6. Effect of OC on the TNF‐α‐induced DNA binding activities of MMP‐9, NF‐κB, and AP‐1 motif in HASMC. Cells were pretreated with indicated OC for 40 min in serum‐free medium, were incubated with TNF‐α (100 ng/ml) for 24 h. Nuclear extracts were analyzed by EMSA for the activated NF‐κB (A) and AP‐1 (B) using radiolabeled oligonucleotide probes, respectively. Indicated values are means of three triplicate experiments. 相似文献
The feeding behaviour of Hirschmanniella oryzae, H. imamuri, and H. spinicaudata on excised rice roots was very similar to that of other tylenchid nematodes on other hosts. Juveniles and adults of all three species invaded rice roots, causing extensive mechanical damage. Feeding and burrowing activities predisposed roots to secondary infection in unsterile cultures. Surface-sterilized nematodes did not induce necrosis in axenic cultures. 相似文献