青春双歧杆菌酸奶临床疗效的研究

本文报告了青春双歧杆菌酸奶对腹泻病儿的临床疗效,共有62例,随机分为两组,观察组32例,对照组30例。结果证明,3天治愈率观察组为90.6％,对照组为70％,经统计学处理有显著差异(P<0.05),本文研究证实,双歧杆菌酸奶对非感染性及感染性腹泻均有疗效,且可避免应用抗生素的副作用。另外,这种酸奶营养丰富,食用方便,可作为婴儿、老年人保健食品推广应用。     

双歧杆菌多元蛋白酸奶的免疫调节和抑瘤作用的研究

目的 :通过观察双歧杆菌多元蛋白酸奶对S1 80 荷瘤小鼠瘤组织、脾细胞代谢水平及吞噬功能的影响以及志愿者体内IL 6、TNF α、IFN γ生成的影响 ,探讨含青春双歧杆菌酸奶的免疫调节和抑瘤作用机制。方法 :采用含青春双歧杆菌酸奶灌胃S1 80 荷瘤小鼠 1ml/(天·只 ) ,连续 1 0d ,测量肿瘤大小 ,脾重、脾指数 ,计算抑瘤率 ,取脾细胞中性红法测脾细胞吞噬功能 ,MTT法测脾细胞增殖能力。自愿者口服酸奶 30 0ml(d·人 ) ,连续 1 5d ,用酶标试剂盒测量IL 6、TNF α、IFN γ三种细胞因子水平。结果 :含青春双歧杆菌酸奶对小鼠S1 80 肉瘤有明显的抑制作用 ;对志愿者IL 6、TNF α、IFN γ的生成有明显的促进作用。结论 :含青春双歧杆菌酸奶有免疫调节和抑瘤作用     

新型婴儿双歧杆菌马铃薯酸奶的工艺化初步研究

目的 以马铃薯和牛奶为主要原料,利用婴儿双歧杆菌进行发酵,制备一款新型酸奶。方法 采用婴儿双歧杆菌作为发酵菌株,发酵马铃薯牛奶。进行马铃薯酸奶原料的比例、酸奶发酵的温度和时间的探索。结果 当发酵温度42℃、发酵时间20 h、马铃薯汁10%时,酸奶的感官及口味较好。结论 利用10%马铃薯汁,牛奶等可初步制备出新型马铃薯汁酸奶。     

直投式酸奶发酵剂中双歧杆菌的分离

介绍一种简便可行的从直投式酸奶发酵荆中分离双歧杆菌的方法。发酵剂在液体培养基中活化后,在不同选择培养基上划线分离,根据菌落及菌体形态确定双歧杆菌。然后进行初步鉴定。     

双歧杆菌凝固型无糖酸奶生产工艺研究

对双歧杆菌凝固型无糖酸奶的生产工艺进行了研究。采用接种双歧杆菌、保加利亚乳杆菌和嗜热链球菌3菌株进行发酵。确定最佳工艺条件为双歧杆菌发酵剂接种量5%、木糖醇6%于39℃条件下培养8h后,再接入接种比例为1:1、接种量为4%的保加利亚乳杆菌、嗜热链球菌混合发酵剂,于42℃条件下培养3 h。在此工艺条件下生产的酸奶风味纯正,凝乳状态良好,口感细腻,具有天然的奶香味。     

双歧豆奶菌种的驯化研究

选用青春双歧杆菌在 A、B、C三种培养基上进行耐氧和凝固驯化 ,经 2 8代筛选出一株耐氧性并在 C培养基上进行耐氧和凝固驯化 ,经 2 8代筛选出一株耐氧性并在 C培养基中由 5 6 h可发生凝固 ,最后缩短到 12 h发生凝固的青春双歧杆菌 ;在 PYG和豆乳培养基中进行耐酸驯化 ,经 15代筛出一株能在p H4.7°T90的发酵豆乳中存活的青春双歧杆菌 ;在 BCPYC液体和固体培养基中进行耐热驯化 ,经 14代筛选出一株能在 47℃± 1℃的环境中生长的青春双歧杆菌。本菌在改良的 NPNL双歧选择培养基中生长 ,形态、培养与生化特性与原菌种无差异。     

