Photosynthetic and Stomatal Responses of the Grey Mangrove, Avicennia marina, to Transient Salinity Conditions

Measurements of gas exchange characteristics were made on intact, attached leaves of hydroponically grown seedlings of Avicennia marina (Forstk.) Vierh. var australasica (Walp.) Moldenke as the NaCl concentration of the culture solution was varied by step changes of 50 millimolar NaCl every 2nd day from 50 to 500 to 50 millimolar NaCl. The CO₂ assimilation rate, stomatal conductance, intercellular CO₂ concentration, and evaporation rate decreased at salinities above 250 millimolar NaCl and recovered substantially upon return to the original salinity.

The assimilation rate was measured as a function of the intercellular CO₂ concentration [A(c_i) curve]. The lower linear portion of this curve was insensitive to variation in salinity, whereas the upper nonlinear portion declined with increasing salinity, indicating a reduction in the capacity for CO₂ assimilation which recovered upon return to the original salinity. Stomatal conductance changed such that the intercellular CO₂ concentration measured under normal atmospheric conditions occurred in the transition between the lower, linear and upper nonlinear portions of the A(c_i) curve. Thus, stomatal conductance and photosynthetic capacity together co-limited the assimilation rate. The changes in gas exchange characteristics were such that water loss was minimal relative to carbon gain.

     

Silicon Alleviation of Cadmium Toxicity in Mangrove (Avicennia marina) in Relation to Cadmium Compartmentation

Mangrove plants seem to be highly tolerant of high levels of heavy-metal pollution. Recently, some researchers have focused on the mechanisms involved in their metal uptake and tolerance. However, the important mechanisms involved are still only partly understood. This investigation studied whether silicon (Si) affected cadmium (Cd) subcellular distribution in the leaves and root tips of Avicennia marina (Forsk.) Vierh seedlings, resulting in the amelioration of the toxicity of Cd. The results showed that Si partly overcame the reduction in growth due to Cd. This amelioration was correlated with a reduction in Cd uptake and alteration of Cd subcellular distribution. The mechanisms of Si amelioration of Cd stress were tissue dependent. In the leaves and root tips, Si reduced Cd concentration in subcellular fractions, Cd mobility, and the concentration of biologically active Cd in the cell wall active space. Si did not change the distribution of Cd between compartments in the leaves, but it increased the proportion of Cd in the cell walls and reduced the proportion of Cd in the symplast of the root tips.     

Photosynthetic and Stomatal Responses of Two Mangrove Species, Aegiceras corniculatum and Avicennia marina, to Long Term Salinity and Humidity Conditions

Gas exchange characteristics were studied in two mangrove species, Aegiceras corniculatum (L.) Blanco and Avicennia marina (Forstk.) Vierh. var australasica (Walp.) Moldenke, grown under a variety of salinity and humidity conditions. The assimilation rate was measured as a function of the intercellular CO₂ concentration [A(c_i) curve]. The photosynthetic capacity decreased with increase in salinity from 50 to 500 millimolar NaCl, as shown by decline in both the initial linear slope and the upper plateau of the A(c_i) curve, with A. corniculatum being the more sensitive species. The decline in photosynthetic capacity was enhanced by increase in the leaf to air vapor pressure difference from 6 to 24 millibars, but this treatment caused a decrease in only the upper plateau of the A(c_i) curve. Stomatal conductance was such that the intercellular CO₂ concentration obtaining under normal atmospheric conditions occurred near the transition between the lower linear and upper plateau portions of the A(c_i) curves. Thus, stomatal conductance and photosynthetic capacity together co-limited the assimilation rate, which declined with increasing salinity and decreasing humidity. The marginal water cost of carbon assimilation was similar in most treatments, despite variation in the water loss/carbon gain ratio.     

Identification of miRNAs and Their Target Genes in Peach (Prunus persica L.) Using High-Throughput Sequencing and Degradome Analysis

MicroRNAs play critical roles in various biological and metabolic processes. The function of miRNAs has been widely studied in model plants such as Arabidopsis and rice. However, the number of identified miRNAs and related miRNA targets in peach (Prunus persica) is limited. To understand further the relationship between miRNAs and their target genes during tissue development in peach, a small RNA library and three degradome libraries were constructed from three tissues for deep sequencing. We identified 117 conserved miRNAs and 186 novel miRNA candidates in peach by deep sequencing and 19 conserved miRNAs and 13 novel miRNAs were further evaluated for their expression by RT-qPCR. The number of gene targets that were identified for 26 conserved miRNA families and 38 novel miRNA candidates, were 172 and 87, respectively. Some of the identified miRNA targets were abundantly represented as conserved miRNA targets in plant. However, some of them were first identified and showed important roles in peach development. Our study provides information concerning the regulatory network of miRNAs in peach and advances our understanding of miRNA functions during tissue development.     

