Ingestion,egestion, excretion,growth, and conversion efficiency for the mummichog, Fundulus heteroclitus (L.)

The food conversion efficiency of Fundulus heteroclitus (L.) was measured in terms of calories and nitrogen for three diets: Palaemonetes sp., Nereis sp., and Uca pugnax. Fish were allowed to feed ad libitum for 30–60 min each day. Growth, ingestion, egestion, and excretion were measured for fish fed each diet. The growth rates of Fundulus heteroclitus fed two of the single item diets of invertebrates were higher than growth rates previously estimated for mummichogs from the natural population in Canary Creek marsh. In comparison with other fish species, F. heteroclitus was found to have a higher than average assimilation efficiency ($\text{X}\text{?}\text{= 87\%}$ for all three diets) and a lower than average gross growth efficiency (≈12% for two of the diets). Metabolic costs accounted for an average of 69% of ingested energy. Excretion rates were also large, with excretion accounting for more energy than egestion in two of the three diets.     

A comparison between meals prepared from the seeds of different varieties of lupin as protein supplements to barley-based diets for growing pigs

Four experiments were conducted in order to examine the growth and voluntary feed consumption of young growing pigs fed on barley-based diets containing as the sole protein supplement lupin-seed meal (LSM) from either Lupinus angustifolius cv. Uniwhite (UW), Uniharvest (UH) or L. albus cv. Neuland (NL).Under the restricted feeding regimen of the first experiment pigs receiving diets containing either 31% or 43% of either UW or UH grew at similar rates and with similar efficiency of feed utilisation from 27.5 to 85 kg live weight. By contrast, all pigs fed on diets containing these same proportions of NL refused such large amounts of feed immediately after the diets were offered that their growth halted.In the second and third experiments the voluntary feed consumption of pigs weighing 18 to 34 kg and receiving diets containing 37 to 43% of any of three distinct lines of NL was significantly less than those of pigs receiving UW diets, to the extent in some cases that body-weight loss occurred.In the fourth experiment the growth and voluntary feed intakes of 20 to 30 kg pigs fed on a control diet containing a mixture of fishmeal and dried blood as the protein supplement were greater than those of pigs fed a UW diet, which in turn were greater than those of pigs receiving NL as the source of supplementary protein. Reduction of the alkaloid content of NL by ethanol extraction resulted in a recovery of growth and feed intake to levels similar to those supported by the UW diet.     

Combined or Individual Effects of Dietary Probiotic <Emphasis Type="Italic">Pedicoccus acidilactici</Emphasis> and Nucleotide on Growth Performance,Intestinal Microbiota,Hemato-biochemical Parameters,and Innate Immune Response in Goldfish (<Emphasis Type="Italic">Carassius auratus</Emphasis>)

This study investigated the effect of dietary supplementation of probiotic Pedicoccus acidilactici and nucleotide (separately or combined) on growth performance, intestinal microbiota, hemato-immunological parameters, and immunity response in goldfish (Carassius auratus). Fish (average weight 5–6 g) were acclimatized and divided into eight experimental diets supplemented with P. acidilactici of different concentrations (0.1, 0.2, and 0.3% diet) and nucleotides (0 and 0.5% diet) for 6 months. Fish fed with experimental diets showed significant differences in terms of final weight, weight gain, feed conversion ratio, daily growth rate, and condition factor when compared to control diet (P < 0.05). Fish fed with probiotic (0.3%) separately and combined with nucleotide (0.5%) had highest RBC and WBC when compared to other diets (P < 0.05), while the highest values for Hb and Hct as well as total protein, glucose, albumin, and globulin were observed in probiotic (0.2%) and nucleotide (0.5%) combined diet. Serum lysozyme and anti-protease activities were significantly higher in probiotic (0.1 and 0.2%) and nucleotide (0.5%) combined diets. Similarly, these two diets combined showed the highest colonization of P. acidilactici when compared to other diets. In conclusion, combined dietary probiotic and nucleotide improve the growth performance, hemato-biochemical parameters, and intestine growth in C. auratus.     

Nutritive value of melon husk in the diet of chickens.

