle was formed at metaphase, when the most intense staining of γ-tubulin around the centrioles could also be seen. Both spindle MT poles were formed inside the nuclear envelope, independent of the position of the centrioles outside. In early anaphase, MTs between separating daughter chromosomes were not detected. Afterward, characteristic interzonal spindle MTs developed and separated both sets of the daughter chromosomes. From late anaphase to telophase, γ-tubulin could not be detected around the centrioles and MT radiation from the centrioles became diminished at both poles. γ-Tubulin was not detected at the ends of the interzonal spindle fibers. When MTs were depolymerized with amiprophos methyl during mitosis, γ-tubulin localization around the centrioles was clearly confirmed. Moreover, an influx of tubulin molecules into the nucleus for the mitotic spindle occurred at chromosome condensation in mitosis. 相似文献
2.
Martha J. Powell 《American journal of botany》1980,67(6):839-853
The structure of centric, intranuclear mitosis and of organelles associated with nuclei are described in developing zoosporangia of the chytrid Rhizophydium spherotheca. Frequently dictyosomes partially encompass the sides of diplosomes (paired centrioles). A single, incomplete layer of endoplasmic reticulum with tubular connections to the nuclear envelope is found around dividing nuclei. The nuclear envelope remains intact during mitosis except for polar fenestrae which appear during spindle incursion. During prophase, when diplosomes first define the nuclear poles, secondary centrioles occur adjacent and at right angles to the sides of primary centrioles. By late metaphase the centrioles in a diplosome are positioned at a 40° angle to each other and are joined by an electron-dense band; by telophase the centrioles lie almost parallel to each other. Astral microtubules radiate into the cytoplasm from centrioles during interphase, but by metaphase few cytoplasmic microtubules are found. Cytoplasmic microtubules increase during late anaphase and telophase as spindle microtubules gradually disappear. The mitotic spindle, which contains chromosomal and interzonal microtubules, converges at the base of the primary centriole. Throughout mitosis the semipersistent nucleolus is adjacent to the nuclear envelope and remains in the interzonal region of the nucleus as chromosomes separate and the nucleus elongates. During telophase the nuclear envelope constricts around the chromosomal mass, and the daughter nuclei separate from each end of the interzonal region of the nucleus. The envelope of the interzonal region is relatively intact and encircles the nucleolus, but later the membranes of the interzonal region scatter and the nucleolus disperses. The structure of the mitotic apparatus is similar to that of the chytrid Phlyctochytrium irregulare. 相似文献
3.
We have used anti-tubulin antibodies and immunofluorescence microscopy to determine the overall distribution of microtubules during interphase and mitosis in both the myxamoebae and plasmodia of the slime mold Physarum polycephalum. We have paralleled these observations with electron microscopy of the same stages. The myxamoebae possess a network of cytoplasmic microtubules whilst the coenocytic plasmodium does not possess any cytoplasmic microtubules--at either interphase or mitosis. In plasmodia microtubules are, however, elaborated by an intranuclear microtubule organizing centre (MTOC) during prophase of mitosis and these microtubules proceed to form part of the mitotic spindle. There is little difference in the overall distribution and arrangement of microtubules during division of either the myxamoebal or plasmodial nuclei. These findings are discussed in relation to the synthesis of tubulin during the plasmodial cell cycle and the rearrangements of the nuclear envelope during mitosis. 相似文献
4.
Summary Myxamoebae ofEchinostelium minutum exhibit extranuclear (open spindle) mitosis with centrioles present at the poles. Spindle microtubules are formed in association with a juxtanuclear MTOC which surrounds the cell's complement of centrioles. During late prophase or prometaphase the nuclear envelope breaks down and subsequently a metaphase plate is formed. Two anaphasic movements occur sequentially: firstly, the distance of the chromosomes to the poles shortens; secondly the distance between the spindle poles increases. The arrangement of spindle microtubules during anaphase is consistent with the hypothesis that chromosomal separation is due to lateral interaction (zippering) of microtubules. During telophase, reconstitution of the nuclear envelope usually takes place in the interzonal region prior to reformation in the polar region. Cytokinesis, which begins in anaphase or early telophase involves the participation of vesicles, microfilaments and microtubules.Based on the doctoral dissertation of the first author presented to the Department of Botany, University of Washington, Seattle, WA 98195, U.S.A. 相似文献
5.
