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1.
Four yellow pigmented strains (91A-561T, 91A-576, 91A-593T, and JM-1085T) isolated from plant materials, showed 97.2–98.7 % 16S rRNA gene sequence similarities among each other and were studied in a polyphasic approach for their taxonomic allocation. Cells of all four isolates were rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the four bacteria had highest sequence similarities to Chryseobacterium formosense (97.2–98.7 %), Chryseobacterium gwangjuense (97.1–97.8 %), and Chryseobacterium defluvii (94.6–98.0 %). Sequence similarities to all other Chryseobacterium species were below 97.5 %. Fatty acid analysis of the four strains showed Chryseobacterium typical profiles consisting of major fatty acids C15:0 iso, C15:0 iso 2-OH/C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences. DNA–DNA hybridizations with type strains of C. gwangjuense, C. formosense, and C. defluvii resulted in values below 70 %. Isolates 91A-561T and 91A-576 showed DNA–DNA hybridization values >80 % indicating that they belonged to the same species; but nucleic acid fingerprinting showed that the two isolates represent two different strains. DNA–DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 91A-561T and 91A-576 represent a novel species, for which the name Chryseobacterium geocarposphaerae sp. nov. (type strain 91A-561T=LMG 27811T=CCM 8488T) is proposed. Strains 91A-593T and JM-1085T represent two additional new species for which we propose the names Chyrseobacterium zeae sp. nov. (type strain JM-1085T=LMG 27809T, =CCM 8491T) and Chryseobacterium arachidis sp. nov. (type strain 91A-593T=LMG 27813T, =CCM 8489T), respectively. 相似文献
2.
Mariane Schmidt Anders Priemé Anders Johansen Peter Stougaard 《Extremophiles : life under extreme conditions》2012,16(2):297-305
Three novel Gram-positive, endospore-forming bacteria were isolated from a cold and alkaline environment. Phylogenetic analysis
showed that the strains were almost identical, and that they were related to Natronobacillus azotifigens 24KS-1T (95.8% identity), Paraliobacillus quinghaiensis YIM-C158T (95.1%), Paraliobacillus ryukyuensis O15-7T (94.5%), and Halolactibacillus miurensis M23-1T (93.9%). The isolates produced amylase, α-galactosidase, β-galactosidase, and β-glucuronidase, and showed optimal growth
at pH 10, at 20°C, and at 2–8% (w/v) NaCl. Major fatty acids were C14:0 (10.6–11.6%), anteiso-C15:0 (25.7–32.7%), C16:1
ω11c (12.2–16.0%), and C16:0 (14.0–20.4%). The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and meso-diaminopimelic acid was found in the cell-wall peptidoglycan. The G+C content was 38.4%. DNA–DNA hybridization between strain
GCM68T and H. miurensis M23-1T was 32.4%, while hybridization to N. azotifigens 24KS-1T, Amphibacillus tropicus Z-7792T, and Paraliobacillus ryukyuensis O15-7T was below 30%. The phylogenetic analysis and G+C content place strain GCM68T in relation to species belonging to Bacillus rRNA group 1, but phylogenetic and physiologic data combined with chemotaxonomic analyses support our proposal for a new
genus, Alkalilactibacillus, gen. nov., with the novel species Alkalilactibacillus ikkensis, sp. nov. (type strain is GCM68T = DSM 19937 = LMG 24405). 相似文献
3.
Sung-Heun Cho Kang Seon Lee Dong-Sung Shin Ji-Hye Han Ki Seok Park Cheon Hee Lee Kwan Ha Park Seung Bum Kim 《Systematic and applied microbiology》2010
The taxonomic positions of five Gram-negative, non-spore-forming and non-motile bacterial strains isolated from the rhizosphere of sand dune plants were examined using a polyphasic approach. The analysis of the 16S rRNA gene sequence indicated that all of the isolates fell into four distinct phylogenetic clusters belonging to the genus Chryseobacterium of the family Flavobacteriaceae. The 16S rRNA gene sequence similarities of isolates to mostly related type strains of Chryseobacterium ranged from 97.5% to 98.5%. All strains contained MK-6 as the predominant menaquinone, and iso-C15:0, iso-C17:0 3-OH and a summed feature of iso-C15:0 2-OH and/or C16:1 ω7c as the dominant fatty acids. Combined phenotypic, genotypic and chemotaxonomic data supported that they represented four novel species in the genus Chryseobacterium, for which the names Chryseobacterium hagamense sp. nov. (type strain RHA2-9T=KCTC 22545T=NBRC 105253T), Chryseobacterium elymi sp. nov. (type strain RHA3-1T=KCTC 22547T=NBRC 105251T), Chryseobacterium lathyri sp. nov. (type strain RBA2-6T=KCTC 22544T=NBRC 105250T), and Chryseobacterium rhizosphaerae sp. nov. (type strain RSB3-1T=KCTC 22548T=NBRC 105248T) are proposed. 相似文献
4.