A study was conducted to investigate the nutritive value of melon husk (MH). Results showed that MH was low in crude protein but very high in crude fibre, ash and ether extract. The elemental analysis showed that MH was also high in calcium and phosphorus content. However, when MH was incorporated into starter diets of chicks, body weight gain decreased with increase in the level of MH in the diets. Birds fed on a 10% MH diet were superior to others in body weight gain (10.45 g/bird/day). Results of feed intake and efficiency of feed utilization were also superior on 10% MH diet with values of 43.07 g/bird/day and 4.12 recorded, respectively. The dietary treatment had significant effect (p < 0.05) on the protein efficiency ratio, which decreased with increase in level of MH in the diets. However, there appeared to be no treatment effects on haematological parameters. Up to 10% MH can replace maize in the starter diet of cockerels without adverse effect on performance.     

Nutritive value of dried lactic acid fermented fish silage and soybean meal in dry diets for juvenile catfish, Clarias gariepinus (Burchell, 1822)

Dry diets containing either fish meal (C-FM) or dried fermented fish silage and soybean meal blend (1:1, ww⁻¹) (C-FS) as the sole protein source, were fed to triplicate groups of juvenile Clarias gariepinus (10.8 ± 0.3 g) at 5% body weight per day for 70 days. Catfish fed the C-FS diet showed reduced (P < 0.05) growth rate, feed conversion, protein efficiency and digestibility. Lower amounts of available amino acids in the C-FS diet resulted in inferior nutritive value for catfish growth than in the C-FM diet. Postprandial changes in plasma amino acids showed similar patterns in both diet treatments, but the maximal mean levels attained for the C-FS diet were correspondingly lower and occurred earlier than with the C-FM diet. There were no effects of feeding C-FS diet on the hepatosomatic index but carcass analysis showed that body protein deposition was lower (P < 0.05). Differences in haematocrit, haemoglobin content and liver histology were demonstrated but were not pathological. Lower digestible energy of C-FS diet also contributed to the poor performance of catfish in this treatment. Results of this study indicate that C. gariepinus cannot metabolize protein from co-dried fish silage as efficiently as fish meal protein when used as the sole dietary protein.     

The nutritive value of liquid whey,either sour or sweet,when given in restricted amounts to the growing pig

Three experiments were completed in which liquid cheese whey, either sour or sweet (preserved with 0.1% formalin), was fed in restricted amounts as a partial replacement of the barley meal component of growing pig diets from 20 to 90 kg liveweight.All diets were compounded so as to provide comparable daily intakes of total protein, major minerals and vitamins and included a high-copper supplement.The performance and carcass quality of pigs fed whey in amounts to replace, on a DM basis, 30% of barley meal were closely comparable to those of all-meal fed control pigs. There were no differences of significance between pigs fed sour or sweet whey (mean titratable acidity 2.2 and 0.27, respectively), nor between pigs receiving white-fish meal or soyabean meal as the sole protein supplement in the basal meal fed with the whey.At this level of intake, it was calculated that 1.3 l whey have the same nutritive value as a source of protein and energy as 0.1 kg of barley meal (88% DM). (1 gal. whey is equivalent to $\text{3}\text{4}\text{lb}$ barley meal.)When the amount of sour whey fed replaced 50% of barley meal, there was a significant adverse effect on the performance of the pigs.Feeding with whey increased the amounts of copper found in the kidney and liver at slaughter but these did not approach toxic levels; the extent of the increase appeared to be positively correlated with the quantity and acidity of the whey. Copper stores in these two organs were reduced when soyabean meal replaced white-fish meal as the protein supplement.     

Effects of dietary sulfur amino acids and crude protein on the performance of finishing broilers

The effects of different combinations of dietary methionine+cystine (Met+Cys) and dietary crude protein (CP) in finishing broilers were investigated in two growth studies. In Exp. 1, male broilers 29 to 42 days of age were fed 18 diets containing 16.9%, 18.7%, or 20.4% CP with six levels of Met+Cys within each protein level. Similarly, in Exp. 2 another 12 diets containing either 18.0 or 21.5% CP were fed to male broilers 29 to 48 days of age.

In general, the Met+Cys requirement for optimum feed conversion was higher than for maximum growth. In both experiments, between 0.80% and 0.85% methionine+cystine optimized feed conversion. These dietary levels were valid for a 13.05 MJ ME/kg diet fed to broilers growing from 1.2 kg to 2.2 kg (Exp. 1), or for a 13.60 MJ ME/kg diet fed to broilers growing from 1.3 kg to 3.0 kg (Exp. 2).