Frans A. C. Kouwets 《Protoplasma》1996,191(3-4):191-204
Summary The ultrastructure of mitosis and cytokinesis is studied in the typical and a multicentriolar form of the multinucleate green algaBracteacoccus minor (Chodat) Petrovà. These processes are essentially identical in both forms, and are similar to those in other uni- and multinucleate chlorellalean algae. The mitotic spindle is closed and centric, and a fragmentary perinuclear envelope is present. In multinuclear cells mitosis is synchronous and may occur at the same time as cytokinesis. Cleavage is simultaneous and centrifugal, starting near the nucleus-associated centrioles and apparently mediated by phycoplast microtubules of the trochoplast type. Flagellated wall-less spores are usually formed. In the typical form ofB. minor, each interphase nucleus is associated with two mature centrioles (= one set) which function as centrosomal markers. At the onset of mitosis these centrioles duplicate and segregate and eventually establish the two poles of the spindle, where polar fenestrae develop in the nuclear envelope. In the multicentriolar form, however, each interphase nucleus generally is associated with two or three sets of centrioles. Consequently, during mitosis each half-spindle is associated with two or three sets. These centrioles are not necessarily all associated with the fenestrae at the spindle poles, but one or more sets are frequently associated with the nuclear membrane, more or less remote from the nuclear poles. However, the spindle in this multicentriolar form remains essentially bipolar. Cleavage generally results in zoospores with two, four or six flagella. The behaviour of the extra centrioles during the cell cycle and their possible relationship with centrosomes are discussed. 相似文献
6.
This investigation describes the cytology of the ulotrichalean genera Ulothrix and Stigeoclonium. Cellular organization is similar to the degree that interphase cells of the 2 genera cannot be distinguished with certainly. In Stigeoclonium, the nuclear envelope becomes disrupted at the end of prophase, and centrioles enter the nucleoplasm. At metaphase the nuclear envelope is again intact, and some of the spindle tubules appear to be contiguous with the nuclear envelope. The spindle in Ulothrix is essentially open with, no attachment of spindle tubules to the nuclear envelope and with, centrioles on the spindle-cytoplasm interface at the spindle poles. Spindle poles are blunt in Stigeoclonium and pointed in Ulothrix. Cytokinesis is by cell plate formation in both genera, but there is no phragmoplast. 相似文献
7.
Dr. J. D. Pickett-Heaps 《Protoplasma》1970,70(3-4):325-347
Summary Asexual reproduction inKirchneriella lunaris involves autospore formation. After an initial mitosis, the curved cell cleaves to a variable extent, and then the nuclei divide again; finally the cytoplasm is partitioned into four around each nucleus. Rudimentary centrioles appear prior to the first mitosis; centriole complexes then become associated with a developing sheath of extranuclear microtubules at prophase; fenestrae appear at the poles through which both microtubules and centrioles migrate, preceding intranuclear spindle formation. The nucleus meanwhile is enveloped by a perinuclear layer of endoplasmic reticulum which is also interposed between the golgi body and nuclear envelope. Chromosome separation is accompanied by considerable spindle elongation. Finally the reforming nuclear envelope excludes both centriole complex and interzonal spindle apparatus from daughter nuclei. Cleavage is preceded by i) nuclear movement to the cell center, ii) movement of centriole complexes around daughter nuclei until they are opposite one another, and iii) the concurrent formation of a system of transverse microtubules extending across the cell. Other microtubules encircle the cell predicting the cleavage plane. A septum then appears amongst these cytokinetic microtubules, possibly derived from the plasmalemma; it extends across the cell too, through the cleaving peripheral chloroplast. Secondary mitoses follow (as above) during which this septum may be partially resorbed. Finally this septum is reformed, if necessary, and two other septa appear (as above) to quadripartition the cell. Mitotic and cytokinetic structures in this algae are briefly compared with some others. 相似文献
8.
