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1.
Chia-Wei Chu Tein-Shun Tsai Inn-Ho Tsai Yao-Sung Lin Ming-Chung Tu 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2009,152(4):579-585
It has been a common belief that snake venom may help in the digestion of its prey, although direct examples and supporting evidence have not been sufficient. To address this, the present study examined whether pre-injecting natural amounts of pit viper venom into experimental mice may accelerate their digestion by the snakes or gain energy benefit as compared to the control without the envenomation. Live adults of two Asian pit viper species Trimeresurus gracilis and T. stejnegeri stejnegeri, which inhabit the cold and warm environment respectively, were the subjects studied herein. A natural dose of 1.2 mg of each of the pit viper venom in phosphate-buffered saline (PBS) was injected into the mouse (about 10% of the snake mass) before it was being fed to the same species of vipers, while the pit vipers in control group were given mouse injected with sterile PBS. The snakes were kept at 14 °C or 24 °C, and parameters of gut passage time, costs of digestion, and/or digestive efficiency were measured. The results did not support the hypotheses that envenomation facilitates prey digestion. The venom in fact caused longer first defecation time and lower assimilation energy at 14 °C. Besides, the time to reach the oxygen consumption peak, and the first defecation time of T. s. stejnegeri were longer than that of T. gracilis. 相似文献
2.
Responses of rattlesnakes to envenomated mice were examined in five experiments. In experiment 1 rattlesnakes (Crotalus durissus terrificus and C. viridis viridis) discriminated mice that they had envenomated, as indexed by number of tongue flicks and accumulated investigation time, when these prey items were paired with control mice killed by the experimenter. Experiment 2 demonstrated that rattlesnakes also exhibited this discrimination when presented with mice envenomated by a conspecific and controls killed by the experimenter. In experiments 1 and 2, we observed that rattlesnakes delivered a large number of tongue flicks to exudates associated with nasal-oral tissues of envenomated mice. It has commonly been noted that rattlesnakes typically swallow envenomated, dead mice head first. In experiment 3 this observation was statistically verified. Evidence obtained in experiment 4 indicated that nasal-oral tissues of envenomated, dead mice were discriminated from anogenital tissues by rattlesnakes. Cues arising from the nasal-oral tissues probably (1) assisted the snakes in locating the head-end of the rodent, and (2) released the swallowing modal-action pattern, the final phase of the predatory sequence. In experiment 5, rattlesnakes exhibited no discrimination between nasal-oral and anogenital tissues of non-envenomated, dead mice, indicating that the results of experiment 4 were probably dependent upon effects of envenomation. 相似文献
3.
Ho PL Serrano SM Chudzinski-Tavassi AM Moura da Silva AM Mentele R Caldas C Oliva ML Batista IF Oliveira ML 《Biochemical and biophysical research communications》2002,294(4):879-885
Angiostatin is a plasminogen-derived anti-angiogenic factor composed of its first four kringle structures. This molecule is generated by proteolytic cleavage of plasminogen by some proteolytic enzymes in vitro. Since venoms of viper snakes are a rich source of both serine- and metalloproteinase, we hypothesized that angiostatin-like polypeptides could be generated during the envenomation after snake bites and play a pathophysiological role in the local tissue damage and regeneration. Our results showed that crude venoms from several species of Bothrops snakes were able to generate angiostatin-like polypeptides and purified metalloproteinases but not serine proteinases from Bothrops jararaca and Bothrops moojeni venoms were responsible for their generation in vitro. The putative plasminogen cleavage sites by the crude venoms and purified proteinases were determined by N-terminal amino acid sequencing of the angiostatin-like molecules. Angiostatin-like peptides derived from human plasminogen digestion by jararhagin, a metalloproteinase isolated from B. jararaca venom, inhibited endothelial cell proliferation in vitro. These results indicate that angiostatin-like molecules can be generated upon snakebite envenomations and may account for the poor and incomplete regenerative response observed in the damaged tissue. 相似文献
4.