The Met+Cys requirement was not consistently affected by the dietary CP content in Exp. 1. In Exp. 2, increasing dietary CP from 18.0% to 21.5% tended to increase the Met+Cys requirement for optimum feed conversion.

A dietary CP level of 18.0% to 18.7% supported performance and carcass fat deposition equal to diets containing 20.4% or 21.5% CP, respectively, when the sulfur amino acid content was adequate.     

Nutritional evaluation of fermented black gram (Phaseolus mungo) seed meal in compound diets for rohu, Labeo rohita (Hamilton), fingerlings

Six isonitrogenous (approximately 35% crude protein) and isocaloric (approximately 4.0 kcal g⁻¹) diets were formulated incorporating raw and fermented black gram, Phaseolus mungo, seed meal at 20%, 30% and 40% levels by weight into a fishmeal‐based control diet fed to rohu, Labeo rohita, fingerlings (mean weight, 1.81 ± 0.21 g) for 80 days for a study of fish performance. A particular bacterial strain (Bacillus sp.) isolated from the intestine of adult common carp (Cyprinus carpio) reared in the wild having significant amylolytic, cellulolytic, lipolytic and proteolytic activities was used for fermentation of seed meal for 15 days at 37 ± 2°C. Fermentation of P. mungo seed meal was effective in significantly reducing the crude fibre content and antinutritional factors such as tannins and phytic acid, and enhancing available free amino acids and fatty acids. In terms of growth, feed conversion ratio and protein efficiency ratio, the 30% fermented black gram seed meal incorporated diet resulted in a significantly (P < 0.05) better performance of rohu fingerlings. In general, growth and feed utilization efficiencies of diets containing fermented seed meal were superior to diets containing raw seed meal. The apparent protein digestibility (APD) values decreased with increasing levels of raw seed meal in the diets. The APD for raw seed meal was lower at all levels of inclusion in comparison to those for the fermented seed meals. The maximum deposition of protein in the carcass was recorded in fish fed the diet containing 40% fermented seed meal. The results indicate that fermented black gram seed meal can be incorporated in carp diets up to the 30% level compared to the 10% level of raw seed meal.     

Trypsin inhibitor,mineral availability,and performance of broiler chickens fed on diets based on rice bran

Trypsin inhibitor in rice bran was inactivated by moist heat, but not by dry heating or treatments with 0.1% H₂SO₄, 0.1% NaOH or 0.1% NaCl. Heating rice bran could not improve its growth promotion value in the diet of broilers, which was related to the feed intake. Groundnut meal was the major protein supplement in the diets. Rice bran diets did not affect weight of pancreas (1.45–1.50 g/kg body weight). Use of ⁴⁵Ca revealed that availability of calcium on a rice bran diet was lower than on a maize-based diet. A similar effect on ⁵⁹Fe-availability was noted, although this result needs confirmation. Lack of differences in weight of thyroid glands and uptake of ¹³¹I by thyroids of birds on maize- and rice bran-based diets indicated the absence of any goitrogenic substance in the rice bran.     

Effect of water temperature on dietary protein requirement,growth and body composition of Asian catfish, Clarias batrachus fry

This study aimed to evaluate the protein requirement of Clarias batrachus fry, were estimated at two different water temperatures, 28 and 32 °C. The influence of dietary protein level and water temperature on body composition, weight gain, food and nutrient utilization were estimated. The Asian catfish, C. batrachus fry were fed four diets containing 28% (diet 1), 32% (diet 2), 36% (diet 3) and 40% (diet 4) protein levels and reared at two water temperatures 28 and 32 °C for 60 days. Fry fed with diet 3 containing 36% protein showed the highest mean final body weight at 32 °C. Final body weight was significantly (P<0.05) affected by dietary treatments and temperatures. Clarias batrachus fry raised at 28 °C had higher feed efficiency (93.20%) than the fry reared at 32 °C (87.58%) with 28% dietary protein level. Further, feed efficiency decreased with increase in dietary protein level. Higher daily protein retention (0.089%) observed at lower (0.0217 g) daily protein intake at 28 °C than 0.0283 g at 32 °C. While, optimal (0.0282 g) daily protein intake showed higher daily weight gain at 32 °C. Productive protein value (% PPV) was maximum (1.76%) at 32 °C than at 28 °C (0.76%). Final body lipid recorded higher value than initial body lipid at both the temperatures. Hepatosomatic index (HSI) observed to have been influenced (P<0.05) by diets and temperatures, while viscerosomatic index (VSI) affected (P<0.05) by only diets and not (P>0.05) by temperatures. The study concluded that the diet 3 containing 36% protein was optimal for growth of C. batrachus fry at both the temperatures.     

