共查询到19条相似文献,搜索用时 78 毫秒
1.
2.
Clustal W—蛋白质与核酸序列分析软件 总被引:2,自引:1,他引:2
蛋白质与核酸的序列分析在现代生物学和生物信息学中发挥着重要作用,新的算法和软件层出不穷,本文介绍一个可运行在PC机上的完全免费的多序列比较软件-ClustalW,它不但可以进行蛋白质与核酸的多序列比较,分析不同序列之间的相似性关系,还可以绘制进化树。由于其灵活的输入输出格式、方便的参数设定和选择、详尽的在线帮助以及良好的可移植性,使得ClustalW在蛋白质与核酸的序列分析中得到了广泛应用。 相似文献
3.
4.
5.
在英特网上分析核酸与蛋白质的序列 总被引:4,自引:0,他引:4
在英特网上分析核酸与蛋白质的序列李健张卿伟(山东大学生命科学学院,济南250100)关键词英特网序列分析核酸蛋白质近年来,数目众多的分子生物学数据库和软件相继与英特网连接[1],使得在英特网上分析核酸与蛋白质序列成为可能,可分析的种类也越来越多。例如... 相似文献
6.
讨论了计算机序列分析的工作,介绍了基于WWW和UNIX的核酸序列分析实用软件,其特点是快速,易用,提高了工作效率。 相似文献
7.
通过电子邮件开展核酸和蛋白质序列分析 总被引:8,自引:1,他引:8
近年来,许多核酸和蛋白质序列数据库如GenBank、EMBL、DDBJ、SWISSPROT、PDB等均建立了与Internet的连接,并开通了电子邮件服务器,向用户免费提供序列分析服务。只要用户按规定的格式向电子邮件服务器发送序列分析请求,电子邮件服... 相似文献
8.
9.
本文介绍了计算机在核酸和蛋白质一级结构序列分析上的一些应用,包括序列的收集和贮存,两个或多个序列之间同源性的比较,用限制性内切酶找出酶切位点,找出DNA序列的开放式密码解读链和蛋白质序列倒翻成DMA序列的可能结果以及DNA和蛋白质序列的建立和应用等。 相似文献
10.
对侵染花生的黄瓜花叶病毒CA(CMVCA)株系进行克隆和序列分析。CMVCARNA1全长3356个核苷酸(nt),编码分子量为111kDa的1a蛋白;RNA2全长3045nt,编码分子量为96.7kDa的2a蛋白和13.1kDa的2b蛋白;RNA3全长2219nt,编码分子量为30.5kDa的3a蛋白和分子量为24kDa的外壳蛋白(CP)。序列同源性比较表明,CMVCARNA1、2、3与CMV亚组IACMVFny、亚组IBCMVSD、亚组IICMVQ株系序列同源性,RNA1分别为91.3%、91.1%和76.5%,RNA2分别为92.1%、90%和71.2%,RNA3分别为96.1%、92.6%和74.5%;与同属花生矮化病毒(Peanutstuntvirus,PSV)RNA1、2、3序列同源性分别为67.1%、58.2%和55.7%。上述研究未发现CMVCA基因组与PSVRNA链的重组,CMVCA对花生的侵染应是该株系适应于花生的遗传变异、长期进化的结果;对RNA35′NTR结构分析以及RNA35′NTR和CP系统进化树分析表明,CMVCA属CMVIB亚组。 相似文献
11.
对1989年春在河北保定分离的两株乙型流感病毒进行了抗原性、HA_1基因序列和种系发生分析,与不同期的国内外代表株比较结果表明,自1988年以来乙型流感病毒变异较快,B/河北/53/89株的HA_1基因序列与B/挪威/1/85株相比,其氨基酸的同源性为94.52%,与同期的B/香港/20/89株同源性为97.12%。种系发生分析结果,从1988至1989年乙型流感病毒出现了5个支系,同期在保定分离的两株病毒,在同源替代中分别属于两个支系。日本国基本每隔两年出现一次乙型流感的流行优势型,其发生频度与甲型流感相似。国内少见乙型的流行优势型,可能和使用的分离病毒材料有关,日本用MDCK细胞比国内用鸡胚对乙型流感病毒的分离阳性率高。 相似文献
12.
13.
Masato Ikegami Yoshinori Isomura Yoshinori Matumoto Masaaki Chatani Narinobu Inouye 《Microbiology and immunology》1995,39(12):995-1001
The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot virus Cy-1 strain (ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K, 183 K, 31 K, and 18 K proteins. Its genomic organization is similar to other tobamoviruses, TMV-V(vulgare), TMV-L (tomato strain), tobacco mild green mosaic virus (TMGMV) and cucumber green mottle mosaic virus (CGMMV). The 5′ non-coding regions of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The ORFs encoding the 126 K and 183 K proteins have 61% and 63% identities with those of TMV-V. The third ORF encoded a 31 K protein homologous to TMV cell-to-cell movement protein. It has 63% identities with that of TMV-V. The fourth ORF encoded an 18 K coat protein. The 5′ non-coding region, which extends from base 1 to 62 has 2 G residues and a ribosome binding site (AUU). The 3′ non-coding region, 414 nucleotides in length, is entirely different from that of other tobamoviruses. 相似文献
14.