We measured oxygen consumption (Vo(2)) to estimate standard metabolic rates (SMR) in cottonmouth snakes (Agkistrodon piscivorus conanti) from Seahorse Key and the adjacent peninsula of northern Florida. The island population is unusual because adult snakes feed on fish that are dropped by colonial nesting birds, and food resources are temporally limited relative to that of mainland populations. We found no differences in SMR between island and mainland snakes at any of four experimental temperatures (15 degrees -30 degrees C), suggesting that any adjustments to energy limitations involve other aspects of physiology or behavior. As with other viperid species, the SMR of cottonmouths is about one-half of that expected from interspecific allometric regressions previously reported for snakes generally. Allometric mass exponents of SMR averaged 0.76 and were not affected by temperature. We found that Vo(2) increased with temperature (Q(10) = 2.4-2.8) and was elevated 29% during scotophase compared with photophase. Neonates exhibited elevated Vo(2)compared with older juveniles of similar size, apparently due to assimilation of yolk that is present in the neonatal gut. In adult snakes, specific dynamic action (SDA) following feeding resulted in four- to eightfold increases in Vo(2), with magnitude and duration related positively to relative meal size. The total energy devoted to SDA increased with meal size and averaged 32.8%+/-4.4% of total ingested energy. We estimate that a nonreproductive 500-g adult cottonmouth at Seahorse Key uses 3,656 kJ of assimilated energy annually for maintenance and activity, which requires ingestion of approximately 1 kg of fish. 相似文献
5.
Viperid snakes strike, envenomate, and release mammalian preyto prevent being harmed by the prey; snakes must then trackprey in the process of strike-induced chemosensory searching.Because rattlesnakes prefer to track and consume envenomatedprey, it would seem that the scent of envenomated tissue iskey to the tracking process. After striking rodents, rattlesnakesalso retain a specific chemical search image of prey items.I examined this behavioral pattern in copperheads (Agkistrodoncontortrix) from three US populations with documented dietarybiases toward mammals (Kansas), lepidopteran larvae (Texas),and amphibians (Louisiana), respectively. Experiments were conductedto assess whether copperheads form a specific search image ofnon-envenomated mouse, hornworm, and frog prey items. Additionalexperiments tested the relative importance of envenomated tissueto prey scent. Results indicate that copperheads do not forma specific search image of prey items. Preference for non-envenomatedprey items is in the order mouse > hornworm > frog forall three populations; therefore, the innate behavioral preferencefor types of prey does not match the dietary biases noted inthe literature. Envenomated mice and hornworms were preferredto all nonenvenomated prey items, but most trials involvingenvenomated frogs did not suggest envenomated prey preference.Overall, these results suggest that when the snakes search forprey, envenomated tissue stimuli are more important to snakesthan scents arising from the prey itself. Searching and consumptionbehaviors seem to be independent, suggesting that strike-inducedchemosensory searching and consumption are more complicatedbehavioral processes than previously recognized. 相似文献
6.
Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals. 相似文献
7.
Envenomation by hemotoxic enzymes continues to be a major cause of morbidity and mortality throughout the world. With regard to treatment, the gold standard to abrogate coagulopathy caused by these venoms is still the administration of antivenom; however, despite antivenom therapy, coagulopathy still occurs and recurs. Of interest, this laboratory has demonstrated in vitro and in vivo that coagulopathy inducing venom derived from snakes of the family Viperidae exposed to carbon monoxide (CO) is inhibited, potentially by an attached heme. The present investigation sought to determine if venoms derived from snakes of the Elapidae family (taipans and cobras) could also be inhibited with CO or with the metheme inducing agent, O-phenylhydroxylamine (PHA). Assessing changes in coagulation kinetics of human plasma with thrombelastography, venoms from Elapidae snakes were exposed in isolation to CO (five species) or PHA (one specie) and placed in human plasma to assess changes in procoagulant or anticoagulant activity. The procoagulant activity of two taipan venoms and anticoagulant activity of three cobra venoms were significantly inhibited by CO. The venom of the inland taipan was also inhibited by PHA. In sum, these data demonstrate indirectly that the biometal heme is likely bound to these disparate venoms as an intermediary modulatory molecule. In conclusion, CO may not just be a potential therapeutic agent to treat envenomation but also may be a potential modulator of heme as a protective mechanism for venomous snakes against injury from their own proteolytic venoms. 相似文献
8.