《Systematic and applied microbiology》2023,46(1):126385
Four strains, designated as C-2, C-17T, C-39T and Ch-15, were isolated from farmed rainbow trout samples showing clinical signs during an investigation for a fish-health screening study. The pairwise 16S rRNA gene sequence analysis showed that strain C-17T shared the highest identity level of 98.1 % with the type strain of Chryseobacterium piscium LMG 23089T while strains C-2, C-39T and Ch-15 were closely related to Chryseobacterium balustinum DSM 16775T with an identity level of 99.3 %. A polyphasic approach involving phenotypic, chemotaxonomic and genome-based analyses was employed to determine the taxonomic provenance of the strains. The overall genome relatedness indices including dDDH and ANI analyses confirmed that strains C-2, C-17T, C-39T and Ch-15 formed two novel species within the genus Chryseobacterium. Chemotaxonomic analyses showed that strains C-17T and C-39T have typical characteristics of the genus Chryseobacterium by having phosphatidylethanolamine in their polar lipid profile, MK-6 as only isoprenoid quinone and the presence of iso-C15:0 as major fatty acid. The genome size and G + C content of the strains ranged between 4.4 and 5.0 Mb and 33.5 – 33.6 %, respectively. Comprehensive genome analyses revealed that the strains have antimicrobial resistance genes, prophages and horizontally acquired genes in addition to secondary metabolite-coding gene clusters. In conclusion, based on the polyphasic analyses conducted on the present study, strains C-17T and C-39T are representatives of two novel species within the genus Chryseobacterium, for which the names Chryseobacterium turcicum sp. nov. and Chryseobacterium muglaense sp. nov. with the type strains C-17T (=JCM 34190T = KCTC 82250T) and C-39T (=JCM 34191T = KCTC 822251T), respectively, are proposed. 相似文献
5.
Huang HY Chen YG Wang YX Liu JH Tang SK Peng Q Wen ML Yu H Cui XL 《Extremophiles : life under extreme conditions》2008,12(6):829-835
A novel Gram-negative, slightly halophilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-C248T, was isolated from a sediment sample collected from a salt-lake in the Qaidam Basin in Qinghai, north-west China. Cells were
non-sporulating short rods, occurring singly or as doublets, motile with peritrichous flagella. Growth occurred with 1–15%
(w/v) NaCl [optimum 2–4% (w/v) NaCl], at pH 6.0–10.0 (optimum pH 7.5) and at 4–35°C (optimum 25–30°C). The major cellular
fatty acids were C18:1
ω7c, C12:0 3-OH, cyclo C19:0
ω8c, C16:0 and C16:1. The predominant respiratory quinone was Q-9 and the genomic DNA G + C content was 58.6 mol%. Phylogenetic analysis based
on 16S rRNA gene sequences indicated that strain YIM-C248T should be assigned to the genus Halomonas. The sequence similarities between the isolate and the type strains of members of the genus Halomonas were in the range of 92.5–97.5%. The combination of phylogenetic analysis, DNA–DNA hybridization data, phenotypic characteristics
and chemotaxonomic differences supported the view that strain YIM-C248T represents a new species of the genus Halomonas, for which the name Halomonas sediminis sp. nov. is proposed, with YIM-C248T (=CCTCC AA 207031 = KCTC 22167) as the type strain.
The GenBank/EMBL/DBBJ accession number for the 16S rRNA gene sequence of strain YIM-C248T is EU135707. 相似文献
6.
