共查询到20条相似文献,搜索用时 15 毫秒
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Rita J. S. Phillips 《Genetics》1966,54(2):485-495
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Mengqian Ye Mengru Wang Yijia Feng Huiping Shang Yuwen Yang Lanxin Hu Muran Wang Serhii Vakal Xiangxiang Lin Jiangfan Chen Wu Zheng 《Purinergic signalling》2023,19(1):135
The choroid plexus (CP) is one of the key gateways regulating the entry of peripheral immune cells into the CNS. However, the neuromodulatory mechanisms of maintaining its gateway activity are not fully understood. Here, we identified adenosine A2A receptor (A2AR) activity as a regulatory signal for the activity of CP gateway under physiological conditions. In association with a tightly closed CP gateway, we found that A2AR was present at low density in the CP. The RNA-seq analysis revealed that the A2AR antagonist KW6002 affected the expression of the cell adhesion molecules’ (CAMs) pathway and cell response to IFN-γ in the CP. Furthermore, blocking or activating A2AR signaling in the CP resulted in a decreased and an increased, respectively, expression of lymphocyte trafficking determinants and disruption of the tight junctions (TJs). Furthermore, A2AR signaling regulates the CP permeability. Thus, A2AR activity in the CP may serve as a therapeutic target for remodeling the immune homeostasis in the CNS with implications for the treatment of neuroimmunological disorders.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11302-022-09847-5. 相似文献
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G B Forbes 《BMJ (Clinical research ed.)》1998,317(7153):262
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The antifungal antibiotic ramihyphin A, isolated fromFusarium solani strain S-435 in 1974, was shown to be identical with cyclosporin A. 相似文献
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Demonstration of the hybrid hemoglobin 2 A A S 总被引:2,自引:0,他引:2
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Sakayu Shimizu Keijiro Miyata Yoshiki Tani Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(3):615-619
A new process has been described for the preparation of coenzyme A of high purity from the cultured broth of Brevibacterium ammoniagenes IFO 12071. The product was obtained in a high yield by the use of Duolite S–30, charcoal, and Dowex 1×2, and identified chemically and enzymatically. This method is simple, rapid, and compact, requires no special equipment, and has been shown to be adaptable for preparing large amounts of highly pure coenzyme A. 相似文献
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Rami Almog 《Biophysical chemistry》1983,18(4):391-395
The small positive elliplicity near 239 nm in the CD spectrum of RNase has been investigated as a function of pH. Theoretical calculations using CD parameters representing buried or exposed tyrosine residues have been carried out. A comparison of the theoretical calculations with experimental data suggests that the changes in the band's intensity, as a function of pH, arise mainly from electronic transitions associated with the tyrosine residues. The buried tyrosine residues are the major contributors to the ellipticity in this region at neutral pH. At higher pH contributions from exposed residurs are also observed. 相似文献
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Phagolessin A58, an antibiotic substance active against a number of bacterial viruses, was studied for activity against the seven T phages. Only three of the seven phages—T1, T3, and T7—proved to be sensitive to the antibiotic. The antibiotic caused a direct, apparently irreversible inactivation of free phage particles. A study of the properties of the inactivated phage particles showed that the particles retained the ability to kill host cells and to exert mutual exclusion against an unrelated phage after infectivity was lost. There was a progressive loss in these two properties when higher concentrations of antibiotic were used to inactivate the phage. Results with inactivated T3 and T7 revealed that these two properties—the ability to kill host cells and to exclude an unrelated phage—were lost at a different rate. They were, therefore, presumed to be different properties of these particular phage particles. The inactivation of phage by phagolessin A58 was inhibited by desoxyribose nucleic acid and to a lesser extent by ribose nucleic add. Cytosine, thymine, adenine, guanine, and cysteine failed to inhibit the reaction. 相似文献
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Alisha D Caliman Sara E Swift Yi Wang Yinglong Miao J Andrew McCammon 《Protein science : a publication of the Protein Society》2015,24(6):1004-1012