Diurnal variations in activity of four pyridoxal enzymes in rat liver during metabolic transition from high carbohydrate to high protein diet.

The diurnal variations in enzyme activities including tyrosine aminotransferase (TAT), ornithine decarboxylase (ODC), ornithine aminotransferase (OAT) and serine dehydratase (SDH) have been studied in rats trained to a 2 hour meal feeding schedule (″2+22″) during metabolic transition from 12.5 to 60% protein diets over a period of 21 days. Although the maximal TAT activity on the first day was slightly lower compared with other days, both TAT and ODC activities adapted rapidly to the increased dietary protein from the first day. The responses of TAT and ODC to the food were so rapid that the maximal value was observed only 4 hrs after the onset of feeding. After each feeding ODC activity decreased rapidly after 4 hours, while TAT activity declined only after 6 hours had elapsed. No clear diurnal rhythm was observed in either OAT or SDH, though OAT activity tended to decrease from the beginning of the dark period and to resume a slow adaptation after about four hours. In contrast to ODC and TAT both OAT and SDH required about 7 days to fully adapt to the high protein diet. The activities of the four enzymes were also compared after 4 groups of rats had been adapted to the ″2+22″ feeding of 12.5, 30 and 60% protein diets and to 60% diet, $\text{ad}$$\text{libitum}$, respectively. The enzyme activities were not directly proportional to the protein content of the diets although higher activity was observed on the high protein diets. The diurnal variations in both TAT and ODC were observed in all ″2+22″ groups although the timing of the peak values were slightly different from each other. The maximal activities of TAT were found at earlier times in 12.5 and 30% protein groups than in the 60% protein group. The peak time for ODC activity was found at a later time in the 12.5% protein group than in rats fed 30% and 60% protein. $\text{Ad}$$\text{libitum}$ rats fed 60% protein maintained relatively high levels of TAT activity compared to the rats on the schedule. However, the maximal activity of ODC on the 60% ″2+22″ protein diet $\text{ad}$$\text{libitum}$ was so low that a diurnal rhythm was not clearly evident.     

Use of alternative protein sources for fishmeal replacement in the diet of largemouth bass (Micropterus salmoides). Part I: effects of poultry by-product meal and soybean meal on growth,feed utilization,and health

Five isonitrogenous and isocaloric diets [containing 54, 30, 15, 10, and 5% fishmeal crude-protein (CP), dry matter (DM) basis] were prepared by replacing fishmeal with poultry by-product meal plus soybean meal to feed juvenile largemouth bass (LMB, with an initial mean body weight of 4.9 g) for 8 weeks. All diets contained 54% CP and 13% lipids. There were four tanks of fish per treatment group (15 fish/tank). The fish were fed twice daily with the same feed intake (g/fish) in all the dietary groups. Results indicated that the inclusion of 15% fishmeal protein in the diet is sufficient for LMB growth. However, some of the fish that were fed diets containing ≤ 15% fishmeal CP had black skin syndrome (characterized by skin darkening and retinal degeneration, as well as intestinal and liver atrophies and structural abnormalities). The concentrations of taurine, methionine, threonine and histidine in serum were reduced (P < 0.05) in fish fed the diets containing 5, 10 and 15% fishmeal CP, compared with the 30 and 54% fishmeal CP diets. Interestingly, the concentrations of tyrosine and tryptophan in serum were higher in fish fed diets with ≤ 15% fishmeal CP than those in the 54% fishmeal CP group. These results indicated that 15% fishmeal CP in the diet containing poultry by-product meal and soybean meal was sufficient for the maximum growth and feed efficiency in LMB but inadequate for their intestinal, skin, eye, and liver health. A reduction in dietary methionine and taurine content and the possible presence of antinutritional factors in the fishmeal replacements diets containing high inclusion levels of soybean meal may contribute to black skin syndrome in LMB. We recommend that the diets of juvenile LMB contain 30% fishmeal CP (DM basis).