The taxonomic position of strain DFH11T, which was isolated from coastal seawater off Qingdao, People’s Republic of China in 2007, was determined. Strain DFH11T comprised Gram-negative, motile, strictly aerobic spirilli that did not produce catalase. Comparative 16S rRNA gene sequence
analysis revealed that strain DFH11T shared ~97.2, 93.3, 91.8, 91.7 and 91.5% sequence similarities with Oleispira antarctica, Spongiispira norvegica, Bermanella marisrubri, Oceaniserpentilla haliotis and Reinekea aestuarii, respectively. DNA–DNA hybridization experiments indicated that the strain was distinct from its closest phylogenetic neighbour,
O. antarctica. The strain grew optimally in 2–3% (w/v) NaCl, at pH 5.0–10.0 (optimally at pH 7.0) and between 0 and 30°C (optimum growth
temperature 28°C). The strain exhibited a restricted substrate profile, with a preference for aliphatic hydrocarbons, that
is consistent with its closest phylogenetic neighbour O. antarctica. Growth of the isolate at different temperatures affected the cellular fatty acid profile. 28°C cultured cells contained
C16:1ω7c and/or iso-C15:0 2-OH (50.4%) and C16:0 (19.2%) as the major fatty acids. However, the major fatty acids of the cells cultured at 4°C were C16:1ω7c and/or C16:1ω6c (40.2%), C16:0 (17.2%) and C17:1ω8c (10.1%). The G+C content of the genomic DNA was 42.7 mol%. Phylogeny based on 16S rRNA gene sequences together with data
from DNA–DNA hybridization, phenotypic and chemotaxonomic characterization revealed that DFH11T should be classified as a novel species of the genus Oleispira, for which the name Oleispira
lenta sp. nov. is proposed, with the type strain DFH11T (=NCIMB 14529T = LMG 24829T). 相似文献
7.
Da-Chun Zhuang Yi-Guang Chen Yu-Qin Zhang Shu-Kun Tang Xiao-Lei Wu Zhou-Cai Tan Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(3):295-301
A novel Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, aerobic bacterium, designated strain JSM
078120T, was isolated from sea water collected from a tidal flat of Naozhou Island, South China Sea. Growth occurred with 1–15% (w/v)
total salts (optimum, 2–4%), at pH 6.0–10.0 (optimum, pH 7.5) and at 4–35°C (optimum, 25–30°C). The major cellular fatty acids
were C18:1
ω9c, C16:0, C12:0 3-OH and C16:1
ω7c. The predominant respiratory quinone was ubiquinone Q-9, and the genomic DNA G + C content was 60.6 mol%. Phylogenetic analysis
based on 16S rRNA gene sequences indicated that strain JSM 078120T should be assigned to the genus Marinobacter, being related most closely to the type strains of Marinobacter segnicrescens (sequence similarity 98.2%), Marinobacter bryozoorum (97.9%) and Marinobacter gudaonensis (97.6%). The sequence similarities between the novel isolate and the type strains of other recognized Marinobacter species ranged from 96.7 (with Marinobacter salsuginis) to 93.3% (with Marinobacter litoralis). The levels of DNA–DNA relatedness between strain JSM 078120T and the type strains of M. segnicrescens, M. bryozoorum and M. gudaonensis were 25.3, 20.6 and 18.8%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics
and chemotaxonomic data supported the view that strain JSM 078120T represents a novel species of the genus Marinobacter, for which the name Marinobacter zhanjiangensis sp. nov. is proposed. The type strain is JSM 078120T (= CCTCC AB 208029T = DSM 21077T = KCTC 22280T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078120T is FJ425903. 相似文献
8.
Yi-Guang Chen Jun Chen Qi-Hui Chen Shu-Kun Tang Yu-Qin Zhang Jian-Wu He Wen-Jun Li Yan-Qi Liu 《Antonie van Leeuwenhoek》2010,98(3):395-401
A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, non-motile, non-sporulating, catalase-positive,
oxidase-negative, aerobic bacterium, designated strain JSM 070026T, was isolated from non-saline forest soil in China. Growth occurred with 0–20% (w/v) NaCl (optimum, 2–4%) and at pH 6.0–10.5
(optimum, pH 8.0) and 5–40°C (optimum, 30°C). Good growth also occurred in the presence of 0–28% (w/v) KCl (optimum, 2–5%)
or 0–25% (w/v) MgCl2·6H2O (optimum, 1–4%). The peptidoglycan type was A4α (l-Lys–Gly–l-Glu). Cell-wall sugars contained mannose and xylose. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Strain
JSM 070026T contained menaquinone 8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol
as the major polar lipids. The DNA G + C content of strain JSM 070026T was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 070026T was a member of the suborder Micrococcineae and most closely related to Yaniella flava YIM 70178T (sequence similarity 99.4%) and Yaniella halotolerans YIM 70085T (97.9%). The three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA–DNA
relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 070026T represents a novel species of the genus Yaniella, for which the name Yaniella soli sp. nov. is proposed. The type strain is JSM 070026T (=DSM 22211T = KCTC 13527T). 相似文献
9.
