共查询到20条相似文献,搜索用时 15 毫秒
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Sheng Liu Huibin Huang Xinqi Yi Yuanyuan Zhang Qingyong Yang Chunyu Zhang Chuchuan Fan Yongming Zhou 《Plant biotechnology journal》2020,18(6):1472-1484
Glucosinolates (GSLs), whose degradation products have been shown to be increasingly important for human health and plant defence, compose important secondary metabolites found in the order Brassicales. It is highly desired to enhance pest and disease resistance by increasing the leaf GSL content while keeping the content low in seeds of Brassica napus, one of the most important oil crops worldwide. Little is known about the regulation of GSL accumulation in the leaves. We quantified the levels of 9 different GSLs and 15 related traits in the leaves of 366 accessions and found that the seed and leaf GSL content were highly correlated (r = 0.79). A total of 78 loci were associated with GSL traits, and five common and eleven tissue‐specific associated loci were related to total leaf and seed GSL content. Thirty‐six candidate genes were inferred to be involved in GSL biosynthesis. The candidate gene BnaA03g40190D (BnaA3.MYB28) was validated by DNA polymorphisms and gene expression analysis. This gene was responsible for high leaf/low seed GSL content and could explain 30.62% of the total leaf GSL variation in the low seed GSL panel and was not fixed during double‐low rapeseed breeding. Our results provide new insights into the genetic basis of GSL variation in leaves and seeds and may facilitate the metabolic engineering of GSLs and the breeding of high leaf/low seed GSL content in B. napus. 相似文献
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Ryoichi Araki Akiko Hasumi Osamu Ishizaki Nishizawa Katsunori Sasaki Ayuko Kuwahara Yuji Sawada Yasushi Totoki Atsushi Toyoda Yoshiyuki Sakaki Yimeng Li Kazuki Saito Toshiya Ogawa Masami Yokota Hirai 《Plant biotechnology journal》2013,11(8):1017-1027
Plants belonging to the Brassicaceae family exhibit species‐specific profiles of glucosinolates (GSLs), a class of defence compounds against pathogens and insects. GSLs also exhibit various human health–promoting properties. Among them, glucoraphanin (aliphatic 4‐methylsulphinylbutyl GSL) has attracted the most attention because it hydrolyses to form a potent anticancer compound. Increased interest in developing commercial varieties of Brassicaceae crops with desirable GSL profiles has led to attempts to identify genes that are potentially valuable for controlling GSL biosynthesis. However, little attention has been focused on genes of kale (Brassica oleracea var. acephala). In this study, we established full‐length kale cDNA libraries containing 59 904 clones, which were used to generate an expressed sequence tag (EST) data set with 119 204 entries. The EST data set clarified genes related to the GSL biosynthesis pathway in kale. We specifically focused on BoMYB29, a homolog of Arabidopsis MYB29/PMG2/HAG3, not only to characterize its function but also to demonstrate its usability as a biological resource. BoMYB29 overexpression in wild‐type Arabidopsis enhanced the expression of aliphatic GSL biosynthetic genes and the accumulation of aliphatic GSLs. When expressed in the myb28myb29 mutant, which exhibited no detectable aliphatic GSLs, BoMYB29 restored the expression of biosynthetic genes and aliphatic GSL accumulation. Interestingly, the ratio of methylsulphinyl GSL content, including glucoraphanin, to that of methylthio GSLs was greatly increased, indicating the suitability of BoMYB29 as a regulator for increasing methylsulphinyl GSL content. Our results indicate that these biological resources can facilitate further identification of genes useful for modifications of GSL profiles and accumulation in kale. 相似文献
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Pankaj Sharma Prabhu Dayal Meena Jitendra Singh Chauhan 《Journal of Phytopathology》2013,161(6):439-441
