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1.
《Animal : an international journal of animal bioscience》2021,15(9):100345
One-third of beef calves fail to achieve adequate transfer of passive immunity (TPI) through timely ingestion of colostrum, which substantially increases their risk of preweaning morbidity and mortality. Two randomized clinical trials were designed to assess the impact of volume, immunoglobulin G (IgG) concentration, and feeding method of colostrum product on neonatal nursing behavior and TPI. In Trial 1, 47 calves were randomly assigned to receive one of three colostrum interventions by oro-esophageal tube feeder (OET): 1 L with 100 g/L IgG, 1.4 L with 70 g/L IgG, or 2 L with 100 g/L IgG. In Trial 2, 29 calves were randomly assigned to be fed 1 L of colostrum product with 100 g/L IgG by either nipple bottle (NB) or OET. Colostrum intervention (i.e. feeding of colostrum product) occurred within 60 minutes of birth. Cow-calf pairs were monitored by video surveillance in individual stalls for 24 h. Dam colostrum was collected at 10 minutes and calf serum was collected at 24–36 h after birth to assess IgG concentration. Differences among colostrum intervention groups on latency to stand and nurse were analyzed using Kaplan-Meier survival curves and Cox proportional hazard models. The impact of colostrum intervention group on TPI was assessed using multivariable linear regression modeling. In Trial 1, calves fed 1.4 L with 70 g/L IgG by OET nursed from their dams statistically significantly earlier compared to calves fed 1 L with 100 g/L IgG (P = 0.003) and calves fed 2 L with 100 g/L IgG (P = 0.008). Six of the 15 calves in the NB group in Trial 2 refused to consume part of the colostrum feeding offered by bottle and required follow-up tube feeding of the remaining volume. These calves were analyzed as a separate group (NB + OET). Calves fed 1 L by NB stood and nursed statistically significantly earlier than calves fed by OET (P = 0.005) or a combination of NB + OET (P = 0.003). Calf serum IgG concentrations were not statistically significantly different among colostrum intervention groups (P > 0.1). Overall, the colostrum interventions assessed in this study led to only one calf with failed TPI. While statistically significant differences in serum IgG concentrations were not detected in this study, subsequent nursing behavior did vary and was improved by feeding a moderate volume (1.4 L with 70 g/L IgG) of colostrum when using an OET, and by using the NB when feeding a smaller volume (1 L with 100 g/L IgG). 相似文献
2.
3.
《Animal : an international journal of animal bioscience》2016,10(10):1728-1733
Colostrum is an essential source of immunoglobulin G (IgG) for neonate piglets. However, colostrum IgG content and nutritional composition can vary considerably among sows due to age, parity, feeding regime and immunological background. Currently, there is no practical way to obtain information about colostrum IgG concentration at herd level. We evaluated sows’ colostrum IgG content on-farm using a Brix refractometer and its performance was compared with that of an IgG ELISA. In addition, nutritional compositions of the colostrum samples were analyzed using Fourier transform IR spectroscopy. Colostrum samples (5 to 6 ml) (n=153) were obtained within 0 to 3 h of farrowing. However, to obtain a 24 h IgG profile for 11 sows, colostrum samples were collected at 0, 2, 4, 6, 8, 10, 16 and 24 h after farrowing. A 0.3 ml of freshly drawn colostrum sample was used for the on-farm measurement of Brix percentages using a digital refractometer shortly after collection. The remaining fractions of the samples were frozen and submitted to laboratory analysis for total IgG, using a commercially available pig IgG ELISA kit. For nutritional composition analysis, a 35 ml colostrum sample (n=34) was obtained immediately after birth of first piglet from the first three pairs of frontal teats. Colostrum concentrations of IgG averaged 52.03±30.70 mg/ml (mean±SEM) at 0 to 3 h after farrowing. Concentration of IgG decreased on average by 50% during the 1st day of lactation (P<0.01). Sow parity did not influence colostrum concentrations of IgG. Differences in colostrum composition were recorded between two herds and among the parity groups (P<0.05). The Brix refractometer measurement of colostrum and the corresponding log transformed IgG measurements from the ELISA were moderately correlated (r=0.63, P<0.001, n=153). Based on the classification we suggest here, low levels of IgG (14.5±1.8 mg/ml) were recorded for colostrum samples with Brix readings below 20%. Borderline colostrum IgG content (43.8±2.3 mg/ml) had Brix readings of 20% to 24%, adequate colostrum IgG content (50.7±2.1 mg/ml) had Brix % readings of 25% to 29% and very good IgG colostrum content (78.6±8.4 mg/ml) had Brix readings >30%. Colostrum IgG concentration is highly variable among sows, Brix measurement of a sows’ fresh colostrum is an inexpensive, rapid and satisfactorily accurate method of estimating IgG concentration, providing indication of differentiation between good and poor IgG content of colostrum. 相似文献
4.