David Chiszar Barbara O'Connell Robin Greenlee Bela Demeter Trooper Walsh Joan Chiszar Katy Moran Hobart M. Smith 《Zoo biology》1985,4(3):291-294
Rattlesnakes typically strike and release adult rodent prey. Striking is followed by a sustained, high rate of tongue flicking that guides the snake to the envenomated, dead prey. Wild-caught rattlesnakes exhibited this chemosensory searching for about 2.5 h, and the present study demonstrated that long-term captive rattlesnakes (Crotalus atrox, C durissus, C horridus, C vegrandis, C unicolor) at three zoos did the same. Because these zoo-raised snakes had always been offered dead rodents and because the snakes had become accustomed to ingesting them without striking, the present snakes had rarely exercised their innate predatory repertoires. The duration of chemosensory searching in these snakes indicates that this important aspect of the predatory repertoire had not been degraded as a consequence of long-term captive husbandry. 相似文献
9.
Kryukova NA Dubovskiy IM Chertkova EA Vorontsova YL Slepneva IA Glupov VV 《Journal of insect physiology》2011,57(6):796-800
The cellular and humoral immune reactions in haemolymph of the wax moth Galleria mellonella larvae naturally injected by venom of ectoparasitic wasp Habrobracon hebetor were analyzed. A strong decline of phenoloxidase (PO) activity in the haemolymph and the number of haemocytes with PO activity of envenomated wax moth was observed. In addition, it has been shown that the rate of capsule melanization in the envenomated larvae was half that of the control. Also production of reactive oxygen species (ROS) in the haemolymph of envenomated larvae decreased. The obtained data casts light on the suppression of the main immune reactions in G. mellonella larvae during natural envenomation by H. hebetor. 相似文献
10.
Secor SM 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2001,128(3):565-577
Among snakes a correlation exists between feeding habits (frequent or infrequent) and the magnitude by which digestive performance is regulated (modest or large). This paper investigates whether the observed regulation of digestive performance is an adaptation to feeding habits and therefore, a product of natural selection. Using data on metabolic and intestinal responses to feeding for amphibians and reptiles, it is attempted to show the selective advantage and independent origin of either modestly or widely regulating gut performance. In an energetic model, snakes that naturally feed frequently on small meals benefit (from lower energy output) from modestly regulating gut performance as opposed to widely regulating gut performance. Likewise, the model suggests an energetic benefit for infrequently-feeding snakes secondary to the wide regulation of gut performance. This benefit is a function of long spans of fasting with a down-regulated gut (thereby incurring a lower standard metabolic rate) and the occasionally incursion of a costly up-regulation of the gut. In a comparison across several distantly-related lineages of amphibians and reptiles, frequently-feeding species all exhibit small postprandial responses in metabolism and intestinal nutrient transport capacities. In contrast, frogs and snakes that routinely fast for long periods independently experience five- to 30-fold increases in metabolism and intestinal performance with feeding. Among amphibians and reptiles the evidence presented supports the hypothesis that the extent by which the gut is regulated is an adaptive trait that evolved with divergence in feeding habits and energy budgets. In finishing, the foundations, caveats, and suggested future tests of this adaptive hypothesis are presented. 相似文献
11.
Sherin Kaul L. Sai Keerthana Pankaj Kumar Komal Birader Yathirajarao Tammineni Deepali Rawat Pankaj Suman 《PLoS neglected tropical diseases》2021,15(10)
Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1 – κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of Naja naja (p < 0.0001) and Bungarus caeruleus (p<0.0001), without showing any immunoreactivity against the viperidae snakes in big four venomous snakes. The kit developed attained the limit of quantitation of 170 pg/μL and 2.1 ng/μL in spiked buffer samples and 28.7 ng/μL and 110 ng/μL in spiked serum samples for detection of N. naja and B. caeruleus venoms, respectively. This kit holds enormous potential in identification of elapid venom of the big four snakes for effective prognosis of an envenomation; as per the existing medical guidelines. 相似文献
12.