A stem blight disease was observed on the lower portions of Brassica juncea stems during the cropping season (2010–2011). In advanced stages, the lesions were up to 120 cm in length on the stems and also spread to petioles and midribs of leaves. The purified fungus was identified as Nigrospora oryzae (Berk. & Br.) Petch (teleomorph Khuskia oryzae), which produced similar symptoms when healthy B. juncea plants were inoculated, thus proving Koch's postulates. This is the first report of the occurrence of N. oryzae on B. juncea. 相似文献
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Valuable New Leaf or Inflorescence Resistances Ensure Improved Management of White Rust (Albugo candida) in Mustard (Brassica juncea) Crops 下载免费PDF全文
Martin J. Barbetti Cai Xia Li Ming Pei You Dhiraj Singh Abha Agnihotri Shashi K. Banga Prabhjodh S. Sandhu Rajender Singh Surinder S. Banga 《Journal of Phytopathology》2016,164(6):404-411
Field resistances/susceptibilities against Albugo candida race 2V were determined for 29 Indian Brassica juncea varieties and compared with resistant varieties from China (6) and Australia (7). ‘Basanti’ (AUDPC incidence 46.7; AUDPC severity 29.2) represents the first high‐level resistance to race 2V in Indian varieties. Several others showed lower but still useful levels of resistance, including Narendra Ageti Rai‐4 (AUDPC incidence 150.6; AUDPC severity 66.8) and JM1 (AUDPC incidence 167.1; AUDPC severity 83.7). Highly susceptible Indian varieties had AUDPC incidence values >200 and severity >100. ‘Basanti’ had least stagheads/plant (0.32), while Narendra Ageti Rai‐4 had lowest % plants with stagheads (2.48). In contrast, almost half of Indian varieties had stagheads/plant >1 and % plants with stagheads >4, and >26 for ‘Kranti’. The resistance in ‘Basanti’ paves the way forward towards significantly improved white rust management in mustard in India. JM06011, JM06021, JR049 from Australia and CJB‐003 from China had zero leaf incidence. There were significant (P < 0.001) relationships between disease incidence with severity (R2 0.92), stagheads/plant (R2 0.69) and also % plants with stagheads (R2 0.60); between disease severity with stagheads/plant (R2 0.68) and also % plants with stagheads (R2 0.69); and between stagheads/plant with % plants with stagheads (R2 0.59). 相似文献
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Valuable New Resistances Ensure Improved Management of Sclerotinia Stem Rot (Sclerotinia sclerotiorum) in Horticultural and Oilseed Brassica Species 下载免费PDF全文
Ming Pei You Margaret B. Uloth Xi Xiang Li Surinder S. Banga Shashi K. Banga Martin J. Barbetti 《Journal of Phytopathology》2016,164(5):291-299
Field resistances against Sclerotinia rot (SR) (Sclerotinia sclerotiorum) were determined in 52 Chinese genotypes of Brassica oleracea var. capitata, 14 Indian Brassica juncea genotypes carrying wild weedy Brassicaceae introgression(s) and four carrying B‐genome introgression, 22 Australian commercial Brassica napus varieties, and 12 B. napus and B. juncea genotypes of known resistance. All plants were individually inoculated by securing an agar disc from a culture of S. sclerotiorum growing on a glucose‐rich medium to the stem above the second internode with Parafilm tape. Mean stem lesion length across tested genotypes ranged from <1 to >68 mm. While there was considerable diversity within the germplasm sets from each country, overall, 65% of the B. oleracea var. capitata genotypes from China showed the highest levels of stem resistance, a level comparable with the highest resistance ever recorded for oilseed B. napus or B. juncea from China or Australia. One Indian B. juncea line carrying weedy introgression displayed a significant level of both stem and leaf resistance. However, the vast majority of commercial Australian oilseed B. napus varieties fell within the most susceptible 40% of genotypes tested for stem disease. There was no correlation between expressions of stem versus leaf resistance, suggesting their independent inheritance. A few Chinese B. oleracea var. capitata genotypes that expressed combined extremely high‐level stem (≤1 mm length) and leaf (≤0.5 mean number of infections/plant) resistance will be particularly significant for developing new SR‐resistant horticultural and oilseed Brassica varieties. 相似文献