《Small Ruminant Research》2008,76(2-3):204-209
An experiment was conducted to examine the changes in colostral production and composition in Karakul ewes fed at different nutritional levels. Pregnant ewes were randomly allotted to one of four groups (n = 20 per group) 90 days after artificial insemination. Three groups were fed with total mixed rations supplying 90, 100 and 110% of the NRC requirements, respectively, and the last group served as the control feeding on natural vegetations until parturition. Blood samples were taken from the jugular vein during late pregnancy, and soon after lambing. Fifteen singleton ewes from each group were hand-milked completely at 1, 12 and 24 h post-lambing. Samples of colostrum were obtained and the lambs were fed with their ewe's colostrum (30 g/kg BW) at each milking. Blood samples were taken from the lambs before receiving their first colostrum, and again at 24 and 48 h post-lambing. Ewes on 110% NRC diet produced significantly (P < 0.01) more colostrum than did the ewes in other groups. Feeding level did not have a significant effect on the IgG level of the colostrums and blood sera of ewes and lambs. Mean IgG concentration in the ewe serum was greatest during late gestation (1–2 months before lambing) and were significantly reduced at 1 h post-lambing. Non-immunoglobulin composition of colostrum was not significantly affected by the treatment. There was a significant correlation between IgG levels of the ewe serum and colostrum (r = 0.64, P < 0.0001). Mean weight of the ewes on the control ration at lambing and the mean birth weight of their lambs were significantly lower as compared with other dietary groups. The results suggested that hand-feeding of Karakul ewes on poor rangeland vegetation with 110% NRC for 2 months before parturition increased colostral production, decreased the ewe loss and increased lamb birth weight which is beneficial to lamb survival. 相似文献
5.
Thirty-four pregnant crossbred beef cows were injected with prostaglandin F(2) alpha (PGF group, n = 11), dexamethasone (DEX group, n = 11), or saline (control group, n = 12) on Day 270 of gestation. Immediately after calving, all colostrum was milked from each cow. A sample was taken, and the remainder was fed to that cow's calf within one hour of birth. Serum was collected from each calf at 0 and 24 h of age. Immunoglobulin G (IgG) content of colostrum and serum was determined with commercial radial immunodiffusion plates. The data from four PGF cows that did not calve until after 140 h post injection were excluded from the results. Mean (+/- SD) volumes (ml) of colostrum were 2086 (+/-1148.4) for the PGF group, 1336 (+/-583.7) for the DEX group, and 2404 (+/-1140.7) for the control group. Mean (+/- SD) concentrations (mg/dl) of IgG in colostrum were 6017 (+/-3351.2) for PGF, 10285 (+/-5370.7) for DEX and 10766 (+/-5098.3) for the control group. Mean (+/- SD) total quantities of IgG (g) in colostrum were 133.9 (+/-120.03) for PGF, 134.1 (+/-96.67) for DEX and 235.6 (+/-147.22) for the control. IgG concentrations were very low or were not detectable in serum of all calves prior to administration of colostrum. Mean (+/- SD) concentrations (mg/dl) of IgG in serum of calves at 24 h of age were 1469 (+/-905.8) for calves from PGF cows, 1819 (+/-1289.8) for calves from DEX cows, and 3317 (+/-1888.2) for calves from control cows. Calves from control cows had significantly more IgG at 24 h than calves from PGF cows or DEX cows (p<0.05). Calves born to cows induced to calve early may be at an increased risk of failure of passive transfer and so should be monitored for IgG concentrations. 相似文献
6.