Juliana L. Bernardoni Leijiane F. Sousa Luciana S. Wermelinger Aline S. Lopes Benedito C. Prezoto Solange M. T. Serrano Russolina B. Zingali Ana M. Moura-da-Silva 《PloS one》2014,9(10)
Snake venom metalloproteinases (SVMPs) are major components in most viperid venoms that induce disturbances in the hemostatic system and tissues of animals envenomated by snakes. These disturbances are involved in human pathology of snake bites and appear to be essential for the capture and digestion of snake''s prey and avoidance of predators. SVMPs are a versatile family of venom toxins acting on different hemostatic targets which are present in venoms in distinct structural forms. However, the reason why a large number of different SVMPs are expressed in some venoms is still unclear. In this study, we evaluated the interference of five isolated SVMPs in blood coagulation of humans, birds and small rodents. P-III class SVMPs (fractions Ic, IIb and IIc) possess gelatinolytic and hemorrhagic activities, and, of these, two also show fibrinolytic activity. P-I class SVMPs (fractions IVa and IVb) are only fibrinolytic. P-III class SVMPs reduced clotting time of human plasma. Fraction IIc was characterized as prothrombin activator and fraction Ic as factor X activator. In the absence of Ca2+, a firm clot was observed in chicken blood samples with fractions Ic, IIb and partially with fraction IIc. In contrast, without Ca2+, only fraction IIc was able to induce a firm clot in rat blood. In conclusion, functionally distinct forms of SVMPs were found in B. neuwiedi venom that affect distinct mechanisms in the coagulation system of humans, birds and small rodents. Distinct SVMPs appear to be more specialized to rat or chicken blood, strengthening the current hypothesis that toxin diversity enhances the possibilities of the snakes for hunting different prey or evading different predators. This functional diversity also impacts the complexity of human envenoming since different hemostatic mechanisms will be targeted by SVMPs accounting for the complexity of the response of humans to venoms. 相似文献
13.
Magda Hagag Anthony T. Tu Farid El-Asmar 《Archives of biochemistry and biophysics》1983,220(2):459-466
Two neurotoxins, minax toxins 1 and 2, were isolated from venom of the scorpion Buthus minax from the Sudan. Molecular weights of 7000 and 6800 and 66 and 62 amino acids were found for minax toxins 1 and 2, respectively. Both toxins contain four disulfide bonds, 1 mol each of phenylalanine, histidine, and tryptophan, no free sulfhydryl groups, and no methionine. Both minax toxins 1 and 2 are basic polypeptides with isoelectric points of 8.2 and 9.0, respectively. There is a significant increase in the calcium content of rat hearts envenomated with minax toxins 1 and 2 or crude venom. This confirms earlier electron microscopic findings of calcium deposits in the heart following scorpion envenomation. There is a concomitant decrease in the calcium and phosphorus content of rat serum following envenomation. It seems that neither scorpion toxins nor scorpion venoms affect the mineral metabolism of the bone. The present investigation indicates that scorpion toxins have not only a neurotoxic action but also broader biological effects such as mineral metabolism. 相似文献
14.