Yi-Guang Chen Huai-Dong Xiao Shu-Kun Tang Yu-Qin Zhang Entomack Borrathybay Xiao-Long Cui Wen-Jun Li Yan-Qi Liu 《Antonie van Leeuwenhoek》2009,96(3):259-266
A pale yellow-colored, moderately halophilic, Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped,
motile, aerobic bacterium, designated strain JSM 073008T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 1–20% (w/v) total salts
(optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 7.5) and 10–40°C (optimum, 25–30°C). The major cellular fatty acids were C16:0, C16:1
ω7c/iso-C15:0 2-OH and C18:1
ω7c. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The predominant
respiratory quinone was Q-8 and the genomic DNA G + C content was 47.5 mol%. Phylogenetic analysis based on 16S rRNA gene
sequences indicated that strain JSM 073008T should be assigned to the genus Alteromonas, being most closely related to Alteromonas hispanica F-32T (sequence similarity 96.9%), followed by Alteromonas genovensis LMG 24078T (96.6%) and Alteromonas litorea TF-22T (96.4%). The sequence similarities between the novel isolate and the type strains of other recognized Alteromonas species ranged from 95.9% (with Alteromonas stellipolaris ANT 69aT) to 94.5% (with Alteromonas simiduii BCRC 17572T). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the view that strain
JSM 073008T represents a new species of the genus Alteromonas, for which the name Alteromonas halophila sp. nov. is proposed. The type strain is JSM 073008T (=CCTCC AA 207035T = KCTC 22164T).
The authors Yi-Guang Chen and Huai-Dong Xiao have contributed equally to this work. 相似文献
10.
Annukka E. Mäkinen Anna H. Kaksonen Jaakko A. Puhakka 《Extremophiles : life under extreme conditions》2009,13(3):505-510
A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70BT was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50–81°C (optimum
71°C) and 6.2–9.8 (optimum pH 7–7.5), respectively. Growth occurred in the presence 0–2% NaCl (optimum 1% NaCl). Strain 70BT could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used
as electron acceptor. Major whole-cell fatty acids were iso-C15:0, C16:0 DMA (dimethyl acetal), C16:0 and anteiso-C15:0. The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives
of strain 70BT were Thermosediminibacter oceani DSM 16646T (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70BT represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70BT (=DSM 21563 = JCM 15541). 相似文献
11.
12.
Olfa Ben Dhia Thabet Terres Wafa Khelifi Eltaief Jean-Luc Cayol Moktar Hamdi Guy Fauque Marie-Laure Fardeau 《Current microbiology》2011,62(2):486-491
A new moderately halophilic sulfate-reducing bacterium (strain H1T) was enriched and isolated from a wastewater digestor in Tunisia. Cells were curved, motile rods (2–3 x 0.5 μm). Strain H1T grew at temperatures between 22 and 43°C (optimum 35°C), and at pH between 5.0 and 9.2 (optimum 7.3–7.5). Strain H1T required salt for growth (1–45 g of NaCl/l), with an optimum at 20–30 g/l. Sulfate, sulfite, thiosulfate, and elemental sulfur
were used as terminal electron acceptors but not nitrate and nitrite. Strain H1T utilized lactate, pyruvate, succinate, fumarate, ethanol, and hydrogen (in the presence of acetate and CO2) as electron donors in the presence of sulfate as electron acceptor. The main end-products from lactate oxidation were acetate
with H2 and CO2. The G + C content of the genomic DNA was 55%. The predominant fatty acids of strain H1T were C15:0 iso (38.8%), C16:0 (19%), and C14:0 iso 3OH (12.2%), and menaquinone MK-6 was the major respiratory quinone. Phylogenetic analysis of the small-subunit (SSU)
ribosomal RNA (rRNA) gene sequence indicated that strain H1T was affiliated to the genus Desulfovibrio. On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain H1T is proposed to be assigned to a novel species of sulfate reducers of the genus Desulfovibrio, Desulfovibrio legallis sp. nov. (= DSM 19129T = CCUG 54389T). 相似文献
13.