Honglong Zhu Yongxin Yang Tao Wu Yunxia Qi Dongwei Huang Rongwei Han Sheng Chen Jishun Tang Man Ren Xiaowei Zhao 《Animal : an international journal of animal bioscience》2022,16(8):100592
The contribution of colostrum to passive immunity transfer and intestinal protection in newborn ruminants is well known; however, it is currently unclear how colostrum intake affects intestinal innate immunity. We investigated the effects of bovine colostrum intake on ileal morphology, expression of genes involved in intestinal innate immunity, and serum concentrations of inflammatory cytokines in newborn lambs. Twenty-seven newborn male Hu lambs were used, of which 18 were bottle-fed either bovine colostrum (C24h; n = 9) or bovine mature milk (M24h; n = 9) within the first 2 h after birth at an intake of approximately 8% of BW; the remaining nine lambs did not receive any feeding (N24h). Blood and ileal tissue samples were collected after the lambs were slaughtered at 24 h after birth. Ileal villus height and villus height-to-crypt depth ratio were significantly higher in C24h than those in N24h and M24h lambs (P < 0.01). Messenger RNA (mRNA) abundance of toll-like receptor (TLR)-2, TLR3, TLR4, TLR6, TLR7, TLR8 and tumour necrosis factor alpha in the ileum was lower in C24h than that in N24h lambs (P < 0.05). Moreover, C24h lambs had a lower TLR3 mRNA abundance (P < 0.01) and a trend of lower TLR6 (P = 0.06) and interleukin 1 beta (P = 0.08) expression compared with those in M24h lambs. We also observed strong positive correlations of tumour necrosis factor alpha expression with that of TLR2 (r = 0.71; P < 0.001), TLR4 (r = 0.88; P < 0.001) and TLR8 (r = 0.83; P < 0.001). Interestingly, the expression of barrier-related molecules, including mucin-13, lysozyme, claudin (CLDN)-1, CLDN2, CLDN4, CLDN7, CLDN12, occludin, zonula occluden-1 and junctional adhesion molecule-1, was consistently lower in C24h lambs than that in N24h and M24h lambs (P < 0.05). These results indicated that the beneficial roles of colostrum intake on intestinal protection in newborn lambs were associated with low TLR expression, which was reflected by improved intestinal development and reduced inflammatory response. Further studies using fluorescence in situ hybridisation and immunohistochemical methods are needed to further explore the mechanisms underlying the lower expression of intestinal barrier-related molecules due to colostrum feeding. 相似文献
7.
Sauter SN Ontsouka E Roffler B Zbinden Y Philipona C Pfaffl M Breier BH Blum JW Hammon HM 《American journal of physiology. Endocrinology and metabolism》2003,285(2):E252-E261
Glucocorticoids and colostrum feeding influence postnatal maturation of the somatotropic axis. We have tested the hypothesis that dexamethasone (Dexa) affects the somatotropic axis in neonatal calves dependent on colostrum intake. Calves were fed either with colostrum or with a milk-based formula (n = 14/group), and, in each feeding group, one-half of the calves were treated with Dexa (30 micro g. kg body wt-1. day-1). Pre- and postprandial blood samples were taken on days 1, 2, 4, and 5, and liver samples were taken on day 5 of life. Dexa increased insulin-like growth factor (IGF)-I, but decreased growth hormone (GH) and IGF-binding protein (IGFBP)-1 and -2 plasma concentrations and increased GH receptor (GHR) mRNA levels in liver. Dexa increased IGF-I mRNA levels only in formula-fed calves and increased hepatic GHR binding capacity, but only in colostrum-fed calves. Colostrum feeding decreased IGFBP-1 and -2 plasma concentrations and hepatic IGFBP-2 and -3 mRNA levels. In conclusion, Dexa and colostrum feeding promoted maturation of the somatotropic axis. Dexa effects partly depended on whether colostrum was fed or not. 相似文献
8.