Banerjee T Valacchi G Ziboh VA van der Vliet A 《Molecular and cellular biochemistry》2002,238(1-2):105-110
The present study attempts to establish the isoforms of hyaluronidase enzyme and their possible role in the spreading of toxins during envenomation. Screening of venoms of 15 snakes belonging to three different families revealed varied hyaluronidase activity in ELISA-like assay, but with relatively similar pH and temperature optima. The zymograms of individual venoms showed varied activity banding patterns and indicated the presence of at least two molecular forms of the enzyme. During envenomation, activity of hyaluronidase is considered crucial for the spreading of toxins and is presumed to distort the integrity of extracellular matrix through the degradation of hyaluronic acid in it. This property has been addressed through localization of hyaluronic acid in human skin and muscle tissue sections using the probe, biotinylated hyaluronic acid binding protein. Faint and discontinuous staining pattern of hyaluronidase treated tissue sections over intense staining of untreated tissue sections confirm the selective degradation of hyaluronic acid in extracellular matrix and thus provide an evidence for the spreading property of the enzyme. 相似文献
15.
Snake venomics of the lancehead pitviper Bothrops asper: geographic, individual, and ontogenetic variations 总被引:1,自引:0,他引:1
Alape-Girón A Sanz L Escolano J Flores-Díaz M Madrigal M Sasa M Calvete JJ 《Journal of proteome research》2008,7(8):3556-3571
We report the comparative proteomic characterization of the venoms of adult and newborn specimens of the lancehead pitviper Bothrops asper from two geographically isolated populations from the Caribbean and the Pacific versants of Costa Rica. The crude venoms were fractionated by reverse-phase HPLC, followed by analysis of each chromatographic fraction by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. The two B. asper populations, separated since the late Miocene or early Pliocene (8-5 mya) by the Guanacaste Mountain Range, Central Mountain Range, and Talamanca Mountain Range, contain both identical and different (iso)enzymes from the PLA 2, serine proteinase, and SVMP families. Using a similarity coefficient, we estimate that the similarity of venom proteins between the two B. asper populations may be around 52%. Compositional differences between venoms among different geographic regions may be due to evolutionary environmental pressure acting on isolated populations. To investigate venom variability among specimens from the two B. asper populations, the reverse-phase HPLC protein profiles of 15 venoms from Caribbean specimens and 11 venoms from snakes from Pacific regions were compared. Within each B. asper geographic populations, all major venom protein families appeared to be subjected to individual variations. The occurrence of intraspecific individual allopatric variability highlights the concept that a species, B. asper in our case, should be considered as a group of metapopulations. Analysis of pooled venoms of neonate specimens from Caribbean and Pacific regions with those of adult snakes from the same geographical habitat revealed prominent ontogenetic changes in both geographical populations. Major ontogenetic changes appear to be a shift from a PIII-SVMP-rich to a PI-SVMP-rich venom and the secretion in adults of a distinct set of PLA 2 molecules than in the neonates. In addition, the ontogenetic venom composition shift results in increasing venom complexity, indicating that the requirement for the venom to immobilize prey and initiate digestion may change with the size (age) of the snake. Besides ecological and taxonomical implications, the geographical venom variability reported here may have an impact in the treatment of bite victims and in the selection of specimens for antivenom production. The occurrence of intraspecies variability in the biochemical composition and symptomatology after envenomation by snakes from different geographical location and age has long been appreciated by herpetologist and toxinologists, though detailed comparative proteomic analysis are scarce. Our study represents the first detailed characterization of individual and ontogenetic venom protein profile variations in two geographical isolated B. asper populations, and highlights the necessity of using pooled venoms as a statistically representative venom for antivenom production. 相似文献
16.
乌梢蛇血清的抗出血因子:一个有前途的抗蛇毒药物原料 总被引:2,自引:0,他引:2
用柱层析和聚丙烯酰胺凝胶盘状电泳法,从乌梢蛇血清中分离纯化了一个抗出血因子。用SDS-聚丙烯酰胺凝胶电泳法测得其分子量大约为65 kD;测定了五种蝮亚科蛇毒(尖吻蝮、竹叶青蛇、原矛头蝮、哈扑和短尾蝮)的最小出血剂量和乌梢蛇血清中抗出血因子对这五种蛇毒的抗出血活性;还测定了七种蛇毒(除上述五种毒蛇外,还包括圆斑蝰和银环蛇)的半数致死量,以及抗出血因子对中毒小鼠的治疗作用。结果显示:从乌梢蛇血清中提纯的抗出血因子的抗蛇毒活性,不仅可以抵抗它的捕食者尖吻蝮的蛇毒,而且还可以抵抗具出血活性的其它蛇毒;但它对不具出血活性的银环蛇毒的致死抑制作用不明显。该抗出血因子不仅在体外实验表现出强的中和出血毒素的活性,而且在体内实验中亦表现出对中毒小鼠良好的治疗作用,因而可能成为新的抗蛇毒药物的有前途的原料。乌梢蛇血清对血循毒的中和能力的获得,可能归因于尖吻蝮与乌梢蛇之间捕食与被捕食相互作用的关系。 相似文献
17.