A Gram-negative, aerobic, motile and slightly curved rod-shaped bacterium (BFLP-8T) was isolated from cutaneous mucus of wild long-snouted seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-8T grew at 10–35 °C and pH 5–9 (optimally at 25 °C and pH 7.0) and with 1–6 % (w/v) NaCl (optimally with 2 % NaCl). The predominant respiratory quinone (90 %) was ubiquinone with ten isoprene units (Q-10) and the major fatty acids identified were C18:1 ω7c (54.8 % of the total), C19:0 cyclo ω8c (11.6 %), C16:0 (9.5 %), C18:1 2-OH (7.1 %) and C16:1 ω11c (6.7 %). The G+C content of the DNA was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-8T formed a distinct clade within the family Sneathiellaceae but is not specifically associated with any species in the family. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-8T represents a novel species within a new genus, for which the name Oceanibacterium hippocampi gen. nov., sp. nov. is proposed. The type strain is BFLP-8T (=CECT 7691T = DSM 23444T). 相似文献
14.
Yi-Guang Chen Shu-Kun Tang Yu-Qin Zhang Zhu-Xiang Liu Qi-Hui Chen Jian-Wu He Xiao-Long Cui Wen-Jun Li 《Extremophiles : life under extreme conditions》2010,14(4):397-402
A novel moderately halophilic, alkaliphilic, non-motile, non-sporulating, catalase-positive, oxidase-negative, aerobic, coccus-shaped,
Gram-positive bacterium, designated strain JSM 071043T, was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Growth occurred with
0.5–20% (w/v) NaCl (optimum 5–10%) at pH 6.5–10.5 (optimum pH 8.5) and at 10–40°C (optimum 25–30°C). Good growth also occurred
in the presence of 0.5–20% (w/v) KCl (optimum 5–8%) or 0.5–25% (w/v) MgCl2·6H2O (optimum 5–10%). The peptidoglycan type was A4α (l-Lys–l-Ala–l-Glu) and major cell-wall sugars were tyvelose and mannose. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Strain JSM 071043T contained MK-9 and MK-8 as the predominant menaquinones and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol
as the major polar lipids. The DNA G + C content was 67.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed
that strain JSM 071043T was a member of the suborder Micrococcineae, and was most closely related to Zhihengliuella halotolerans YIM 70185T (sequence similarity 98.9%) and Zhihengliuella alba YIM 90734T (98.2%), and the three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis,
DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 071043T represents a novel species of the genus Zhihengliuella, for which the name Z. salsuginis sp. nov. is proposed. The type strain is JSM 071043T (= DSM 21149T = KCTC 19466T). 相似文献
15.
Three pigmented strains of halophilic archaea, RS94-RS96, were isolated from acidic foamy products of flotation enrichment of potassium minerals (Silvinit Co., Solikamsk, Russia). The cells were gram-negative, nonmotile, pleomorphic ovoids, 1.0−1.5 × 1.5−2.5 μm. The isolates were chemoorganotrophic, obligately aerobic, and catalase-positive. A range of carbohydrates and organic acids was used, as well as amino acids and peptides. The strains were halophiles and thermotolerant neutrophiles. They grew in the media with 15 to 30% NaCl (optimum at 20–22%) and 0.005–0.7 M Mg2+ (0.1–0.2 M), at pH 5.0–8.2 (optimum 7.0–7.2) and 25–55°C (optimum at 35–50°C). The major fatty acids were C16:0, C18:1, C18:0, and C16:1. The membranes contained carotenoid pigments of the bacterioruberin series and polar lipids, mostly as C20,C20 isoprenoid derivates: phosphatidylglyceromethylphosphate, phosphatidylglycerol, and three unidentified sulfated glycolipids of the S-DGD type. The DNA G+C content was 65.1–66.4 mol %. Phylogenetic analysis based on the 16S rRNA gene sequencing revealed that the thermotolerant neutrophilic isolate RS94 (DNA G+C content of 66.4 mol %) was most closely related to the nonpigmented moderate acidophile Halarchaeum acidiphilum MH1-52-1T (97.3%). Based on its phenotypic and genotypic characteristics, the organism was classified as a new species of the genus Halarchaeum with the proposed name Halarchaeum solikamskense sp. nov. The type strain is RS94T (= VKPM B-11282T). 相似文献
16.
A Gram-negative, non-motile, non-endospore-forming bacterial strain, designated DPSR-4T, was isolated from a tidal flat sediment on the southern coast of Korea. Strain DPSR-4T grew optimally at 25–30°C, at pH 7.0–7.5 and in the presence of 2% (w/v) NaCl. A Neighbour-Joining phylogenetic tree based
on 16S rRNA gene sequences revealed that strain DPSR-4T clustered with Salinimonas
chungwhensis BH030046T by a high bootstrap resampling value of 99.7%. Strain DPSR-4T exhibited 96.2% 16S rRNA gene sequence similarity to that of S. chungwhensis BH030046T and 93.7–96.6% sequence similarity to the sequences of type strains of Alteromonas species. Strain DPSR-4T contained Q-8 as the predominant ubiquinone and iso-C15:0 2-OH and/or C16:1
ω7c, C16:0 and C18:1
ω7c as the major fatty acids. The major polar lipids detected in strain DPSR-4T and S. chungwhensis KCTC 12239T were phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 53.4 mol%.