Soil and Rhizosphere Associated Fungi in Gray Mangroves(Avicennia marina) from the Red Sea—A Metagenomic Approach

Covering a quarter of the world's tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea gray mangroves(Avicennia marina) remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from gray mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum(76%–85%), while Basidiomycota was less abundant(14%–24%), yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the gray mangroves of the Red Sea, and show that they are significantly richer than previously reported.     

Effects of shoot position on shoot and leaf morphology of Avicennia marina in the hyperarid Red Sea coastal region of Egypt

We examined the effects of shoot position on shoot growth and morphology of Avicennia marina (Forssk.) Vierh. in the Red Sea coastal region of Egypt. To determine differences in morphological characteristics, we collected shoots from the upper and lower canopies of A. marina individuals in the wild and compared the morphological characteristics of these shoots. The study plot was established in an A. marina mangrove forest. Heights and diameters of individual trunks (n = 14) in the plot were measured at ground level. Then, five shoots with young but fully expanded leaves were collected from the upper and lower canopies of the individuals. We measured shoot length, and dry weight and also area, dry weight, thickness, and Soil Plant Analysis Development (SPAD) value of collected leaves. Our measurements showed that leaf area, dry weight, specific leaf area, and SPAD value of leaves from the upper canopy were smaller than those of lower-canopy leaves in most individuals. From the differences in traits between upper and lower leaves, we concluded that leaves in the upper canopy are typically adapted to high light levels, whereas leaves in the lower canopy exhibit adaptations to low light conditions. In addition, soil-water salinity at the study site was far higher than the optimum salinity for A. marina. Hence, it is also suggested the salinity level at this site may have influenced the reduced leaf size in the upper canopy.     

Identification of the Conserved and Novel miRNAs in Mulberry by High-Throughput Sequencing

miRNAs are a class of non-coding endogenous small RNAs. They play vital roles in plant growth, development, and response to biotic and abiotic stress by negatively regulating genes. Mulberry trees are economically important species with multiple uses. However, to date, little is known about mulberry miRNAs and their target genes. In the present study, three small mulberry RNA libraries were constructed and sequenced using high-throughput sequencing technology. Results showed 85 conserved miRNAs belonging to 31 miRNA families and 262 novel miRNAs at 371 loci. Quantitative real-time PCR (qRT-PCR) analysis confirmed the expression pattern of 9 conserved and 5 novel miRNAs in leaves, bark, and male flowers. A total of 332 potential target genes were predicted to be associated with these 113 novel miRNAs. These results provide a basis for further understanding of mulberry miRNAs and the biological processes in which they are involved.     

Growth and establishment of mangrove (Avicennia marina) on the coastlines of Kuwait

Mangrove (Avicennia marina) communities constitute an importantcomponent of the renewable natural vegetation resources of the coastalzones of the Arabian Gulf south of Kuwait. Attempts to introduce themangrove into the intertidal zones of the coastline of Kuwait are underwaybecause of the expected favorable environmental impact of this plant, andbecause of its moderating influence on the local climate and enhancementof the aesthetic and recreational value of the coastlines and fisheryresources. Propagules were collected from natural stands of mangrove inBahrain and the United Arab Emirates. Silvicultural practices were followedto produce healthy plants after growing of propagules in the greenhouse.Observations indicated that there is a positive correlation betweenpropagule weight and the length of the seedling stem. Outplanting wasdone in two sites in the intertidal zone around the Sulabikhat Bay, Kuwait.Data on plant survival showed that the most suitable level for successfulestablishment of mangrove was around or below the 3-meter contour tidalline. After 7 years of growth, plants reached a mean height of 250 cm,which is close to the height of their parent trees in Bahrain. The U.A.E.stock plants reached 288 cm after 5 years of growth in Kuwait. Plantsflowered and produced viable seeds. The biological feasibility ofestablishing mangrove plantations in the intertidal flats of Kuwait's coastlineswas documented from the field observation of the experimental plantations.Studies to document the environmental impact of the introduction areunderway.     

Discovery and Analysis of MicroRNAs in Leymus chinensis under Saline-Alkali and Drought Stress Using High-Throughput Sequencing

Leymus chinensis (Trin.) Tzvel. is a perennial rhizome grass of the Poaceae (also called Gramineae) family, which adapts well to drought, saline and alkaline conditions. However, little is known about the stress tolerance of L. chinensis at the molecular level. microRNAs (miRNAs) are known to play critical roles in nutrient homeostasis, developmental processes, pathogen responses, and abiotic stress in plants. In this study, we used Solexa sequencing technology to generate high-quality small RNA data from three L. chinensis groups: a control group, a saline-alkaline stress group (100 mM NaCl and 200 mM NaHCO₃), and a drought stress group (20% polyethylene glycol 2000). From these data we identified 132 known miRNAs and 16 novel miRNAs candidates. For these miRNAs we also identified target genes that encode a broad range of proteins that may be correlated with abiotic stress regulation. This is the first study to demonstrate differentially expressed miRNAs in L. chinensis under saline-alkali and drought stress. These findings may help explain the saline-alkaline and drought stress responses in L. chinensis.