During intranuclear mitosis in plasmodia of Physarum polycephalum the primordium of spindle microtubules which is a somewhat electron opaque, amorphous structure with fibrous or granular elements, occurs in the center of early prophase, 20 to 30 minutes before metaphase. Then, the primordium seems to divide into two parts. Spindle microtubules develop radially from the primordia of spindle microtubules. These spindle microtubules increase in number and length during prophase. Spindle microtubules are completed in about five minutes before metaphase. The nuclear envelope remains intact during prophase, but after metaphase it breaks at the polar regions. The nuclear envelope of the daughter nucleus is re-formed from the original nuclear envelope.The authors wish to express their thanks Professor K. Ueda for valuable advice. They would also like to thank Professor N. Kamiya of Osaka University who kindly supplied the material (Physarum polycephalum) used in the present study. 相似文献
9.
Urs-Peter Roos 《Chromosoma》1973,40(1):43-82
Rat kangaroo (PtK2) cells were fixed and embedded in situ. Cells in mitosis were studied with the light microscope and thin sections examined with the electron microscope. Pericentriolar, osmiophilic material, rather than the centrioles, is probably involved in the formation of astral microtubules during prophase. Centriole migration occurs during prophase and early prometaphase. The nuclear envelope ruptures first in the vicinity of the asters. Nuclear pore complexes disintegrate as envelope fragments are dispersed to the periphery of the mitotic spindle. Microtubules invade the nucleus through gaps of the fragmented envelope. The number of microtubules and the degree of spindle organization increase during prometaphase and are maximal at metaphase. At this stage, chromosomes are aligned on the spindle equator, sister kinetochores facing opposite poles. Cytoplasmic organelles are excluded from the spindle. Prominent bundles of kinetochore microtubules converge towards the poles. Spindles in cold-treated cells consist almost exclusively of kinetochore tubules. Separating daughter chromosomes in early anaphase are connected by chromatin strands, possibly reflecting the rupturing of fibrous connections occasionally observed between sister chromatids in prometaphase. Breakdown of the spindle progresses from late anaphase to telophase, except for the stem bodies. Chromosomes decondense to form two masses. Nuclear envelope reconstruction, probably involving endoplasmic reticulum, begins on the lateral faces. Nuclear pores reappear on membrane segments in contact with chromatin. Microtubules are absent from reconstructed daughter nuclei.This report is to a large part based on a dissertation submitted by the author to the Graduate Council of the University of Florida in partial fulfillment of the requirements for the degree of Doctor of Philosophy. 相似文献
10.
THE FINE STRUCTURE OF MITOSIS IN RAT THYMIC LYMPHOCYTES 总被引:25,自引:22,他引:3
The fine structure of rat thymic lymphocytes from early prophase to late telophase of mitosis is described, using material fixed at pH 7.3 either in 1 per cent OsO4 or in glutaraldehyde followed by 2 per cent OsO4. The structure of the centriolar complex of interphase thymocytes is analyzed and compared with that of centrioles during division. The appearance of daughter centrioles is the earliest clearly recognizable sign of prophase. Daughter centrioles probably retain a secondary relation to the primary centriole, while the latter appears to be related, both genetically and spatially, to the spindle apparatus. The nuclear envelope persists in recognizable form to help reconstitute the envelopes of the daughter nuclei. Ribosome bodies (dense aggregates of ribosomes) accumulate, beginning at late prophase, and are retained by the daughter cells. Cytokinesis proceeds by formation of a ribosome-free plate at the equator with a central plate of vesicles which may coalesce to form the new plasma membrane of the daughter cells. Stages in the formation of the midbody are illustrated. 相似文献
11.