菊芋在脱脂乳中对双歧杆菌生长的影响研究

以菊芋粉为材料,以长双歧杆菌(Blm)和两歧双歧杆菌(Bbm)为实验菌株,研究菊芋在脱脂乳中对双歧杆菌生长的影响,以期为进一步开发菊芋资源和研制双歧杆菌酸奶提供理论依据。结果表明在脱脂乳中加入3%的菊芋粉,凝乳时间有所缩短,在接种Blm的脱脂乳中缩短了0．9h,在接种Bbm的脱脂乳中缩短了1．1h。在脱脂乳中添加3%的菊芋粉后,明显促进了菌体产酸。     

双歧杆菌双元蛋白酸奶中特定菌含量及酸度的检测

目的 :检测双歧杆菌双元蛋白酸奶中发酵前后以及在贮藏期间的双歧杆菌和乳酸菌活菌数及酸度的变化。方法 :应用改良MRS琼脂平板法检测含菌量 ,应用吉尔涅尔度表示酸度 (°T)。结果 :双歧杆菌双元蛋白酸奶中双歧杆菌含量为 3 1× 10 7CFU/ml,乳酸菌含量为 4 1× 10 8CFU/ml;°T为 6 5± 5。随着贮藏进程的延长 ,特定菌的含量随贮藏期进程而下降 ,第 4～ 5天下降明显 ;°T随贮藏期延长而逐渐上升 ,保质期内°T为 75～ 110之间。结论 :双歧双元蛋白酸奶符合酸奶国家GB2 74 6～ 1999标准。     

16SrRNA荧光定量PCR法检测双歧杆菌

目的应用16SrRNA序列设计引物定量测定双歧杆菌。方法应用青春型双歧杆菌2627号作常规PCR扩增出的模板经系列稀释做荧光定量PCR,制作标准曲线;对青春型、长型、短型、婴儿型、两歧型、链型等6个种共15株双歧杆菌和大肠杆菌等10株其他细菌做荧光定量PCR,计算机通过与标准曲线比较给出定量结果;对青春型双歧杆菌2627号进行敏感性测定。结果15株双歧杆菌(10     

海藻糖对双歧杆菌DM8504菌株冷冻干燥保护效果的研究

本文就海藻糖对ＤＭ８５０４双歧杆菌的冻干保护效果进行了研究,考察了海藻糖浓度、保护剂溶液与菌泥混合时间对ＤＭ８５０４冻干存活率的影响,结果证实该保护剂对ＤＭ８５０４双歧杆菌有良好的冻干保护效果,优于其它保护剂。     

刺梨活性冻干粉冷冻干燥工艺研究

研究了刺梨汁的真空冷冻干燥工艺,得到其冻干曲线,测定了刺梨汁的共融点,确定经济合理的装料量和工艺参数.根据本研究工艺参数真空冷冻干燥刺梨汁,较好的保持了SOD的活性.     

Inheritable nature of enological quantitative traits is demonstrated by meiotic segregation of industrial wine yeast strains

Wine yeast strains exhibit a wide variability in their technological properties. The large number of allelic variants and the high degree of heterozygosity explain this genetic variability found among the yeast flora. Furthermore, most enological traits are controlled by polygenic systems presenting complex interactions between the alleles. Taking this into account, we hypothesized that the meiotic segregation of such alleles from a given strain might generate a progeny population with very different technological properties. In this work, a population of 50 progeny clones derived from four industrial wine strains of Saccharomyces cerevisiae was characterized for three major enological traits: ethanol tolerance, volatile-acidity production and hydrogen sulphide production. For this purpose, reliable laboratory fermentation tests were developed in accordance with enological practice. A wide variability in the values of the various parameters was found among spore clones obtained after sporulation. Many clones presenting better aptitudes than the parental strains were obtained. Moreover, analysis of the progeny demonstrated that: (1) traits are in part inheritable; (2) traits are clearly polygenic; (3) broad relations of dominance/recessivity can be established. All these findings constitute an initial step for establishing breeding strategies for wine yeast improvement.     