Heather W. Neave Lori Vickers Douglas M. Veira 《Animal Feed Science and Technology》2013,179(1-4):133-137
An effective colostrum management programme is the most important factor in determining the health and survival of the neonatal calf. Commercially available colostrum replacers (CR) and colostrum supplements (CS) are an alternative to colostrum on farms that do not have an adequate, high-quality colostrum supply, or those farms that want to prevent transmission of disease from cow to calf. The present study aimed to obtain a high immunoglobulin (Ig), dried bovine colostrum product that could be used as a CR or CS for dairy calves. Dried whey was made from 6 batches of colostrum and an additional 6 batches were used to produce dried whey and curds. Dried whey had higher IgG concentration (P<0.05) and lactose (P<0.05), and less fat (P<0.05) compared to curds or the original colostrum. There was a strong linear relationship between initial colostrum IgG concentration and whey (R2 = 0.86) with approximately 0.45 of the initial colostral IgG residing in curd. The high IgG and the composition of colostrum whey powder suggests it could be an effective CS product for use with dairy calves. The high fat and IgG content of the curd by-product indicate that it might be a potential weaning supplement in piglets or even a product for human consumption. 相似文献
9.
《Animal : an international journal of animal bioscience》2021,15(7):100299
Immunoglobulins G (IgG), A (IgA), and M (IgM) represent 70–80% of total proteins in cattle colostrum and are essential for the passive transfer of antibodies from the dam to the calf. Considering the practical difficulties of colostrum sample collection and the high cost of analysis, non-genetic sources of variation of the three immunoglobulins fractions have been scarcely studied together on a large scale in dairy cows. In the present study, IgG, IgA, IgM, and bovine serum albumin (BSA) were determined in colostrum samples of Holstein cows through bovine-specific radial immunodiffusion kits; such phenotypes allowed to investigate the effects of parity, herd, and calving season, and interactions. Only the first colostrum was considered in the present study, as the calf was separated from the dam immediately after birth and was not allowed to suckle. The average of IgG (n = 676), IgA (n = 573), IgM (n = 658), total immunoglobulins (n = 525), and BSA (n = 614) was 91.31, 4.20, 105.99, 5.05, and 2.47 g/L, respectively, and all traits positively correlated to each other. Overall, the immunoglobulins were less concentrated in colostrum of first- and second-parity cows than later-parity cows. These findings suggest that colostrum quality, based on Ig, is overall greater in cows that experienced more than two lactations, likely due to a greater experience of the immune system and to a wider immune heritage compared to younger cows. As regards the effect of calving season, the concentration of all Ig tended to be generally greater in colostrum sampled from August to November. Moreover, there were differences in IgG, IgA, and IgM concentration among the nine herds involved. Future studies will investigate the relationships of these traits with yield, and gross and detailed composition of bovine colostrum and will consider their genetic background to evaluate potential selection strategies to improve colostrum quality. 相似文献
10.
《Animal : an international journal of animal bioscience》2018,12(5):1041-1049
Despite the well-known importance of an adequate colostral immunoglobulin (Ig) transfer to calf health and survival, failed transfer of passive immunity (FTPI) remains a widespread problem in dairy farming. The aim of this study was to investigate the management factors associated with FTPI in newborn calves, evaluating particularly the combined effect of delivery time, amount and quality of the first colostrum meal. The study was conducted from March to August 2014 on 21 Italian dairy farms. Farmers were asked as first to answer a farm-level questionnaire on calf management. Blood sampling was then performed on overall 244 calves (1 to 5 days of age) born from Holstein cows, and a sample of the first colostrum meal of each calf was collected. Individual information on calves and the respective colostrum management were recorded. Serum and colostrum Ig concentrations were assessed by electrophoresis. A mixed effects multivariable logistic regression model was used to investigate the association of the variables obtained from both the management questionnaire and the individual calf data with FTPI (calf serum Ig concentration <10.0 g/l). A cumulative colostrum management score (CMS) that considered delivery time, amount and quality of the first colostrum meal was generated for 236 calves, with higher values indicating better colostrum management. Overall, 41.0% of the calves were found having FTPI, and within-farm percentage of FTPI was over 20.0% in 71.4% of the farms. The risk of having FTPI was higher both for Holstein purebred calves compared with Holstein-beef crossbreds and for females compared with males. Moreover, it increased by 13% with every hour of delay of the first colostrum meal provision since birth, whereas it decreased by 59% and 3%, respectively, with every additional liter of colostrum given and every additional gram of Ig per liter contained in the colostrum fed. Calf serum Ig concentration varied significantly according to the CMS, increasing by 1.53 g/l with every additional CMS point. In order to completely avoid FTPI, calves should receive at least 2.5 l of high-quality colostrum (Ig concentration >87.6 g/l) within 1.0 h from birth. Considerable improvements are still needed about colostrum management for newborn calves in dairy farms. The results of this study will help in developing farm-specific programs for reducing the occurrence of FTPI. 相似文献
11.