Summary Mitosis in the plasmodium ofEchinostelium minutum is intranuclear (closed spindle) and centrioles are not present at the spindle poles. The nuclear envelope remains essentially intact throughout mitosis with polar fenestrae appearing in anaphase and persisting through telophase. During anaphase there is a shortening in the distance of the chromosomes to the poles followed by a further separation of the poles. Zippering of microtubules may be the basis for these two anaphasic movements. During telophase the polar MTOCs are extruded into the cytoplasm through the polar fenestrae prior to reconstitution of the nuclear envelope. It is proposed that during sporulation such MTOCs are responsible for the differentiation of the centrioles which subsequently persist in the myxamoebal phase of this species.Based on the doctoral dissertation of the first author presented to the Department of Botany, University of Washington, Seattle, WA 98195, U.S.A. 相似文献
12.
Mitosis in vegetative cells of the siphonocladalean algaBoergesenia forbesii (Harvey) Feldmann was investigated mainly by electron microscopy. The mitotic spindle was centric and closed. The interphase
nucleus contained a spherical nucleolus. The nucleolus was slightly dispersed at prophase, but nucleolar materials remained
during nearly all stages of mitosis. Kinetochores were evident on chromosomes. The polar regions of nuclear envelope had no
fenestrae during mitosis. Anaphase separation of the chromosomes was asynchronous. Elongation of interzonal spindle at telophase
separated the two daughter nuclei widely. The ultrastructural features of mitosis inB. forbesii revealed by the present investigation are compared with those of other siphonous and siphonocladous algae in the Ulvophyceae. 相似文献
13.
Ultrastructure of Draparnaldia glomerata (Chaetophorales, Chlorophyceae) II. Mitosis and cytokinesis
The mitosis and cytokinesis of Draparnaldia glomerata as examined here by transmission electron microscopy are in many aspects similar to those described earlier for other chaetophoralean algae. The standard chaetophoralean model of the mechanism of mitosis/cytokinesis is described in detail. Characteristic in this pattern is the movement of sets of centrioles towards the nuclear poles followed by a proliferation of extranuclear microtubules at prophase, the (partial) fusion of centrioles with the spindle poles at metaphase and anaphase, the simultaneous separation of chromosomes apparently caused by both spindle elongation and shortening of the chromosomal microtubules at anaphase, the expulsion of the centrioles by daughter nuclei and finally the non–persistent spindle at telophase. Cytokinesis takes place by formation of a cell plate associated with phycoplast microtubules. The possible function of the phycoplast in cytokinesis in Draparnaldia is discussed. 相似文献
14.
D. B. Gromov 《Protoplasma》1985,126(1-2):130-139
Summary The fine structure ofAmoeba proteus nuclei has been studied during interphase and mitosis. The interphase nucleus is discoidal, the nuclear envelope is provided with a honeycomb layer on the inside. There are numerous nucleoli at the periphery and many chromatin filaments and nuclear helices in the central part of nucleus.In prophase the nucleus becomes spherical, the numerous chromosomes are condensed, and the number of nucleoli decreases. The mitotic apparatus forms inside the nucleus in form of an acentric spindle. In metaphase the nuclear envelope loses its pore complexes and transforms into a system of rough endoplasmic reticulum cisternae (ERC) which separates the mitotic apparatus from the surrounding cytoplasm; the nucleoli and the honeycomb layer disappear completely. In anaphase the half-spindles become conical, and the system of ERC around the mitotic spindle persists. Electron dense material (possibly microtubule organizing centers—MTOCs) appears at the spindle pole regions during this stage. The spindle includes kinetochore microtubules attached to the chromosomes, and non-kinetochore ones which pierce the anaphase plate. In telophase the spindle disappears, the chromosomes decondense, and the nuclear envelope becomes reconstructed from the ERC. At this stage, nucleoli can already be revealed with the light microscope by silver staining; they are visible in ultrathin sections as numerous electron dense bodies at the periphery of the nucleus.The mitotic chromosomes consist of 10 nm fibers and have threelayered kinetochores. Single nuclear helices still occur at early stages of mitosis in the spindle region. 相似文献
15.