高密度发酵和真空冷冻干燥工艺对乳酸菌抗冷冻性的影响

经真空冷冻干燥得到的乳酸菌发酵剂存活率和后期的低温贮藏稳定性与诸多因素相关。本文综述了制备乳酸菌发酵剂过程中高密度发酵和真空冷冻干燥工艺的不同对乳酸菌抗冷冻性的影响,其中高密度发酵过程中的培养基组分、培养温度、发酵恒定pH、中和剂的选择、菌体收获时期和发酵结束后处理以及真空冷冻干燥过程中保护剂的添加、预冷冻处理等是影响乳酸菌抗冷冻性的重要因素。通过对这些相关因素的综述分析,为提高乳酸菌发酵剂的冻干存活率和后期的低温贮藏稳定性提供新的思路,且应用抗冷冻性强、活力高的乳酸菌发酵剂对有效提高乳制品的质量和企业的经济效益意义重大。     

S-腺苷-L-蛋氨酸(SAM)稳定性盐产品制备的研究

研究了S-腺苷-L-蛋氨酸(SAM)盐产品的制备方法,确定了甲醇沉淀结晶SAM硫酸盐的最优条件,进一步优化了脱色精制SAM双盐产品的方法。破胞液经超滤后,过D152树脂以硫酸溶液洗脱,可得到纯度(色谱纯)在98%以上的SAM硫酸盐产品,以对甲苯磺酸溶液溶解SAM硫酸盐后脱色精制得到SAM硫酸对甲苯磺酸双盐。     

Optimization of reversed-phase microcapillary liquid chromatography for quantitative proteomics

A specific capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) method for the determination of serotonin (5HT) and its precursors tryptophan (Trp) and 5-hydroxytryptophan (5HTP) in human platelet rich plasma is described. The analytes were removed from the plasma samples and preconcentrated by solid phase extraction (SPE) on mixed mode cation-exchange sorbents. The SPE recoveries were 71.6 +/- 3.1 for 5HT, 91.0 +/- 2.8 for Trp, and 95.3 +/- 5.9% for 5HTP. Deuterated analogues of 5HT and Trp were used as internal standards for quantitation purposes. Submicromolar detection limits were obtained for standard mixtures of all compounds and their deuterated isotopes, except 5HTP, which had detection limits in the low micromolar range. The potential usefulness of this method in the clinical setting was demonstrated by analyzing plasma extracts from healthy volunteers as well as from pathological samples. While 5HTP was not present in any of the analyzed samples, the levels of 5HT and Trp in both normal and pathological plasma were determined.     

High-coverage quantitative proteomics using amine-specific isotopic labeling

Peptide dimethylation with isotopically coded formaldehydes was evaluated as a potential alternative to techniques such as the iTRAQ method for comparative proteomics. The isotopic labeling strategy and custom-designed protein quantitation software were tested using protein standards and then applied to measure proteins levels associated with Alzheimer's disease (AD). The method provided high accuracy (10% error), precision (14% RSD) and coverage (70%) when applied to the analysis of a standard solution of BSA by LC-MS/MS. The technique was then applied to measure protein abundance levels in brain tissue afflicted with AD relative to normal brain tissue. 2-D LC-MS analysis identified 548 unique proteins (p<0.05). Of these, 349 were quantified with two or more peptides that met the statistical criteria used in this study. Several classes of proteins exhibited significant changes in abundance. For example, elevated levels of antioxidant proteins and decreased levels of mitochondrial electron transport proteins were observed. The results demonstrate the utility of the labeling method for high-throughput quantitative analysis.