《Saudi Journal of Biological Sciences》2023,30(7):103701
Our aim was to compare the health and performance of ad libitum (ADLIB) and restrictedly fed Holstein Friesian heifer calves. Calves were selected to ADLIB (n = 13) and control (n = 13) groups randomly. The period of ADLIB feeding lasted for 3 weeks after colostrum supplementation. The calves in the control group received the same milk replacer, which was supplied according to the restrained feeding schedule of the farm. There was no difference between the two groups in weight, weight gain or movement activity, furthermore in the values of glucose, albumin, total protein, BHB, AST, oxidant and antioxidant status incl. dROM, PAT and OSI. The IL8 gene had higher levels (non significant, p > 0.05) of expression in the ADLIB group during the first 20 days of life, which indicates that ADLIB feeding might potentiate a stronger immune response to environmental stress. The IGF1 gene showed increased expression in the ADLIB fed group at almost all time points, however the difference was already detected on the first day of the study, indicating the importance of individual differences even within the same breed. During the first 10 days INS expression was higher in the restricted group, followed by a shift by day 20 and after, when the ADLIB group showed a higher relative expression level. The observed values describe a trend that, although not significant (p > 0.05), would seem to indicate that ADLIB feeding might potentiate a stronger immune response to environmental stress. 相似文献
12.
《Animal : an international journal of animal bioscience》2021,15(12):100403
Colostrum and milk are the first nutrient sources for newborn piglets. In addition, n-3 fatty acids (FAs) and their oxygenated derivatives (oxylipins) have the capacity to modulate immune components. The aim of the current study was to include a fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in sow diets to promote an increase of anti-inflammatory molecules in colostrum and milk to benefit piglets. Thirty-six sows were randomly assigned from insemination to the end of lactation to either a control diet with animal fat (15 g/kg in gestation and 30 g/kg in lactation) or an n-3 diet in which animal fat was totally (gestation) or half (lactation) replaced by an equivalent amount of solid fish oil. Performance of sows and piglets was monitored during the study. Colostrum and milk samples were obtained after the birth of the first piglet and at weaning, respectively. From all samples (n = 18 per treatment), FAs were quantified by gas chromatography and immunoglobulins and cytokines by ELISA. Three samples per treatment were randomly selected to analyse oxylipin composition by liquid chromatography-tandem mass spectrometry. In colostrum and in milk, the n-3 FA (P = 0.020 and P < 0.001), particularly EPA (P < 0.001 and P < 0.001) and DHA (P < 0.001 and P < 0.001), and also their oxygenated derivatives were increased in samples from sows fed n-3 diet. Fish oil had no effect on immunoglobulin concentrations, but reduced tumour necrosis factor α (TNFα) (P = 0.011) and a tendency to reduce interleukin 10 (IL10) (P = 0.059) were observed in milk. In conclusion, fish oil in sow diets increased n-3 FA, particularly EPA and DHA, and their oxygenated derivatives in colostrum and milk, reducing TNFα and IL10 in milk. 相似文献
13.