     

The red alga Porphyra dioica as a fish-feed ingredient for rainbow trout (Oncorhynchus mykiss): effects on growth, feed efficiency, and carcass composition

Porphyra dioica meal was added at levels of 5, 10 and 15% to a diet for rainbow trout formulated to be isonitrogenous and isolipidic. The control diet was a commercial trout diet without seaweed meal. The experimental groups were fed in triplicate for 12.5 weeks, during which fish weight increased on average from 107–261 g. Seaweed meal inclusion did not affect significantly weight gain (WG), specific growth rate (SGR), feed efficiency (FE), protein efficiency ratio (PER) and apparent digestibility coefficient of the dry matter (ADC_dm) for any of the diets. Voluntary feed intake (VFI) increased for all seaweed diets compared to the control diet but not significantly (P?>?0.05). Final weight (FW) was significantly smaller for the 15% P. dioica inclusion and hepatosomatic index (HSI) for the 10% and 15% inclusion. Carcass protein content increased for all three experimental diets, and was significantly higher for the diet with 10% seaweed inclusion. Rainbow trout fed with Porphyra meal presented a dark orange pigmentation of the flesh at the end of the trial, compared to the whitish color from the control fish. These results suggest that P. dioica can effectively be included in diets for rainbow trout up to 10% without significant negative effects on weight gain and growth performance. The pigmentation effect of the fish flesh by adding P. dioica meal to the feed is of a considerable interest to the organic salmon-farming industry.     

Growth,parasitic infection and hematology in Colossoma macropomum Cuvier, 1818 fed diets containing Allium sativum

The aim of this study was to investigate the effects of a dietary Allium sativum (garlic) addition on growth, parasite infestation and blood parameters in Colossoma macropomum (tambaqui) using 12 net‐cages (1 m³). There were four treatments with three replicates each (15 fish per replicate). Tambaqui of 112.4 g mean weight were fed for 45 days with diets containing 0, 15, 30 and 45 g of fresh garlic kg^?1 feed. Garlic did not affect the tambaqui survival, growth, feed conversion, body condition, plasma glucose levels, total plasma protein levels or red blood cells. Total leukocyte and lymphocyte numbers decreased in fish fed diets containing 45 g garlic kg^?1 feed, but neutrophils decreased in fish fed diets containing 15, 30 and 45 g garlic kg^?1 diet, while the number of immature leukocytes increased in fish fed 30 g and 45 g garlic kg^?1diet. Gills in fish fed 30 and 45 g kg^?1 diet had lower infestation (52% and 63%, respectively) of Anacanthorus spathulatus (Monogenea) compared to controls. The application of garlic in diets improved the response of leukocytes and enhanced the resistance to infections by monogeneans.     

Effect of cellulase,phytase and pectinase supplementation on growth performance and nutrient digestibility of rainbow trout (Oncorhynchus mykiss,Walbaum 1792) fry fed diets containing canola meal

This experiment was conducted to evaluate the effects of supplementing exogenous enzymes on growth, feed conversion ratio (FCR) and apparent nutrient digestibility in rainbow trout (Oncorhynchus mykiss) fry diets containing 32% canola meal. Five experimental diets (including a control diet containing no enzymes) were prepared as isonitrogenous (44% crude protein) and isocaloric (4000 kcal DE kg¹). The four other diets contained either cellulase, phytase, pectinase or an enzyme mix (a mixture of cellulase, phytase and pectinase in the same ratio). The feeding trial was conducted in triplicate for 12 weeks in 15 tanks (100‐L). At the beginning of the experiment 20 rainbow trout fry (initial weight 1.23 g) were stocked into each tank. Mean water temperature in the rearing tanks was 11°C and water flow in each tank was 6 L min^?1. At the end of the experiment the growth parameters and FCR displayed no significant differences in enzyme supplementation (P > 0.05). In addition, no differences were observed in dry matter, protein, or lipid digestibility with enzyme supplementation (P > 0.05). The results of this study showed that the addition of pectinase, phytase, cellulase or an enzyme mix to a diet containing 32% canola meal had no effect on growth, feed efficiency or dry matter, protein, or lipid digestibility in rainbow trout fry.     