Waheeb K. Heneen 《Chromosoma》1970,29(1):88-117
Dividing cells in monolayers of the rat-kangaroo (Potorous tridactylis) cell line Pt-K1 have large spindles and are flat, thus making possible studies of interactions between the achromatic and chromatic parts of the mitotic apparatus during the cell cycle. At prophase, asters and centrioles seem to exert pressure on the nuclear membrane leading to its rupture and penetrance of the centrioles. Apparently, the long axis of the spindle is shorter than the nuclear diameter. What appears as persistent, large portions of the nuclear membrane were observed in some metaphase and anaphase cells. Such a condition might also indicate an arrested mitosis. The midbody, which was often bipartite, was found to be of a ribonucleoprotein nature. — Three-group metaphases were of common occurrence and might represent early stages of chromosome orientation preceding the final alignment of the chromosomes on the equatorial plate. They could also be an expression of an anomalous condition as a result of mitotic arrest during prometaphase owing to spindle inactivation or breakage, errors in centromere-spindle attachments, interference with chromosome movement, or a duplicated centriolar constitution. Most of these aberrations could be attributed to the flatness of dividing cells, which might also bring about the failure of centriole separation and spindle organization in prometaphase stages, as well as multipolar mitosis.De novo organization of half spindles might take place in cells with ruptured spindles. Anaphase cells showing signs of a previous three-group orientation were rare. — Multipolar mitoses were prevalent mainly in cells with high chromosome numbers. They were often star-shaped with the chromosomes oriented between opposite and adjacent poles, and rarely as end-to-end associations of spindles. Apparently, one or more centrioles might share a common polar region. Multipolar configurations have either a mono- or multinuclear origin. Nuclei usually enter division synchronously in binucleate cells and the spindles become organized between centrioles associated with individual or different nuclei. 相似文献
16.
17.
SYNOPSIS. Zoosporulation in Labyrinthula sp. in monoxenic culture was initiated by aggregation of spindle cells into reticulate sori. The spindle cells then changed into rounded or oval cells and formed, de novo, 2 pairs of centrioles at opposite sides of each nucleus. A pair of granular aggregates (protocentrioles) ~ 240 mμ in diameter served as precursor bodies during centriole formation. Spindle microtubules around the prophase nucleus connected the pairs of centrioles but were not found in the nucleoplasm until nuclear envelope fragmentation occurred. Prophase nuclei of uninucleated sporangia contained synaptinemal complexes; therefore, meiosis is presumed to occur. The envelope fragments moved toward the centrioles and regrouped to form the nuclear membranes of the daughter cells. Alternating nuclear and cytoplasmic divisions subdivided the preparation into 8 cells which differentiated into laterally biflagellated zoospores. Flagellar development involved growth of the kinetosome microtubules into a bud which formed over the kinetosome tangential to the cell surface. Kinetosomes were derived directly from centrioles with little differentiation other than addition of an electron-dense core to the lumen of the centriole. Zoospore ultrastructure included a stigma comprised of a row of electron-dense granules located slightly under the plasmalemma and posterior to the pair of kinetosomes. A single row of 17–21 microtubules lay parallel to the stigma granules, one or more being connected to the anterior kinetosome. A striated fiber apparatus similar to that found in some phytoflagellates connected the midregions of the kinetosomes. Fibers 1.0–1.2 μ long were attached to the plasmalemma around the base of the anterior flagellum. Zoospores settled on the substrate and differentiated directly into spindle cells. Since synaptinemal complexes were observed the planonts are probably haploid zoospores and probably not gametes since planogametic copulation was not observed. 相似文献
18.