《Animal : an international journal of animal bioscience》2019,13(8):1599-1606
Resin acid-enriched composition (RAC) mainly containing tall oil fatty acid with an active component of resin acid (RA) can improve the microbial population in the digestive system, change the microbial fermentation, and improve the feed conversion ratio. We investigated the effects of dietary supplementation of RAC on sow colostrum yield (CY), colostrum composition and gut microbiota. Tall oil fatty acid and RA are commonly termed RAC and CLA, pinolenic, abietic, dehydrobiotic acids are characteristic components of RAC. The experiment was conducted in three trials in three respective herds. Sows were fed with a control diet and the same diet supplemented with 5 g RAC/day per sow during the last week of gestation. The 16S ribosomal RNA gene sequencing technique was used to assess sows’ faecal microbiota populations at farrowing. Colostrum nutritional composition, acute phase proteins (APPs) and immunoglobulin (Ig) content were also assessed. Individual piglets were weighed at birth and 24 h after the birth of first piglets in order to calculate CY and later at 3 to 4 weeks to calculate average daily gain. The RAC-fed sows had significantly higher IgG levels (P<0.05) in all three herds but treatment did not influence colostrum IgA and IgM concentration. There were no significant differences in colostrum protein, lactose and fat content in sows of the two diet groups (P>0.05), but those fed RAC had higher levels of colostrum serum amyloid A. Colostrum yield was significantly higher in RAC-fed sows in herds 2 and 3 with heavier piglets between 3 and 4 weeks of age (P<0.05), but not in herd 1 (P>0.05). Resin acid-enriched composition supplementation significantly increased some beneficial and fermentative bacteria (Romboutsia and Clostridium sensu stricto) than the control diet (P<0.01) while some opportunistic pathogens (Barnesiella, Sporobacter, Intestinimonas and Campylobacter), including Proteobacteria, were suppressed. Therefore, RAC added to the sow diet at late pregnancy increases colostrum IgG, colostrum availability for neonate piglets, and seems to promote better maternal intestinal microbial sources. 相似文献
14.
《Animal Feed Science and Technology》1997,67(4):291-298
Colostrum was collected from the first postpartum milking of German Black Pied cows. Four independent pools of colostrum were made and the following preservation methods replicated in each pool, viz. formaldehyde treatment, 0.1% (F1) and 0.05% (F2); formic acid treatment, 0.5% (FA1) and 0.1% (FA2) and an untreated control (NF). All the colostrum batches were stored at an average incubation temperature of 28°C in 200-ml plastic bottles. Samples were collected from every batch on Day 0 (before incubation) and subsequently after every week for 4 weeks. All the samples collected were analysed for immunoglobulin (IgG1, IgG2, IgA and IgM) content of the whey fraction using the single radial immunodiffusion (SRID) method. pH was measured using a glass electrode pH meter.Formaldehyde treatment of colostrum maintained almost constant immunoglobulin levels under the conditions of this experiment. There were significant drops in the mean IgG1 (P < 0.0001) and IgM (P < 0.005) contents in the control (NF) and the formic acid treated (FA1 and FA2) colostrum. The levels of IgA and IgG2 remained fairly constant for all treatments and there was no observable trend with storage duration. The pH of formaldehyde treated colostrum remained above 4.8 for the 4 weeks of storage whereas that of the untreated control colostrum dropped to below pH 4.8 in the first 3 days and remained stable to the 4th week. This work has shown that inclusion of formaldehyde at levels as low as 0.05% (wt/vol.) preserves immunoglobulins of colostrum stored at high ambient temperature. The use of formic acid was not beneficial for preservation of colostral immunoglobulins. Thus colostrum preserved with formaldehyde may be of good feeding value for newborn calves whereas that preserved with formic acid may be useful only for older calves. 相似文献
15.