The effect of supplementing pony diets with yeast on 1. In vivo and in vitro digestibility,faecal pH and particle size

Fibre is essential to maintain healthy gut; however, energy demands of performance horses can be too high to be met by forages alone. Yeast may support the function of cellulolytic bacteria to digest fibre. The aim of this work was to determine the effect of an oral supplement (VistaEQ) containing 4% live yeast on the in vitro and in vivo digestibility of high-starch (HS) and high-fibre diets (HF). Eight ponies were used in a 4 × 4 Latin square design consisting of 4- × 19-day periods and four diets: HF, HF + yeast (HFY), HS and HS + yeast (HSY). In vivo apparent digestibility (AD) was estimated using total collection technique, and faecal particle size was measured using NASCO digestive analyser. Faeces from the ponies were subsequently used as an inoculum in ANKOM RF gas production system to assess fermentation kinetics in vitro. Each module contained 1 g of feed substrate DM in the following combinations: 50% grass hay and 50% alfalfa (HF_50 : 50) or concentrate (HS_50 : 50), and 75% grass hay and 25% alfalfa (HF_75 : 25) or concentrate (HS_75 : 25) with or without yeast. Yeast was able to induce more gas production from HF_75 : 25, HS_75 : 25 and HF_50 : 50 feed substrates incubated with respective faecal inoculum base. Yeast did not affect pH in vitro when the substrates were incubated in 50 : 50 ratio, while the pH was higher for HF_75 : 25 incubated with correspondent faecal inoculum compared to HS_75 : 25 and HSY_75 : 25. Yeast had no effects on ADF and CP AD of either diet. Yeast addition increased DM (HF: 0.2%, HS: 0.4%), organic matter (HF: 0.7%, HS: 1.3%), NDF (HF: 0.5%, HS: 1.5%), total detergent fibre (HF: 0.7%; HS: 0.4%) (P < 0.05) and also tended to increase hemicellulose AD (HF: 0.9%, HS: 1.2%) (P < 0.10). Faecal pH in vivo was higher for both HF diets compared to HS diet without yeast supplementation (P < 0.001, HF and HFY: 6.8; HS: 6.6, HSY: 6.7). However, no difference was observed in faecal pH when HSY was compared to both HF diets. Yeast had no effect on the size of the faecal particles (P > 0.05). Yeast increased in vitro gas production, suggesting more energy could be extracted from the feed, and the in vivo AD of some of the nutrients when HF and HS diets were fed.     

Effects of a purine inhibitor,allopurinol, on urate metabolism in the german cockroach,Blattella germanica L. (Dictyoptera: Blattellidae)

• 1.1. Adult male and female cockroaches (Blattella germanica) were maintained on a positive nitrogen balance diet (66% protein) containing various levels of allopurinol (0–3%) to determine the effects of allopurinol on urate synthesis and storage.
• 2.2. Each insect was injected with [¹⁴C]hypoxanthine and after 1 week was analyzed for whole-body hypoxanthine, xanthine and urate radiolabel.
• 3.3. There was a general trend of decreased whole-body radiolabel retention, radiolabeled body urates and total-body urate content in both sexes with increasing amounts of dietary allopurinol.
• 4.4. Virgin female adults were allowed to feed on diets containing 0, 25 and 66% protein plus 0.1% allopurinol and were injected with [¹⁴C]xanthine.
• 5.5. After 1 week radiolabel content in the whole-body xanthine and urate pools was determined.
• 6.6. Females on the 0% protein diets contained less radiolabel in the whole-body and body urates than those on either 25 or 66% protein diets.