Shao-hui MA Li-chun WANG Jian-sheng LIU Hai-jing SHI Long-ding LIU Qi-han LI 《Virologica Sinica》2010,(6)
The complete nucleotide sequence of the measles virus strain IMB-1,which was isolated in China,was determined.As in other measles viruses,its genome is 15,894 nucleotides in length and encodes six proteins.The full-length nucleotide sequence of the IMB-1 isolate differed from vaccine strains (including wild-type Edmonston strain) by 4%-5% at the nucleotide sequence level.This isolate has amino acid variations over the full genome,including in the hemagglutinin and fusion genes.This report is the first to de... 相似文献
15.
Nucleotide Sequence and Genetic Characterization of the Novel IncQ-like Plasmid pIE1107 总被引:3,自引:0,他引:3
Erhard Tietze 《Plasmid》1998,39(3):165-181
The analysis of the complete nucleotide sequence of the small resistance plasmid pIE1107 revealed a close similarity to the well-known IncQ plasmids. Highly conserved replication proteins and nearly identical origins of replication (oriV) suggest equivalent functions in the related replication systems. However, pIE1107 contains two copies of IncQ-oriV-like DNA which are slightly different regarding the iterons. Upon deletion of a silent copy of IncQ-oriV-like DNA the resulting plasmid is fully compatible with IncQ plasmids, indicating that there is no mutual communication between the replication control of the respective replicons. Experiments with clonedoriV DNA strongly suggest that the replication initiation protein of pIE1107 has specialized into the distinct target-iterons of its ownoriV which differs only by a few nucleotides from theoriV of IncQ plasmids. Implications from the apparent highly specific protein–DNA recognition and from the incompatibility properties of pIE1107 for the evolution of a family of compatible, IncQ-like plasmids are discussed. 相似文献
16.
17.
猪水泡病病毒全基因组核苷酸序列的测定与分析 总被引:4,自引:0,他引:4
猪水泡病是由猪水泡病病毒(Swine vesicular disease virus,SVDV)引起的猪的一种急性传染病,在症状上与口蹄疫极其相似。该病流行性强,发病率高,能造成严重的公共卫生问题。国际兽医局将其列为动物A类传染病,我国农业部列为动物一类传染病。SVDV属于小RNA病毒科肠道病毒属,其核酸类型为单股正链RNA分子,无囊膜,病毒基因组含一个大的开放阅读框,编码一条由2185个氨基酸组成的多聚蛋白。 相似文献
18.
Nucleotide Sequence Analysis of DNA 总被引:7,自引:0,他引:7
RAY WU 《Nature: New biology》1972,236(68):198-200
There are three major obstacles to the analysis of the nucleotide sequence in a DNA molecule starting from a known location in the DNA molecule. First, it is difficult to obtain large quantities of homogeneous DNA. Second, even the smallest DNA molecules contain several thousand nucleotides which make sequence analysis prohibitive. Third, there are no highly base-specific DNAases available for degrading DNA for sequence analysis. We have overcome some of these obstacles; first, by incorporating highly labelled deoxynucleotides into DNA in vitro, small amounts of material can be used for sequence analysis. Second, the nucleotide sequence of DNA molecules can now be determined from the 5′-terminal. Thus, two dodecanucleotide sequences corresponding to the two cohesive ends of λ DNA have been determined1 and a nona-decanucleotide sequence corresponding to one cohesive end of phage 186 DNA has been completed2. So far, our approach is limited to starting the analysis from the 5′-ends of a DNA molecule. A more general approach is being developed for starting the analysis from other selected parts of a DNA molecule with the use of specifically designed primers. 相似文献
19.
Mahfuzur R. Sarker Shigeru Akimoto Hideyo Ugai Tomomi Kuwahara Yoshinari Ohnishi 《Microbiology and immunology》1995,39(7):525-529
The complete nucleotide sequence of the gene (leuB) coding for β-isopropylmaiate dehydrogenase of Bacteroides fragilis was determined. An open reading frame of 1,061 nucleotides was detected that could encode a polypeptide of 353 amino acid residues with a calculated molecular mass of 39,179 Da. The deduced amino acid sequence of the β-isopropylmalate dehydrogenase from B. fragilis showed substantial sequence similarity with the β-isopropylmalate dehydrogenases from other bacteria. 相似文献