Fernando T. Borderas Anne Marie B. de Passill Jeff Rushen 《Applied animal behaviour science》2009,120(1-2):56-61
Low temperatures increase mortality of calves but we know little of calves’ abilities to thermoregulate. To examine effects of milk feeding level on thermoregulatory behaviour, 27 Holstein calves were housed for 3 d after birth in individual pens fitted with heat lamps at one end to provide a thermal gradient. They were fed milk at a high (30% BW) or low (8% BW) daily allowance. Daily temperatures in the barn were 6.27 ± 3.12 °C, range: −5.46 °C to 16.63 °C. Temperature loggers were fitted to each calf to record ambient temperatures chosen by the calf. Video cameras recorded the calf's distance from the heat lamp and lying posture (on sternum or side, with legs extended or contracted) for 24 h/d, and records were scored every 20 min. Calves spent most time in the portion of the pen with heat lamps (Proc Mixed P < 0.05) but there was no effect of feeding level (P > 0.10). Calves tended to be closer to the heat lamp during the coldest periods of the day than during the warmer periods (P < 0.10). The difference between the temperature recorded in the barn and the temperature recorded on the calf's back was correlated with distance from the heat lamp (r = 0.32, P = 0.01) showing that the temperature logger on the calf reflected the ambient temperature chosen. Time spent in different lying postures was not affected by feeding level or barn temperature (P > 0.10). Young calves show a preference for warmer environments but this preference does not interact with the amount of milk fed. Temperature recorders attached to the calves can measure calves’ thermal preferences. Calves did not thermoregulate through changes in resting posture. 相似文献
16.
Analyses of biological databases such as those of genome, proteome, metabolome etc., have given insights in organization of biological systems. However, current efforts do not utilize the complete potential of available metabolome data. In this study, metabolome of bacterial systems with reliable annotations are analyzed and a simple method is developed to categorize pathways hierarchically, using rational approach. Ninety-four bacterial systems having for each ≥ 250 annotated metabolic pathways were used to identify a set of common pathways. 42 pathways were present in all bacteria which are termed as Core/Stage I pathways. This set of pathways was used along with interacting compounds to categorize pathways in the metabolome hierarchically. In each metabolome non-interacting pathways were identified including at each stage. The case study of Escherichia coli O157, having 433 annotated pathways, shows that 378 pathways interact directly or indirectly with 41 core pathways while 14 pathways are noninteracting. These 378 pathways are distributed in Stage II (289), Stage III (75), Stage IV (13) and Stage V (1) category. The approach discussed here allows understanding of the complexity of metabolic networks. It has pointed out that core pathways could be most ancient pathways and compounds that interact with maximum pathways may be compounds with high biosynthetic potential, which can be easily identified. Further, it was shown that interactions of pathways at various stages could be one to one, one to many, many to one or many to many mappings through interacting compounds. The granularity of the method discussed being high; the impact of perturbation in a pathway on the metabolome and particularly sub networks can be studied precisely. The categorizations of metabolic pathways help in identifying choke point enzymes that are useful to identify probable drug targets. The Metabolic categorizations for 94 bacteria are available at http://115.111.37.202/mpe/. 相似文献
17.
《Animal : an international journal of animal bioscience》2021,15(12):100397
Bovine respiratory disease is the leading user of antibiotics (AB) in calf production. Mycoplasma (M.) bovis could lead to greater use of AB as it is a persistent and AB resistant causative agent for respiratory diseases. Two cross-sectional studies were set up to assess the effects of lot size and feeding system on M. bovis infection and the effects of M. bovis seroconversion, lot size and feeding system on AB use in calves’ feedlots. Twenty-six lots in 22 fattening farms were monitored for 41–81 days, from all-in entry of calves until three consecutive weeks without using any collective antibiotics. M. bovis spread was estimated by measuring seroconversion at entry and at the end of study period in 10–15 calves randomly sampled in each lot. All AB treatments used in the meanwhile were recorded. The lots were selected according to feeding system, i.e. individual bucket (n = 7) vs. automated milk feeder (AMF, n = 19), and lot size (30–519 calves), less than 50 calves (n = 9) vs. more than 50 calves (n = 17). Statistical analysis was performed using multivariable generalised linear models with fattening farms as random effect. M. bovis spread increased with lot size (odds ratio (OR) 2.9[1.4; 5.8] per two-fold increase in lot size). This proportion of seroconverted calves was lower in bucket-fed lots compared to lots fed with the AMF using a shared nipple (OR = 0.03[0.003; 0.41]). The main risk factor for AB use was the lot size, with an increase of 1.5[0.94; 1.98] treatments per two-fold increase in lot size. For same size lots, the use of bucket can decrease AB consumption by up to 1.03[−2.18; 0.14] treatments per calf compared to AMF. Analysis of the association between seroconversion to M. bovis and AB use was inconclusive. We found that bucket feeding in small-size lots, i.e. up to a maximum of 50 calves in the same space, limits seroconversion to M. bovis and enables lower use of AB in veal calf production. 相似文献
18.