Kavi Ratanabanangkoon Kae Yi Tan Sukanya Eursakun Choo Hock Tan Pavinee Simsiriwong Teeraporn Pamornsakda Witthawat Wiriyarat Chaiya Klinpayom Nget Hong Tan 《PLoS neglected tropical diseases》2016,10(4)
Snakebite envenomation is a serious medical problem in many tropical developing countries and was considered by WHO as a neglected tropical disease. Antivenom (AV), the rational and most effective treatment modality, is either unaffordable and/or unavailable in many affected countries. Moreover, each AV is specific to only one (monospecific) or a few (polyspecific) snake venoms. This demands that each country to prepare AV against its local snake venoms, which is often not feasible. Preparation of a ‘pan-specific’ AV against many snakes over a wide geographical area in some countries/regions has not been possible. If a ‘pan-specific’ AV effective against a variety of snakes from many countries could be prepared, it could be produced economically in large volume for use in many countries and save many lives. The aim of this study was to produce a pan-specific antiserum effective against major medically important elapids in Asia. The strategy was to use toxin fractions (TFs) of the venoms in place of crude venoms in order to reduce the number of antigens the horses were exposed to. This enabled inclusion of a greater variety of elapid venoms in the immunogen mix, thus exposing the horse immune system to a diverse repertoire of toxin epitopes, and gave rise to antiserum with wide paraspecificity against elapid venoms. Twelve venom samples from six medically important elapid snakes (4 Naja spp. and 2 Bungarus spp.) were collected from 12 regions/countries in Asia. Nine of these 12 venoms were ultra-filtered to remove high molecular weight, non-toxic and highly immunogenic proteins. The remaining 3 venoms were not ultra-filtered due to limited amounts available. The 9 toxin fractions (TFs) together with the 3 crude venoms were emulsified in complete Freund’s adjuvant and used to immunize 3 horses using a low dose, low volume, multisite immunization protocol. The horse antisera were assayed by ELISA and by in vivo lethality neutralization in mice. The findings were: a) The 9 TFs were shown to contain all of the venom toxins but were devoid of high MW proteins. When these TFs, together with the 3 crude venoms, were used as the immunogen, satisfactory ELISA antibody titers against homologous/heterologous venoms were obtained. b) The horse antiserum immunologically reacted with and neutralized the lethal effects of both the homologous and the 16 heterologous Asian/African elapid venoms tested. Thus, the use of TFs in place of crude venoms and the inclusion of a variety of elapid venoms in the immunogen mix resulted in antiserum with wide paraspecificity against elapid venoms from distant geographic areas. The antivenom prepared from this antiserum would be expected to be pan-specific and effective in treating envenomations by most elapids in many Asian countries. Due to economies of scale, the antivenom could be produced inexpensively and save many lives. This simple strategy and procedure could be readily adapted for the production of pan-specific antisera against elapids of other continents. 相似文献
18.