     

Effects of the usage of dried brewing yeast in the diets on the performance, egg traits and blood parameters in quails

This experiment was carried out to determine the effects of the usage of dried brewing yeast in quail diets on laying performance, egg traits and blood parameters. A total of 240 Japanese quails (Coturnix coturnix japonica) aged 10 weeks were randomly allocated into one control group and three treatment groups. Each group was divided into five replicates as subgroups, comprising 12 quails each. Dried brewing yeast (Saccharomyces cerevisiae) was used at the levels of 1.5%, 3.0% and 4.5% in the diets of the first, second and third treatment groups, respectively. Soyabean meal was replaced with dried brewing yeast. The diets were formulated to be isocaloric and isonitrogenous. The experimental period lasted 18 weeks. Dietary treatments did not significantly affect body weight, daily feed intake, daily protein intake, egg production, egg weight, feed efficiency, mortality, egg shell thickness, egg albumen index, egg yolk index, egg Haugh unit, the percentages of egg shell, albumen and yolk, excreta moisture and small intestinal pH. Inclusion of 3% and 4.5% dried brewing yeast in diets reduced egg yolk cholesterol concentration as mg per yolk and mg per g yolk (P < 0.01). Blood serum cholesterol of groups fed diets with dried brewing yeast was significantly lower (P < 0.01) than that of the control group. Feeding diets containing 3.0% and 4.5% dried brewing yeast resulted in significant increases (P < 0.01) in blood serum levels of total protein, alanine aminotransferase at the end of the experiment. Blood serum levels of uric acid, triglyceride, aspartate aminotransferase and alkaline phosphatase were not affected by dietary dried brewing yeast. It is concluded that dried brewing yeast can be used up to 4.5% in the diets of laying quails without adverse effects on the measured parameters.     

Distribution of Selenium in Bovine Milk and Selenium Deficiency in Rats Fed Casein-based Diets,Monitored by Lipid Peroxide Level and Glutathione Peroxidase Activity

A major proportion of selenium in bovine milk was found in fluorometric analysis to be associated with the casein fraction, largely in alkali-labile form, and the rest with the whey fraction mostly in free selenite form. This uneven distribution of milk selenium seems to provide an explanation for selenium deficiency in purified caseins. The activity of glutathione peroxidase, a selenoprotein, in the liver of growing male rats fed ad libitum low-selenium diets containing either vitamin-free casein or Torula yeast 0.065 ± 0.012 or 0.015 ± 0.004 μ g Se/g diet, respectively) for 3 weeks decreased to 4 to 6% of that of the control rats fed a commercial stock diet (0.185 ± 0.092 μ g Se/g diet). Selenium status was evaluated by three different parameters for the rats assigned under pair-feeding regimen to those vitamin-free casein-based diets which were supplemented with graded levels of selenium as sodium selenite. The hepatic levels of the thiobarbituric acid-reactive substance, an indication of lipid peroxidation, decreased to control level with selenium supplementation per g diet of 0.1 μ g and over. The hepatic glutathione peroxidase activity reached a plateau above a 0.1 μ g/g diet of selenium supplementation, whereas the erythrocyte enzyme activity increased with increasing levels of supplementary selenium. These results support the notion that semi-purified diets containing vitamin-free casein as a prime protein source would not satisfy the selenium requirement of growing animals unless deliberately supplemented with additional selenium.     

Effects of sodium chloride and sodium bicarbonate in the diet on the performance of calves

Sixty male Friesian calves were weaned from milk at 5 weeks of age. From 3 to 11 weeks of age, the calves were offered a diet of barley, meat meal, urea and 15% oat straw, supplemented with sodium chloride (NaCl) or sodium bicarbonate (NaHCO₃). The sodium (Na) contents of the diets supplemented with NaCl were 0.3, 1.1, 1.9 and 2.8%. The sodium contents of the diets supplemented with NaHCO₃ were 1.1 and 1.9%.The performance of the calves fed on the diets containing 0.3, 1.1 and 1.9% Na from NaCl was similar, but the organic matter intake and weight gains of the calves fed on the diet containing 2.8% Na were significantly lower between 5 and 11 weeks of age. The feed intake of the calves fed on the diets containing 1.1 and 1.9% Na from NaHCO₃ was 8 and 15% greater than the feed intake of the calves fed on the diet containing 0.3% Na. However, there was no significant difference in the intake of organic matter, the efficiency of feed conversion ratios or the weight gains of the calves.The sodium treatments resulted in no significant differences in the concentration of volatile fatty acids in the rumen. The addition of NaHCO₃ to the diets altered in acid—base balance in jugular blood.