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Expression of the MYB transcription factor gene BplMYB46 affects abiotic stress tolerance and secondary cell wall deposition in Betula platyphylla 总被引:1,自引:0,他引:1 下载免费PDF全文
Huiyan Guo Yucheng Wang Liuqiang Wang Ping Hu Yanmin Wang Yuanyuan Jia Chunrui Zhang Yu Zhang Yiming Zhang Chao Wang Chuanping Yang 《Plant biotechnology journal》2017,15(1):107-121
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Host‐induced gene silencing of an essential chitin synthase gene confers durable resistance to Fusarium head blight and seedling blight in wheat 下载免费PDF全文
Wei Cheng Xiu‐Shi Song He‐Ping Li Le‐Hui Cao Ke Sun Xiao‐Li Qiu Yu‐Bin Xu Peng Yang Tao Huang Jing‐Bo Zhang Bo Qu Yu‐Cai Liao 《Plant biotechnology journal》2015,13(9):1335-1345
Fusarium head blight (FHB) and Fusarium seedling blight (FSB) of wheat, caused by Fusarium pathogens, are devastating diseases worldwide. We report the expression of RNA interference (RNAi) sequences derived from an essential Fusarium graminearum (Fg) virulence gene, chitin synthase (Chs) 3b, as a method to enhance resistance of wheat plants to fungal pathogens. Deletion of Chs3b was lethal to Fg; disruption of the other Chs gene family members generated knockout mutants with diverse impacts on Fg. Comparative expression analyses revealed that among the Chs gene family members, Chs3b had the highest expression levels during Fg colonization of wheat. Three hairpin RNAi constructs corresponding to the different regions of Chs3b were found to silence Chs3b in transgenic Fg strains. Co‐expression of these three RNAi constructs in two independent elite wheat cultivar transgenic lines conferred high levels of stable, consistent resistance (combined type I and II resistance) to both FHB and FSB throughout the T3 to T5 generations. Confocal microscopy revealed profoundly restricted mycelia in Fg‐infected transgenic wheat plants. Presence of the three specific short interfering RNAs in transgenic wheat plants was confirmed by Northern blotting, and these RNAs efficiently down‐regulated Chs3b in the colonizing Fusarium pathogens on wheat seedlings and spikes. Our results demonstrate that host‐induced gene silencing of an essential fungal chitin synthase gene is an effective strategy for enhancing resistance in crop plants under field test conditions. 相似文献
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We report in this study, the successful deployment of a double mutant acetolactate synthase gene (ALSdm, containing Pro 197 to Ser and Ser 653 to Asn substitutions) as an efficient in vitro selection marker for the development of transgenic plants in Brassica juncea (oilseed mustard). The ALS enzyme is inhibited by two categories of herbicides, sulfonylureas (e.g. chlorsulfuron) and imidazolinones (e.g. imazethapyr), while the mutant forms are resistant to the same. Three different selection agents (kanamycin, chlorsulfuron and imazethapyr) were tested for in vitro selection efficiency in two B. juncea cultivars, RLM198 and Varuna. For both the cultivars, higher transformation frequencies were obtained using chlorsulfuron (3.8 +/- 0.6% and 4.6 +/- 0.9% for RLM198 and Varuna, respectively) and imazethapyr (10.2 +/- 0.7% for RLM198 and 7.8 +/- 1.2% for Varuna) as compared to that obtained on kanamycin (3.1 +/- 0.2% and 2.8 +/- 0.5% for RLM198 and Varuna, respectively). Additionally, transformation frequencies were higher on imazethapyr than on chlorsulfuron for both the cultivars indicating that imidazolinones are better selective agents than sulfonylureas for the selection of mustard transgenics. 相似文献
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Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants 下载免费PDF全文
Yang Yang Ren‐Jie Tang Chun‐Mei Jiang Bei Li Tao Kang Hua Liu Nan Zhao Xu‐Jun Ma Lei Yang Shao‐Liang Chen Hong‐Xia Zhang 《Plant biotechnology journal》2015,13(7):962-973