Gabriela D. Tanaka Maria de Fátima D. Furtado Fernanda C. V. Portaro Osvaldo Augusto Sant'Anna Denise V. Tambourgi 《PLoS neglected tropical diseases》2010,4(3)
Background
Micrurus snake bites can cause death by muscle paralysis and respiratory arrest, few hours after envenomation. The specific treatment for coral snake envenomation is the intravenous application of heterologous antivenom and, in Brazil, it is produced by horse immunization with a mixture of M. corallinus and M. frontalis venoms, snakes that inhabit the South and Southeastern regions of the country. However, this antivenom might be inefficient, considering the existence of intra- and inter-specific variations in the composition of the venoms. Therefore, the aim of the present study was to investigate the toxic properties of venoms from nine species of Micrurus: eight present in different geographic regions of Brazil (M. frontalis, M. corallinus, M. hemprichii, M. spixii, M. altirostris, M. surinamensis, M. ibiboboca, M. lemniscatus) and one (M. fulvius) with large distribution in Southeastern United States and Mexico. This study also analyzed the antigenic cross-reactivity and the neutralizing potential of the Brazilian coral snake antivenom against these Micrurus venoms.Methodology/Principal Findings
Analysis of protein composition and toxicity revealed a large diversity of venoms from the nine Micrurus species. ELISA and Western blot assays showed a varied capability of the therapeutic antivenom to recognize the diverse species venom components. In vivo and in vitro neutralization assays indicated that the antivenom is not able to fully neutralize the toxic activities of all venoms.Conclusion
These results indicate the existence of a large range of both qualitative and quantitative variations in Micrurus venoms, probably reflecting the adaptation of the snakes from this genus to vastly dissimilar habitats. The data also show that the antivenom used for human therapy in Brazil is not fully able to neutralize the main toxic activities present in the venoms from all Micrurus species occurring in the country. It suggests that modifications in the immunization scheme, with the inclusion of other venoms in the antigenic mixture, should occur in order to generate effective therapeutic coral snake antivenom. 相似文献19.
William H. Karasov Edwin Petrossian Laura Rosenberg Jared M. Diamond 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1986,156(4):599-609
Summary What digestive adaptations permit herbivorous nonruminant mammals to sustain much higher metabolic rates than herbivorous lizards, despite gross similarity in digestive anatomy and physiology? We approached this question by comparing four herbivorous species eating the same diet of alfalfa pellets: two lizards (chuckwalla and desert iugana) and two mammals (desert woodrat and laboratory mouse). The mammals had longer small and large intestines, greater intestinal surface area, much higher (by an order of magnitude) food intake normalized to metabolic live mass, and much faster food passage times (a few hours instead of a few days). Among both reptiles and mammals, passage times increase with body size and are longer for herbivores than for carnivores. The herbivorous lizards, despite these much slower passage times, had slightly lower apparent digestive efficiencies than the mammals. At least for chuckwallas, this difference from mammals was not due to differences in body temperature regime. Comparisons of chuckwallas and woodrats in their assimilation of various dietary components showed that the woodrat's main advantage lay in greater assimilation of the dietary fiber fraction. Woodrats achieved greater fiber digestion despite shorter residence time, but possibly because of a larger fermentation chamber, coprophagy, and/or different conditions for microbial fermentation. We conclude with a comparative overview of digestive function in herbivorous lizards and mammals, and with a list of four major unsolved questions. 相似文献
20.
Simulation of seed passage through a bird’s gut is an important tool for comparing the effect of bird digestion and thus the potential for plant dispersal by endozoochory. However, sufficient methodology is missing. Thus, we subjected seeds of 20 plant species to seven different simulations of gut passage and to the real passage through a pigeon’s gut to determine which simulation type best reflects the effects of real bird digestion. We also measured various seed traits to identify the traits responsible for differences between species. Results show that four out of seven simulations were significant predictors of seed survival after gut passage. The fit between direct digestion by the pigeon and the different simulation treatments was, however, species-specific and depends not only on the commonly tested traits such as seed mass and water permeability, but also on other unmeasured traits. Seed mass was the best predictor of differences between real digestion and simulation. Selecting one type of simulation to be a good predictor of seed survival after gut passage is difficult. The strongest simulation (24-h scarification and 240-min acid immersion) is the best predictor and may be used to compare the ability of seeds to be dispersed by bird endozoochory. Such knowledge can be included in databases of species traits, as is currently done for many other species traits. 相似文献