Differential phenotypic properties and phylogenetic distinctiveness of strain DPSR-4T demonstrated that this strain is distinguishable from the sole recognized species of the genus Salinimonas, S. chungwhensis. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPSR-4T is considered to represent a novel species of the genus Salinimonas, for which the name Salinimonas lutimaris sp. nov. is proposed. The type strain is DPSR-4T (KCTC 23464T, CCUG 60743T). 相似文献
17.
Grégoire P Fardeau ML Guasco S Lagière J Cambar J Michotey V Bonin P Ollivier B 《Antonie van Leeuwenhoek》2012,101(3):595-602
A novel strictly anaerobic bacterium designated SPDX02-08T was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1–2 × 2–6 μm) were non-motile,
non sporulating and stained Gram negative. Strain SPDX02-08T grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between
0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental
sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08T completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric
measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain
SPDX02-08T required yeast extract to oxidize formate and H2 but did not grow autotrophically on H2. Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular
fatty acids of strain SPDX02-08T were iso-C15:0, C15:0, and C16:0. Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08T belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08T and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08T is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937T = JCM 16410T). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08T is HM056226. 相似文献
18.
A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83T, is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83T was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83T and Cohnella ginsengisoli DSM18997T (97.99%), whereas the similarity was below 96.0% between YN-83T and the other Cohnella species. DNA–DNA relatedness between strain YN-83T and C. ginsengisoli DSM18997T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C15:0 (44.3%), iso-C15:0 (11.3%), iso-C16:0 (18.6%) and C16:0 (7.7%). The polar lipids of strain YN-83T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83T represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83T = DSM 25424T = CGMCC 1.12047T) is proposed. 相似文献
19.
Isolate RS1T isolated from used metalworking fluid was found to be a Gram-negative, motile, and non-spore forming rod. Based on phylogenetic
analyses with 16S rRNA, isolate RS1T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C18:1ω7c (32.6%), C16:0 (25.5%), and C15:0 ISO 2OH/C16:1ω7c (14.4%). The sequence similarities of isolate RS1T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062T and 0.63 with P. pseudoalcaligenes ATCC17440T. The DNA G + C content of isolate RS1T was 62.2 mol.%. The DNA–DNA relatedness was 73.0% with P. oleovorans ATCC 8062T and 79.1% with P. pseudoalcaligenes ATCC 17440T. On the basis of morphological, biochemical, and molecular studies, isolate RS1T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA–DNA relatedness (>70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440T and P. oleovorans ATCC 8062T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062T = DSM 1045T = NCIB 6576T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1T = ATCC BAA-1494T = DSM 21016T). 相似文献
20.
Hamada M Tamura T Shibata C Yamamura H Hayakawa M Suzuki K 《Antonie van Leeuwenhoek》2012,102(2):325-333
Two novel Gram-positive actinobacteria, designated H97-3T and H83-5, were isolated from marine sediment samples and their taxonomic positions were investigated by a polyphasic approach. Both strains formed vegetative hyphae in the early phase of growth but the hyphae eventually fragmented into coccoid cells. The peptidoglycan type was found to be A4α. The predominant menaquinone was MK-9(H4), and the major fatty acids were anteiso-C15:0, anteiso-C17:0 and C16:0. The DNA G+C content was 74.0–74.9 mol %. 16S rRNA gene sequencing analysis revealed that strains H97-3T and H83-5 represented novel members of the family Cellulomonadaceae. Their nearest phylogenetic neighbours were the members of the genus Oerskovia, with a similarity of 98.3–98.4 %. However, strains H97-3T and H83-5 were distinguishable from the members of the genus Oerskovia and the other genera of the family Cellulomonadaceae in terms of chemotaxonomic characteristics and phylogenetic relationship. The result of the DNA–DNA hybridization indicated that strains H97-3T and H83-5 belonged to the same species. Therefore, strains H97-3T and H83-5 represent a novel genus and species of the family Cellulomonadaceae, for which the name Sediminihabitans luteus gen. nov., sp. nov. is proposed. The type strain of S. lutes is H97-3T (=NBRC 108568T = DSM 25478T). 相似文献