Annette Friedrich Mathias Büttner Günter Rademacher Wolfgang Klee Bianca K Weber Matthias Müller Annette Carlin Aryan Assad Angela Hafner-Marx Carola M Sauter-Louis 《BMC veterinary research》2011,7(1):10
Background
Since 2006, cases of haemorrhagic diathesis in young calves have been observed with a much higher incidence than previously known. The syndrome, now uniformly called Bovine Neonatal Pancytopenia (BNP), is characterized by multiple (external and internal) haemorrhages, thrombocytopenia, leukocytopenia, and bone marrow depletion. Although various infectious and toxicological causes of bleeding disorders in calves have been ruled out, the aetiology of BNP remains unknown. However, field observations have led to the hypothesis that the aetiological principle may be transmitted to calves via colostrum. 相似文献19.
Efficacy of hyperimmune bovine colostrum for prophylaxis of cryptosporidiosis in neonatal calves 总被引:17,自引:0,他引:17
Twelve neonatal calves were experimentally infected with oocysts of Cryptosporidium parvum. Six calves in group A fed hyperimmune colostrum at birth had significantly less diarrhea and shed oocysts for less time than did 6 calves in group B fed colostrum from cows that were not hyperimmune. Calves in group A had diarrhea for 0-4 days (means = 2.3 days), whereas calves in group B had diarrhea for 4-6 days (means = 5.0 days). Calves in group A shed oocysts for 4-9 days (means = 6.2 days), whereas calves in group B shed oocysts for 7-11 days (means = 8.5 days). These findings indicate that passive lacteal immunity conferred partial protection against cryptosporidiosis. Whether such protection was provided by the immunoglobulins that were highly elevated in the colostrum (greater than 1:200,000 for IgG1, IgM, and IgA) and constituted a large part of the circulating antibody in the calves, or by other biologically active factors, such as cytokines, is undetermined. 相似文献
20.
《Animal : an international journal of animal bioscience》2018,12(11):2300-2309
Oak acorn contains high levels of tannins which have capacity to form complexes with proteins and consequently reduce their availability and as a result it could reduce colostrum quality and immunoglobulin (Ig) synthesis. Thus, the aim of this experiment was to investigate the effects of dietary oak (Quercus persica) acorn (OA) level during the last 60 days of pregnancy on colostrum composition and plasma metabolites and IgG level of goats and their kids. In all, 24 multiparous pregnant goats (41.7±2.3 kg BW) were assigned into one of the three experimental diets consisted of control diet (without OA) and diets containing 20% (OA20) or 40% (OA40) OA on dry matter basis. Goats fed OA40 had lower BW change compared with other groups (P⩽0.05). Kids birth weight was not affected by experimental diets (P>0.05). Goats fed OA containing diets had lower plasma glucose, triglyceride and Fe concentrations compared with those fed the control diet (P<0.01). Plasma IgG (P<0.01) and urea (P<0.05) concentrations were lower in goats fed OA40 compared with others. Animals fed OA containing diets had higher plasma alanine aminotransferase than those fed the control diet (P<0.05). Goats fed OA40 produced colostrum with lower fat (P⩽0.05) and lactose (P<0.01) contents compared with other animals. Goats fed OA containing diets had lower colostrum IgG level than those fed the control diet (P⩽0.05). Kids plasma total protein concentration was not affected by experimental diets (P>0.05), whereas kids born from goats fed OA containing diets had lower plasma IgG level compared with the control diet (P<0.01). It is concluded that feeding OA during the last 60 days of pregnancy decreased colostrum quality which may have adverse effect on kid’s survival. 相似文献