The activation/deactivation processes for G-protein coupled receptors (GPCRs) have been computationally studied for several different classes, including rhodopsin, the β2 adrenergic receptor, and the M2 muscarinic receptor. Despite determined cocrystal structures of the adenosine A2A receptor (A2AAR) in complex with antagonists, agonists and an antibody, the deactivation process of this GPCR is not completely understood. In this study, we investigate the convergence of two apo simulations, one starting with an agonist-bound conformation (PDB: 3QAK)14 and the other starting with an antagonist-bound conformation (PDB: 3EML)11. Despite the two simulations not completely converging, we were able to identify distinct intermediate steps of the deactivation process characterized by the movement of Y2887.53 in the NPxxY motif. We find that Y2887.53 contributes to the process by forming hydrogen bonds to residues in transmembrane helices 2 and 7 and losing these interactions upon full deactivation. Y1975.58 also plays a role in the process by forming a hydrogen bond only once the side chain moves from the lipid interface to the middle of the helical bundle. 相似文献
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Thévenin D Lazarova T Roberts MF Robinson CR 《Protein science : a publication of the Protein Society》2005,14(8):2177-2186
The human adenosine A2A receptor (A2AR) belongs to one of the largest family of membrane proteins, the G-protein coupled receptors (GPCRs), characterized by seven transmembrane (TM) helices. Little is known about the determinants of their structures, folding, assembly, activation mechanisms, and oligomeric states. Previous studies in our group showed that peptides corresponding to all seven TM domains form stable helical structures in detergent micelles and lipid vesicles. However, the peptides behave differently; TM5 is the only peptide to have a ratio [θ]222/[θ]208 obtained by circular dichroism (CD) spectroscopy>1. This finding suggested to us that TM5 might self-associate. In the present study, we investigate the unique properties of the TM5 domain. We performed detailed analyses of TM5 peptide behavior in membrane-mimetic environments using CD spectroscopy, fluorescence spectroscopy and Förster resonance energy transfer, and gel electrophoresis. We find that TM5 peptide has the ability to self-associate to form oligomeric structures in various hydrophobic milieus and that these oligomers are highly resistant to temperature and chemical denaturation. We also find that mutation of the full-length A2AR at position M193, which is located in the fifth TM domain, noticeably alters A2AR monomer: dimer ratio as observed on SDS-PAGE. Our results suggest that parallel association of TM5 dimers may play a role in the known adenosine A2A receptor dimerization. This study represents the first evidence of an individual GPCR transmembrane domain self-association. 相似文献
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G-protein-coupled receptors (GPCRs) must properly insert and fold in the membrane to adopt a stable native structure and become biologically active. The interactions between transmembrane (TM) helices are believed to play a major role in these processes. Previous studies in our group showed that specific interactions between TM helices occur, leading to an increase in helical content, especially in weakly helical TM domains, suggesting that helix–helix interactions in addition to helix–lipid interactions facilitate helix formation. They also demonstrated that TM peptides interact in a similar fashion in micelles and lipid vesicles, as they exhibit relatively similar thermal stability and α-helicity inserted in SDS micelles to that observed in liposomes. In this study, we perform an analysis of pairwise interactions between peptides corresponding to the seven TM domains of the human A2A receptor (A2AR). We used a combination of Förster resonance energy transfer (FRET) measurement and circular dichroism (CD) spectroscopy to detect and analyze these interactions in detergent micelles. We found that strong and specific interactions occur in only seven of the 28 possible peptide pairs. Furthermore, not all interactions, identified by FRET, lead to a change in helicity. Our results identify stabilizing contacts that are likely related to the stability of the receptor and that are consistent with what is known about the three-dimensional structure and stability of rhodopsin and the β2 adrenergic receptor. 相似文献
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对"功能反应函数为x的食饵——捕食系统的定性分析"一文的注记 总被引:1,自引:0,他引:1
重新分析了文[1]所讨论过的功能反应函数为x的捕食系统(1),分析了此系统在第一象限内轨线的拓朴结构,证明了系统(1)的唯一正平衡点如果不稳定,则存在唯一(稳定)极限环;如果稳定,则全局稳定于此正平衡点,纠正了文[1]中关于系统(1)极限环的存在性、稳定性等方面的一些结论. 相似文献