In higher plants, the salt overly sensitive (SOS) signalling pathway plays a crucial role in maintaining ion homoeostasis and conferring salt tolerance under salinity condition. Previously, we functionally characterized the conserved SOS pathway in the woody plant Populus trichocarpa. In this study, we demonstrate that overexpression of the constitutively active form of PtSOS2 (PtSOS2TD), one of the key components of this pathway, significantly increased salt tolerance in aspen hybrid clone Shanxin Yang (Populus davidiana × Populus bolleana). Compared to the wild‐type control, transgenic plants constitutively expressing PtSOS2TD exhibited more vigorous growth and produced greater biomass in the presence of high concentrations of NaCl. The improved salt tolerance was associated with a decreased Na+ accumulation in the leaves of transgenic plants. Further analyses revealed that plasma membrane Na+/H+ exchange activity and Na+ efflux in transgenic plants were significantly higher than those in the wild‐type plants. Moreover, transgenic plants showed improved capacity in scavenging reactive oxygen species (ROS) generated by salt stress. Taken together, our results suggest that PtSOS2 could serve as an ideal target gene to genetically engineer salt‐tolerant trees. 相似文献
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Sue Lin Xiaoyan Yue Yingjing Miao Youjian Yu Heng Dong Li Huang Jiashu Cao 《The Plant journal : for cell and molecular biology》2018,94(1):60-76
Arabinogalactan proteins (AGPs) are extensively glycosylated hydroxyproline‐rich glycoproteins ubiquitous in all plant tissues and cells. AtAGP6 and AtAGP11, the only two functionally known pollen‐specific classical AGP encoding genes in Arabidopsis, are reported to have redundant functions in microspore development. BcMF18 and BcMF8 isolated from Brassica campestris are the orthologues of AtAGP6 and AtAGP11, respectively. In contrast to the functional redundancy of AtAGP6 and AtAGP11, single‐gene disruption of BcMF8 led to deformed pollen grains with abnormal intine development and ectopic aperture formation in B. campestris. Here, we further explored the action of BcMF18 and its relationship with BcMF8. BcMF18 was specifically expressed in pollen during the late stages of microspore development. Antisense RNA transgenic lines with BcMF18 reduction resulted in aberrant pollen grains with abnormal cellulose distribution, lacking intine, cytoplasm and nuclei. Transgenic plants with repressive expression of both BcMF8 and BcMF18 showed a hybrid phenotype, expressing a mixture of the phenotypes of the single gene knockdown plant lines. In addition, we identified functional diversity between BcMF18/BcMF8 and AtAGP6/AtAGP11, mainly reflected by the specific contribution of BcMF18 and BcMF8 to pollen wall formation. These results suggest that, unlike the orthologous genes AtAGP6 and AtAGP11 in Arabidopsis, BcMF18 and BcMF8 are both integral to pollen biogenesis in B. campestris, acting through independent pathways during microspore development. 相似文献
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Homoeologous exchange is a major cause of gene presence/absence variation in the amphidiploid Brassica napus 下载免费PDF全文
Bhavna Hurgobin Agnieszka A. Golicz Philipp E. Bayer Chon‐Kit Kenneth Chan Soodeh Tirnaz Aria Dolatabadian Sarah V. Schiessl Birgit Samans Juan D. Montenegro Isobel A. P. Parkin J. Chris Pires Boulos Chalhoub Graham J. King Rod Snowdon Jacqueline Batley David Edwards 《Plant biotechnology journal》2018,16(7):1265-1274
Homoeologous exchanges (HEs) have been shown to generate novel gene combinations and phenotypes in a range of polyploid species. Gene presence/absence variation (PAV) is also a major contributor to genetic diversity. In this study, we show that there is an association between these two events, particularly in recent Brassica napus synthetic accessions, and that these represent a novel source of genetic diversity, which can be captured for the improvement of this important crop species. By assembling the pangenome of B. napus, we show that 38% of the genes display PAV behaviour, with some of these variable genes predicted to be involved in important agronomic traits including flowering time, disease resistance, acyl lipid metabolism and glucosinolate metabolism. This study is a first and provides a detailed characterization of the association between HEs and PAVs in B. napus at the pangenome level. 相似文献