The role of microtubules and microfilaments in the micronucleus ofParamecium bursaria during mitosis
Summary A unique spindle apparatus develops during mitosis in the micronucleus ofParamecium bursaria. During interphase the micronucleus contains short microtubule profiles and clumps of condensed chromatin. Throughout mitosis the nuclear envelope remains intact. During prophase, cup-shaped structures termed microlamellae develop in close association with regions of condensed chromatin. Each micromella consists of an outer sublamella, an inner sublamellae, and ring-shaped structures termed microsepta that join the two sublamellae. Microtubules elongate parallel to the division axis. During metaphase, the microlamellae appear to act as kinetochorelike structures that aid in the alignment of the chromosomes. The microlamellae appear conical and join to a meshwork of microfilaments at their apices. Further toward the polar regions the microfilaments join with microtubules that converge and terminate near the nuclear envelope. During metaphase-anaphase and anaphase the chromosomes are apparently moved by the microfilaments pulling on the kinetochorelike microlamellae. Also during metaphase-anaphase, extranuclear microtubules join the nuclear envelope of the micronucleus to microtubule elements of the cell cortex. By anaphasetelophase, microlamellae and the microfilament meshwork degenerate and microtubules represent the only spindle elements. The evidence of this report supports the hypothesis that microfilaments can participate with microtubules in the movement of chromosomes.This report is part of a Ph.D. Thesis presented by the senior author at Fordham University. 相似文献
19.
Summary Immunofluorescence studies on microtubule arrangement during the transition from prophase to metaphase in onion root cells are presented. The prophase spindle observed at late preprophase and prophase is composed of microtubules converged at two poles near the nuclear envelope; thin bundles of microtubules are tracable along the nuclear envelope. Prior to nuclear envelope breakdown diffuse tubulin staining occurs within the prophase nuclei. During nuclear envelope breakdown the prophase spindle is no longer identifiable and prominent tubulin staining occurs among the prometaphase chromosomes. Patches of condensed tubulin staining are observed in the vicinity of kinetochores. At advanced prometaphase kinetochore bundles of microtubules are present in some kinetochore regions. At metaphase the mitotic spindle is mainly composed of kinetochore bundles of microtubules; pole-to-pole bundles are scarce. Our observations suggest that the prophase spindle is decomposed at the time of nuclear envelope breakdown and that the metaphase spindle is assembled at prometaphase, with the help of kinetochore nucleating action. 相似文献
20.
ROBERT EDWARD LEE CYNTHIA MILLER-HUGHES PAUL KUGRENS 《The Journal of eukaryotic microbiology》1993,40(3):377-383
ABSTRACT. Mitosis and cytokinesis in Katablepharis ovalis , a colorless flagellate, was investigated. Two new flagella are produced prior to prophase, resulting in a motile quadriflagellate cell during mitosis. the inner array of microtubules of the feeding apparatus disappears before prophase begins. the nuclear envelope disperses during prophase, apparently being converted into rough endoplasmic reticulum. the chromatin condenses and the nucleolus disperses with spindle microtubules appearing oriented perpendicular to the longitudinal axis of the cell. At metaphase, the chromatin is condensed as a single disc-shaped mass and rough endoplasmic reticulum flanks the chromatin mass on each side. Groups of spindle microtubules pass through tunnels in the rough endoplasmic reticulum and through electron-translucent areas of the chromatin. the spindle microtubules end at a number of minipoles in the cytoplasm. Vesicles, ribosomes, mitochondria and endoplasmic reticulum migrate among the spindle microtubules. There is no polar body or any electrondense area associated with the spindle poles. the basal bodies of the flagella remain attached to the axonemes and do not participate in mitosis. In anaphase, the chromatin separates and migrates to the poles. During telophase, the nuclear envelope reforms from the rough endoplasmic reticulum and the nucleoli reappear. the spindle microtubules are persistent during telophase. Cytokinesis occurs by longitudinal fission, starting at the anterior end and progressing posteriorly. Cytokinesis may be driven by elongation of the spindle microtubules since there is no visible structure associated with the furrowing. 相似文献